Prupe.3G308100_v2.0.a1

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0014782
Physical Location & Seq
Reverse (-)
26849613 .. 26852330
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G308100.2

Sequence Viewer

Length: 1281 bp
ATGTGTTTTTCTTCTTCACTGCCTAAGCCTTGTTTGTGTGTCTCTCTCTCGTCTCTAGGAAAGGAGGAGATTAGAGAAGAAGGCAGAGTTGTTGTTGTTGTGGTTATGGAGTGGGACTTTAAGGACTTTGCTTGTTGGGATTCAACCGATGAGCTGGAGTTGGAGTTGGAGTTGGAGTTGGAATTGGAGCAGCAAAAACTGGAACAAGATAGTGACCTTGCTGCTTTGGTTGGGTCCATCTCACAACCCCAGAAGAAGAAGAGATCAGCAGCTGCTGGATCTTTACTAGTGGATTTGAACTTGTCTGATTCTCCTCCTGCTGGACACTTGTTCAAGGACCCAATTAAGCCCTCAGCTGTGGCGTCATCATCGTCACCGTTATCCTCACCATCATCTTCATCATCATCATCCAAAAGGGTTAAAAGTAATGGAACTGATGAGCAGAAATTGTCATGCTTGGTTGAGGGTTGCAAGGCGGACCTCAGTCTTTGCAGGGAGTATCACCGACGCCATAGAGTCTGTGAGCTCCACTCTAAGACCCCTGTTGTCTCCGTCAGGGGTGAACAGAAACGGTTCTGCCAGCAGTGCAGCAGATTCCATTCTCTGGTGGAGTTTGATGAGGTCAAGAGAAGCTGCAGGAAACGTCTCAACGGTCACAACCAGCGCAGGAGGAAGCCTAAACCAGAATCCCTCTACTTCACTTCCAACTTCCTGTCCGGTTACAAAGGTCCCAGAATCTTGCAATTTAGTAACCCGCAGCTATGTGCAACCAATAATGTGAGAAGCATGTGGCCTGTGGAAGCCAAAAATGGAGCAGAATCCATGCTTTACAGCCGCCATCCTTCATTCAATGGAGGAGAAAAACCATTTCTTTTCTTGCAGACAAATAATGACACTAAAGCTGCTTCAGGCAACCGGGCAGCCCCTCAAACTTTCTTGCGCCACCAGCCATTTCCAACAACTGCTGCCTCACCCGCAGAAAGCAGAAAGGGCAGTCATATAATGTCTTCTGATGGTATGACTCGAGTCATTGACTCGGGTTGTGCTCTCTATCTTCTGTCATCACATCCAGCACAAGCTTCAGTGACTCAGTCCAATGTCACTACGACTCATCCAGGTCAGCTCCTAGACTCGAGACTTCAGAAGAACGGCAGTTTTGCAGAATTCTCATGCTCACATGATGCGGAGGACAAGGCTGCCAATCCAGAAATGATGTTTCTTATGGGGCCTGATGGCTTTTTGGACAATGGGGTGCAAATGCTTCCAATTTTATTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

427

Amino Acids

47.05

Weight (kDa)

7.9

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014782)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g53760
malus_domestica MD09G1008900.v1.1 MD17G1001400.v1.1
prunus_persica Prupe.3G308100_v2.0.a1 Prupe.3G308100_v2.0.a1
pyrus_communis pycom111g00720 pycom17g00760
rosa_chinensis RchiOBHm_Chr2g0175901
rosa_laevigata RLG00000022362
rosa_multiflora Rmu_ssc0000114.1_g000004
rosa_roxburghii Rroxscaffold_2G00076970
rosa_rugosa Rorug02G0588300
rosa_samantha Rh2AG667600 Rh2BG679100 Rh2CG641900 Rh2DG692600
rosa_wichuraiana Rw2G054720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 604
AciI CCGC 5 cut(s) 476, 755, 835, 975, 1184
AclWI GGATC 1 cut(s) 286
AcsI RAATTY 1 cut(s) 1163
AcuI CTGAAG 3 cut(s) 891, 1065, 1124
AcyI GRCGYC 2 cut(s) 362, 508
AfiI CCNNNNNNNGG 2 cut(s) 320, 604
AgsI TTSAA 4 cut(s) 144, 298, 334, 850
AhlI ACTAGT 1 cut(s) 286
AjnI CCWGG 1 cut(s) 1114
AluBI AGCT 9 cut(s) 154, 272, 356, 526, 633, 760, 902, 1079, 1123
AluI AGCT 9 cut(s) 154, 272, 356, 526, 633, 760, 902, 1079, 1123
Alw21I GWGCWC 2 cut(s) 528, 1048
Alw26I GTCTC 5 cut(s) 46, 57, 553, 650, 1129
AlwI GGATC 1 cut(s) 286
AlwNI CAGNNNCTG 2 cut(s) 272, 275
Ama87I CYCGRG 3 cut(s) 1023, 1036, 1132
AoxI GGCC 2 cut(s) 791, 1226
ApoI RAATTY 1 cut(s) 1163
Asp700I GAANNNNTTC 1 cut(s) 572
AspLEI GCGC 2 cut(s) 666, 942
AspS9I GGNCC 5 cut(s) 234, 337, 478, 728, 1226
AsuC2I CCSGG 1 cut(s) 917
AsuHPI GGTGA 5 cut(s) 366, 378, 494, 572, 963
AvaI CYCGRG 3 cut(s) 1023, 1036, 1132
AvaII GGWCC 4 cut(s) 234, 337, 478, 728
BanII GRGCYC 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 999
Bbv12I GWGCWC 2 cut(s) 528, 1048
BbvCI CCTCAGC 1 cut(s) 352
BccI CCATC 5 cut(s) 245, 397, 846, 1007, 1226
BceAI ACGGC 1 cut(s) 1165
BciT130I CCWGG 1 cut(s) 1116
BcnI CCSGG 1 cut(s) 917
BcoDI GTCTC 5 cut(s) 46, 57, 553, 650, 1129
BcuI ACTAGT 1 cut(s) 286
BfaI CTAG 3 cut(s) 56, 287, 1127
BfmI CTRYAG 1 cut(s) 634
Bme1390I CCNGG 2 cut(s) 917, 1116
Bme18I GGWCC 4 cut(s) 234, 337, 478, 728
BmeT110I CYCGRG 3 cut(s) 1023, 1036, 1132
BmgT120I GGNCC 5 cut(s) 234, 337, 478, 728, 1226
BmiI GGNNCC 4 cut(s) 235, 339, 730, 1227
BmrFI CCNGG 2 cut(s) 917, 1116
BmsI GCATC 1 cut(s) 1171
BoxI GACNNNNGTC 2 cut(s) 483, 1025
BpiI GAAGAC 1 cut(s) 999
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 2 cut(s) 24, 352
BpuMI CCSGG 1 cut(s) 917
BsaHI GRCGYC 2 cut(s) 362, 508
BsaWI WCCGGW 1 cut(s) 716
BsaXI ACNNNNNCTCC 6 cut(s) 149, 155, 161, 179, 185, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 604
Bse1I ACTGG 1 cut(s) 204
BseBI CCWGG 1 cut(s) 1116
BseGI GGATG 4 cut(s) 407, 838, 1066, 1111
BseLI CCNNNNNNNGG 2 cut(s) 320, 604
BseMII CTCAG 3 cut(s) 366, 496, 1103
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 3 cut(s) 80, 303, 870
BsgI GTGCAG 1 cut(s) 607
BshFI GGCC 2 cut(s) 793, 1228
BsiHKAI GWGCWC 2 cut(s) 528, 1048
BsiHKCI CYCGRG 3 cut(s) 1023, 1036, 1132
BsiSI CCGG 2 cut(s) 717, 916
BslFI GGGAC 2 cut(s) 128, 714
BslI CCNNNNNNNGG 2 cut(s) 320, 604
BsmAI GTCTC 5 cut(s) 46, 57, 553, 650, 1129
BsmBI CGTCTC 2 cut(s) 57, 650
BsmFI GGGAC 2 cut(s) 128, 714
BsnI GGCC 2 cut(s) 793, 1228
BsoBI CYCGRG 3 cut(s) 1023, 1036, 1132
Bsp1286I GDGCHC 2 cut(s) 528, 1048
Bsp143I GATC 2 cut(s) 263, 278
BspACI CCGC 5 cut(s) 476, 755, 835, 975, 1184
BspANI GGCC 2 cut(s) 793, 1228
BspCNI CTCAG 3 cut(s) 365, 495, 1102
BspLI GGNNCC 4 cut(s) 235, 339, 730, 1227
BspMAI CTGCAG 1 cut(s) 638
BspPI GGATC 1 cut(s) 286
BsrI ACTGG 1 cut(s) 204
BssMI GATC 2 cut(s) 263, 278
BssNI GRCGYC 2 cut(s) 362, 508
Bst2UI CCWGG 1 cut(s) 1116
Bst4CI ACNGT 3 cut(s) 378, 573, 653
Bst6I CTCTTC 1 cut(s) 254
BstACI GRCGYC 2 cut(s) 362, 508
BstC8I GCNNGC 1 cut(s) 581
BstDEI CTNAG 5 cut(s) 24, 352, 482, 534, 1089
BstF5I GGATG 4 cut(s) 407, 838, 1066, 1111
BstHHI GCGC 2 cut(s) 666, 942
BstKTI GATC 2 cut(s) 266, 281
BstMAI GTCTC 5 cut(s) 46, 57, 553, 650, 1129
BstMBI GATC 2 cut(s) 263, 278
BstMWI GCNNNNNNNGC 4 cut(s) 585, 946, 974, 990
BstNI CCWGG 1 cut(s) 1116
BstNSI RCATGY 1 cut(s) 790
BstPAI GACNNNNGTC 2 cut(s) 483, 1025
BstSCI CCNGG 2 cut(s) 915, 1114
BstSFI CTRYAG 1 cut(s) 634
BstV2I GAAGAC 1 cut(s) 999
BstX2I RGATCY 1 cut(s) 278
BstYI RGATCY 1 cut(s) 278
BsuRI GGCC 2 cut(s) 793, 1228
BtsCI GGATG 4 cut(s) 407, 838, 1066, 1111
BtsI GCAGTG 2 cut(s) 17, 590
BtsIMutI CAGTG 3 cut(s) 17, 590, 1089
Cac8I GCNNGC 1 cut(s) 581
CaiI CAGNNNCTG 2 cut(s) 272, 275
CfoI GCGC 2 cut(s) 666, 942
Cfr13I GGNCC 5 cut(s) 234, 337, 478, 728, 1226
CseI GACGC 2 cut(s) 351, 516
CviAII CATG 5 cut(s) 453, 787, 823, 1170, 1178
DdeI CTNAG 5 cut(s) 24, 352, 482, 534, 1089
DpnI GATC 2 cut(s) 265, 280
DpnII GATC 2 cut(s) 263, 278
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
EciI GGCGGA 1 cut(s) 491
Ecl136II GAGCTC 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 4 cut(s) 234, 337, 478, 728
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 3 cut(s) 891, 1065, 1124
Eco88I CYCGRG 3 cut(s) 1023, 1036, 1132
EcoICRI GAGCTC 1 cut(s) 526
EcoO109I RGGNCCY 3 cut(s) 337, 728, 1226
EcoRI GAATTC 1 cut(s) 1163
EcoRII CCWGG 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 528
Esp3I CGTCTC 2 cut(s) 57, 650
FaeI CATG 5 cut(s) 456, 790, 826, 1173, 1181
FaqI GGGAC 2 cut(s) 128, 714
FatI CATG 5 cut(s) 452, 786, 822, 1169, 1177
FauI CCCGC 2 cut(s) 762, 982
FokI GGATG 4 cut(s) 394, 825, 1053, 1098
FriOI GRGCYC 1 cut(s) 528
FspBI CTAG 3 cut(s) 56, 287, 1127
GlaI GCGC 2 cut(s) 665, 941
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 2 cut(s) 793, 1228
HapII CCGG 2 cut(s) 717, 916
HgaI GACGC 2 cut(s) 351, 516
HhaI GCGC 2 cut(s) 666, 942
Hin1I GRCGYC 2 cut(s) 362, 508
Hin1II CATG 5 cut(s) 456, 790, 826, 1173, 1181
Hin6I GCGC 2 cut(s) 664, 940
HinP1I GCGC 2 cut(s) 664, 940
HindIII AAGCTT 1 cut(s) 1077
HpaII CCGG 2 cut(s) 717, 916
HphI GGTGA 5 cut(s) 366, 378, 494, 572, 963
Hpy166II GTNNAC 1 cut(s) 563
Hpy188I TCNGA 3 cut(s) 307, 1012, 1143
Hpy188III TCNNGA 3 cut(s) 625, 1134, 1205
Hpy8I GTNNAC 1 cut(s) 563
Hpy99I CGWCG 1 cut(s) 510
HpyAV CCTTC 2 cut(s) 74, 852
HpyCH4III ACNGT 3 cut(s) 378, 573, 653
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 9 cut(s) 471, 492, 588, 636, 742, 767, 880, 1160, 1255
HpyF10VI GCNNNNNNNGC 4 cut(s) 585, 946, 974, 990
HpyF3I CTNAG 5 cut(s) 24, 352, 482, 534, 1089
HpySE526I ACGT 1 cut(s) 643
Hsp92I GRCGYC 2 cut(s) 362, 508
Hsp92II CATG 5 cut(s) 456, 790, 826, 1173, 1181
HspAI GCGC 2 cut(s) 664, 940
Kzo9I GATC 2 cut(s) 263, 278
LmnI GCTCC 4 cut(s) 187, 531, 812, 1128
LweI GCATC 1 cut(s) 1171
MaeI CTAG 3 cut(s) 56, 287, 1127
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 7 cut(s) 212, 372, 653, 719, 749, 1084, 1099
MalI GATC 2 cut(s) 265, 280
MboI GATC 2 cut(s) 263, 278
MflI RGATCY 1 cut(s) 278
MhlI GDGCHC 2 cut(s) 528, 1048
MluCI AATT 6 cut(s) 182, 342, 446, 743, 1163, 1266
MlyI GAGTC 7 cut(s) 525, 1015, 1028, 1035, 1081, 1102, 1124
MmeI TCCRAC 6 cut(s) 141, 147, 153, 159, 729, 980
MroXI GAANNNNTTC 1 cut(s) 572
MseI TTAA 3 cut(s) 120, 345, 420
MspA1I CMGCKG 2 cut(s) 272, 356
MspI CCGG 2 cut(s) 717, 916
MspR9I CCNGG 2 cut(s) 917, 1116
MvaI CCWGG 1 cut(s) 1116
MwoI GCNNNNNNNGC 4 cut(s) 585, 946, 974, 990
NciI CCSGG 1 cut(s) 917
NdeII GATC 2 cut(s) 263, 278
NlaIII CATG 5 cut(s) 456, 790, 826, 1173, 1181
NlaIV GGNNCC 4 cut(s) 235, 339, 730, 1227
NmuCI GTSAC 5 cut(s) 212, 372, 653, 1084, 1099
NspI RCATGY 1 cut(s) 790
PaeR7I CTCGAG 2 cut(s) 1023, 1132
PdmI GAANNNNTTC 1 cut(s) 572
PfeI GAWTC 6 cut(s) 140, 308, 594, 686, 735, 818
PflFI GACNNNGTC 1 cut(s) 1090
PflMI CCANNNNNTGG 1 cut(s) 604
PleI GAGTC 7 cut(s) 524, 1015, 1028, 1034, 1081, 1102, 1124
PpsI GAGTC 7 cut(s) 524, 1015, 1028, 1034, 1081, 1102, 1124
PpuMI RGGWCCY 2 cut(s) 337, 728
PshAI GACNNNNGTC 2 cut(s) 483, 1025
Psp124BI GAGCTC 1 cut(s) 528
Psp5II RGGWCCY 2 cut(s) 337, 728
Psp6I CCWGG 1 cut(s) 1114
PspGI CCWGG 1 cut(s) 1114
PspN4I GGNNCC 4 cut(s) 235, 339, 730, 1227
PspPI GGNCC 5 cut(s) 234, 337, 478, 728, 1226
PspPPI RGGWCCY 2 cut(s) 337, 728
PspXI VCTCGAGB 1 cut(s) 1023
PstI CTGCAG 1 cut(s) 638
PstNI CAGNNNCTG 2 cut(s) 272, 275
PsuI RGATCY 1 cut(s) 278
PsyI GACNNNGTC 1 cut(s) 1090
PvuII CAGCTG 2 cut(s) 272, 356
SacI GAGCTC 1 cut(s) 528
SaqAI TTAA 3 cut(s) 120, 345, 420
Sau3AI GATC 2 cut(s) 263, 278
Sau96I GGNCC 5 cut(s) 234, 337, 478, 728, 1226
SchI GAGTC 7 cut(s) 525, 1015, 1028, 1035, 1081, 1102, 1124
ScrFI CCNGG 2 cut(s) 917, 1116
SduI GDGCHC 2 cut(s) 528, 1048
SfaNI GCATC 1 cut(s) 1171
SfcI CTRYAG 1 cut(s) 634
Sfr274I CTCGAG 2 cut(s) 1023, 1132
SinI GGWCC 4 cut(s) 234, 337, 478, 728
SlaI CTCGAG 2 cut(s) 1023, 1132
SmlI CTYRAG 2 cut(s) 1023, 1132
SmoI CTYRAG 2 cut(s) 1023, 1132
SpeI ACTAGT 1 cut(s) 286
Sse9I AATT 6 cut(s) 182, 342, 446, 743, 1163, 1266
SsiI CCGC 5 cut(s) 476, 755, 835, 975, 1184
SspMI CTAG 3 cut(s) 56, 287, 1127
SstI GAGCTC 1 cut(s) 528
StyD4I CCNGG 2 cut(s) 915, 1114
TaaI ACNGT 3 cut(s) 378, 573, 653
TaiI ACGT 1 cut(s) 646
TaqI TCGA 2 cut(s) 1024, 1133
TasI AATT 6 cut(s) 182, 342, 446, 743, 1163, 1266
TauI GCSGC 1 cut(s) 837
TfiI GAWTC 6 cut(s) 140, 308, 594, 686, 735, 818
Tru1I TTAA 3 cut(s) 120, 345, 420
Tru9I TTAA 3 cut(s) 120, 345, 420
TscAI CASTG 3 cut(s) 24, 590, 1089
TseFI GTSAC 5 cut(s) 212, 372, 653, 1084, 1099
Tsp45I GTSAC 5 cut(s) 212, 372, 653, 1084, 1099
TspDTI ATGAA 2 cut(s) 387, 834
TspGWI ACGGA 1 cut(s) 541
TspRI CASTG 3 cut(s) 24, 590, 1089
Tth111I GACNNNGTC 1 cut(s) 1090
Van91I CCANNNNNTGG 1 cut(s) 604
VpaK11BI GGWCC 4 cut(s) 234, 337, 478, 728
XapI RAATTY 1 cut(s) 1163
XceI RCATGY 1 cut(s) 790
XhoI CTCGAG 2 cut(s) 1023, 1132
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 3 cut(s) 56, 287, 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.