Prupe.4G035900_v2.0.a1

Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0006238
Physical Location & Seq
Forward (+)
1696372 .. 1698582
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G035900.1

Sequence Viewer

Length: 630 bp
ATGGAGGTCAAGCTGGCGAATGACAAGCGTGAAAGAGAAATGTATGATAGTTTTGCTGAACTTTACGCCATTATAAAGACCACCGAGAAGCTTGAAAAGGCATATATTCGTGATGTGATCTCTTCGTCAGCGTATGAGACTGAATGCCAAAAGCTCATTGCCCACTTTAAGACTTTGGCTTCCACGCTTAAAGACACCATCCCAAGCATCGAGCGATTTGCAGAAACATACAAGATGGACTGCCCAGCAGCTATAAACCGCCTTGTGACCTCAGGGGTGCCTGCCACAGTGGAGCATCGGGCTGCTGCAGCTGCCTCCATGACTTCCTCAGCTGCTGCTGTGGCAGAATGCACACAGAATTTCATCACTGCAATGGACTCATTGAAGCTGAACATGGTTGCAGTTGATCAGGTGCACCCGCTGCTCTCGGACCTTTTAACGTCTCTGGGTAAGTTGACCTTTCTGCCACCAGACTTTTTGGGGAAGGTGAAGTTGAAGGAGTGGATTGCAAGATTATCAAAGATGGGAGCGGCCGATGAGTTGACAGAGCAGCAGTCTAGGCAGCTTCACTTTGATCTTGAGTCGTCCTACAACTCATTTATGGCAGCTTTGCCAAACTATGGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
VPS28

Protein Analysis

210

Amino Acids

23.18

Weight (kDa)

5.63

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 277
AccB7I CCANNNNNTGG 1 cut(s) 620
AccBSI CCGCTC 1 cut(s) 530
AciI CCGC 3 cut(s) 259, 419, 530
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 1 cut(s) 358
AfaI GTAC 1 cut(s) 625
AfiI CCNNNNNNNGG 1 cut(s) 620
AgsI TTSAA 3 cut(s) 95, 385, 496
AluBI AGCT 9 cut(s) 13, 91, 154, 251, 311, 332, 388, 565, 608
AluI AGCT 9 cut(s) 13, 91, 154, 251, 311, 332, 388, 565, 608
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 131, 447
Alw44I GTGCAC 1 cut(s) 413
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 1 cut(s) 531
ApaLI GTGCAC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 1 cut(s) 499
AvaII GGWCC 1 cut(s) 430
AxyI CCTNAGG 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 417
BbvCI CCTCAGC 1 cut(s) 328
BccI CCATC 3 cut(s) 206, 229, 517
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 2 cut(s) 131, 447
BfaI CTAG 1 cut(s) 558
BfmI CTRYAG 1 cut(s) 306
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 1 cut(s) 279
BmsI GCATC 2 cut(s) 216, 304
Bpu10I CCTNAGC 1 cut(s) 328
BpuEI CTTGAG 1 cut(s) 599
Bsc4I CCNNNNNNNGG 1 cut(s) 620
Bse21I CCTNAGG 1 cut(s) 271
Bse3DI GCAATG 2 cut(s) 156, 378
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 620
BseMI GCAATG 2 cut(s) 156, 378
BseMII CTCAG 2 cut(s) 285, 342
BseSI GKGCMC 1 cut(s) 417
BseX3I CGGCCG 1 cut(s) 531
BseYI CCCAGC 1 cut(s) 244
Bsh1285I CGRYCG 1 cut(s) 534
BshFI GGCC 1 cut(s) 533
BshNI GGYRCC 1 cut(s) 277
BsiEI CGRYCG 1 cut(s) 534
BsiHKAI GWGCWC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 620
BsmAI GTCTC 2 cut(s) 131, 447
BsmBI CGTCTC 1 cut(s) 447
BsmI GAATGC 2 cut(s) 149, 353
BsnI GGCC 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 3 cut(s) 117, 406, 574
BspACI CCGC 3 cut(s) 259, 419, 530
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 284, 341
BspLI GGNNCC 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 277
BsrBI CCGCTC 1 cut(s) 530
BsrDI GCAATG 2 cut(s) 156, 378
BssMI GATC 3 cut(s) 117, 406, 574
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 127
BstAPI GCANNNNNTGC 1 cut(s) 421
BstC8I GCNNGC 2 cut(s) 15, 282
BstDEI CTNAG 2 cut(s) 271, 328
BstF5I GGATG 1 cut(s) 198
BstKTI GATC 3 cut(s) 120, 409, 577
BstMAI GTCTC 2 cut(s) 131, 447
BstMBI GATC 3 cut(s) 117, 406, 574
BstMCI CGRYCG 1 cut(s) 534
BstMWI GCNNNNNNNGC 5 cut(s) 308, 311, 341, 421, 559
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 417
BstZI CGGCCG 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 271
BsuRI GGCC 1 cut(s) 533
BtsCI GGATG 1 cut(s) 198
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 2 cut(s) 294, 366
Cac8I GCNNGC 2 cut(s) 15, 282
CaiI CAGNNNCTG 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 430
Csp6I GTAC 1 cut(s) 624
CviAII CATG 2 cut(s) 319, 394
CviQI GTAC 1 cut(s) 624
DdeI CTNAG 2 cut(s) 271, 328
DpnI GATC 3 cut(s) 119, 408, 576
DpnII GATC 3 cut(s) 117, 406, 574
EaeI YGGCCR 1 cut(s) 531
EagI CGGCCG 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
EclXI CGGCCG 1 cut(s) 531
Eco47I GGWCC 1 cut(s) 430
Eco52I CGGCCG 1 cut(s) 531
Eco81I CCTNAGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 447
FaeI CATG 2 cut(s) 322, 397
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 2 cut(s) 318, 393
FauI CCCGC 1 cut(s) 426
FbaI TGATCA 1 cut(s) 406
FokI GGATG 1 cut(s) 185
FspBI CTAG 1 cut(s) 558
GsaI CCCAGC 1 cut(s) 248
HaeIII GGCC 1 cut(s) 533
Hin1II CATG 2 cut(s) 322, 397
HincII GTYRAC 2 cut(s) 456, 543
HindII GTYRAC 2 cut(s) 456, 543
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 377, 581
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 3 cut(s) 415, 456, 543
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 2 cut(s) 110, 578
Hpy8I GTNNAC 3 cut(s) 415, 456, 543
HpyAV CCTTC 2 cut(s) 478, 490
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 7 cut(s) 221, 308, 351, 371, 401, 415, 509
HpyF10VI GCNNNNNNNGC 5 cut(s) 308, 311, 341, 421, 559
HpyF3I CTNAG 2 cut(s) 271, 328
HpySE526I ACGT 1 cut(s) 440
Hsp92II CATG 2 cut(s) 322, 397
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 3 cut(s) 117, 406, 574
LmnI GCTCC 2 cut(s) 292, 527
LpnPI CCDG 6 cut(s) 258, 258, 294, 395, 431, 483
LweI GCATC 2 cut(s) 216, 304
MaeI CTAG 1 cut(s) 558
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 119, 408, 576
MbiI CCGCTC 1 cut(s) 530
MboI GATC 3 cut(s) 117, 406, 574
MboII GAAGA 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 1 cut(s) 358
MlyI GAGTC 2 cut(s) 371, 590
MnlI CCTC 3 cut(s) 280, 325, 337
MseI TTAA 3 cut(s) 168, 189, 437
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 3 cut(s) 311, 332, 421
Mva1269I GAATGC 2 cut(s) 149, 353
MwoI GCNNNNNNNGC 5 cut(s) 308, 311, 341, 421, 559
NdeII GATC 3 cut(s) 117, 406, 574
NlaIII CATG 2 cut(s) 322, 397
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 265
PctI GAATGC 2 cut(s) 149, 353
PflMI CCANNNNNTGG 1 cut(s) 620
PleI GAGTC 2 cut(s) 371, 589
PpsI GAGTC 2 cut(s) 371, 589
PsiI TTATAA 1 cut(s) 74
PspFI CCCAGC 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 430
PstI CTGCAG 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 335
PvuII CAGCTG 2 cut(s) 311, 332
RsaI GTAC 1 cut(s) 625
RsaNI GTAC 1 cut(s) 624
RseI CAYNNNNRTG 1 cut(s) 371
SaqAI TTAA 3 cut(s) 168, 189, 437
Sau3AI GATC 3 cut(s) 117, 406, 574
Sau96I GGNCC 1 cut(s) 430
SchI GAGTC 2 cut(s) 371, 590
SduI GDGCHC 1 cut(s) 417
SfaNI GCATC 2 cut(s) 216, 304
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 1 cut(s) 358
SsiI CCGC 3 cut(s) 259, 419, 530
SspMI CTAG 1 cut(s) 558
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 443
TaqI TCGA 1 cut(s) 210
TasI AATT 1 cut(s) 358
TauI GCSGC 1 cut(s) 533
Tru1I TTAA 3 cut(s) 168, 189, 437
Tru9I TTAA 3 cut(s) 168, 189, 437
TscAI CASTG 2 cut(s) 294, 373
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 352
TspRI CASTG 2 cut(s) 294, 373
Van91I CCANNNNNTGG 1 cut(s) 620
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 1 cut(s) 358
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.