pycom05g29870

Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006238
Physical Location & Seq
Forward (+)
30210667 .. 30211296
630 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29870.1

Sequence Viewer

Length: 630 bp
ATGGAGGTCAAGCTGGCCAATGACAAGCGTGAAAGAGAAATGTATGATAGTTTTGCCGAACTATACGCCATTATCAAGACCACCGAGAAGCTTGAGAAAGCGTATATTCGGGATGTGATCACTTCTTCTGCGTATGAGACCGAATGCCAAAAACTCATTGCCCACTTTAAGACATTGGCTTCCACGCTTAAGGACACCATCCCAAGCATTGAGCGGTTTGCAGATACATACAAGATGGACTGCCTAGCGGCTATAAACCGTCTTGTGACCTCAGGGGTGCCTGCCACAGTGGAGCACCGGGCTGCTGCAGCTTCCTCCATGACTTCCTCAGCTGCCGCTGTGGCAGAATGCACACAGAATTTCATCACTGCAATGGACTCCTTGAAGCTGAACATGGTGGCAGTCGATCAGGTGCACCCGCTGCTCTCGGACCTCTCAACGTCTCTTGGAAAGTTGACCTTTCTACCACCGGACTTTTTGGGGAAGGTAAAGCTGAAGGAGTGGATTGCAAGGCTATCGAAGATGGGAGCAGCTGATGAGTTGACGGAGCAGCAGTCCAGGCAGCTTCACTTCGATCTCGAATCATCATACAACTCGTTTATGGCGGCTTTGCCGAATTATGGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
VPS28

Protein Analysis

210

Amino Acids

23.19

Weight (kDa)

5.62

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS28 PF03997 18 - 204 5.7e-71 VPS28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 277
AccBSI CCGCTC 1 cut(s) 214
AciI CCGC 5 cut(s) 214, 248, 336, 419, 605
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 1 cut(s) 625
AfiI CCNNNNNNNGG 1 cut(s) 620
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 385
AjnI CCWGG 1 cut(s) 557
AluBI AGCT 8 cut(s) 13, 91, 311, 332, 388, 493, 533, 565
AluI AGCT 8 cut(s) 13, 91, 311, 332, 388, 493, 533, 565
Alw21I GWGCWC 2 cut(s) 297, 417
Alw26I GTCTC 2 cut(s) 131, 447
Alw44I GTGCAC 1 cut(s) 413
AoxI GGCC 1 cut(s) 15
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 8 cut(s) 302, 305, 308, 332, 421, 530, 550, 562
ApoI RAATTY 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 430
AsuC2I CCSGG 1 cut(s) 299
AvaII GGWCC 1 cut(s) 430
AxyI CCTNAGG 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 417
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 1 cut(s) 277
Bbv12I GWGCWC 2 cut(s) 297, 417
BbvCI CCTCAGC 1 cut(s) 328
BbvI GCAGC 8 cut(s) 289, 292, 319, 320, 408, 542, 562, 574
BccI CCATC 3 cut(s) 206, 229, 517
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BciT130I CCWGG 1 cut(s) 559
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 131, 447
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 1 cut(s) 306
BfrI CTTAAG 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 299, 559
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 2 cut(s) 299, 559
Bpu10I CCTNAGC 1 cut(s) 328
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 131
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 620
Bse21I CCTNAGG 1 cut(s) 271
Bse3DI GCAATG 2 cut(s) 156, 378
BseBI CCWGG 1 cut(s) 559
BseGI GGATG 2 cut(s) 118, 198
BseLI CCNNNNNNNGG 1 cut(s) 620
BseMI GCAATG 2 cut(s) 156, 378
BseMII CTCAG 2 cut(s) 285, 342
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 8 cut(s) 289, 292, 319, 320, 408, 542, 562, 574
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 1 cut(s) 277
BsiHKAI GWGCWC 2 cut(s) 297, 417
BsiSI CCGG 2 cut(s) 298, 470
BslI CCNNNNNNNGG 1 cut(s) 620
BsmAI GTCTC 2 cut(s) 131, 447
BsmBI CGTCTC 1 cut(s) 447
BsmI GAATGC 2 cut(s) 149, 353
BsnI GGCC 1 cut(s) 17
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 297, 417
Bsp143I GATC 3 cut(s) 117, 406, 574
BspACI CCGC 5 cut(s) 214, 248, 336, 419, 605
BspANI GGCC 1 cut(s) 17
BspCNI CTCAG 2 cut(s) 284, 341
BspLI GGNNCC 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 277
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 131
BsrBI CCGCTC 1 cut(s) 214
BsrDI GCAATG 2 cut(s) 156, 378
BssMI GATC 3 cut(s) 117, 406, 574
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 2 cut(s) 260, 289
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 421
BstC8I GCNNGC 2 cut(s) 15, 282
BstDEI CTNAG 2 cut(s) 271, 328
BstF5I GGATG 2 cut(s) 118, 198
BstKTI GATC 3 cut(s) 120, 409, 577
BstMAI GTCTC 2 cut(s) 131, 447
BstMBI GATC 3 cut(s) 117, 406, 574
BstMWI GCNNNNNNNGC 4 cut(s) 308, 341, 421, 559
BstNI CCWGG 1 cut(s) 559
BstSCI CCNGG 2 cut(s) 297, 557
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 417
BstV1I GCAGC 8 cut(s) 289, 292, 319, 320, 408, 542, 562, 574
Bsu36I CCTNAGG 1 cut(s) 271
BsuRI GGCC 1 cut(s) 17
BtsCI GGATG 2 cut(s) 118, 198
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 2 cut(s) 294, 366
Cac8I GCNNGC 2 cut(s) 15, 282
Cfr13I GGNCC 1 cut(s) 430
Csp6I GTAC 1 cut(s) 624
CviAII CATG 2 cut(s) 319, 394
CviQI GTAC 1 cut(s) 624
DdeI CTNAG 2 cut(s) 271, 328
DpnI GATC 3 cut(s) 119, 408, 576
DpnII GATC 3 cut(s) 117, 406, 574
EaeI YGGCCR 1 cut(s) 15
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 430
Eco57I CTGAAG 1 cut(s) 515
Eco81I CCTNAGG 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 557
Esp3I CGTCTC 1 cut(s) 447
FaeI CATG 2 cut(s) 322, 397
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 2 cut(s) 318, 393
FauI CCCGC 1 cut(s) 426
FbaI TGATCA 1 cut(s) 117
FokI GGATG 2 cut(s) 125, 185
FspBI CTAG 1 cut(s) 245
HaeIII GGCC 1 cut(s) 17
HapII CCGG 2 cut(s) 298, 470
Hin1II CATG 2 cut(s) 322, 397
HincII GTYRAC 2 cut(s) 456, 543
HindII GTYRAC 2 cut(s) 456, 543
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 377, 581
HpaII CCGG 2 cut(s) 298, 470
Hpy166II GTNNAC 3 cut(s) 415, 456, 543
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 3 cut(s) 76, 110, 578
Hpy8I GTNNAC 3 cut(s) 415, 456, 543
HpyAV CCTTC 2 cut(s) 478, 490
HpyCH4III ACNGT 2 cut(s) 260, 289
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 6 cut(s) 221, 308, 351, 371, 415, 509
HpyF10VI GCNNNNNNNGC 4 cut(s) 308, 341, 421, 559
HpyF3I CTNAG 2 cut(s) 271, 328
HpySE526I ACGT 1 cut(s) 440
Hsp92II CATG 2 cut(s) 322, 397
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 117, 406, 574
LmnI GCTCC 3 cut(s) 292, 527, 547
LpnPI CCDG 7 cut(s) 258, 294, 311, 395, 483, 544, 571
Lsp1109I GCAGC 8 cut(s) 289, 292, 319, 320, 408, 542, 562, 574
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 119, 408, 576
MbiI CCGCTC 1 cut(s) 214
MboI GATC 3 cut(s) 117, 406, 574
MboII GAAGA 2 cut(s) 117, 532
MhlI GDGCHC 2 cut(s) 297, 417
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 2 cut(s) 358, 616
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 1 cut(s) 371
MnlI CCTC 4 cut(s) 280, 325, 337, 443
Mox20I TGGCCA 1 cut(s) 17
MscI TGGCCA 1 cut(s) 17
MseI TTAA 2 cut(s) 168, 189
MslI CAYNNNNRTG 1 cut(s) 371
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 4 cut(s) 332, 338, 421, 533
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 2 cut(s) 298, 470
MspR9I CCNGG 2 cut(s) 299, 559
Mva1269I GAATGC 2 cut(s) 149, 353
MvaI CCWGG 1 cut(s) 559
MwoI GCNNNNNNNGC 4 cut(s) 308, 341, 421, 559
NciI CCSGG 1 cut(s) 299
NdeII GATC 3 cut(s) 117, 406, 574
NlaIII CATG 2 cut(s) 322, 397
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 265
PctI GAATGC 2 cut(s) 149, 353
PfeI GAWTC 1 cut(s) 581
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 557
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 430
PstI CTGCAG 1 cut(s) 310
PvuII CAGCTG 2 cut(s) 332, 533
RsaI GTAC 1 cut(s) 625
RsaNI GTAC 1 cut(s) 624
RseI CAYNNNNRTG 1 cut(s) 371
SaqAI TTAA 2 cut(s) 168, 189
Sau3AI GATC 3 cut(s) 117, 406, 574
Sau96I GGNCC 1 cut(s) 430
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 2 cut(s) 299, 559
SduI GDGCHC 2 cut(s) 297, 417
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 2 cut(s) 92, 188
SmoI CTYRAG 2 cut(s) 92, 188
Sse9I AATT 2 cut(s) 358, 616
SsiI CCGC 5 cut(s) 214, 248, 336, 419, 605
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 2 cut(s) 297, 557
TaaI ACNGT 2 cut(s) 260, 289
TaiI ACGT 1 cut(s) 443
TaqI TCGA 4 cut(s) 405, 518, 573, 579
TaqII GACCGA 1 cut(s) 155
TasI AATT 2 cut(s) 358, 616
TauI GCSGC 3 cut(s) 251, 338, 608
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 2 cut(s) 168, 189
Tru9I TTAA 2 cut(s) 168, 189
TscAI CASTG 2 cut(s) 294, 373
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 8 cut(s) 302, 305, 308, 332, 421, 530, 550, 562
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 352
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 2 cut(s) 294, 373
Vha464I CTTAAG 1 cut(s) 188
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 1 cut(s) 358
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.