Rorug04G0422800

Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003204
Physical Location & Seq
Forward (+)
58220095 .. 58228314
8220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0422800.1

Sequence Viewer

Length: 753 bp
ATGGGGAGTGATCATGACAATGGCTATGGTGACTTGGAGAAGCAGATGCTGGTTCTTCACGAACACGAAGAGAAGCACTTCATGTCCAGTGAGATTGTCCGTGACGTCATTCTCGGCGTCTCCGACGGTCTCACTGTCCCTTTTGCCCTCGCCGCGGGGTTGTCCGGCGCTGACGTCAGCTCCTCCATCATCCTCATTGCCGGCATTGCTGAGGTTGCGGCCGGTGCCATCTCCATGGGACTTGGCGGGTATCTTGCGGCGAAGAGCGAAGAAGATCATTACAAGAAAGAACTAAAGAGGGAGCAAGATGAAATCATCGCCGTCCCTGATATAGAGGCTGCTGAGGTTGCAGAAATATTGTCACAATATGGGGTTGAAGCACATGAATATGGGCCTGTGGTGAATGCTCTAAGAAGGAACCCTCAAGCCTGGCTCGATTTCATGATGAAATTTGAACTAGGACTCGACAAGCCAGACCCAATGAGAGCCCTGCAGAGTGCAATGACAATAGCCTTTTCATACGTCTGTGGTGGACTGGTGCCTCTCCTTCCCTACATGCTCATTCCAGTTGCCACCAAGGCGGTGCTTGCATCAGTGGTGGTCACCATAGTGGCATTGCTGATCTTCGGCTTTGCAAAGGGTCACTTCACCGGAACTCAACCTTTCAAGAGCGCTTTCCAAACCGCCTTGATAGGAGCCATCGCGTCCGCGGCTGCTTATTCCATTGCTAAACTCTTCCAATCCTACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
VPS28

Protein Analysis

250

Amino Acids

26.76

Weight (kDa)

5.02

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 33 - 243 6.6e-74 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 108, 177
AccB1I GGYRCC 2 cut(s) 224, 538
AccII CGCG 3 cut(s) 155, 704, 710
AciI CCGC 9 cut(s) 153, 155, 218, 246, 257, 581, 684, 708, 710
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 1 cut(s) 449
AcyI GRCGYC 3 cut(s) 105, 117, 174
AfeI AGCGCT 1 cut(s) 673
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 3 cut(s) 377, 455, 667
AjnI CCWGG 1 cut(s) 428
AleI CACNNNNGTG 1 cut(s) 608
AluBI AGCT 1 cut(s) 180
AluI AGCT 1 cut(s) 180
Alw26I GTCTC 2 cut(s) 124, 134
AlwNI CAGNNNCTG 1 cut(s) 49
Aor51HI AGCGCT 1 cut(s) 673
AoxI GGCC 2 cut(s) 219, 392
ApeKI GCWGC 2 cut(s) 338, 713
ApoI RAATTY 1 cut(s) 449
Asp700I GAANNNNTTC 1 cut(s) 77
AspLEI GCGC 2 cut(s) 170, 674
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 4 cut(s) 41, 412, 595, 640
BanI GGYRCC 2 cut(s) 224, 538
BanII GRGCYC 1 cut(s) 490
BbvCI CCTCAGC 2 cut(s) 210, 342
BbvI GCAGC 2 cut(s) 325, 700
BccI CCATC 3 cut(s) 194, 236, 707
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 1 cut(s) 430
BclI TGATCA 1 cut(s) 10
BcoDI GTCTC 2 cut(s) 124, 134
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 491
BfoI RGCGCY 2 cut(s) 171, 675
BglI GCCNNNNNGGC 1 cut(s) 578
BisI GCNGC 6 cut(s) 153, 219, 258, 339, 711, 714
BlsI GCNGC 6 cut(s) 154, 220, 259, 340, 712, 715
Bme1390I CCNGG 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 4 cut(s) 226, 419, 540, 697
BmrFI CCNGG 1 cut(s) 430
BmsI GCATC 2 cut(s) 36, 599
Bpu10I CCTNAGC 2 cut(s) 210, 342
BpuEI CTTGAG 1 cut(s) 408
BsaHI GRCGYC 3 cut(s) 105, 117, 174
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 4 cut(s) 153, 234, 576, 708
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse118I RCCGGY 2 cut(s) 200, 221
Bse1I ACTGG 3 cut(s) 87, 540, 566
Bse3DI GCAATG 5 cut(s) 195, 204, 507, 614, 723
BseBI CCWGG 1 cut(s) 430
BseDI CCNNGG 4 cut(s) 153, 234, 576, 708
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 5 cut(s) 195, 204, 507, 614, 723
BseMII CTCAG 2 cut(s) 201, 333
BseNI ACTGG 3 cut(s) 87, 540, 566
BseRI GAGGAG 1 cut(s) 172
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 2 cut(s) 325, 700
Bsh1236I CGCG 3 cut(s) 155, 704, 710
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 221, 394
BshNI GGYRCC 2 cut(s) 224, 538
BsiEI CGRYCG 1 cut(s) 222
BsiSI CCGG 4 cut(s) 165, 201, 222, 651
BslFI GGGAC 3 cut(s) 122, 252, 308
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 124, 134
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 3 cut(s) 122, 252, 308
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 2 cut(s) 221, 394
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 490
Bsp143I GATC 3 cut(s) 10, 274, 621
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 9 cut(s) 153, 155, 218, 246, 257, 581, 684, 708, 710
BspANI GGCC 2 cut(s) 221, 394
BspCNI CTCAG 2 cut(s) 202, 334
BspFNI CGCG 3 cut(s) 155, 704, 710
BspHI TCATGA 2 cut(s) 13, 441
BspLI GGNNCC 4 cut(s) 226, 419, 540, 697
BspMAI CTGCAG 1 cut(s) 495
BspQI GCTCTTC 1 cut(s) 257
BspT107I GGYRCC 2 cut(s) 224, 538
BspTNI GGTCTC 1 cut(s) 134
BsrDI GCAATG 5 cut(s) 195, 204, 507, 614, 723
BsrFI RCCGGY 2 cut(s) 200, 221
BsrI ACTGG 3 cut(s) 87, 540, 566
BssAI RCCGGY 2 cut(s) 200, 221
BssECI CCNNGG 4 cut(s) 153, 234, 576, 708
BssMI GATC 3 cut(s) 10, 274, 621
BssNI GRCGYC 3 cut(s) 105, 117, 174
BssT1I CCWWGG 2 cut(s) 234, 576
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 2 cut(s) 128, 136
Bst6I CTCTTC 3 cut(s) 63, 257, 740
BstACI GRCGYC 3 cut(s) 105, 117, 174
BstC8I GCNNGC 2 cut(s) 202, 588
BstDEI CTNAG 3 cut(s) 210, 342, 410
BstDSI CCRYGG 3 cut(s) 153, 234, 708
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 3 cut(s) 155, 704, 710
BstH2I RGCGCY 2 cut(s) 171, 675
BstHHI GCGC 2 cut(s) 170, 674
BstKTI GATC 3 cut(s) 13, 277, 624
BstMAI GTCTC 2 cut(s) 124, 134
BstMBI GATC 3 cut(s) 10, 274, 621
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 8 cut(s) 152, 206, 215, 224, 347, 578, 587, 710
BstNI CCWGG 1 cut(s) 430
BstNSI RCATGY 1 cut(s) 559
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 491
BstUI CGCG 3 cut(s) 155, 704, 710
BstV1I GCAGC 2 cut(s) 325, 700
BstXI CCANNNNNNTGG 1 cut(s) 235
BstZI CGGCCG 1 cut(s) 219
BsuRI GGCC 2 cut(s) 221, 394
BtgI CCRYGG 3 cut(s) 153, 234, 708
BtgZI GCGATG 2 cut(s) 301, 685
BtsCI GGATG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 94, 132, 600
Cac8I GCNNGC 2 cut(s) 202, 588
CaiI CAGNNNCTG 1 cut(s) 49
CciI TCATGA 2 cut(s) 13, 441
CfoI GCGC 2 cut(s) 170, 674
Cfr10I RCCGGY 2 cut(s) 200, 221
Cfr13I GGNCC 1 cut(s) 392
Cfr42I CCGCGG 2 cut(s) 156, 711
CseI GACGC 2 cut(s) 106, 693
CviAII CATG 6 cut(s) 14, 82, 235, 383, 442, 556
DdeI CTNAG 3 cut(s) 210, 342, 410
DpnI GATC 3 cut(s) 12, 276, 623
DpnII GATC 3 cut(s) 10, 274, 621
EaeI YGGCCR 1 cut(s) 219
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 3 cut(s) 63, 257, 740
EarI CTCTTC 3 cut(s) 63, 257, 740
EclXI CGGCCG 1 cut(s) 219
Eco130I CCWWGG 2 cut(s) 234, 576
Eco24I GRGCYC 1 cut(s) 490
Eco31I GGTCTC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 673
Eco52I CGGCCG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 601
EcoO65I GGTNACC 1 cut(s) 601
EcoRII CCWGG 1 cut(s) 428
EcoT14I CCWWGG 2 cut(s) 234, 576
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 2 cut(s) 234, 576
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 6 cut(s) 17, 85, 238, 386, 445, 559
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 3 cut(s) 122, 252, 308
FatI CATG 6 cut(s) 13, 81, 234, 382, 441, 555
FauI CCCGC 2 cut(s) 148, 239
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 6 cut(s) 153, 219, 258, 339, 711, 714
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 6 cut(s) 153, 219, 258, 339, 711, 714
FspBI CTAG 1 cut(s) 458
GlaI GCGC 2 cut(s) 169, 673
GluI GCNGC 6 cut(s) 153, 219, 258, 339, 711, 714
HaeII RGCGCY 2 cut(s) 171, 675
HaeIII GGCC 2 cut(s) 221, 394
HapII CCGG 4 cut(s) 165, 201, 222, 651
HgaI GACGC 2 cut(s) 106, 693
HhaI GCGC 2 cut(s) 170, 674
Hin1I GRCGYC 3 cut(s) 105, 117, 174
Hin1II CATG 6 cut(s) 17, 85, 238, 386, 445, 559
Hin6I GCGC 2 cut(s) 168, 672
HinP1I GCGC 2 cut(s) 168, 672
HinfI GANTC 1 cut(s) 462
HpaII CCGG 4 cut(s) 165, 201, 222, 651
HphI GGTGA 4 cut(s) 41, 412, 595, 640
Hpy166II GTNNAC 1 cut(s) 533
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 4 cut(s) 14, 59, 442, 667
Hpy8I GTNNAC 1 cut(s) 533
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 2 cut(s) 408, 557
HpyCH4III ACNGT 2 cut(s) 128, 136
HpyCH4IV ACGT 3 cut(s) 105, 174, 522
HpyCH4V TGCA 5 cut(s) 350, 493, 500, 590, 635
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 206, 215, 224, 347, 578, 587, 710
HpyF3I CTNAG 3 cut(s) 210, 342, 410
HpySE526I ACGT 3 cut(s) 105, 174, 522
Hsp92I GRCGYC 3 cut(s) 105, 117, 174
Hsp92II CATG 6 cut(s) 17, 85, 238, 386, 445, 559
HspAI GCGC 2 cut(s) 168, 672
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 10
KspI CCGCGG 2 cut(s) 156, 711
Kzo9I GATC 3 cut(s) 10, 274, 621
LguI GCTCTTC 1 cut(s) 257
LmnI GCTCC 3 cut(s) 185, 301, 695
Lsp1109I GCAGC 2 cut(s) 325, 700
LweI GCATC 2 cut(s) 36, 599
MaeI CTAG 1 cut(s) 458
MaeII ACGT 3 cut(s) 105, 174, 522
MaeIII GTNAC 5 cut(s) 29, 101, 360, 601, 641
MalI GATC 3 cut(s) 12, 276, 623
MboI GATC 3 cut(s) 10, 274, 621
MboII GAAGA 7 cut(s) 47, 80, 274, 281, 284, 616, 727
MhlI GDGCHC 1 cut(s) 490
MluCI AATT 1 cut(s) 449
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 9 cut(s) 158, 193, 203, 205, 291, 328, 337, 432, 552
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 77
MslI CAYNNNNRTG 4 cut(s) 18, 233, 387, 608
MspA1I CMGCKG 2 cut(s) 155, 710
MspI CCGG 4 cut(s) 165, 201, 222, 651
MspR9I CCNGG 1 cut(s) 430
Mva1269I GAATGC 1 cut(s) 409
MvaI CCWGG 1 cut(s) 430
MvnI CGCG 3 cut(s) 155, 704, 710
MwoI GCNNNNNNNGC 8 cut(s) 152, 206, 215, 224, 347, 578, 587, 710
NaeI GCCGGC 1 cut(s) 202
NcoI CCATGG 1 cut(s) 234
NdeII GATC 3 cut(s) 10, 274, 621
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 6 cut(s) 17, 85, 238, 386, 445, 559
NlaIV GGNNCC 4 cut(s) 226, 419, 540, 697
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 5 cut(s) 29, 101, 360, 601, 641
NspI RCATGY 1 cut(s) 559
OliI CACNNNNGTG 1 cut(s) 608
PagI TCATGA 2 cut(s) 13, 441
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 1 cut(s) 409
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 77
PkrI GCNGC 6 cut(s) 154, 220, 259, 340, 712, 715
PleI GAGTC 1 cut(s) 456
PpsI GAGTC 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 428
PspEI GGTNACC 1 cut(s) 601
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 4 cut(s) 226, 419, 540, 697
PspPI GGNCC 1 cut(s) 392
PstI CTGCAG 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 49
RseI CAYNNNNRTG 4 cut(s) 18, 233, 387, 608
SacII CCGCGG 2 cut(s) 156, 711
SapI GCTCTTC 1 cut(s) 257
SatI GCNGC 6 cut(s) 153, 219, 258, 339, 711, 714
Sau3AI GATC 3 cut(s) 10, 274, 621
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 1 cut(s) 430
SduI GDGCHC 1 cut(s) 490
SetI ASST 7 cut(s) 108, 177, 182, 216, 348, 525, 664
SfaNI GCATC 2 cut(s) 36, 599
SfcI CTRYAG 1 cut(s) 491
Sfr303I CCGCGG 2 cut(s) 156, 711
SgrBI CCGCGG 2 cut(s) 156, 711
SmiMI CAYNNNNRTG 4 cut(s) 18, 233, 387, 608
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 1 cut(s) 449
SsiI CCGC 9 cut(s) 153, 155, 218, 246, 257, 581, 684, 708, 710
SspI AATATT 1 cut(s) 357
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 1 cut(s) 428
StyI CCWWGG 2 cut(s) 234, 576
TaaI ACNGT 2 cut(s) 128, 136
TaiI ACGT 3 cut(s) 108, 177, 525
TaqI TCGA 2 cut(s) 435, 465
TasI AATT 1 cut(s) 449
TauI GCSGC 4 cut(s) 155, 221, 260, 713
TscAI CASTG 3 cut(s) 94, 139, 600
TseFI GTSAC 5 cut(s) 29, 101, 360, 601, 641
TseI GCWGC 2 cut(s) 338, 713
Tsp45I GTSAC 5 cut(s) 29, 101, 360, 601, 641
TspDTI ATGAA 6 cut(s) 70, 324, 399, 430, 461, 507
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 3 cut(s) 94, 139, 600
XapI RAATTY 1 cut(s) 449
XceI RCATGY 1 cut(s) 559
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 1 cut(s) 458
ZraI GACGTC 2 cut(s) 106, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.