Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0003204

Physical Location & Seq

Genomic Coordinates Reverse (-)

30212859 .. 30213770

Gene Structure 912 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g29880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 912 bp

ATGAACGACTTATTTTCGAGCTCGTTCAAGAAGTACACGGACCTGAAGCAGCAGGCCTACCTCGACGACGTGGAGGCCGGCAAGGAAAACGTTAACCTCGACAAGTTCTTCGAAGACATTGAGAATGTGAAGGAGGACATGAGGCAGGTCGAGAGGCTGTACAAGCAGCTGCAAGAATCCAATGAGGAGAGCAAGACTGTGCACAATGCTCAAACCATGAAGGAGCTCCGGACCCGAATGGACTTGGACGTCGAGCAGGTCCTCAAAAAGGTCAAAATCATCAAGGGGAAGCTCGAAGGATTAGAGCGTTCGAACGCAGCGCACAGGAACCTTCCCGGGTGTGGCCCTGGATCCTCTGCTGATCGAACGAGGACATCGGTGGTTAGTGGCTTGGGGAAGAAGCTAAAGGACATGATGGATGACTTTCAAGGGTTGAGAGCTAGGATGACAGCCGAGTACAAGGAAACAATAGAACGCAGGTATTTCACAATCACCGGAGAGAAGGCAAGCGAGGATACCATCGAAACTTTGATTTCGAGCGGAGAGAGTGAGAGTTTTCTGCAGAAGGCGATTCAGGAGCAGGGGAGAGGTCAGATTATGGACACCATATCCGAAATGCAAGAGAGACACGATGCTGTGAAGGAGATCGAGAAGAATTTGATCGAGCTGCATCAGATATTCATGGACATGGCCGCCCTGGTCGAAGCTCAGGGCCATCAGCTCAACGACATCGAGAGCCACGTCATGCACGCAAGTTCTTTCGTGAGACGAGGCACTGACAACCTTCAGGTAGCCAAAGAACAGCAGAAGAAATCAAGGAAGTGGACATGCATTGCAATCATTCTTGGAATAGTTCTCATCATTCTCCTCCTAATTCCAATTATATTACAAATTTTGCCTCATATGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000149 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0016050 GO:0016192 GO:0022406 GO:0022414 GO:0030133 GO:0030154 GO:0031201 GO:0031224 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032940 GO:0032989 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0044706 GO:0046903 GO:0048278 GO:0048284 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051704 GO:0060560 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0097708 GO:0098796 GO:0140056

KEGG Pathways

Metabolic & Signaling

ko04130 ko04721 map04130 map04721

Pfam Domains

Protein Families

SNARE Syntaxin

Protein Analysis

304

Amino Acids

34.7

Weight (kDa)

5.98

Isoelectric Point (pI)

46.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syntaxin	PF00804	32 - 191	8.8e-53	Syntaxin
SNARE	PF05739	240 - 291	1.8e-16	SNARE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11250 AT1G61290 AT1G61290 AT4G03330
fragaria_vesca	FvH4_3g04130 FvH4_6g44820
malus_domestica	MD05G1324600.v1.1 MD10G1302400.v1.1 MD17G1082900.v1.1
prunus_persica	Prupe.3G232300_v2.0.a1 Prupe.4G036000_v2.0.a1
pyrus_communis	pycom05g29880 pycom10g25490
rosa_chinensis	RchiOBHm_Chr2g0162001 RchiOBHm_Chr5g0006581
rosa_laevigata	RLG00000021314 RLG00000031401
rosa_multiflora	Rmu_co8449309.1_g000002 Rmu_co8484739.1_g000001 Rmu_sc0005741.1_g000004 Rmu_sc0008428.1_g000004 Rmu_sc0011154.1_g000007 Rmu_sc0038528.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069460 Rroxscaffold_2G00088410
rosa_rugosa	Rorug02G0493900 Rorug04G0422800
rosa_samantha	Rh2AG559800 Rh2BG573200 Rh2CG543400 Rh2DG583000 Rh5AG049900 Rh5BG049200 Rh5CG057600 Rh5DG048600
rosa_wichuraiana	Rw2G046330 Rw5G005000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	248
AatII	GACGTC	1 cut(s)	252
Acc36I	ACCTGC	3 cut(s)	136, 247, 468
AccBSI	CCGCTC	1 cut(s)	540
AccIII	TCCGGA	1 cut(s)	228
AciI	CCGC	2 cut(s)	540, 693
AclI	AACGTT	1 cut(s)	90
AclWI	GGATC	2 cut(s)	345, 358
AcoI	YGGCCR	1 cut(s)	690
AcsI	RAATTY	2 cut(s)	655, 891
AcuI	CTGAAG	2 cut(s)	65, 770
AcyI	GRCGYC	1 cut(s)	249
AfaI	GTAC	3 cut(s)	35, 161, 458
AfiI	CCNNNNNNNGG	2 cut(s)	268, 341
AgsI	TTSAA	2 cut(s)	28, 428
AjiI	CACGTC	2 cut(s)	70, 742
AjnI	CCWGG	2 cut(s)	346, 696
AluBI	AGCT	9 cut(s)	21, 169, 226, 292, 403, 440, 667, 707, 721
AluI	AGCT	9 cut(s)	21, 169, 226, 292, 403, 440, 667, 707, 721
Alw21I	GWGCWC	3 cut(s)	23, 204, 228
Alw26I	GTCTC	2 cut(s)	619, 760
Alw44I	GTGCAC	1 cut(s)	200
AlwI	GGATC	2 cut(s)	345, 358
Ama87I	CYCGRG	1 cut(s)	335
Aor13HI	TCCGGA	1 cut(s)	228
AoxI	GGCC	5 cut(s)	54, 75, 343, 690, 712
ApaLI	GTGCAC	1 cut(s)	200
ApeKI	GCWGC	5 cut(s)	49, 166, 169, 317, 667
ApoI	RAATTY	2 cut(s)	655, 891
ArsI	GACNNNNNNTTYG	2 cut(s)	92, 124
AspLEI	GCGC	1 cut(s)	322
AspS9I	GGNCC	5 cut(s)	40, 231, 259, 344, 712
AsuC2I	CCSGG	2 cut(s)	336, 337
AsuHPI	GGTGA	1 cut(s)	484
AsuII	TTCGAA	2 cut(s)	111, 311
AvaI	CYCGRG	1 cut(s)	335
AvaII	GGWCC	3 cut(s)	40, 231, 259
BaeGI	GKGCMC	1 cut(s)	204
BamHI	GGATCC	1 cut(s)	350
BanII	GRGCYC	2 cut(s)	23, 228
BbsI	GAAGAC	1 cut(s)	120
Bbv12I	GWGCWC	3 cut(s)	23, 204, 228
BbvI	GCAGC	5 cut(s)	61, 156, 178, 329, 654
BccI	CCATC	3 cut(s)	409, 527, 723
BciT130I	CCWGG	2 cut(s)	348, 698
BciVI	GTATCC	1 cut(s)	508
BcnI	CCSGG	2 cut(s)	336, 337
BcoDI	GTCTC	2 cut(s)	619, 760
BfaI	CTAG	1 cut(s)	441
BfmI	CTRYAG	1 cut(s)	560
BfuAI	ACCTGC	3 cut(s)	136, 247, 468
BfuI	GTATCC	1 cut(s)	508
BisI	GCNGC	6 cut(s)	50, 167, 170, 318, 668, 693
BlsI	GCNGC	6 cut(s)	51, 168, 171, 319, 669, 694
Bme1390I	CCNGG	4 cut(s)	336, 337, 348, 698
Bme18I	GGWCC	3 cut(s)	40, 231, 259
BmeT110I	CYCGRG	1 cut(s)	335
BmgBI	CACGTC	2 cut(s)	70, 742
BmgT120I	GGNCC	5 cut(s)	40, 231, 259, 344, 712
BmiI	GGNNCC	3 cut(s)	233, 329, 352
BmrFI	CCNGG	4 cut(s)	336, 337, 348, 698
BmsI	GCATC	2 cut(s)	622, 679
BpiI	GAAGAC	1 cut(s)	120
Bpu10I	CCTNAGC	1 cut(s)	708
Bpu14I	TTCGAA	2 cut(s)	111, 311
BpuMI	CCSGG	2 cut(s)	336, 337
BsaHI	GRCGYC	1 cut(s)	249
BsaJI	CCNNGG	3 cut(s)	335, 346, 696
BsaWI	WCCGGW	2 cut(s)	228, 494
Bsc4I	CCNNNNNNNGG	2 cut(s)	268, 341
Bse118I	RCCGGY	1 cut(s)	77
Bse3DI	GCAATG	1 cut(s)	831
BseAI	TCCGGA	1 cut(s)	228
BseBI	CCWGG	2 cut(s)	348, 698
BseDI	CCNNGG	3 cut(s)	335, 346, 696
BseGI	GGATG	2 cut(s)	424, 450
BseLI	CCNNNNNNNGG	2 cut(s)	268, 341
BseMI	GCAATG	1 cut(s)	831
BseMII	CTCAG	1 cut(s)	722
BseRI	GAGGAG	2 cut(s)	200, 857
BseSI	GKGCMC	1 cut(s)	204
BseXI	GCAGC	5 cut(s)	61, 156, 178, 329, 654
BshFI	GGCC	5 cut(s)	56, 77, 345, 692, 714
BsiHKAI	GWGCWC	3 cut(s)	23, 204, 228
BsiHKCI	CYCGRG	1 cut(s)	335
BsiSI	CCGG	4 cut(s)	78, 229, 336, 495
BslI	CCNNNNNNNGG	2 cut(s)	268, 341
BsmAI	GTCTC	2 cut(s)	619, 760
BsmBI	CGTCTC	1 cut(s)	760
BsnI	GGCC	5 cut(s)	56, 77, 345, 692, 714
BsoBI	CYCGRG	1 cut(s)	335
Bsp119I	TTCGAA	2 cut(s)	111, 311
Bsp1286I	GDGCHC	3 cut(s)	23, 204, 228
Bsp13I	TCCGGA	1 cut(s)	228
Bsp1407I	TGTACA	1 cut(s)	159
Bsp143I	GATC	4 cut(s)	350, 361, 645, 660
BspACI	CCGC	2 cut(s)	540, 693
BspANI	GGCC	5 cut(s)	56, 77, 345, 692, 714
BspCNI	CTCAG	1 cut(s)	721
BspEI	TCCGGA	1 cut(s)	228
BspLI	GGNNCC	3 cut(s)	233, 329, 352
BspMAI	CTGCAG	1 cut(s)	564
BspMI	ACCTGC	3 cut(s)	136, 247, 468
BspPI	GGATC	2 cut(s)	345, 358
BspT104I	TTCGAA	2 cut(s)	111, 311
BsrBI	CCGCTC	1 cut(s)	540
BsrDI	GCAATG	1 cut(s)	831
BsrFI	RCCGGY	1 cut(s)	77
BsrGI	TGTACA	1 cut(s)	159
BssAI	RCCGGY	1 cut(s)	77
BssECI	CCNNGG	3 cut(s)	335, 346, 696
BssMI	GATC	4 cut(s)	350, 361, 645, 660
BssNI	GRCGYC	1 cut(s)	249
Bst2UI	CCWGG	2 cut(s)	348, 698
Bst4CI	ACNGT	1 cut(s)	199
BstACI	GRCGYC	1 cut(s)	249
BstAUI	TGTACA	1 cut(s)	159
BstBI	TTCGAA	2 cut(s)	111, 311
BstC8I	GCNNGC	4 cut(s)	54, 79, 508, 750
BstDEI	CTNAG	1 cut(s)	708
BstENI	CCTNNNNNAGG	1 cut(s)	266
BstF5I	GGATG	2 cut(s)	424, 450
BstHHI	GCGC	1 cut(s)	322
BstKTI	GATC	4 cut(s)	353, 364, 648, 663
BstMAI	GTCTC	2 cut(s)	619, 760
BstMBI	GATC	4 cut(s)	350, 361, 645, 660
BstMWI	GCNNNNNNNGC	1 cut(s)	163
BstNI	CCWGG	2 cut(s)	348, 698
BstNSI	RCATGY	1 cut(s)	831
BstSCI	CCNGG	4 cut(s)	334, 335, 346, 696
BstSFI	CTRYAG	1 cut(s)	560
BstSLI	GKGCMC	1 cut(s)	204
BstV1I	GCAGC	5 cut(s)	61, 156, 178, 329, 654
BstV2I	GAAGAC	1 cut(s)	120
BstX2I	RGATCY	1 cut(s)	350
BstYI	RGATCY	1 cut(s)	350
BsuI	GTATCC	1 cut(s)	508
BsuRI	GGCC	5 cut(s)	56, 77, 345, 692, 714
BtrI	CACGTC	2 cut(s)	70, 742
BtsCI	GGATG	2 cut(s)	424, 450
BtsIMutI	CAGTG	1 cut(s)	774
BveI	ACCTGC	3 cut(s)	136, 247, 468
Cac8I	GCNNGC	4 cut(s)	54, 79, 508, 750
CfoI	GCGC	1 cut(s)	322
Cfr10I	RCCGGY	1 cut(s)	77
Cfr13I	GGNCC	5 cut(s)	40, 231, 259, 344, 712
Cfr9I	CCCGGG	1 cut(s)	335
Csp6I	GTAC	3 cut(s)	34, 160, 457
CviAII	CATG	7 cut(s)	139, 217, 412, 682, 688, 745, 828
CviQI	GTAC	3 cut(s)	34, 160, 457
DdeI	CTNAG	1 cut(s)	708
DpnI	GATC	4 cut(s)	352, 363, 647, 662
DpnII	GATC	4 cut(s)	350, 361, 645, 660
DrdI	GACNNNNNNGTC	1 cut(s)	248
DseDI	GACNNNNNNGTC	1 cut(s)	248
EaeI	YGGCCR	1 cut(s)	690
Ecl136II	GAGCTC	2 cut(s)	21, 226
Eco147I	AGGCCT	1 cut(s)	56
Eco24I	GRGCYC	2 cut(s)	23, 228
Eco47I	GGWCC	3 cut(s)	40, 231, 259
Eco53kI	GAGCTC	2 cut(s)	21, 226
Eco57I	CTGAAG	2 cut(s)	65, 770
Eco88I	CYCGRG	1 cut(s)	335
EcoICRI	GAGCTC	2 cut(s)	21, 226
EcoNI	CCTNNNNNAGG	1 cut(s)	266
EcoO109I	RGGNCCY	1 cut(s)	259
EcoRII	CCWGG	2 cut(s)	346, 696
EcoT22I	ATGCAT	1 cut(s)	833
EcoT38I	GRGCYC	2 cut(s)	23, 228
Esp3I	CGTCTC	1 cut(s)	760
FaeI	CATG	7 cut(s)	142, 220, 415, 685, 691, 748, 831
FatI	CATG	7 cut(s)	138, 216, 411, 681, 687, 744, 827
FauNDI	CATATG	1 cut(s)	903
Fnu4HI	GCNGC	6 cut(s)	50, 167, 170, 318, 668, 693
FokI	GGATG	2 cut(s)	431, 457
FriOI	GRGCYC	2 cut(s)	23, 228
Fsp4HI	GCNGC	6 cut(s)	50, 167, 170, 318, 668, 693
FspBI	CTAG	1 cut(s)	441
GlaI	GCGC	1 cut(s)	321
GluI	GCNGC	6 cut(s)	50, 167, 170, 318, 668, 693
HaeIII	GGCC	5 cut(s)	56, 77, 345, 692, 714
HapII	CCGG	4 cut(s)	78, 229, 336, 495
HhaI	GCGC	1 cut(s)	322
Hin1I	GRCGYC	1 cut(s)	249
Hin1II	CATG	7 cut(s)	142, 220, 415, 685, 691, 748, 831
Hin6I	GCGC	1 cut(s)	320
HinP1I	GCGC	1 cut(s)	320
HincII	GTYRAC	1 cut(s)	94
HindII	GTYRAC	1 cut(s)	94
HinfI	GANTC	2 cut(s)	176, 571
HpaI	GTTAAC	1 cut(s)	94
HpaII	CCGG	4 cut(s)	78, 229, 336, 495
HphI	GGTGA	1 cut(s)	484
Hpy166II	GTNNAC	4 cut(s)	36, 94, 202, 825
Hpy188I	TCNGA	3 cut(s)	594, 613, 675
Hpy188III	TCNNGA	7 cut(s)	28, 151, 229, 575, 649, 733, 763
Hpy8I	GTNNAC	4 cut(s)	36, 94, 202, 825
Hpy99I	CGWCG	3 cut(s)	68, 71, 254
HpyAV	CCTTC	8 cut(s)	124, 214, 290, 341, 496, 559, 634, 794
HpyCH4III	ACNGT	1 cut(s)	199
HpyCH4IV	ACGT	4 cut(s)	69, 90, 249, 741
HpyCH4V	TGCA	8 cut(s)	172, 202, 562, 619, 670, 748, 831, 836
HpyF10VI	GCNNNNNNNGC	1 cut(s)	163
HpyF3I	CTNAG	1 cut(s)	708
HpySE526I	ACGT	4 cut(s)	69, 90, 249, 741
Hsp92I	GRCGYC	1 cut(s)	249
Hsp92II	CATG	7 cut(s)	142, 220, 415, 685, 691, 748, 831
HspAI	GCGC	1 cut(s)	320
Kpn2I	TCCGGA	1 cut(s)	228
KroI	GCCGGC	1 cut(s)	77
KroNI	GCCGGC	1 cut(s)	79
KspAI	GTTAAC	1 cut(s)	94
Kzo9I	GATC	4 cut(s)	350, 361, 645, 660
LmnI	GCTCC	3 cut(s)	223, 231, 577
Lsp1109I	GCAGC	5 cut(s)	61, 156, 178, 329, 654
LweI	GCATC	2 cut(s)	622, 679
MaeI	CTAG	1 cut(s)	441
MaeII	ACGT	4 cut(s)	69, 90, 249, 741
MalI	GATC	4 cut(s)	352, 363, 647, 662
MbiI	CCGCTC	1 cut(s)	540
MboI	GATC	4 cut(s)	350, 361, 645, 660
MboII	GAAGA	5 cut(s)	100, 125, 409, 664, 820
MflI	RGATCY	1 cut(s)	350
MhlI	GDGCHC	3 cut(s)	23, 204, 228
MluCI	AATT	4 cut(s)	655, 873, 879, 891
Mph1103I	ATGCAT	1 cut(s)	833
MroI	TCCGGA	1 cut(s)	228
MroNI	GCCGGC	1 cut(s)	77
MseI	TTAA	1 cut(s)	93
MslI	CAYNNNNRTG	1 cut(s)	686
MspA1I	CMGCKG	1 cut(s)	169
MspI	CCGG	4 cut(s)	78, 229, 336, 495
MspR9I	CCNGG	4 cut(s)	336, 337, 348, 698
MvaI	CCWGG	2 cut(s)	348, 698
MwoI	GCNNNNNNNGC	1 cut(s)	163
NaeI	GCCGGC	1 cut(s)	79
NciI	CCSGG	2 cut(s)	336, 337
NdeI	CATATG	1 cut(s)	903
NdeII	GATC	4 cut(s)	350, 361, 645, 660
NgoMIV	GCCGGC	1 cut(s)	77
NlaIII	CATG	7 cut(s)	142, 220, 415, 685, 691, 748, 831
NlaIV	GGNNCC	3 cut(s)	233, 329, 352
NmeAIII	GCCGAG	1 cut(s)	478
NsiI	ATGCAT	1 cut(s)	833
NspI	RCATGY	1 cut(s)	831
NspV	TTCGAA	2 cut(s)	111, 311
PceI	AGGCCT	1 cut(s)	56
PcsI	WCGNNNNNNNCGW	2 cut(s)	96, 738
PdiI	GCCGGC	1 cut(s)	79
PfeI	GAWTC	2 cut(s)	176, 571
PkrI	GCNGC	6 cut(s)	51, 168, 171, 319, 669, 694
PpuMI	RGGWCCY	1 cut(s)	259
Psp124BI	GAGCTC	2 cut(s)	23, 228
Psp1406I	AACGTT	1 cut(s)	90
Psp5II	RGGWCCY	1 cut(s)	259
Psp6I	CCWGG	2 cut(s)	346, 696
PspGI	CCWGG	2 cut(s)	346, 696
PspN4I	GGNNCC	3 cut(s)	233, 329, 352
PspPI	GGNCC	5 cut(s)	40, 231, 259, 344, 712
PspPPI	RGGWCCY	1 cut(s)	259
PstI	CTGCAG	1 cut(s)	564
PsuI	RGATCY	1 cut(s)	350
PvuII	CAGCTG	1 cut(s)	169
RsaI	GTAC	3 cut(s)	35, 161, 458
RsaNI	GTAC	3 cut(s)	34, 160, 457
RseI	CAYNNNNRTG	1 cut(s)	686
SacI	GAGCTC	2 cut(s)	23, 228
SaqAI	TTAA	1 cut(s)	93
SatI	GCNGC	6 cut(s)	50, 167, 170, 318, 668, 693
Sau3AI	GATC	4 cut(s)	350, 361, 645, 660
Sau96I	GGNCC	5 cut(s)	40, 231, 259, 344, 712
ScrFI	CCNGG	4 cut(s)	336, 337, 348, 698
SduI	GDGCHC	3 cut(s)	23, 204, 228
SfaNI	GCATC	2 cut(s)	622, 679
SfcI	CTRYAG	1 cut(s)	560
SfuI	TTCGAA	2 cut(s)	111, 311
SinI	GGWCC	3 cut(s)	40, 231, 259
SmaI	CCCGGG	1 cut(s)	337
SmiMI	CAYNNNNRTG	1 cut(s)	686
Sse9I	AATT	4 cut(s)	655, 873, 879, 891
SseBI	AGGCCT	1 cut(s)	56
SsiI	CCGC	2 cut(s)	540, 693
SspMI	CTAG	1 cut(s)	441
SstI	GAGCTC	2 cut(s)	23, 228
StuI	AGGCCT	1 cut(s)	56
StyD4I	CCNGG	4 cut(s)	334, 335, 346, 696
TaaI	ACNGT	1 cut(s)	199
TaiI	ACGT	4 cut(s)	72, 93, 252, 744
TasI	AATT	4 cut(s)	655, 873, 879, 891
TatI	WGTACW	3 cut(s)	33, 159, 456
TauI	GCSGC	1 cut(s)	695
TfiI	GAWTC	2 cut(s)	176, 571
Tru1I	TTAA	1 cut(s)	93
Tru9I	TTAA	1 cut(s)	93
TscAI	CASTG	1 cut(s)	781
TseI	GCWGC	5 cut(s)	49, 166, 169, 317, 667
TspDTI	ATGAA	3 cut(s)	17, 233, 670
TspGWI	ACGGA	1 cut(s)	53
TspMI	CCCGGG	1 cut(s)	335
TspRI	CASTG	1 cut(s)	781
VneI	GTGCAC	1 cut(s)	200
VpaK11BI	GGWCC	3 cut(s)	40, 231, 259
XagI	CCTNNNNNAGG	1 cut(s)	266
XapI	RAATTY	2 cut(s)	655, 891
XceI	RCATGY	1 cut(s)	831
XmaI	CCCGGG	1 cut(s)	335
XspI	CTAG	1 cut(s)	441
ZraI	GACGTC	1 cut(s)	250
Zsp2I	ATGCAT	1 cut(s)	833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.