Rh5AG050000

Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006238
Physical Location & Seq
Reverse (-)
4244109 .. 4244738
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG050000.1

Sequence Viewer

Length: 630 bp
ATGGAGGTCAAGCTATGGAATGACAAGCGTGAAAGAGAAATGTATGAAAATTTCTCTGAGTTATACGCCATTATAAAGGCCACTGAGAAGCTTGAAAGGGCATACATTCGCGACGTGATCTCTTCATCAGCGTATGAGACTGAATGCCAGAAACTCATTGCTCACTTTAAGACTTTGTCTTCCACGCTTAAAGACACCATCCCAAGCATTGAGCGCTTTGCAGATACGTATAAGATGGACTGCCCAGCAGCTATAAACCGCCTTGTGACCTCTGGGGTTCCTGCTACAGTGGAGCACAGGGCTGCTGCAGCTTCTTCCATGACTTCATCGGCCGCTGCTGTGGCAGAGTGCACACAGAATTTCATTACGGCAATGGACTCATTGAAGTTGAATATGATTGCAGTTGATCAGGTGCACCCCCTACTCTCGGACCTCGCAACATCTCTCAATAAGCTGACCTTTCTGCCACCGGATTTCTTGGGGAAGGTGAAGTTGAAGGAATGGATTGCAAGGCTGGCAAAGATGGGCGCAGCAGATGAGTTGACAGAGCAACAGGCCAGGCAGCTTCACTTTGATCTCGAATCATCCTACAACTCATTTATGGCAGCTTTACCCAATGCTGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
VPS28

Protein Analysis

209

Amino Acids

23.29

Weight (kDa)

5.63

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS28 PF03997 18 - 204 9.1e-72 VPS28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 74
AccII CGCG 1 cut(s) 111
AciI CCGC 2 cut(s) 259, 333
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 2 cut(s) 49, 358
AfeI AGCGCT 1 cut(s) 215
AfiI CCNNNNNNNGG 2 cut(s) 427, 620
AgsI TTSAA 4 cut(s) 95, 385, 391, 496
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 1 cut(s) 557
AluBI AGCT 7 cut(s) 13, 91, 251, 311, 454, 565, 608
AluI AGCT 7 cut(s) 13, 91, 251, 311, 454, 565, 608
Alw21I GWGCWC 3 cut(s) 297, 353, 417
Alw26I GTCTC 1 cut(s) 131
Alw44I GTGCAC 2 cut(s) 349, 413
Aor51HI AGCGCT 1 cut(s) 215
AoxI GGCC 3 cut(s) 78, 330, 555
ApaLI GTGCAC 2 cut(s) 349, 413
ApeKI GCWGC 8 cut(s) 248, 302, 305, 308, 335, 530, 562, 605
ApoI RAATTY 2 cut(s) 49, 358
AspLEI GCGC 2 cut(s) 216, 530
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 1 cut(s) 499
AvaII GGWCC 1 cut(s) 430
BaeGI GKGCMC 2 cut(s) 353, 417
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 3 cut(s) 297, 353, 417
BbvI GCAGC 8 cut(s) 260, 289, 292, 320, 322, 542, 574, 617
BccI CCATC 3 cut(s) 206, 229, 517
BceAI ACGGC 1 cut(s) 384
BciT130I CCWGG 1 cut(s) 559
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 131
BfmI CTRYAG 2 cut(s) 285, 306
BfoI RGCGCY 1 cut(s) 217
BisI GCNGC 9 cut(s) 249, 303, 306, 309, 333, 336, 531, 563, 606
BlsI GCNGC 9 cut(s) 250, 304, 307, 310, 334, 337, 532, 564, 607
Bme1390I CCNGG 1 cut(s) 559
Bme18I GGWCC 1 cut(s) 430
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 559
BpiI GAAGAC 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 469
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 620
Bse3DI GCAATG 2 cut(s) 156, 378
BseBI CCWGG 1 cut(s) 559
BseGI GGATG 2 cut(s) 198, 584
BseLI CCNNNNNNNGG 2 cut(s) 427, 620
BseMI GCAATG 2 cut(s) 156, 378
BseMII CTCAG 2 cut(s) 48, 75
BseSI GKGCMC 2 cut(s) 353, 417
BseX3I CGGCCG 1 cut(s) 330
BseXI GCAGC 8 cut(s) 260, 289, 292, 320, 322, 542, 574, 617
BseYI CCCAGC 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 111
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 3 cut(s) 80, 332, 557
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 3 cut(s) 297, 353, 417
BsiSI CCGG 1 cut(s) 470
BslI CCNNNNNNNGG 2 cut(s) 427, 620
BsmAI GTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 80, 332, 557
Bsp1286I GDGCHC 3 cut(s) 297, 353, 417
Bsp143I GATC 3 cut(s) 117, 406, 574
Bsp68I TCGCGA 1 cut(s) 111
BspACI CCGC 2 cut(s) 259, 333
BspANI GGCC 3 cut(s) 80, 332, 557
BspCNI CTCAG 2 cut(s) 49, 76
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 156, 378
BssMI GATC 3 cut(s) 117, 406, 574
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 127
BstBAI YACGTR 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 2 cut(s) 57, 84
BstF5I GGATG 2 cut(s) 198, 584
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 2 cut(s) 216, 530
BstKTI GATC 3 cut(s) 120, 409, 577
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 3 cut(s) 117, 406, 574
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 4 cut(s) 213, 308, 341, 515
BstNI CCWGG 1 cut(s) 559
BstSCI CCNGG 1 cut(s) 557
BstSFI CTRYAG 2 cut(s) 285, 306
BstSLI GKGCMC 2 cut(s) 353, 417
BstSNI TACGTA 1 cut(s) 228
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 8 cut(s) 260, 289, 292, 320, 322, 542, 574, 617
BstV2I GAAGAC 1 cut(s) 171
BstZI CGGCCG 1 cut(s) 330
BsuRI GGCC 3 cut(s) 80, 332, 557
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 2 cut(s) 198, 584
BtsIMutI CAGTG 2 cut(s) 81, 294
BtuMI TCGCGA 1 cut(s) 111
Cac8I GCNNGC 1 cut(s) 516
CfoI GCGC 2 cut(s) 216, 530
Cfr13I GGNCC 1 cut(s) 430
CviAII CATG 1 cut(s) 319
DdeI CTNAG 2 cut(s) 57, 84
DpnI GATC 3 cut(s) 119, 408, 576
DpnII GATC 3 cut(s) 117, 406, 574
EaeI YGGCCR 1 cut(s) 330
EagI CGGCCG 1 cut(s) 330
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
EclXI CGGCCG 1 cut(s) 330
Eco105I TACGTA 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 430
Eco47III AGCGCT 1 cut(s) 215
Eco52I CGGCCG 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 557
FaeI CATG 1 cut(s) 322
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 1 cut(s) 318
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 9 cut(s) 249, 303, 306, 309, 333, 336, 531, 563, 606
FokI GGATG 2 cut(s) 185, 571
Fsp4HI GCNGC 9 cut(s) 249, 303, 306, 309, 333, 336, 531, 563, 606
GlaI GCGC 2 cut(s) 215, 529
GluI GCNGC 9 cut(s) 249, 303, 306, 309, 333, 336, 531, 563, 606
GsaI CCCAGC 1 cut(s) 248
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 3 cut(s) 80, 332, 557
HapII CCGG 1 cut(s) 470
HhaI GCGC 2 cut(s) 216, 530
Hin1II CATG 1 cut(s) 322
Hin6I GCGC 2 cut(s) 214, 528
HinP1I GCGC 2 cut(s) 214, 528
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 377, 581
HpaII CCGG 1 cut(s) 470
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 3 cut(s) 351, 415, 543
Hpy188I TCNGA 2 cut(s) 58, 430
Hpy188III TCNNGA 2 cut(s) 110, 578
Hpy8I GTNNAC 3 cut(s) 351, 415, 543
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 2 cut(s) 478, 490
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 114, 227
HpyCH4V TGCA 6 cut(s) 221, 308, 351, 401, 415, 509
HpyF10VI GCNNNNNNNGC 4 cut(s) 213, 308, 341, 515
HpyF3I CTNAG 2 cut(s) 57, 84
HpySE526I ACGT 2 cut(s) 114, 227
Hsp92II CATG 1 cut(s) 322
HspAI GCGC 2 cut(s) 214, 528
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 3 cut(s) 117, 406, 574
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 8 cut(s) 260, 289, 292, 320, 322, 542, 574, 617
MaeII ACGT 2 cut(s) 114, 227
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 119, 408, 576
MboI GATC 3 cut(s) 117, 406, 574
MboII GAAGA 3 cut(s) 114, 171, 306
MhlI GDGCHC 3 cut(s) 297, 353, 417
MluCI AATT 3 cut(s) 49, 358, 625
MlyI GAGTC 1 cut(s) 371
MnlI CCTC 2 cut(s) 280, 443
MseI TTAA 2 cut(s) 168, 189
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 1 cut(s) 559
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 1 cut(s) 559
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 4 cut(s) 213, 308, 341, 515
NdeII GATC 3 cut(s) 117, 406, 574
NlaIII CATG 1 cut(s) 322
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 265
NruI TCGCGA 1 cut(s) 111
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 581
PflFI GACNNNGTC 1 cut(s) 175
PkrI GCNGC 9 cut(s) 250, 304, 307, 310, 334, 337, 532, 564, 607
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
Ppu21I YACGTR 1 cut(s) 228
PsiI TTATAA 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 557
PspFI CCCAGC 1 cut(s) 244
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 430
PstI CTGCAG 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 175
RruI TCGCGA 1 cut(s) 111
SaqAI TTAA 2 cut(s) 168, 189
SatI GCNGC 9 cut(s) 249, 303, 306, 309, 333, 336, 531, 563, 606
Sau3AI GATC 3 cut(s) 117, 406, 574
Sau96I GGNCC 1 cut(s) 430
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 1 cut(s) 559
SduI GDGCHC 3 cut(s) 297, 353, 417
SfcI CTRYAG 2 cut(s) 285, 306
SinI GGWCC 1 cut(s) 430
SnaBI TACGTA 1 cut(s) 228
Sse9I AATT 3 cut(s) 49, 358, 625
SsiI CCGC 2 cut(s) 259, 333
StyD4I CCNGG 1 cut(s) 557
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 2 cut(s) 117, 230
TaqI TCGA 1 cut(s) 579
TasI AATT 3 cut(s) 49, 358, 625
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 2 cut(s) 168, 189
Tru9I TTAA 2 cut(s) 168, 189
TscAI CASTG 2 cut(s) 88, 294
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 8 cut(s) 248, 302, 305, 308, 335, 530, 562, 605
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 4 cut(s) 60, 114, 315, 352
TspRI CASTG 2 cut(s) 88, 294
Tth111I GACNNNGTC 1 cut(s) 175
VneI GTGCAC 2 cut(s) 349, 413
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 2 cut(s) 49, 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.