Prupe.4G204200_v2.0.a1

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0018273
Physical Location & Seq
Forward (+)
12719798 .. 12722741
2944 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G204200.1

Sequence Viewer

Length: 1374 bp
ATGCAGATCTTCGTGAAAACCCTCACAGGCAAGACCATCACCCTCGAGGTTGAAAGCTCTGACACCATTGACAATGTCAAAGCCAAGATTCAGGACAAGGAAGGCATTCCCCCAGACCAGCAGAGGCTGATCTTTGCTGGCAAGCAGCTTGAGGATGGCCGCACCCTCGCCGATTACAACATCCAGAAGGAGTCCACCCTCCACTTGGTGCTTCGTCTCCGTGGTGGTATGCAAATCTTTGTCAAGACCCTTACCGGGAAGACAATCACCTTGGAAGTTGAAAGCTCGGATACTATTGACAATGTCAAGGCAAAGATCCAGGACAAGGAGGGAATTCCCCCAGACCAGCAGAGGCTCATCTTTGCTGGAAAGCAGCTTGAGGATGGTCGCACCCTTGCCGACTACAACATCCAGAAGGAGTCCACCCTTCACTTGGTGCTCCGACTACGCGGTGGTATGCAAATCTTTGTTAAGACCCTTACTGGGAAGACAATCACCTTGGAAGTTGAAAGCTCGGATACCATTGACAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATTCCCCCAGACCAGCAGAGGCTCATCTTTGCCGGTAAGCAGCTTGAGGATGGACGCACCCTTGCTGACTATAACATCCAAAAAGAATCGACTCTGCATTTGGTGCTCCGGCTGCGTGGTGGTATGCAGATATTTGTGAAAACTCTGACCGGGAAAACCATTACTCTGGAAGTGGAAAGCTCTGATACTATTGATAATGTTAAGGCCAAAATCCAGGACAAGGAGGGTATTCCCCCAGACCAGCAGAGGTTGATCTTTGCAGGCAAGCAGCTCGAGGATGGAAGGACCCTTGCTGACTATAACATTCAAAAGGAGTCCACTCTGCACTTGGTGCTCCGGCTGCGTGGTGGTATGCAGATATTTGTGAAAACTCTGACCGGGAAAACCATTACTCTGGAAGTGGAAAGCTCTGATACTATTGATAATGTTAAGGCCAAAATCCAGGACAAGGAGGGTATTCCCCCAGACCAGCAGAGGTTGATCTTTGCAGGCAAGCAGCTCGAGGATGGAAGGACCCTTGCTGACTACAACATTCAAAAGGAGTCCACTCTGCACTTGGTGCTTCGACTGCGTGGTGGGATGCAGATCTTTGTGAAGACTCTGACTGGCAAGACCATAACGTTGGAGGTGGAGAGTTCGGATACAATTGATAATGTGAAGGCCAAGATTCAGGACAAGGAGGGCATTCCTCCAGACCAGCAGAGGCTCATCTTTGCCGGTAAGCAGCTCGAGGATGGGAGGACTCTTGCAGATTACAACATTCAGAAGGAGTCTACCCTTCACCTTGTCCTTCGTCTCCGAGGTGGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

458

Amino Acids

51.17

Weight (kDa)

7.05

Isoelectric Point (pI)

29.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11970
prunus_persica Prupe.4G204200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359151
rosa_multiflora Rmu_sc0010589.1_g000003 Rmu_ssc0000135.1_g000004
rosa_rugosa Rorug03G0165600
rosa_samantha Rh1AG281700 Rh1CG266100 Rh1DG277200
rosa_wichuraiana Rw1G025040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 44
AccI GTMKAC 1 cut(s) 1334
AccII CGCG 1 cut(s) 450
AciI CCGC 2 cut(s) 160, 450
AclI AACGTT 1 cut(s) 1181
AclWI GGATC 2 cut(s) 310, 538
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 333
AdeI CACNNNGTG 4 cut(s) 208, 436, 892, 1120
AfiI CCNNNNNNNGG 8 cut(s) 205, 255, 325, 433, 483, 553, 781, 1009
AgsI TTSAA 5 cut(s) 53, 281, 509, 869, 1097
AjnI CCWGG 4 cut(s) 318, 546, 774, 1002
Alw21I GWGCWC 3 cut(s) 441, 669, 897
Alw26I GTCTC 2 cut(s) 221, 1361
AlwI GGATC 2 cut(s) 310, 538
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 4 cut(s) 44, 833, 1061, 1289
AoxI GGCC 4 cut(s) 157, 765, 993, 1221
ApeKI GCWGC 8 cut(s) 145, 373, 601, 673, 829, 901, 1057, 1285
ApoI RAATTY 1 cut(s) 333
ArsI GACNNNNNNTTYG 2 cut(s) 643, 675
Asp700I GAANNNNTTC 1 cut(s) 105
AspS9I GGNCC 2 cut(s) 846, 1074
AsuC2I CCSGG 3 cut(s) 256, 712, 940
AsuHPI GGTGA 4 cut(s) 31, 259, 487, 1334
AvaI CYCGRG 4 cut(s) 44, 833, 1061, 1289
AvaII GGWCC 2 cut(s) 846, 1074
BbsI GAAGAC 3 cut(s) 266, 494, 1163
Bbv12I GWGCWC 3 cut(s) 441, 669, 897
BbvI GCAGC 8 cut(s) 157, 385, 613, 660, 841, 888, 1069, 1297
BccI CCATC 7 cut(s) 44, 149, 377, 605, 833, 1061, 1289
BcgI CGANNNNNNTGC 4 cut(s) 814, 848, 1042, 1076
BciT130I CCWGG 4 cut(s) 320, 548, 776, 1004
BciVI GTATCC 3 cut(s) 283, 511, 1195
BcnI CCSGG 3 cut(s) 256, 712, 940
BcoDI GTCTC 2 cut(s) 221, 1361
BfuI GTATCC 3 cut(s) 283, 511, 1195
BglII AGATCT 2 cut(s) 6, 1146
BisI GCNGC 9 cut(s) 146, 160, 374, 602, 674, 830, 902, 1058, 1286
BlsI GCNGC 9 cut(s) 147, 161, 375, 603, 675, 831, 903, 1059, 1287
Bme1390I CCNGG 7 cut(s) 256, 320, 548, 712, 776, 940, 1004
Bme18I GGWCC 2 cut(s) 846, 1074
BmeT110I CYCGRG 4 cut(s) 44, 833, 1061, 1289
BmgT120I GGNCC 2 cut(s) 846, 1074
BmiI GGNNCC 2 cut(s) 848, 1076
BmrFI CCNGG 7 cut(s) 256, 320, 548, 712, 776, 940, 1004
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 1 cut(s) 1131
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 3 cut(s) 266, 494, 1163
BplI GAGNNNNNCTC 1 cut(s) 1353
BpmI CTGGAG 1 cut(s) 1236
BpuEI CTTGAG 3 cut(s) 170, 398, 626
BpuMI CCSGG 3 cut(s) 256, 712, 940
BsaBI GATNNNNATC 1 cut(s) 1145
BsaJI CCNNGG 4 cut(s) 220, 270, 498, 1360
Bsc4I CCNNNNNNNGG 8 cut(s) 205, 255, 325, 433, 483, 553, 781, 1009
Bse118I RCCGGY 2 cut(s) 593, 1277
Bse1I ACTGG 2 cut(s) 487, 1171
Bse8I GATNNNNATC 1 cut(s) 1145
BseBI CCWGG 4 cut(s) 320, 548, 776, 1004
BseDI CCNNGG 4 cut(s) 220, 270, 498, 1360
BseJI GATNNNNATC 1 cut(s) 1145
BseLI CCNNNNNNNGG 8 cut(s) 205, 255, 325, 433, 483, 553, 781, 1009
BseNI ACTGG 2 cut(s) 487, 1171
BseXI GCAGC 8 cut(s) 157, 385, 613, 660, 841, 888, 1069, 1297
BsgI GTGCAG 2 cut(s) 869, 1097
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 4 cut(s) 159, 767, 995, 1223
BsiHKAI GWGCWC 3 cut(s) 441, 669, 897
BsiHKCI CYCGRG 4 cut(s) 44, 833, 1061, 1289
BsiSI CCGG 7 cut(s) 255, 594, 670, 711, 898, 939, 1278
BslI CCNNNNNNNGG 8 cut(s) 205, 255, 325, 433, 483, 553, 781, 1009
BsmAI GTCTC 2 cut(s) 221, 1361
BsmBI CGTCTC 2 cut(s) 221, 1361
BsmI GAATGC 3 cut(s) 105, 561, 1245
BsnI GGCC 4 cut(s) 159, 767, 995, 1223
BsoBI CYCGRG 4 cut(s) 44, 833, 1061, 1289
Bsp1286I GDGCHC 3 cut(s) 441, 669, 897
Bsp143I GATC 7 cut(s) 6, 129, 315, 543, 813, 1041, 1146
BspACI CCGC 2 cut(s) 160, 450
BspANI GGCC 4 cut(s) 159, 767, 995, 1223
BspFNI CGCG 1 cut(s) 450
BspLI GGNNCC 2 cut(s) 848, 1076
BspPI GGATC 2 cut(s) 310, 538
BsrFI RCCGGY 2 cut(s) 593, 1277
BsrI ACTGG 2 cut(s) 487, 1171
BssAI RCCGGY 2 cut(s) 593, 1277
BssECI CCNNGG 4 cut(s) 220, 270, 498, 1360
BssMI GATC 7 cut(s) 6, 129, 315, 543, 813, 1041, 1146
BssT1I CCWWGG 2 cut(s) 270, 498
Bst2UI CCWGG 4 cut(s) 320, 548, 776, 1004
BstAPI GCANNNNNTGC 3 cut(s) 664, 892, 1120
BstC8I GCNNGC 6 cut(s) 139, 143, 823, 827, 1051, 1055
BstDSI CCRYGG 1 cut(s) 220
BstFNI CGCG 1 cut(s) 450
BstKTI GATC 7 cut(s) 9, 132, 318, 546, 816, 1044, 1149
BstMAI GTCTC 2 cut(s) 221, 1361
BstMBI GATC 7 cut(s) 6, 129, 315, 543, 813, 1041, 1146
BstMWI GCNNNNNNNGC 6 cut(s) 664, 673, 892, 901, 1120, 1129
BstNI CCWGG 4 cut(s) 320, 548, 776, 1004
BstSCI CCNGG 7 cut(s) 254, 318, 546, 710, 774, 938, 1002
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 8 cut(s) 157, 385, 613, 660, 841, 888, 1069, 1297
BstV2I GAAGAC 3 cut(s) 266, 494, 1163
BstX2I RGATCY 4 cut(s) 6, 315, 543, 1146
BstXI CCANNNNNNTGG 3 cut(s) 727, 955, 1183
BstYI RGATCY 4 cut(s) 6, 315, 543, 1146
BsuI GTATCC 3 cut(s) 283, 511, 1195
BsuRI GGCC 4 cut(s) 159, 767, 995, 1223
BtgI CCRYGG 1 cut(s) 220
Cac8I GCNNGC 6 cut(s) 139, 143, 823, 827, 1051, 1055
CaiI CAGNNNCTG 1 cut(s) 127
Cfr10I RCCGGY 2 cut(s) 593, 1277
Cfr13I GGNCC 2 cut(s) 846, 1074
CseI GACGC 1 cut(s) 624
DpnI GATC 7 cut(s) 8, 131, 317, 545, 815, 1043, 1148
DpnII GATC 7 cut(s) 6, 129, 315, 543, 813, 1041, 1146
DraIII CACNNNGTG 4 cut(s) 208, 436, 892, 1120
EaeI YGGCCR 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 270, 498
Eco47I GGWCC 2 cut(s) 846, 1074
Eco88I CYCGRG 4 cut(s) 44, 833, 1061, 1289
EcoO109I RGGNCCY 2 cut(s) 846, 1074
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 4 cut(s) 318, 546, 774, 1002
EcoT14I CCWWGG 2 cut(s) 270, 498
ErhI CCWWGG 2 cut(s) 270, 498
Esp3I CGTCTC 2 cut(s) 221, 1361
FaiI YATR 7 cut(s) 230, 458, 633, 686, 861, 914, 1178
FblI GTMKAC 1 cut(s) 1334
Fnu4HI GCNGC 9 cut(s) 146, 160, 374, 602, 674, 830, 902, 1058, 1286
Fsp4HI GCNGC 9 cut(s) 146, 160, 374, 602, 674, 830, 902, 1058, 1286
GluI GCNGC 9 cut(s) 146, 160, 374, 602, 674, 830, 902, 1058, 1286
GsuI CTGGAG 1 cut(s) 1236
HaeIII GGCC 4 cut(s) 159, 767, 995, 1223
HapII CCGG 7 cut(s) 255, 594, 670, 711, 898, 939, 1278
HgaI GACGC 1 cut(s) 624
HpaII CCGG 7 cut(s) 255, 594, 670, 711, 898, 939, 1278
HphI GGTGA 4 cut(s) 31, 259, 487, 1334
Hpy166II GTNNAC 5 cut(s) 195, 423, 879, 1107, 1335
Hpy188III TCNNGA 9 cut(s) 13, 92, 184, 244, 412, 728, 956, 1232, 1253
Hpy8I GTNNAC 5 cut(s) 195, 423, 879, 1107, 1335
HpyCH4IV ACGT 1 cut(s) 1181
HpyF10VI GCNNNNNNNGC 6 cut(s) 664, 673, 892, 901, 1120, 1129
HpySE526I ACGT 1 cut(s) 1181
Kzo9I GATC 7 cut(s) 6, 129, 315, 543, 813, 1041, 1146
LmnI GCTCC 3 cut(s) 444, 672, 900
Lsp1109I GCAGC 8 cut(s) 157, 385, 613, 660, 841, 888, 1069, 1297
LweI GCATC 1 cut(s) 1131
MaeII ACGT 1 cut(s) 1181
MalI GATC 7 cut(s) 8, 131, 317, 545, 815, 1043, 1148
MboI GATC 7 cut(s) 6, 129, 315, 543, 813, 1041, 1146
MboII GAAGA 3 cut(s) 271, 499, 1168
MfeI CAATTG 1 cut(s) 1206
MflI RGATCY 4 cut(s) 6, 315, 543, 1146
MhlI GDGCHC 3 cut(s) 441, 669, 897
MluCI AATT 2 cut(s) 333, 1206
MlyI GAGTC 8 cut(s) 200, 428, 646, 884, 1112, 1153, 1297, 1340
MmeI TCCRAC 2 cut(s) 466, 1164
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 3 cut(s) 471, 762, 990
MspI CCGG 7 cut(s) 255, 594, 670, 711, 898, 939, 1278
MspR9I CCNGG 7 cut(s) 256, 320, 548, 712, 776, 940, 1004
MunI CAATTG 1 cut(s) 1206
Mva1269I GAATGC 3 cut(s) 105, 561, 1245
MvaI CCWGG 4 cut(s) 320, 548, 776, 1004
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 6 cut(s) 664, 673, 892, 901, 1120, 1129
NciI CCSGG 3 cut(s) 256, 712, 940
NdeII GATC 7 cut(s) 6, 129, 315, 543, 813, 1041, 1146
NlaIV GGNNCC 2 cut(s) 848, 1076
PaeR7I CTCGAG 4 cut(s) 44, 833, 1061, 1289
PctI GAATGC 3 cut(s) 105, 561, 1245
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 3 cut(s) 88, 647, 1228
PflFI GACNNNGTC 3 cut(s) 74, 302, 530
PfoI TCCNGGA 4 cut(s) 318, 546, 774, 1002
PkrI GCNGC 9 cut(s) 147, 161, 375, 603, 675, 831, 903, 1059, 1287
PleI GAGTC 8 cut(s) 199, 427, 646, 883, 1111, 1153, 1297, 1339
PpsI GAGTC 8 cut(s) 199, 427, 646, 883, 1111, 1153, 1297, 1339
PpuMI RGGWCCY 2 cut(s) 846, 1074
Psp1406I AACGTT 1 cut(s) 1181
Psp5II RGGWCCY 2 cut(s) 846, 1074
Psp6I CCWGG 4 cut(s) 318, 546, 774, 1002
PspGI CCWGG 4 cut(s) 318, 546, 774, 1002
PspN4I GGNNCC 2 cut(s) 848, 1076
PspPI GGNCC 2 cut(s) 846, 1074
PspPPI RGGWCCY 2 cut(s) 846, 1074
PspXI VCTCGAGB 4 cut(s) 44, 833, 1061, 1289
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 4 cut(s) 6, 315, 543, 1146
PsyI GACNNNGTC 3 cut(s) 74, 302, 530
SaqAI TTAA 3 cut(s) 471, 762, 990
SatI GCNGC 9 cut(s) 146, 160, 374, 602, 674, 830, 902, 1058, 1286
Sau3AI GATC 7 cut(s) 6, 129, 315, 543, 813, 1041, 1146
Sau96I GGNCC 2 cut(s) 846, 1074
SchI GAGTC 8 cut(s) 200, 428, 646, 884, 1112, 1153, 1297, 1340
ScrFI CCNGG 7 cut(s) 256, 320, 548, 712, 776, 940, 1004
SduI GDGCHC 3 cut(s) 441, 669, 897
SfaNI GCATC 1 cut(s) 1131
Sfr274I CTCGAG 4 cut(s) 44, 833, 1061, 1289
SinI GGWCC 2 cut(s) 846, 1074
SlaI CTCGAG 4 cut(s) 44, 833, 1061, 1289
SmlI CTYRAG 7 cut(s) 44, 149, 377, 605, 833, 1061, 1289
SmoI CTYRAG 7 cut(s) 44, 149, 377, 605, 833, 1061, 1289
Sse9I AATT 2 cut(s) 333, 1206
SsiI CCGC 2 cut(s) 160, 450
StyD4I CCNGG 7 cut(s) 254, 318, 546, 710, 774, 938, 1002
StyI CCWWGG 2 cut(s) 270, 498
TaiI ACGT 1 cut(s) 1184
TaqI TCGA 6 cut(s) 45, 650, 834, 1062, 1126, 1290
TasI AATT 2 cut(s) 333, 1206
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 3 cut(s) 88, 647, 1228
Tru1I TTAA 3 cut(s) 471, 762, 990
Tru9I TTAA 3 cut(s) 471, 762, 990
TseI GCWGC 8 cut(s) 145, 373, 601, 673, 829, 901, 1057, 1285
TspGWI ACGGA 1 cut(s) 209
Tth111I GACNNNGTC 3 cut(s) 74, 302, 530
VpaK11BI GGWCC 2 cut(s) 846, 1074
XapI RAATTY 1 cut(s) 333
XcmI CCANNNNNNNNNTGG 4 cut(s) 202, 430, 886, 1114
XhoI CTCGAG 4 cut(s) 44, 833, 1061, 1289
XmiI GTMKAC 1 cut(s) 1334
XmnI GAANNNNTTC 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.