Prupe.6G008100_v2.0.a1

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0006959
Physical Location & Seq
Forward (+)
661251 .. 665191
3941 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G008100.4

Sequence Viewer

Length: 720 bp
ATGACCATGATTGGTGCTCCGAAAACCTCTGTCATAGTCCCTCAACATCAAAGGGGGCCCCAGACTTCGACCGGTGGATCTTATCAGAACAAGGCTTCAGGATCTCTTCCGGAGAAGAAGTACACTCACTGTGGAGGAGACAAACATACCAGTACTAGCTGTTACGAATTGATTGGAACTCATCTCCTTCTCTCTCTCTCTCTATCTCAATTCAAGCCCTCTCTCTGTCTCTCTCAAGGATCGATCTTTGTGAAAAGCGTCATTAGAGCTTCGAACCCGAGCGCAGCCACTGAGTCTTCTTCACCTGGACTCTACTCCGCCAAGAAGTACGAGCTCACCGTTCCTAACGTCGACGTGGTTCTTGAGGACGTCCGCCCCTATCTCATCGCTGATGGCGGTAACGTCGACGTTGTGTCCGTTGAGGACGGCGTCGTTTCACTCAAGCTACAAGTACATTTACAACTTGTATGTGCAATATGTCGTGAAGAACCCGCTCTACACTTCTGGAACTCCAATCAGGTTAGAGCATACTTACAGCGTCAATGGAAGCAGATTCTATCTGGAAGGGTGTCTCTTCAGGATTTTGTATCTGCAAAGGAGGTTCGCCTAGGCACCTACCAACTAGCTCTGTTCTTGTTTTGGCAGGATAAACAAACAATGTTATTGTTAATGGGAAATAGCAGCAGCCCATCATTAGCGAAGATTCAAAGGAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

240

Amino Acids

26.24

Weight (kDa)

9.18

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 611
AccBSI CCGCTC 1 cut(s) 494
AccI GTMKAC 2 cut(s) 351, 405
AccIII TCCGGA 1 cut(s) 109
AciI CCGC 4 cut(s) 318, 373, 396, 492
AclWI GGATC 3 cut(s) 85, 109, 247
AcuI CTGAAG 2 cut(s) 81, 560
AcyI GRCGYC 2 cut(s) 369, 429
AfaI GTAC 4 cut(s) 122, 154, 329, 453
AgeI ACCGGT 1 cut(s) 71
AgsI TTSAA 2 cut(s) 214, 707
AhdI GACNNNNNGTC 1 cut(s) 412
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 6 cut(s) 159, 269, 334, 445, 626, 715
AluI AGCT 6 cut(s) 159, 269, 334, 445, 626, 715
Alw21I GWGCWC 2 cut(s) 19, 336
Alw26I GTCTC 3 cut(s) 132, 233, 576
AlwI GGATC 3 cut(s) 85, 109, 247
AlwNI CAGNNNCTG 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 277
Aor13HI TCCGGA 1 cut(s) 109
AoxI GGCC 1 cut(s) 56
ApaI GGGCCC 1 cut(s) 60
ApeKI GCWGC 3 cut(s) 284, 681, 684
AsiGI ACCGGT 1 cut(s) 71
AspA2I CCTAGG 1 cut(s) 607
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 56, 57
AsuHPI GGTGA 2 cut(s) 294, 328
AsuII TTCGAA 1 cut(s) 272
AvaI CYCGRG 1 cut(s) 277
AvrII CCTAGG 1 cut(s) 607
BaeGI GKGCMC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 611
BanII GRGCYC 2 cut(s) 60, 336
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 2 cut(s) 19, 336
BbvI GCAGC 3 cut(s) 296, 693, 696
BccI CCATC 2 cut(s) 386, 697
BceAI ACGGC 1 cut(s) 442
BciT130I CCWGG 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 132, 233, 576
BfaI CTAG 3 cut(s) 156, 608, 623
BisI GCNGC 3 cut(s) 285, 682, 685
BlnI CCTAGG 1 cut(s) 607
BlsI GCNGC 3 cut(s) 286, 683, 686
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 306
BmeRI GACNNNNNGTC 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 277
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 2 cut(s) 56, 57
BmiI GGNNCC 4 cut(s) 57, 58, 59, 613
BmrFI CCNGG 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 288
Bpu14I TTCGAA 1 cut(s) 272
BpuEI CTTGAG 3 cut(s) 219, 383, 425
Bsa29I ATCGAT 1 cut(s) 242
BsaHI GRCGYC 2 cut(s) 369, 429
BsaJI CCNNGG 1 cut(s) 607
BsaWI WCCGGW 2 cut(s) 71, 109
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 150
BseAI TCCGGA 1 cut(s) 109
BseBI CCWGG 1 cut(s) 306
BseCI ATCGAT 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 607
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 1 cut(s) 150
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 3 cut(s) 296, 693, 696
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 1 cut(s) 58
BshNI GGYRCC 1 cut(s) 611
BshTI ACCGGT 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 242
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 2 cut(s) 19, 336
BsiHKCI CYCGRG 1 cut(s) 277
BsiSI CCGG 2 cut(s) 72, 110
BslFI GGGAC 1 cut(s) 23
BsmAI GTCTC 3 cut(s) 132, 233, 576
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 1 cut(s) 58
BsoBI CYCGRG 1 cut(s) 277
Bsp119I TTCGAA 1 cut(s) 272
Bsp120I GGGCCC 1 cut(s) 56
Bsp1286I GDGCHC 3 cut(s) 19, 60, 336
Bsp13I TCCGGA 1 cut(s) 109
Bsp143I GATC 4 cut(s) 77, 101, 239, 243
BspACI CCGC 4 cut(s) 318, 373, 396, 492
BspANI GGCC 1 cut(s) 58
BspCNI CTCAG 1 cut(s) 283
BspDI ATCGAT 1 cut(s) 242
BspEI TCCGGA 1 cut(s) 109
BspLI GGNNCC 4 cut(s) 57, 58, 59, 613
BspPI GGATC 3 cut(s) 85, 109, 247
BspT104I TTCGAA 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 611
BsrBI CCGCTC 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 4 cut(s) 77, 101, 239, 243
BssNI GRCGYC 2 cut(s) 369, 429
BssT1I CCWWGG 1 cut(s) 607
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 131, 340
Bst6I CTCTTC 2 cut(s) 111, 579
BstACI GRCGYC 2 cut(s) 369, 429
BstBI TTCGAA 1 cut(s) 272
BstDEI CTNAG 1 cut(s) 291
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 4 cut(s) 80, 104, 242, 246
BstMAI GTCTC 3 cut(s) 132, 233, 576
BstMBI GATC 4 cut(s) 77, 101, 239, 243
BstMCI CGRYCG 1 cut(s) 72
BstNI CCWGG 1 cut(s) 306
BstSCI CCNGG 1 cut(s) 304
BstSLI GKGCMC 1 cut(s) 60
BstV1I GCAGC 3 cut(s) 296, 693, 696
BstV2I GAAGAC 1 cut(s) 288
BstX2I RGATCY 2 cut(s) 77, 101
BstYI RGATCY 2 cut(s) 77, 101
Bsu15I ATCGAT 1 cut(s) 242
BsuRI GGCC 1 cut(s) 58
BsuTUI ATCGAT 1 cut(s) 242
BtgZI GCGATG 1 cut(s) 370
BtrI CACGTC 1 cut(s) 355
BtsIMutI CAGTG 2 cut(s) 127, 288
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 2 cut(s) 56, 57
ClaI ATCGAT 1 cut(s) 242
CseI GACGC 3 cut(s) 247, 418, 527
Csp6I GTAC 4 cut(s) 121, 153, 328, 452
CspAI ACCGGT 1 cut(s) 71
CviAII CATG 1 cut(s) 7
CviQI GTAC 4 cut(s) 121, 153, 328, 452
DdeI CTNAG 1 cut(s) 291
DpnI GATC 4 cut(s) 79, 103, 241, 245
DpnII GATC 4 cut(s) 77, 101, 239, 243
DriI GACNNNNNGTC 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 111, 579
Eam1105I GACNNNNNGTC 1 cut(s) 412
EarI CTCTTC 2 cut(s) 111, 579
EciI GGCGGA 2 cut(s) 307, 362
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 607
Eco24I GRGCYC 2 cut(s) 60, 336
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 2 cut(s) 81, 560
Eco88I CYCGRG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 334
EcoO109I RGGNCCY 2 cut(s) 56, 57
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 607
EcoT38I GRGCYC 2 cut(s) 60, 336
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 1 cut(s) 10
FaiI YATR 7 cut(s) 8, 35, 147, 469, 478, 529, 718
FaqI GGGAC 1 cut(s) 23
FatI CATG 1 cut(s) 6
FauI CCCGC 1 cut(s) 499
FblI GTMKAC 2 cut(s) 351, 405
Fnu4HI GCNGC 3 cut(s) 285, 682, 685
FriOI GRGCYC 2 cut(s) 60, 336
Fsp4HI GCNGC 3 cut(s) 285, 682, 685
FspBI CTAG 3 cut(s) 156, 608, 623
GlaI GCGC 1 cut(s) 283
GluI GCNGC 3 cut(s) 285, 682, 685
HaeIII GGCC 1 cut(s) 58
HapII CCGG 2 cut(s) 72, 110
HgaI GACGC 3 cut(s) 247, 418, 527
HhaI GCGC 1 cut(s) 284
Hin1I GRCGYC 2 cut(s) 369, 429
Hin1II CATG 1 cut(s) 10
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 2 cut(s) 352, 406
HindII GTYRAC 2 cut(s) 352, 406
HinfI GANTC 4 cut(s) 293, 309, 553, 703
HpaII CCGG 2 cut(s) 72, 110
HphI GGTGA 2 cut(s) 294, 328
Hpy166II GTNNAC 3 cut(s) 123, 352, 406
Hpy188I TCNGA 2 cut(s) 21, 87
Hpy188III TCNNGA 7 cut(s) 99, 110, 362, 482, 505, 561, 578
Hpy8I GTNNAC 3 cut(s) 123, 352, 406
Hpy99I CGWCG 5 cut(s) 353, 356, 407, 410, 434
HpyAV CCTTC 2 cut(s) 197, 558
HpyCH4III ACNGT 2 cut(s) 131, 340
HpyCH4IV ACGT 5 cut(s) 348, 354, 369, 402, 408
HpyCH4V TGCA 2 cut(s) 473, 593
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 5 cut(s) 348, 354, 369, 402, 408
Hsp92I GRCGYC 2 cut(s) 369, 429
Hsp92II CATG 1 cut(s) 10
HspAI GCGC 1 cut(s) 282
Kpn2I TCCGGA 1 cut(s) 109
Kzo9I GATC 4 cut(s) 77, 101, 239, 243
LmnI GCTCC 1 cut(s) 22
Lsp1109I GCAGC 3 cut(s) 296, 693, 696
MaeI CTAG 3 cut(s) 156, 608, 623
MaeII ACGT 5 cut(s) 348, 354, 369, 402, 408
MaeIII GTNAC 2 cut(s) 161, 398
MalI GATC 4 cut(s) 79, 103, 241, 245
MbiI CCGCTC 1 cut(s) 494
MboI GATC 4 cut(s) 77, 101, 239, 243
MboII GAAGA 7 cut(s) 98, 127, 288, 291, 497, 566, 712
MflI RGATCY 2 cut(s) 77, 101
MhlI GDGCHC 3 cut(s) 19, 60, 336
MluCI AATT 2 cut(s) 167, 209
MlyI GAGTC 2 cut(s) 302, 303
MnlI CCTC 7 cut(s) 37, 51, 128, 229, 358, 415, 592
MroI TCCGGA 1 cut(s) 109
MseI TTAA 1 cut(s) 668
MspI CCGG 2 cut(s) 72, 110
MspR9I CCNGG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 306
NdeII GATC 4 cut(s) 77, 101, 239, 243
NlaIII CATG 1 cut(s) 10
NlaIV GGNNCC 4 cut(s) 57, 58, 59, 613
NspV TTCGAA 1 cut(s) 272
PcsI WCGNNNNNNNCGW 2 cut(s) 336, 414
PfeI GAWTC 2 cut(s) 553, 703
PflFI GACNNNGTC 1 cut(s) 428
PinAI ACCGGT 1 cut(s) 71
PkrI GCNGC 3 cut(s) 286, 683, 686
PleI GAGTC 2 cut(s) 301, 303
PpsI GAGTC 2 cut(s) 301, 303
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 4 cut(s) 57, 58, 59, 613
PspOMI GGGCCC 1 cut(s) 56
PspPI GGNCC 2 cut(s) 56, 57
PsrI GAACNNNNNNTAC 2 cut(s) 480, 512
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 2 cut(s) 77, 101
PsyI GACNNNGTC 1 cut(s) 428
RsaI GTAC 4 cut(s) 122, 154, 329, 453
RsaNI GTAC 4 cut(s) 121, 153, 328, 452
SacI GAGCTC 1 cut(s) 336
SalI GTCGAC 2 cut(s) 350, 404
SaqAI TTAA 1 cut(s) 668
SatI GCNGC 3 cut(s) 285, 682, 685
Sau3AI GATC 4 cut(s) 77, 101, 239, 243
Sau96I GGNCC 2 cut(s) 56, 57
ScaI AGTACT 1 cut(s) 154
SchI GAGTC 2 cut(s) 302, 303
ScrFI CCNGG 1 cut(s) 306
SduI GDGCHC 3 cut(s) 19, 60, 336
SfuI TTCGAA 1 cut(s) 272
SgrDI CGTCGACG 2 cut(s) 350, 404
SmlI CTYRAG 3 cut(s) 234, 362, 440
SmoI CTYRAG 3 cut(s) 234, 362, 440
Sse9I AATT 2 cut(s) 167, 209
SsiI CCGC 4 cut(s) 318, 373, 396, 492
SspMI CTAG 3 cut(s) 156, 608, 623
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 304
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 2 cut(s) 131, 340
TaiI ACGT 5 cut(s) 351, 357, 372, 405, 411
TaqI TCGA 5 cut(s) 68, 242, 272, 351, 405
TasI AATT 2 cut(s) 167, 209
TatI WGTACW 3 cut(s) 120, 152, 451
TfiI GAWTC 2 cut(s) 553, 703
Tru1I TTAA 1 cut(s) 668
Tru9I TTAA 1 cut(s) 668
TscAI CASTG 2 cut(s) 134, 295
TseI GCWGC 3 cut(s) 284, 681, 684
TspGWI ACGGA 1 cut(s) 406
TspRI CASTG 2 cut(s) 134, 295
Tth111I GACNNNGTC 1 cut(s) 428
XmaJI CCTAGG 1 cut(s) 607
XmiI GTMKAC 2 cut(s) 351, 405
XspI CTAG 3 cut(s) 156, 608, 623
ZraI GACGTC 1 cut(s) 370
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.