DNA polymerase activity
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 260 |
| AclWI | GGATC | 2 cut(s) | 109, 122 |
| AcsI | RAATTY | 1 cut(s) | 36 |
| AcuI | CTGAAG | 1 cut(s) | 209 |
| AgsI | TTSAA | 1 cut(s) | 356 |
| AluBI | AGCT | 2 cut(s) | 275, 364 |
| AluI | AGCT | 2 cut(s) | 275, 364 |
| Alw26I | GTCTC | 1 cut(s) | 225 |
| AlwI | GGATC | 2 cut(s) | 109, 122 |
| ApeKI | GCWGC | 2 cut(s) | 330, 333 |
| ApoI | RAATTY | 1 cut(s) | 36 |
| AspA2I | CCTAGG | 1 cut(s) | 256 |
| AsuHPI | GGTGA | 2 cut(s) | 136, 150 |
| AvrII | CCTAGG | 1 cut(s) | 256 |
| BamHI | GGATCC | 1 cut(s) | 114 |
| BanI | GGYRCC | 1 cut(s) | 260 |
| BbvI | GCAGC | 2 cut(s) | 342, 345 |
| BccI | CCATC | 2 cut(s) | 126, 346 |
| BceAI | ACGGC | 1 cut(s) | 3 |
| BcoDI | GTCTC | 1 cut(s) | 225 |
| BfaI | CTAG | 2 cut(s) | 257, 272 |
| BisI | GCNGC | 2 cut(s) | 331, 334 |
| BlnI | CCTAGG | 1 cut(s) | 256 |
| BlsI | GCNGC | 2 cut(s) | 332, 335 |
| BmiI | GGNNCC | 2 cut(s) | 116, 262 |
| BmsI | GCATC | 2 cut(s) | 74, 145 |
| BsaJI | CCNNGG | 1 cut(s) | 256 |
| BseDI | CCNNGG | 1 cut(s) | 256 |
| BseXI | GCAGC | 2 cut(s) | 342, 345 |
| BseYI | CCCAGC | 1 cut(s) | 110 |
| BshNI | GGYRCC | 1 cut(s) | 260 |
| BslFI | GGGAC | 1 cut(s) | 95 |
| BsmAI | GTCTC | 1 cut(s) | 225 |
| BsmFI | GGGAC | 1 cut(s) | 95 |
| Bsp143I | GATC | 1 cut(s) | 114 |
| BspHI | TCATGA | 1 cut(s) | 58 |
| BspLI | GGNNCC | 2 cut(s) | 116, 262 |
| BspPI | GGATC | 2 cut(s) | 109, 122 |
| BspT107I | GGYRCC | 1 cut(s) | 260 |
| BssECI | CCNNGG | 1 cut(s) | 256 |
| BssMI | GATC | 1 cut(s) | 114 |
| BssT1I | CCWWGG | 1 cut(s) | 256 |
| Bst6I | CTCTTC | 1 cut(s) | 228 |
| BstEII | GGTNACC | 1 cut(s) | 156 |
| BstKTI | GATC | 1 cut(s) | 117 |
| BstMAI | GTCTC | 1 cut(s) | 225 |
| BstMBI | GATC | 1 cut(s) | 114 |
| BstPI | GGTNACC | 1 cut(s) | 156 |
| BstV1I | GCAGC | 2 cut(s) | 342, 345 |
| BstX2I | RGATCY | 1 cut(s) | 114 |
| BstYI | RGATCY | 1 cut(s) | 114 |
| CciI | TCATGA | 1 cut(s) | 58 |
| CseI | GACGC | 1 cut(s) | 176 |
| CviAII | CATG | 2 cut(s) | 59, 153 |
| CviJI | RGCY | 4 cut(s) | 93, 275, 336, 364 |
| CviKI_1 | RGCY | 4 cut(s) | 93, 275, 336, 364 |
| DpnI | GATC | 1 cut(s) | 116 |
| DpnII | GATC | 1 cut(s) | 114 |
| Eam1104I | CTCTTC | 1 cut(s) | 228 |
| EarI | CTCTTC | 1 cut(s) | 228 |
| Eco130I | CCWWGG | 1 cut(s) | 256 |
| Eco57I | CTGAAG | 1 cut(s) | 209 |
| Eco91I | GGTNACC | 1 cut(s) | 156 |
| EcoO65I | GGTNACC | 1 cut(s) | 156 |
| EcoT14I | CCWWGG | 1 cut(s) | 256 |
| ErhI | CCWWGG | 1 cut(s) | 256 |
| FaeI | CATG | 2 cut(s) | 62, 156 |
| FaqI | GGGAC | 1 cut(s) | 95 |
| FatI | CATG | 2 cut(s) | 58, 152 |
| Fnu4HI | GCNGC | 2 cut(s) | 331, 334 |
| Fsp4HI | GCNGC | 2 cut(s) | 331, 334 |
| FspBI | CTAG | 2 cut(s) | 257, 272 |
| GluI | GCNGC | 2 cut(s) | 331, 334 |
| GsaI | CCCAGC | 1 cut(s) | 114 |
| HgaI | GACGC | 1 cut(s) | 176 |
| Hin1II | CATG | 2 cut(s) | 62, 156 |
| HinfI | GANTC | 2 cut(s) | 202, 352 |
| HphI | GGTGA | 2 cut(s) | 136, 150 |
| Hpy188I | TCNGA | 1 cut(s) | 12 |
| Hpy188III | TCNNGA | 3 cut(s) | 59, 210, 227 |
| HpyAV | CCTTC | 1 cut(s) | 207 |
| HpyCH4V | TGCA | 2 cut(s) | 136, 242 |
| Hsp92II | CATG | 2 cut(s) | 62, 156 |
| Kzo9I | GATC | 1 cut(s) | 114 |
| LpnPI | CCDG | 5 cut(s) | 96, 107, 195, 212, 278 |
| Lsp1109I | GCAGC | 2 cut(s) | 342, 345 |
| LweI | GCATC | 2 cut(s) | 74, 145 |
| MaeI | CTAG | 2 cut(s) | 257, 272 |
| MaeIII | GTNAC | 1 cut(s) | 156 |
| MalI | GATC | 1 cut(s) | 116 |
| MboI | GATC | 1 cut(s) | 114 |
| MboII | GAAGA | 2 cut(s) | 215, 361 |
| MflI | RGATCY | 1 cut(s) | 114 |
| MluCI | AATT | 2 cut(s) | 36, 145 |
| MnlI | CCTC | 3 cut(s) | 16, 72, 241 |
| MseI | TTAA | 1 cut(s) | 317 |
| NdeII | GATC | 1 cut(s) | 114 |
| NlaIII | CATG | 2 cut(s) | 62, 156 |
| NlaIV | GGNNCC | 2 cut(s) | 116, 262 |
| NmuCI | GTSAC | 1 cut(s) | 156 |
| PagI | TCATGA | 1 cut(s) | 58 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 16 |
| PfeI | GAWTC | 2 cut(s) | 202, 352 |
| PkrI | GCNGC | 2 cut(s) | 332, 335 |
| PspEI | GGTNACC | 1 cut(s) | 156 |
| PspFI | CCCAGC | 1 cut(s) | 110 |
| PspN4I | GGNNCC | 2 cut(s) | 116, 262 |
| PsuI | RGATCY | 1 cut(s) | 114 |
| SaqAI | TTAA | 1 cut(s) | 317 |
| SatI | GCNGC | 2 cut(s) | 331, 334 |
| Sau3AI | GATC | 1 cut(s) | 114 |
| SetI | ASST | 5 cut(s) | 171, 252, 266, 277, 366 |
| SfaNI | GCATC | 2 cut(s) | 74, 145 |
| Sse9I | AATT | 2 cut(s) | 36, 145 |
| SspMI | CTAG | 2 cut(s) | 257, 272 |
| StyI | CCWWGG | 1 cut(s) | 256 |
| TasI | AATT | 2 cut(s) | 36, 145 |
| TfiI | GAWTC | 2 cut(s) | 202, 352 |
| Tru1I | TTAA | 1 cut(s) | 317 |
| Tru9I | TTAA | 1 cut(s) | 317 |
| TseFI | GTSAC | 1 cut(s) | 156 |
| TseI | GCWGC | 2 cut(s) | 330, 333 |
| Tsp45I | GTSAC | 1 cut(s) | 156 |
| TspDTI | ATGAA | 2 cut(s) | 29, 75 |
| XapI | RAATTY | 1 cut(s) | 36 |
| XmaJI | CCTAGG | 1 cut(s) | 256 |
| XspI | CTAG | 2 cut(s) | 257, 272 |