Prupe.6G029200_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0020414
Physical Location & Seq
Reverse (-)
2259268 .. 2260107
840 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G029200.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 693 bp
ATGCTCTCCCCAACACCATTTTCTCTCATTTGCGCTGTTCTTCTATATGACAACTCGCTCTTCCACCTCGCCGCCCGTGCCATGTTCGCGCTTCTCTCTCCTTCTTGCATCCTCATCTGGTCCTTCAACTTCACGTACCAAACCCTCACCCGATCGAAGAAGAGTTTCATCGAGATTCTAGATAACGAGATCGGGGCGTATGACAGGTGGGCAGCGCGTGGGGAGGACGCAATGCTGCCGCATGTGAAGCTAGAGGAGTTTCGGATCGGGTCTCAATTCTGGATCCTGAAGCGGAAGCACGTGAGGGTGGTTGTGGGTGACCACCGGCTCTGGTCTAAGTTCAAGCTACCGTGCCAGCGTTGGGACACATGTTACCCAGAGGAAAATTACTTTCCTACCCTCCTGAACATGAGAGAACCAGGCGGGTGCGTGCCCGCTACCCTTATGCACGTGGATTGGCGCGGGAGATTCGATGGCCACCCCCGAACATATGAGGCCTCCGACTTGGGGCCTGATCTGATCACCTCCCTGAGAAATGACAGGCCGAAATACGATGACGAGAAGGAGAGCGACAACGGCTCTGATTGGTCCTTGACGAAACTGCGAGACCCGTTTCTGTTCGCAAGCAAGTTCCCGCCGGATGCGATTGGACTGTTGATGAGTATGGCCAGTGATGTCATCTTCAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

231

Amino Acids

26.61

Weight (kDa)

6.31

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020414)

Species Orthologous Gene IDs
prunus_persica Prupe.6G029200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0196261 RchiOBHm_Chr7g0233281
rosa_multiflora Rmu_sc0018617.1_g000001
rosa_roxburghii Rroxscaffold_1G00002260
rosa_samantha Rh7CG377500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 89, 217, 462
AciI CCGC 7 cut(s) 72, 239, 292, 423, 435, 462, 635
AclWI GGATC 3 cut(s) 272, 277, 290
AcoI YGGCCR 2 cut(s) 475, 666
AcuI CTGAAG 1 cut(s) 308
AcvI CACGTG 2 cut(s) 301, 451
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 361, 507
AflIII ACRYGT 1 cut(s) 368
AgsI TTSAA 3 cut(s) 127, 343, 685
AjnI CCWGG 1 cut(s) 418
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AluBI AGCT 2 cut(s) 250, 346
AluI AGCT 2 cut(s) 250, 346
Alw26I GTCTC 2 cut(s) 276, 600
AlwI GGATC 3 cut(s) 272, 277, 290
AoxI GGCC 5 cut(s) 475, 495, 509, 542, 666
ApeKI GCWGC 2 cut(s) 212, 235
Asp700I GAANNNNTTC 1 cut(s) 164
AspLEI GCGC 4 cut(s) 35, 91, 217, 462
AspS9I GGNCC 3 cut(s) 120, 509, 588
AsuHPI GGTGA 3 cut(s) 139, 329, 514
AvaII GGWCC 2 cut(s) 120, 588
BaeGI GKGCMC 1 cut(s) 435
BalI TGGCCA 2 cut(s) 477, 668
BamHI GGATCC 1 cut(s) 282
BbrPI CACGTG 2 cut(s) 301, 451
BbvI GCAGC 2 cut(s) 222, 224
BccI CCATC 1 cut(s) 467
BceAI ACGGC 1 cut(s) 592
BciT130I CCWGG 1 cut(s) 420
BclI TGATCA 1 cut(s) 519
BcoDI GTCTC 2 cut(s) 276, 600
BfaI CTAG 2 cut(s) 179, 251
BisI GCNGC 4 cut(s) 72, 213, 236, 239
BlsI GCNGC 4 cut(s) 73, 214, 237, 240
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 2 cut(s) 120, 588
BmgT120I GGNCC 3 cut(s) 120, 509, 588
BmiI GGNNCC 2 cut(s) 284, 510
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 2 cut(s) 117, 631
BsaAI YACGTR 3 cut(s) 135, 301, 451
BsaI GGTCTC 2 cut(s) 276, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 361, 507
Bse118I RCCGGY 1 cut(s) 324
Bse1I ACTGG 1 cut(s) 669
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 1 cut(s) 420
BseGI GGATG 2 cut(s) 108, 646
BseLI CCNNNNNNNGG 2 cut(s) 361, 507
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 669
BseRI GAGGAG 1 cut(s) 269
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 2 cut(s) 222, 224
Bsh1236I CGCG 3 cut(s) 89, 217, 462
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 5 cut(s) 477, 497, 511, 544, 668
BsiEI CGRYCG 1 cut(s) 155
BsiSI CCGG 2 cut(s) 325, 638
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 361, 507
BsmAI GTCTC 2 cut(s) 276, 600
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 5 cut(s) 477, 497, 511, 544, 668
Bso31I GGTCTC 2 cut(s) 276, 600
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 6 cut(s) 152, 189, 264, 282, 514, 519
BspACI CCGC 7 cut(s) 72, 239, 292, 423, 435, 462, 635
BspANI GGCC 5 cut(s) 477, 497, 511, 544, 668
BspCNI CTCAG 1 cut(s) 522
BspFNI CGCG 3 cut(s) 89, 217, 462
BspLI GGNNCC 2 cut(s) 284, 510
BspPI GGATC 3 cut(s) 272, 277, 290
BspQI GCTCTTC 1 cut(s) 65
BspTNI GGTCTC 2 cut(s) 276, 600
BsrDI GCAATG 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 324
BsrI ACTGG 1 cut(s) 669
BssAI RCCGGY 1 cut(s) 324
BssMI GATC 6 cut(s) 152, 189, 264, 282, 514, 519
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 2 cut(s) 351, 654
Bst6I CTCTTC 2 cut(s) 65, 155
BstBAI YACGTR 3 cut(s) 135, 301, 451
BstC8I GCNNGC 4 cut(s) 356, 431, 435, 625
BstDEI CTNAG 2 cut(s) 336, 530
BstEII GGTNACC 1 cut(s) 317
BstF5I GGATG 2 cut(s) 108, 646
BstFNI CGCG 3 cut(s) 89, 217, 462
BstHHI GCGC 4 cut(s) 35, 91, 217, 462
BstKTI GATC 6 cut(s) 155, 192, 267, 285, 517, 522
BstMAI GTCTC 2 cut(s) 276, 600
BstMBI GATC 6 cut(s) 152, 189, 264, 282, 514, 519
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 4 cut(s) 77, 86, 247, 576
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 2 cut(s) 245, 372
BstPI GGTNACC 1 cut(s) 317
BstSCI CCNGG 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 435
BstUI CGCG 3 cut(s) 89, 217, 462
BstV1I GCAGC 2 cut(s) 222, 224
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BsuRI GGCC 5 cut(s) 477, 497, 511, 544, 668
BtsCI GGATG 2 cut(s) 108, 646
BtsIMutI CAGTG 1 cut(s) 676
Cac8I GCNNGC 4 cut(s) 356, 431, 435, 625
CfoI GCGC 4 cut(s) 35, 91, 217, 462
Cfr10I RCCGGY 1 cut(s) 324
Cfr13I GGNCC 3 cut(s) 120, 509, 588
CseI GACGC 1 cut(s) 236
Csp6I GTAC 1 cut(s) 136
CviAII CATG 4 cut(s) 82, 242, 369, 409
CviJI RGCY 9 cut(s) 250, 328, 346, 477, 497, 511, 544, 579, 668
CviKI_1 RGCY 9 cut(s) 250, 328, 346, 477, 497, 511, 544, 579, 668
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 2 cut(s) 336, 530
DpnI GATC 6 cut(s) 154, 191, 266, 284, 516, 521
DpnII GATC 6 cut(s) 152, 189, 264, 282, 514, 519
EaeI YGGCCR 2 cut(s) 475, 666
Eam1104I CTCTTC 2 cut(s) 65, 155
EarI CTCTTC 2 cut(s) 65, 155
Eco147I AGGCCT 1 cut(s) 497
Eco31I GGTCTC 2 cut(s) 276, 600
Eco47I GGWCC 2 cut(s) 120, 588
Eco57I CTGAAG 1 cut(s) 308
Eco72I CACGTG 2 cut(s) 301, 451
Eco91I GGTNACC 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 509
EcoO65I GGTNACC 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 418
FaeI CATG 4 cut(s) 85, 245, 372, 412
FaqI GGGAC 1 cut(s) 377
FatI CATG 4 cut(s) 81, 241, 368, 408
FauI CCCGC 4 cut(s) 416, 442, 455, 642
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 72, 213, 236, 239
FokI GGATG 2 cut(s) 95, 653
Fsp4HI GCNGC 4 cut(s) 72, 213, 236, 239
FspBI CTAG 2 cut(s) 179, 251
GlaI GCGC 4 cut(s) 34, 90, 216, 461
GluI GCNGC 4 cut(s) 72, 213, 236, 239
HaeIII GGCC 5 cut(s) 477, 497, 511, 544, 668
HapII CCGG 2 cut(s) 325, 638
HgaI GACGC 1 cut(s) 236
HhaI GCGC 4 cut(s) 35, 91, 217, 462
Hin1II CATG 4 cut(s) 85, 245, 372, 412
Hin6I GCGC 4 cut(s) 33, 89, 215, 460
HinP1I GCGC 4 cut(s) 33, 89, 215, 460
HinfI GANTC 2 cut(s) 175, 468
HpaII CCGG 2 cut(s) 325, 638
HphI GGTGA 3 cut(s) 139, 329, 514
Hpy188I TCNGA 4 cut(s) 264, 502, 519, 583
Hpy188III TCNNGA 5 cut(s) 172, 179, 280, 286, 403
HpyAV CCTTC 3 cut(s) 111, 133, 556
HpyCH4III ACNGT 2 cut(s) 351, 654
HpyCH4IV ACGT 3 cut(s) 134, 300, 450
HpyCH4V TGCA 2 cut(s) 108, 448
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 86, 247, 576
HpyF3I CTNAG 2 cut(s) 336, 530
HpySE526I ACGT 3 cut(s) 134, 300, 450
Hsp92II CATG 4 cut(s) 85, 245, 372, 412
HspAI GCGC 4 cut(s) 33, 89, 215, 460
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 6 cut(s) 152, 189, 264, 282, 514, 519
LguI GCTCTTC 1 cut(s) 65
Lsp1109I GCAGC 2 cut(s) 222, 224
LweI GCATC 2 cut(s) 117, 631
MaeI CTAG 2 cut(s) 179, 251
MaeII ACGT 3 cut(s) 134, 300, 450
MaeIII GTNAC 2 cut(s) 317, 371
MalI GATC 6 cut(s) 154, 191, 266, 284, 516, 521
MboI GATC 6 cut(s) 152, 189, 264, 282, 514, 519
MboII GAAGA 5 cut(s) 32, 52, 169, 172, 673
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 435
MlsI TGGCCA 2 cut(s) 477, 668
MluCI AATT 2 cut(s) 275, 385
MluNI TGGCCA 2 cut(s) 477, 668
MmeI TCCRAC 1 cut(s) 525
Mox20I TGGCCA 2 cut(s) 477, 668
MroXI GAANNNNTTC 1 cut(s) 164
MscI TGGCCA 2 cut(s) 477, 668
MseI TTAA 1 cut(s) 691
Msp20I TGGCCA 2 cut(s) 477, 668
MspI CCGG 2 cut(s) 325, 638
MspR9I CCNGG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 420
MvnI CGCG 3 cut(s) 89, 217, 462
MwoI GCNNNNNNNGC 4 cut(s) 77, 86, 247, 576
NdeI CATATG 1 cut(s) 490
NdeII GATC 6 cut(s) 152, 189, 264, 282, 514, 519
NlaIII CATG 4 cut(s) 85, 245, 372, 412
NlaIV GGNNCC 2 cut(s) 284, 510
NmuCI GTSAC 1 cut(s) 317
NspI RCATGY 2 cut(s) 245, 372
PceI AGGCCT 1 cut(s) 497
PciI ACATGT 1 cut(s) 368
PciSI GCTCTTC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 175, 468
PkrI GCNGC 4 cut(s) 73, 214, 237, 240
Ple19I CGATCG 1 cut(s) 155
PmaCI CACGTG 2 cut(s) 301, 451
PmlI CACGTG 2 cut(s) 301, 451
Ppu21I YACGTR 3 cut(s) 135, 301, 451
PscI ACATGT 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 418
PspCI CACGTG 2 cut(s) 301, 451
PspEI GGTNACC 1 cut(s) 317
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 284, 510
PspPI GGNCC 3 cut(s) 120, 509, 588
PsuI RGATCY 1 cut(s) 282
PvuI CGATCG 1 cut(s) 155
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 1 cut(s) 691
SatI GCNGC 4 cut(s) 72, 213, 236, 239
Sau3AI GATC 6 cut(s) 152, 189, 264, 282, 514, 519
Sau96I GGNCC 3 cut(s) 120, 509, 588
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 1 cut(s) 435
SetI ASST 8 cut(s) 69, 137, 209, 252, 303, 348, 453, 527
SfaNI GCATC 2 cut(s) 117, 631
SinI GGWCC 2 cut(s) 120, 588
Sse9I AATT 2 cut(s) 275, 385
SseBI AGGCCT 1 cut(s) 497
SsiI CCGC 7 cut(s) 72, 239, 292, 423, 435, 462, 635
SspMI CTAG 2 cut(s) 179, 251
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 1 cut(s) 418
TaaI ACNGT 2 cut(s) 351, 654
TaiI ACGT 3 cut(s) 137, 303, 453
TaqI TCGA 3 cut(s) 155, 171, 471
TasI AATT 2 cut(s) 275, 385
TauI GCSGC 2 cut(s) 74, 241
TfiI GAWTC 2 cut(s) 175, 468
Tru1I TTAA 1 cut(s) 691
Tru9I TTAA 1 cut(s) 691
TscAI CASTG 1 cut(s) 676
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 2 cut(s) 212, 235
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 1 cut(s) 157
TspRI CASTG 1 cut(s) 676
VpaK11BI GGWCC 2 cut(s) 120, 588
XbaI TCTAGA 1 cut(s) 178
XceI RCATGY 2 cut(s) 245, 372
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 2 cut(s) 179, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.