Prupe.6G253800_v2.0.a1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0018457
Physical Location & Seq
Reverse (-)
24723202 .. 24723935
734 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G253800.1

Sequence Viewer

Length: 477 bp
ATGGCAGAGAGGAAGAGAGCCAAAACTACTTCAACTTGCAGCCCTCTGGCCACCGTTGAAGAGATTCAGAGACGCCTCGTTCGACCTTCCTCCACCTCCATTTGTCCTCCACCGACCTCAACATTCTCATCGACGGACGATTTTCAAAGCTGCCACAAGGCCCCTGACTCTTCGAAATTCAAGTTGAGAACGACGACGAAGAAGCTCGAAGATTACTTGGATTCGGTCCTGCTCTCTCAGATCTCATCCAAGCTGAGTCGCGCGAAGAAGGTTCCAGAGACGAAGTTGCAGAAGAGAGAGGTGAAGGATTTTGAGTGGCCGATCGACGAATTGAAGGTGTTGGCGGATTCGGACAAGGAAGATGTGAATCTCAAGGACGATTTGGATTTGACTGAGGAATTTGGGGCCGGCGATGTTGAATCTTGCACTCCGTTTCGGCGTTTTGAGCAAATTGCCGCGCGGCGCTTCGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

159

Amino Acids

17.94

Weight (kDa)

6.62

Isoelectric Point (pI)

68.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018457)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13961
prunus_persica Prupe.6G253800_v2.0.a1
rosa_multiflora Rmu_sc0008017.1_g000008
rosa_roxburghii Rroxscaffold_6G00414160
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131100 Rh3BG152100 Rh3CG152300 Rh3DG153300
rosa_wichuraiana Rw3G012390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 261, 263, 458, 460
AciI CCGC 3 cut(s) 344, 456, 460
AcoI YGGCCR 2 cut(s) 48, 317
AcsI RAATTY 2 cut(s) 176, 398
AcyI GRCGYC 1 cut(s) 73
AgsI TTSAA 6 cut(s) 33, 59, 146, 181, 334, 419
AluBI AGCT 3 cut(s) 150, 205, 253
AluI AGCT 3 cut(s) 150, 205, 253
Alw26I GTCTC 2 cut(s) 64, 272
AoxI GGCC 4 cut(s) 48, 159, 317, 405
ApeKI GCWGC 2 cut(s) 39, 150
ApoI RAATTY 2 cut(s) 176, 398
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 3 cut(s) 263, 460, 465
AspS9I GGNCC 3 cut(s) 160, 226, 405
AsuHPI GGTGA 1 cut(s) 313
AsuII TTCGAA 2 cut(s) 173, 468
AvaII GGWCC 1 cut(s) 226
BalI TGGCCA 1 cut(s) 50
BbvI GCAGC 2 cut(s) 51, 137
BcoDI GTCTC 2 cut(s) 64, 272
BfoI RGCGCY 1 cut(s) 466
BglII AGATCT 1 cut(s) 240
BisI GCNGC 4 cut(s) 40, 151, 456, 461
BlsI GCNGC 4 cut(s) 41, 152, 457, 462
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 160, 226, 405
BmiI GGNNCC 3 cut(s) 162, 273, 406
Bpu14I TTCGAA 2 cut(s) 173, 468
BpuEI CTTGAG 1 cut(s) 356
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 73
Bse118I RCCGGY 1 cut(s) 407
Bse8I GATNNNNATC 1 cut(s) 366
BseGI GGATG 1 cut(s) 245
BseJI GATNNNNATC 1 cut(s) 366
BseMII CTCAG 3 cut(s) 245, 251, 384
BseXI GCAGC 2 cut(s) 51, 137
Bsh1236I CGCG 4 cut(s) 261, 263, 458, 460
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 4 cut(s) 50, 161, 319, 407
BsiEI CGRYCG 1 cut(s) 324
BsiSI CCGG 1 cut(s) 408
BsmAI GTCTC 2 cut(s) 64, 272
BsmBI CGTCTC 2 cut(s) 64, 272
BsnI GGCC 4 cut(s) 50, 161, 319, 407
Bsp119I TTCGAA 2 cut(s) 173, 468
Bsp143I GATC 2 cut(s) 240, 321
BspACI CCGC 3 cut(s) 344, 456, 460
BspANI GGCC 4 cut(s) 50, 161, 319, 407
BspCNI CTCAG 3 cut(s) 246, 250, 385
BspFNI CGCG 4 cut(s) 261, 263, 458, 460
BspLI GGNNCC 3 cut(s) 162, 273, 406
BspT104I TTCGAA 2 cut(s) 173, 468
BsrFI RCCGGY 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 407
BssMI GATC 2 cut(s) 240, 321
BssNI GRCGYC 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 4 cut(s) 8, 54, 175, 287
BstACI GRCGYC 1 cut(s) 73
BstBI TTCGAA 2 cut(s) 173, 468
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 3 cut(s) 237, 254, 393
BstF5I GGATG 1 cut(s) 245
BstFNI CGCG 4 cut(s) 261, 263, 458, 460
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 3 cut(s) 263, 460, 465
BstKTI GATC 2 cut(s) 243, 324
BstMAI GTCTC 2 cut(s) 64, 272
BstMBI GATC 2 cut(s) 240, 321
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 1 cut(s) 445
BstUI CGCG 4 cut(s) 261, 263, 458, 460
BstV1I GCAGC 2 cut(s) 51, 137
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
BsuRI GGCC 4 cut(s) 50, 161, 319, 407
BtgZI GCGATG 1 cut(s) 426
BtsCI GGATG 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 409
CfoI GCGC 3 cut(s) 263, 460, 465
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 3 cut(s) 160, 226, 405
CseI GACGC 1 cut(s) 81
CviJI RGCY 9 cut(s) 20, 42, 50, 150, 161, 205, 253, 319, 407
CviKI_1 RGCY 9 cut(s) 20, 42, 50, 150, 161, 205, 253, 319, 407
DdeI CTNAG 3 cut(s) 237, 254, 393
DpnI GATC 2 cut(s) 242, 323
DpnII GATC 2 cut(s) 240, 321
EaeI YGGCCR 2 cut(s) 48, 317
Eam1104I CTCTTC 4 cut(s) 8, 54, 175, 287
EarI CTCTTC 4 cut(s) 8, 54, 175, 287
EciI GGCGGA 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 160
Esp3I CGTCTC 2 cut(s) 64, 272
Fnu4HI GCNGC 4 cut(s) 40, 151, 456, 461
FokI GGATG 1 cut(s) 232
Fsp4HI GCNGC 4 cut(s) 40, 151, 456, 461
GlaI GCGC 3 cut(s) 262, 459, 464
GluI GCNGC 4 cut(s) 40, 151, 456, 461
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 4 cut(s) 50, 161, 319, 407
HapII CCGG 1 cut(s) 408
HgaI GACGC 1 cut(s) 81
HhaI GCGC 3 cut(s) 263, 460, 465
Hin1I GRCGYC 1 cut(s) 73
Hin6I GCGC 3 cut(s) 261, 458, 463
HinP1I GCGC 3 cut(s) 261, 458, 463
HinfI GANTC 7 cut(s) 64, 167, 221, 256, 347, 367, 419
HpaII CCGG 1 cut(s) 408
HphI GGTGA 1 cut(s) 313
Hpy188I TCNGA 3 cut(s) 69, 240, 352
Hpy188III TCNNGA 1 cut(s) 275
Hpy99I CGWCG 4 cut(s) 136, 196, 199, 329
HpyAV CCTTC 5 cut(s) 96, 262, 298, 328, 464
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4V TGCA 3 cut(s) 39, 289, 426
HpyF10VI GCNNNNNNNGC 1 cut(s) 445
HpyF3I CTNAG 3 cut(s) 237, 254, 393
Hsp92I GRCGYC 1 cut(s) 73
HspAI GCGC 3 cut(s) 261, 458, 463
KroI GCCGGC 1 cut(s) 407
KroNI GCCGGC 1 cut(s) 409
Kzo9I GATC 2 cut(s) 240, 321
LpnPI CCDG 5 cut(s) 32, 177, 242, 288, 421
Lsp1109I GCAGC 2 cut(s) 51, 137
MalI GATC 2 cut(s) 242, 323
MboI GATC 2 cut(s) 240, 321
MboII GAAGA 8 cut(s) 25, 71, 162, 211, 221, 277, 304, 371
MflI RGATCY 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 4 cut(s) 176, 329, 398, 450
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 2 cut(s) 161, 265
MnlI CCTC 9 cut(s) 3, 54, 86, 100, 106, 117, 127, 292, 388
Mox20I TGGCCA 1 cut(s) 50
MroNI GCCGGC 1 cut(s) 407
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 50
MspI CCGG 1 cut(s) 408
MteI GCGCNGCGC 1 cut(s) 461
MvnI CGCG 4 cut(s) 261, 263, 458, 460
MwoI GCNNNNNNNGC 1 cut(s) 445
NaeI GCCGGC 1 cut(s) 409
NdeII GATC 2 cut(s) 240, 321
NgoMIV GCCGGC 1 cut(s) 407
NlaIV GGNNCC 3 cut(s) 162, 273, 406
NspV TTCGAA 2 cut(s) 173, 468
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PdiI GCCGGC 1 cut(s) 409
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 5 cut(s) 64, 221, 347, 367, 419
PkrI GCNGC 4 cut(s) 41, 152, 457, 462
Ple19I CGATCG 1 cut(s) 324
PleI GAGTC 2 cut(s) 161, 264
PpsI GAGTC 2 cut(s) 161, 264
PspN4I GGNNCC 3 cut(s) 162, 273, 406
PspPI GGNCC 3 cut(s) 160, 226, 405
PsuI RGATCY 1 cut(s) 240
PvuI CGATCG 1 cut(s) 324
SatI GCNGC 4 cut(s) 40, 151, 456, 461
Sau3AI GATC 2 cut(s) 240, 321
Sau96I GGNCC 3 cut(s) 160, 226, 405
SchI GAGTC 2 cut(s) 161, 265
SetI ASST 9 cut(s) 88, 98, 119, 152, 207, 255, 273, 303, 339
SfuI TTCGAA 2 cut(s) 173, 468
SinI GGWCC 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 4 cut(s) 176, 329, 398, 450
SsiI CCGC 3 cut(s) 344, 456, 460
TaaI ACNGT 1 cut(s) 55
TaqI TCGA 6 cut(s) 82, 131, 173, 207, 324, 468
TaqII GACCGA 1 cut(s) 214
TasI AATT 4 cut(s) 176, 329, 398, 450
TauI GCSGC 2 cut(s) 458, 463
TfiI GAWTC 5 cut(s) 64, 221, 347, 367, 419
TseI GCWGC 2 cut(s) 39, 150
TspGWI ACGGA 2 cut(s) 149, 420
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 2 cut(s) 176, 398
XmnI GAANNNNTTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.