pycom01g15360
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0022525
Physical Location & Seq
Forward (+)
15265839 .. 15266348
510 bp
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UTR
Exon/CDS
Intron
pycom01g15360.1

Sequence Viewer

Length: 510 bp
ATGAGATCGATCATTGGAGGTCCTTCTGACAAGCCAATGATTATCGTCACATACAAGGGTGTAGAGAAACAATTTGCTGCTGAAGAAATCTCATCTATGATTCTTGTGAAAATGCGGGAGATTGCTGAGGCCTACCTTGGTTCAACTGTGAAAAATTCAGTTATCACAGTCCCTGCTTACTTTAACGACTCTCAGCGACAGTCTACGAAAGAGGCAGGTGCTACTGCTGGCCTAAATGTGATGCGCATCATCAATGAGCCGACTGCTGCAGCCATTGCTTATGGGCTTGACAAGAAGGCCGGTTGGTATAGCAAGAGAAATGTGATGATATTTGATTTGGGCGGGGGCACTTTGGATGTTTCGCTACTTACAATAGCTGACGTTTGCTTTGATATTGATGAAAATGGCATCCTGCGTGTCTCTGCTGAGGACACATCCACCGGCCAAAAGAAGGGGCTCGCATTCAACTGTGACAGAAGAACTTGTGAAGGAATTGAGACATTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

170

Amino Acids

18.29

Weight (kDa)

5.27

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 7 - 126 4.1e-51 Hsp70 protein
MreB_Mbl PF06723 32 - 129 8.4e-09 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022525)

Species Orthologous Gene IDs
pyrus_communis pycom01g15360
rosa_laevigata RLG00000003667 RLG00000015323
rosa_samantha Rh7AG190400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
Acc16I TGCGCA 1 cut(s) 245
Acc36I ACCTGC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 203
AciI CCGC 2 cut(s) 115, 342
AcoI YGGCCR 1 cut(s) 442
AcsI RAATTY 1 cut(s) 154
AcuI CTGAAG 1 cut(s) 102
AfiI CCNNNNNNNGG 1 cut(s) 451
AgsI TTSAA 2 cut(s) 144, 466
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
Alw26I GTCTC 2 cut(s) 424, 491
AlwNI CAGNNNCTG 1 cut(s) 173
AoxI GGCC 4 cut(s) 129, 229, 297, 442
ApeKI GCWGC 3 cut(s) 77, 266, 269
ApoI RAATTY 1 cut(s) 154
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 1 cut(s) 20
AvaII GGWCC 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 459
BbvCI CCTCAGC 2 cut(s) 126, 426
BbvI GCAGC 3 cut(s) 64, 253, 281
BcoDI GTCTC 2 cut(s) 424, 491
BfmI CTRYAG 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 206
BisI GCNGC 3 cut(s) 78, 267, 270
BlsI GCNGC 3 cut(s) 79, 268, 271
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmsI GCATC 3 cut(s) 231, 255, 417
Bpu10I CCTNAGC 2 cut(s) 126, 426
Bsa29I ATCGAT 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse118I RCCGGY 2 cut(s) 299, 440
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 245
BseCI ATCGAT 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 3 cut(s) 361, 408, 434
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 3 cut(s) 117, 206, 417
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 3 cut(s) 64, 253, 281
BshFI GGCC 4 cut(s) 131, 231, 299, 444
BshVI ATCGAT 1 cut(s) 8
BsiSI CCGG 2 cut(s) 300, 441
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 451
BsmAI GTCTC 2 cut(s) 424, 491
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 4 cut(s) 131, 231, 299, 444
Bsp1286I GDGCHC 2 cut(s) 350, 459
Bsp143I GATC 2 cut(s) 5, 9
BspACI CCGC 2 cut(s) 115, 342
BspANI GGCC 4 cut(s) 131, 231, 299, 444
BspCNI CTCAG 3 cut(s) 118, 205, 418
BspDI ATCGAT 1 cut(s) 8
BspMAI CTGCAG 1 cut(s) 271
BspMI ACCTGC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 2 cut(s) 299, 440
BssAI RCCGGY 2 cut(s) 299, 440
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 2 cut(s) 5, 9
BssT1I CCWWGG 1 cut(s) 136
Bst4CI ACNGT 4 cut(s) 148, 169, 201, 470
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 229, 459
BstDEI CTNAG 3 cut(s) 126, 192, 426
BstF5I GGATG 3 cut(s) 361, 408, 434
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 2 cut(s) 8, 12
BstMAI GTCTC 2 cut(s) 424, 491
BstMBI GATC 2 cut(s) 5, 9
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 350
BstV1I GCAGC 3 cut(s) 64, 253, 281
Bsu15I ATCGAT 1 cut(s) 8
BsuRI GGCC 4 cut(s) 131, 231, 299, 444
BsuTUI ATCGAT 1 cut(s) 8
BtsCI GGATG 3 cut(s) 361, 408, 434
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 229, 459
CaiI CAGNNNCTG 1 cut(s) 173
CfoI GCGC 1 cut(s) 246
Cfr10I RCCGGY 2 cut(s) 299, 440
Cfr13I GGNCC 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 8
DdeI CTNAG 3 cut(s) 126, 192, 426
DpnI GATC 2 cut(s) 7, 11
DpnII GATC 2 cut(s) 5, 9
EaeI YGGCCR 1 cut(s) 442
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 136
FaiI YATR 4 cut(s) 52, 98, 282, 309
FaqI GGGAC 1 cut(s) 155
FauI CCCGC 2 cut(s) 108, 335
FblI GTMKAC 1 cut(s) 203
Fnu4HI GCNGC 3 cut(s) 78, 267, 270
FokI GGATG 3 cut(s) 368, 395, 421
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 3 cut(s) 78, 267, 270
FspAI RTGCGCAY 1 cut(s) 245
FspI TGCGCA 1 cut(s) 245
GlaI GCGC 1 cut(s) 245
GluI GCNGC 3 cut(s) 78, 267, 270
HaeIII GGCC 4 cut(s) 131, 231, 299, 444
HapII CCGG 2 cut(s) 300, 441
HhaI GCGC 1 cut(s) 246
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HinfI GANTC 2 cut(s) 100, 188
HpaII CCGG 2 cut(s) 300, 441
Hpy166II GTNNAC 1 cut(s) 204
Hpy188I TCNGA 1 cut(s) 28
Hpy8I GTNNAC 1 cut(s) 204
HpyAV CCTTC 4 cut(s) 33, 289, 445, 482
HpyCH4III ACNGT 4 cut(s) 148, 169, 201, 470
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 3 cut(s) 126, 192, 426
HpySE526I ACGT 1 cut(s) 381
HspAI GCGC 1 cut(s) 244
Kzo9I GATC 2 cut(s) 5, 9
LpnPI CCDG 6 cut(s) 186, 201, 213, 313, 425, 454
Lsp1109I GCAGC 3 cut(s) 64, 253, 281
LweI GCATC 3 cut(s) 231, 255, 417
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 2 cut(s) 46, 470
MalI GATC 2 cut(s) 7, 11
MboI GATC 2 cut(s) 5, 9
MboII GAAGA 2 cut(s) 95, 489
MhlI GDGCHC 2 cut(s) 350, 459
MluCI AATT 3 cut(s) 71, 154, 492
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 4 cut(s) 11, 121, 205, 421
MseI TTAA 2 cut(s) 183, 508
MspI CCGG 2 cut(s) 300, 441
Mva1269I GAATGC 1 cut(s) 461
MwoI GCNNNNNNNGC 1 cut(s) 275
NdeII GATC 2 cut(s) 5, 9
NmuCI GTSAC 2 cut(s) 46, 470
NsbI TGCGCA 1 cut(s) 245
PaqCI CACCTGC 1 cut(s) 206
PceI AGGCCT 1 cut(s) 131
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 3 cut(s) 79, 268, 271
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PpuMI RGGWCCY 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 20
PspPI GGNCC 1 cut(s) 20
PspPPI RGGWCCY 1 cut(s) 20
PstI CTGCAG 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 173
SaqAI TTAA 2 cut(s) 183, 508
SatI GCNGC 3 cut(s) 78, 267, 270
Sau3AI GATC 2 cut(s) 5, 9
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 182
SduI GDGCHC 2 cut(s) 350, 459
SetI ASST 5 cut(s) 22, 138, 220, 379, 384
SfaNI GCATC 3 cut(s) 231, 255, 417
SfcI CTRYAG 1 cut(s) 267
SinI GGWCC 1 cut(s) 20
Sse9I AATT 3 cut(s) 71, 154, 492
SseBI AGGCCT 1 cut(s) 131
SsiI CCGC 2 cut(s) 115, 342
StuI AGGCCT 1 cut(s) 131
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 4 cut(s) 148, 169, 201, 470
TaiI ACGT 1 cut(s) 384
TaqI TCGA 1 cut(s) 8
TasI AATT 3 cut(s) 71, 154, 492
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 2 cut(s) 183, 508
Tru9I TTAA 2 cut(s) 183, 508
TseFI GTSAC 2 cut(s) 46, 470
TseI GCWGC 3 cut(s) 77, 266, 269
Tsp45I GTSAC 2 cut(s) 46, 470
TspDTI ATGAA 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 20
XapI RAATTY 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.