RLG00000003667
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0022525
Physical Location & Seq
Forward (+)
51451394 .. 51451953
560 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003667

Sequence Viewer

Length: 483 bp
ATGTACCTGCATGCTGCATTAACTTATGCAAAGCGCTTGATTGGTAGGAGATTCAACGATGCTTCTGTCAAGAGTGATATGAAGTTTTGGCCTTTCAAAGTTCGTGGTGTCGACAATAAGCCGATGATTGTGGTCAACTACAAGAATGAAGAGAAGCGGTTTGCTGCTGAGGAGATATCATCCATGATTCTTGTTAAGATGAGGGAAATAGCAGAGGCTTATCTTGGATCGACAGTAAAGAATGCTGTTGTAACGGTCCCAGCATACTTTAATGATTTTCAACGTCAGGCTACAAAAGATGCTGGGATAATTGCGGGCATGAATGTTTTACGTATTATTAACGAGCCTACAGCTGCTGCAATTGCTTATGGCCTTGATAGAAAATACACCACTGTTGGAGGAAAGAACGTTCTTATTTTTGATCTGGGAGGTGGAACTTTTGATGTTCCCTTCTCAAAATTGAAGAGGGAATTTTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

161

Amino Acids

18.02

Weight (kDa)

9.78

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 7 - 155 2.3e-60 Hsp70 protein
MreB_Mbl PF06723 62 - 149 4e-07 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0022525)

Species Orthologous Gene IDs
pyrus_communis pycom01g15360
rosa_laevigata RLG00000003667 RLG00000015323
rosa_samantha Rh7AG190400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 111
AciI CCGC 2 cut(s) 157, 314
AclI AACGTT 1 cut(s) 408
AclWI GGATC 1 cut(s) 235
AcsI RAATTY 1 cut(s) 470
AfaI GTAC 1 cut(s) 5
AfeI AGCGCT 1 cut(s) 35
AgsI TTSAA 4 cut(s) 55, 97, 281, 463
AloI GAACNNNNNNTCC 2 cut(s) 393, 425
AluBI AGCT 1 cut(s) 353
AluI AGCT 1 cut(s) 353
AlwI GGATC 1 cut(s) 235
AlwNI CAGNNNCTG 1 cut(s) 356
Aor51HI AGCGCT 1 cut(s) 35
AoxI GGCC 2 cut(s) 89, 370
ApeKI GCWGC 4 cut(s) 14, 164, 353, 356
ApoI RAATTY 1 cut(s) 470
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 1 cut(s) 256
AvaII GGWCC 1 cut(s) 256
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 3 cut(s) 151, 340, 343
BfmI CTRYAG 1 cut(s) 348
BfoI RGCGCY 1 cut(s) 37
BfuAI ACCTGC 1 cut(s) 15
BisI GCNGC 4 cut(s) 15, 165, 354, 357
BlsI GCNGC 4 cut(s) 16, 166, 355, 358
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 1 cut(s) 258
BmsI GCATC 2 cut(s) 49, 289
Bpu10I CCTNAGC 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 332
BseGI GGATG 1 cut(s) 179
BseMII CTCAG 1 cut(s) 159
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 3 cut(s) 151, 340, 343
BseYI CCCAGC 2 cut(s) 259, 302
BshFI GGCC 2 cut(s) 91, 372
BslFI GGGAC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 2 cut(s) 91, 372
Bsp143I GATC 2 cut(s) 227, 421
BspACI CCGC 2 cut(s) 157, 314
BspANI GGCC 2 cut(s) 91, 372
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 258
BspMI ACCTGC 1 cut(s) 15
BspPI GGATC 1 cut(s) 235
BssMI GATC 2 cut(s) 227, 421
Bst4CI ACNGT 3 cut(s) 235, 256, 394
Bst6I CTCTTC 2 cut(s) 144, 458
BstBAI YACGTR 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 12, 316
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 179
BstH2I RGCGCY 1 cut(s) 37
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 2 cut(s) 230, 424
BstMBI GATC 2 cut(s) 227, 421
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 14
BstSFI CTRYAG 1 cut(s) 348
BstSNI TACGTA 1 cut(s) 332
BstV1I GCAGC 3 cut(s) 151, 340, 343
BsuRI GGCC 2 cut(s) 91, 372
BtsCI GGATG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 390
BveI ACCTGC 1 cut(s) 15
Cac8I GCNNGC 2 cut(s) 12, 316
CaiI CAGNNNCTG 1 cut(s) 356
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviAII CATG 3 cut(s) 11, 184, 319
CviJI RGCY 7 cut(s) 91, 121, 218, 290, 346, 353, 372
CviKI_1 RGCY 7 cut(s) 91, 121, 218, 290, 346, 353, 372
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 168
DpnI GATC 2 cut(s) 229, 423
DpnII GATC 2 cut(s) 227, 421
Eam1104I CTCTTC 2 cut(s) 144, 458
EarI CTCTTC 2 cut(s) 144, 458
Eco105I TACGTA 1 cut(s) 332
Eco32I GATATC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 256
Eco47III AGCGCT 1 cut(s) 35
EcoRV GATATC 1 cut(s) 177
FaeI CATG 3 cut(s) 14, 187, 322
FaiI YATR 7 cut(s) 12, 27, 80, 185, 265, 320, 369
FaqI GGGAC 1 cut(s) 242
FatI CATG 3 cut(s) 10, 183, 318
FauI CCCGC 1 cut(s) 307
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 4 cut(s) 15, 165, 354, 357
FokI GGATG 1 cut(s) 166
Fsp4HI GCNGC 4 cut(s) 15, 165, 354, 357
GlaI GCGC 1 cut(s) 35
GluI GCNGC 4 cut(s) 15, 165, 354, 357
GsaI CCCAGC 2 cut(s) 263, 306
HaeII RGCGCY 1 cut(s) 37
HaeIII GGCC 2 cut(s) 91, 372
HhaI GCGC 1 cut(s) 36
Hin1II CATG 3 cut(s) 14, 187, 322
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HincII GTYRAC 2 cut(s) 112, 136
HindII GTYRAC 2 cut(s) 112, 136
HinfI GANTC 2 cut(s) 51, 187
Hpy166II GTNNAC 2 cut(s) 112, 136
Hpy188III TCNNGA 1 cut(s) 70
Hpy8I GTNNAC 2 cut(s) 112, 136
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 3 cut(s) 235, 256, 394
HpyCH4IV ACGT 3 cut(s) 283, 331, 408
HpyCH4V TGCA 4 cut(s) 10, 17, 29, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 3 cut(s) 283, 331, 408
Hsp92II CATG 3 cut(s) 14, 187, 322
HspAI GCGC 1 cut(s) 34
Kzo9I GATC 2 cut(s) 227, 421
LpnPI CCDG 5 cut(s) 20, 272, 273, 288, 410
Lsp1109I GCAGC 3 cut(s) 151, 340, 343
LweI GCATC 2 cut(s) 49, 289
MaeII ACGT 3 cut(s) 283, 331, 408
MaeIII GTNAC 1 cut(s) 250
MalI GATC 2 cut(s) 229, 423
MboI GATC 2 cut(s) 227, 421
MboII GAAGA 2 cut(s) 161, 475
MfeI CAATTG 1 cut(s) 360
MluCI AATT 4 cut(s) 309, 360, 458, 470
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 7 cut(s) 163, 195, 208, 392, 422, 459, 471
MseI TTAA 4 cut(s) 20, 195, 270, 339
MspA1I CMGCKG 1 cut(s) 353
MunI CAATTG 1 cut(s) 360
Mva1269I GAATGC 1 cut(s) 247
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeII GATC 2 cut(s) 227, 421
NlaIII CATG 3 cut(s) 14, 187, 322
NlaIV GGNNCC 1 cut(s) 258
NspI RCATGY 1 cut(s) 14
PaeI GCATGC 1 cut(s) 14
PctI GAATGC 1 cut(s) 247
PfeI GAWTC 2 cut(s) 51, 187
PkrI GCNGC 4 cut(s) 16, 166, 355, 358
Ppu21I YACGTR 1 cut(s) 332
Psp1406I AACGTT 1 cut(s) 408
PspFI CCCAGC 2 cut(s) 259, 302
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 256
PstNI CAGNNNCTG 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SalI GTCGAC 1 cut(s) 110
SaqAI TTAA 4 cut(s) 20, 195, 270, 339
SatI GCNGC 4 cut(s) 15, 165, 354, 357
Sau3AI GATC 2 cut(s) 227, 421
Sau96I GGNCC 1 cut(s) 256
SetI ASST 7 cut(s) 9, 286, 334, 355, 411, 433, 482
SfaNI GCATC 2 cut(s) 49, 289
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 1 cut(s) 256
SnaBI TACGTA 1 cut(s) 332
SphI GCATGC 1 cut(s) 14
Sse9I AATT 4 cut(s) 309, 360, 458, 470
SsiI CCGC 2 cut(s) 157, 314
TaaI ACNGT 3 cut(s) 235, 256, 394
TaiI ACGT 3 cut(s) 286, 334, 411
TaqI TCGA 2 cut(s) 111, 230
TasI AATT 4 cut(s) 309, 360, 458, 470
TfiI GAWTC 2 cut(s) 51, 187
Tru1I TTAA 4 cut(s) 20, 195, 270, 339
Tru9I TTAA 4 cut(s) 20, 195, 270, 339
TscAI CASTG 1 cut(s) 397
TseI GCWGC 4 cut(s) 14, 164, 353, 356
TspDTI ATGAA 3 cut(s) 95, 162, 335
TspRI CASTG 1 cut(s) 397
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 14
XmiI GTMKAC 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.