pycom01g18070

conserved protein UCP030365

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009181
Physical Location & Seq
Forward (+)
17203113 .. 17205181
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18070.2

Sequence Viewer

Length: 1413 bp
ATGGAGAATCGATGTGATTCGGGGAGAGTTTCCGACGGGGCAGTCCCGATGCATAGATTCGATTCAAAGATTGCTGGGGAGAAGCGAATCAGTACTGAGATGGGACGAGAACGTGAGCTGGGACCTCGCAAACGGGCGAAAATGCGAGATCTTAAGACGGTGTGCCGGTCCGAAGGAATTCAGACCGATAATCCAACATCGTTGAAAAACAAGGAATCGAGTGATCAATTTCAATCTGGTGATGATGAGATGTCTCAAGTTACTGAGGTGCCTATAACTTTGGGTGTAGATGCTTCCCAAGCTGGAAATTCTTGGAGAAATACATTGTCAGTACCAGTCAATCATGCTTCTAGACTAGATCTGACTACTGGTGCGTGCATTGGCAAAACTACGGTTCTAAACAACAGTCAGCAATGCACCGAGAGTTTTGAGATCACCTTGCTTAGGGATGAGAGTAAATGTGAAAAGGCGAAAGGCATTGGATTGGATCTCAATGCAGCAGATGTTTCTACTTCAGAAAAACAAGATCCATTTTATCCTTATAAAAGCACCAATTCCTTGAAGCCAAGAGCTGCCTCTGATTGTGGGAGTTGCACTGGCCCACTGGAAGAGAAAGATCCAATGAAAGTATGGAAGGAGATGAAGCAAAATGGTTTCCTATCATCCACTCATGGAGGCATACCGGTGCCAAAACCACATCCCAAGAAGACTAAAAATGACGAGGTCAAGAAAAAGATGGAACGCGCAAAGAGGGAACAAGTGGACAGGTTCGCGAAGGTTGCTGCTCCAAGTGGACTGCTCAATGAACTGAACCCTGGGATTATCAACCATGTCAGGAACAGAAAACAGGTCCGTTCAATAATAGAGTCCCTTGTAAAATTAGAAAGACTTGAAAATGACCGCCTGGGAAACAAGCAAACAACCCATCCGAAGAGTGGAACTTTTGAAATTGGGAACAGGCAGGACATTGGAAATATGAATGACTCTGCAATTCATCTCTATCATGAAAATCGGCCTCCAAATACCGTCTGTCATGACTGGCAGACAAGAGGGCTGTCTATATTGATGAACAAATCTTCTTTGATTCCAGTAGACAAAGGTGGAGATGTTGAACGAACCACAGCAAAGAGGTTTAGTGGTAAAAGTCTTGAATCGCACTCCATCCCTGAAAGAGAGGAAGATTCGCTAGCGTTGAAGTTGTCATCATCAATGCAGGCGCCCGAGGATGACAGCCCTTTGTCTAGTGAGGAAACATCATCCTATCTCTCTATCAAAGCTGCTACCATTGCTTCTCAATGGTTGGAACTTATTCTTCTGGACGTCAAAGGACGTCTTGAAGGTAATGCTCTATCTCTTATATCACTAGGGGATCTCGTTGATGGAGCATATTCTTATCTGTTCTTTATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

471

Amino Acids

52.03

Weight (kDa)

7.59

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
AasI GACNNNNNNGTC 1 cut(s) 41
AatII GACGTC 2 cut(s) 1323, 1333
AccB1I GGYRCC 3 cut(s) 268, 685, 1216
AccI GTMKAC 1 cut(s) 1092
AccII CGCG 2 cut(s) 744, 773
AciI CCGC 1 cut(s) 901
AclWI GGATC 4 cut(s) 495, 521, 611, 1377
AcsI RAATTY 2 cut(s) 177, 307
AcuI CTGAAG 1 cut(s) 498
AcyI GRCGYC 3 cut(s) 1217, 1320, 1330
AfaI GTAC 2 cut(s) 94, 333
AfiI CCNNNNNNNGG 2 cut(s) 444, 935
AflII CTTAAG 1 cut(s) 152
AgeI ACCGGT 1 cut(s) 682
AjnI CCWGG 2 cut(s) 814, 903
AluBI AGCT 4 cut(s) 118, 302, 572, 1277
AluI AGCT 4 cut(s) 118, 302, 572, 1277
Alw26I GTCTC 1 cut(s) 258
AlwI GGATC 4 cut(s) 495, 521, 611, 1377
Ama87I CYCGRG 1 cut(s) 1220
AoxI GGCC 2 cut(s) 598, 1013
ApeKI GCWGC 4 cut(s) 497, 572, 782, 1277
ApoI RAATTY 2 cut(s) 177, 307
AsiGI ACCGGT 1 cut(s) 682
Asp700I GAANNNNTTC 2 cut(s) 177, 1308
AspLEI GCGC 2 cut(s) 746, 1219
AspS9I GGNCC 4 cut(s) 122, 168, 599, 850
AsuHPI GGTGA 2 cut(s) 251, 427
AsuNHI GCTAGC 1 cut(s) 1186
AvaI CYCGRG 1 cut(s) 1220
AvaII GGWCC 3 cut(s) 122, 168, 850
BanI GGYRCC 3 cut(s) 268, 685, 1216
BbsI GAAGAC 1 cut(s) 713
BbvI GCAGC 4 cut(s) 509, 559, 769, 1264
BccI CCATC 5 cut(s) 94, 730, 933, 1169, 1373
BciT130I CCWGG 2 cut(s) 816, 905
BclI TGATCA 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 5 cut(s) 351, 356, 1187, 1242, 1364
BfoI RGCGCY 1 cut(s) 1220
BfrI CTTAAG 1 cut(s) 152
BglII AGATCT 2 cut(s) 148, 358
BisI GCNGC 4 cut(s) 498, 573, 783, 1278
BlsI GCNGC 4 cut(s) 499, 574, 784, 1279
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 2 cut(s) 816, 905
Bme18I GGWCC 3 cut(s) 122, 168, 850
BmeT110I CYCGRG 1 cut(s) 1220
BmgT120I GGNCC 4 cut(s) 122, 168, 599, 850
BmiI GGNNCC 4 cut(s) 123, 270, 687, 1218
BmrFI CCNGG 2 cut(s) 816, 905
BmsI GCATC 2 cut(s) 39, 280
BmtI GCTAGC 1 cut(s) 1190
BpiI GAAGAC 1 cut(s) 713
Bpu10I CCTNAGC 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 10
BsaHI GRCGYC 3 cut(s) 1217, 1320, 1330
BsaJI CCNNGG 4 cut(s) 814, 815, 904, 1221
BsaWI WCCGGW 1 cut(s) 682
Bsc4I CCNNNNNNNGG 2 cut(s) 444, 935
Bse118I RCCGGY 2 cut(s) 165, 682
Bse1I ACTGG 6 cut(s) 335, 373, 601, 609, 1043, 1088
Bse3DI GCAATG 2 cut(s) 419, 1284
BseBI CCWGG 2 cut(s) 816, 905
BseCI ATCGAT 1 cut(s) 10
BseDI CCNNGG 4 cut(s) 814, 815, 904, 1221
BseGI GGATG 7 cut(s) 454, 662, 697, 925, 1161, 1231, 1256
BseLI CCNNNNNNNGG 2 cut(s) 444, 935
BseMI GCAATG 2 cut(s) 419, 1284
BseMII CTCAG 2 cut(s) 87, 255
BseNI ACTGG 6 cut(s) 335, 373, 601, 609, 1043, 1088
BseXI GCAGC 4 cut(s) 509, 559, 769, 1264
BseYI CCCAGC 2 cut(s) 74, 118
Bsh1236I CGCG 2 cut(s) 744, 773
BshFI GGCC 2 cut(s) 600, 1015
BshNI GGYRCC 3 cut(s) 268, 685, 1216
BshTI ACCGGT 1 cut(s) 682
BshVI ATCGAT 1 cut(s) 10
BsiHKCI CYCGRG 1 cut(s) 1220
BsiSI CCGG 2 cut(s) 166, 683
BslFI GGGAC 4 cut(s) 29, 117, 135, 853
BslI CCNNNNNNNGG 2 cut(s) 444, 935
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 4 cut(s) 29, 117, 135, 853
BsnI GGCC 2 cut(s) 600, 1015
BsoBI CYCGRG 1 cut(s) 1220
Bsp143I GATC 8 cut(s) 148, 223, 358, 432, 487, 526, 616, 1369
Bsp68I TCGCGA 1 cut(s) 773
BspACI CCGC 1 cut(s) 901
BspANI GGCC 2 cut(s) 600, 1015
BspCNI CTCAG 2 cut(s) 88, 256
BspDI ATCGAT 1 cut(s) 10
BspFNI CGCG 2 cut(s) 744, 773
BspHI TCATGA 2 cut(s) 1003, 1033
BspLI GGNNCC 4 cut(s) 123, 270, 687, 1218
BspOI GCTAGC 1 cut(s) 1190
BspPI GGATC 4 cut(s) 495, 521, 611, 1377
BspT107I GGYRCC 3 cut(s) 268, 685, 1216
BspTI CTTAAG 1 cut(s) 152
BsrDI GCAATG 2 cut(s) 419, 1284
BsrFI RCCGGY 2 cut(s) 165, 682
BsrI ACTGG 6 cut(s) 335, 373, 601, 609, 1043, 1088
BssAI RCCGGY 2 cut(s) 165, 682
BssECI CCNNGG 4 cut(s) 814, 815, 904, 1221
BssMI GATC 8 cut(s) 148, 223, 358, 432, 487, 526, 616, 1369
BssNI GRCGYC 3 cut(s) 1217, 1320, 1330
Bst2UI CCWGG 2 cut(s) 816, 905
Bst4CI ACNGT 4 cut(s) 160, 394, 407, 1027
Bst6I CTCTTC 2 cut(s) 603, 926
BstACI GRCGYC 3 cut(s) 1217, 1320, 1330
BstAFI CTTAAG 1 cut(s) 152
BstC8I GCNNGC 3 cut(s) 376, 1188, 1215
BstDEI CTNAG 3 cut(s) 96, 264, 443
BstENI CCTNNNNNAGG 1 cut(s) 442
BstF5I GGATG 7 cut(s) 454, 662, 697, 925, 1161, 1231, 1256
BstFNI CGCG 2 cut(s) 744, 773
BstH2I RGCGCY 1 cut(s) 1220
BstHHI GCGC 2 cut(s) 746, 1219
BstKTI GATC 8 cut(s) 151, 226, 361, 435, 490, 529, 619, 1372
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 8 cut(s) 148, 223, 358, 432, 487, 526, 616, 1369
BstMWI GCNNNNNNNGC 3 cut(s) 299, 779, 1286
BstNI CCWGG 2 cut(s) 816, 905
BstSCI CCNGG 2 cut(s) 814, 903
BstUI CGCG 2 cut(s) 744, 773
BstV1I GCAGC 4 cut(s) 509, 559, 769, 1264
BstV2I GAAGAC 1 cut(s) 713
BstX2I RGATCY 6 cut(s) 148, 358, 487, 526, 616, 1369
BstYI RGATCY 6 cut(s) 148, 358, 487, 526, 616, 1369
Bsu15I ATCGAT 1 cut(s) 10
BsuRI GGCC 2 cut(s) 600, 1015
BsuTUI ATCGAT 1 cut(s) 10
BtsCI GGATG 7 cut(s) 454, 662, 697, 925, 1161, 1231, 1256
BtsIMutI CAGTG 2 cut(s) 594, 602
BtuMI TCGCGA 1 cut(s) 773
Cac8I GCNNGC 3 cut(s) 376, 1188, 1215
CciI TCATGA 2 cut(s) 1003, 1033
CfoI GCGC 2 cut(s) 746, 1219
Cfr10I RCCGGY 2 cut(s) 165, 682
Cfr13I GGNCC 4 cut(s) 122, 168, 599, 850
ClaI ATCGAT 1 cut(s) 10
CpoI CGGWCCG 1 cut(s) 168
Csp6I GTAC 2 cut(s) 93, 332
CspAI ACCGGT 1 cut(s) 682
CspI CGGWCCG 1 cut(s) 168
CviAII CATG 5 cut(s) 344, 671, 830, 1004, 1034
CviJI RGCY 9 cut(s) 118, 302, 565, 572, 600, 1015, 1054, 1233, 1277
CviKI_1 RGCY 9 cut(s) 118, 302, 565, 572, 600, 1015, 1054, 1233, 1277
CviQI GTAC 2 cut(s) 93, 332
DdeI CTNAG 3 cut(s) 96, 264, 443
DinI GGCGCC 1 cut(s) 1218
DpnI GATC 8 cut(s) 150, 225, 360, 434, 489, 528, 618, 1371
DpnII GATC 8 cut(s) 148, 223, 358, 432, 487, 526, 616, 1369
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
Eam1104I CTCTTC 2 cut(s) 603, 926
EarI CTCTTC 2 cut(s) 603, 926
Eco47I GGWCC 3 cut(s) 122, 168, 850
Eco57I CTGAAG 1 cut(s) 498
Eco88I CYCGRG 1 cut(s) 1220
EcoNI CCTNNNNNAGG 1 cut(s) 442
EcoO109I RGGNCCY 1 cut(s) 122
EcoRI GAATTC 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 814, 903
EcoT22I ATGCAT 1 cut(s) 54
EgeI GGCGCC 1 cut(s) 1218
EheI GGCGCC 1 cut(s) 1218
FaeI CATG 5 cut(s) 347, 674, 833, 1007, 1037
FalI AAGNNNNNCTT 2 cut(s) 1317, 1349
FaqI GGGAC 4 cut(s) 29, 117, 135, 853
FatI CATG 5 cut(s) 343, 670, 829, 1003, 1033
FbaI TGATCA 1 cut(s) 223
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 4 cut(s) 498, 573, 783, 1278
FokI GGATG 7 cut(s) 461, 649, 684, 912, 1148, 1238, 1243
Fsp4HI GCNGC 4 cut(s) 498, 573, 783, 1278
FspBI CTAG 5 cut(s) 351, 356, 1187, 1242, 1364
GlaI GCGC 2 cut(s) 745, 1218
GluI GCNGC 4 cut(s) 498, 573, 783, 1278
GsaI CCCAGC 2 cut(s) 78, 122
HaeII RGCGCY 1 cut(s) 1220
HaeIII GGCC 2 cut(s) 600, 1015
HapII CCGG 2 cut(s) 166, 683
HhaI GCGC 2 cut(s) 746, 1219
Hin1I GRCGYC 3 cut(s) 1217, 1320, 1330
Hin1II CATG 5 cut(s) 347, 674, 833, 1007, 1037
Hin6I GCGC 2 cut(s) 744, 1217
HinP1I GCGC 2 cut(s) 744, 1217
HpaII CCGG 2 cut(s) 166, 683
HphI GGTGA 2 cut(s) 251, 427
Hpy166II GTNNAC 3 cut(s) 763, 794, 1093
Hpy188I TCNGA 7 cut(s) 34, 172, 183, 363, 517, 580, 930
Hpy8I GTNNAC 3 cut(s) 763, 794, 1093
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 4 cut(s) 167, 628, 769, 1331
HpyCH4III ACNGT 4 cut(s) 160, 394, 407, 1027
HpyCH4IV ACGT 3 cut(s) 112, 1320, 1330
HpyCH4V TGCA 7 cut(s) 52, 378, 417, 497, 594, 989, 1213
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 779, 1286
HpyF3I CTNAG 3 cut(s) 96, 264, 443
HpySE526I ACGT 3 cut(s) 112, 1320, 1330
Hsp92I GRCGYC 3 cut(s) 1217, 1320, 1330
Hsp92II CATG 5 cut(s) 347, 674, 833, 1007, 1037
HspAI GCGC 2 cut(s) 744, 1217
KasI GGCGCC 1 cut(s) 1216
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 8 cut(s) 148, 223, 358, 432, 487, 526, 616, 1369
LmnI GCTCC 2 cut(s) 790, 1382
Lsp1109I GCAGC 4 cut(s) 509, 559, 769, 1264
LweI GCATC 2 cut(s) 39, 280
MaeI CTAG 5 cut(s) 351, 356, 1187, 1242, 1364
MaeII ACGT 3 cut(s) 112, 1320, 1330
MaeIII GTNAC 1 cut(s) 259
MalI GATC 8 cut(s) 150, 225, 360, 434, 489, 528, 618, 1371
MboI GATC 8 cut(s) 148, 223, 358, 432, 487, 526, 616, 1369
MboII GAAGA 6 cut(s) 620, 718, 943, 1068, 1190, 1304
MflI RGATCY 6 cut(s) 148, 358, 487, 526, 616, 1369
MluCI AATT 7 cut(s) 177, 227, 307, 553, 878, 948, 990
Mly113I GGCGCC 1 cut(s) 1217
MlyI GAGTC 2 cut(s) 875, 977
MmeI TCCRAC 3 cut(s) 57, 218, 1281
Mph1103I ATGCAT 1 cut(s) 54
MroXI GAANNNNTTC 2 cut(s) 177, 1308
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 683
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 2 cut(s) 166, 683
MspR9I CCNGG 2 cut(s) 816, 905
MvaI CCWGG 2 cut(s) 816, 905
MvnI CGCG 2 cut(s) 744, 773
MwoI GCNNNNNNNGC 3 cut(s) 299, 779, 1286
NarI GGCGCC 1 cut(s) 1217
NdeII GATC 8 cut(s) 148, 223, 358, 432, 487, 526, 616, 1369
NheI GCTAGC 1 cut(s) 1186
NlaIII CATG 5 cut(s) 347, 674, 833, 1007, 1037
NlaIV GGNNCC 4 cut(s) 123, 270, 687, 1218
NruI TCGCGA 1 cut(s) 773
NsiI ATGCAT 1 cut(s) 54
PagI TCATGA 2 cut(s) 1003, 1033
PasI CCCWGGG 1 cut(s) 815
PdmI GAANNNNTTC 2 cut(s) 177, 1308
PfeI GAWTC 9 cut(s) 7, 17, 57, 62, 87, 215, 1084, 1151, 1181
PflFI GACNNNGTC 1 cut(s) 722
PinAI ACCGGT 1 cut(s) 682
PkrI GCNGC 4 cut(s) 499, 574, 784, 1279
PleI GAGTC 2 cut(s) 874, 977
PluTI GGCGCC 1 cut(s) 1220
PpsI GAGTC 2 cut(s) 874, 977
PpuMI RGGWCCY 1 cut(s) 122
PsiI TTATAA 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 814, 903
PspFI CCCAGC 2 cut(s) 74, 118
PspGI CCWGG 2 cut(s) 814, 903
PspN4I GGNNCC 4 cut(s) 123, 270, 687, 1218
PspPI GGNCC 4 cut(s) 122, 168, 599, 850
PspPPI RGGWCCY 1 cut(s) 122
PsuI RGATCY 6 cut(s) 148, 358, 487, 526, 616, 1369
PsyI GACNNNGTC 1 cut(s) 722
RruI TCGCGA 1 cut(s) 773
RsaI GTAC 2 cut(s) 94, 333
RsaNI GTAC 2 cut(s) 93, 332
RseI CAYNNNNRTG 1 cut(s) 683
Rsr2I CGGWCCG 1 cut(s) 168
RsrII CGGWCCG 1 cut(s) 168
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 4 cut(s) 498, 573, 783, 1278
Sau3AI GATC 8 cut(s) 148, 223, 358, 432, 487, 526, 616, 1369
Sau96I GGNCC 4 cut(s) 122, 168, 599, 850
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 2 cut(s) 875, 977
ScrFI CCNGG 2 cut(s) 816, 905
SfaNI GCATC 2 cut(s) 39, 280
SfoI GGCGCC 1 cut(s) 1218
SinI GGWCC 3 cut(s) 122, 168, 850
SmiMI CAYNNNNRTG 1 cut(s) 683
SmlI CTYRAG 2 cut(s) 152, 255
SmoI CTYRAG 2 cut(s) 152, 255
Sse9I AATT 7 cut(s) 177, 227, 307, 553, 878, 948, 990
SsiI CCGC 1 cut(s) 901
SspDI GGCGCC 1 cut(s) 1216
SspMI CTAG 5 cut(s) 351, 356, 1187, 1242, 1364
StyD4I CCNGG 2 cut(s) 814, 903
TaaI ACNGT 4 cut(s) 160, 394, 407, 1027
TaiI ACGT 3 cut(s) 115, 1323, 1333
TaqI TCGA 3 cut(s) 10, 60, 218
TaqII GACCGA 1 cut(s) 200
TasI AATT 7 cut(s) 177, 227, 307, 553, 878, 948, 990
TatI WGTACW 1 cut(s) 92
TfiI GAWTC 9 cut(s) 7, 17, 57, 62, 87, 215, 1084, 1151, 1181
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 2 cut(s) 601, 609
TseI GCWGC 4 cut(s) 497, 572, 782, 1277
TspDTI ATGAA 7 cut(s) 638, 656, 819, 983, 992, 1020, 1082
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 2 cut(s) 601, 609
Tth111I GACNNNGTC 1 cut(s) 722
Vha464I CTTAAG 1 cut(s) 152
VpaK11BI GGWCC 3 cut(s) 122, 168, 850
XagI CCTNNNNNAGG 1 cut(s) 442
XapI RAATTY 2 cut(s) 177, 307
XbaI TCTAGA 1 cut(s) 350
XcmI CCANNNNNNNNNTGG 2 cut(s) 627, 932
XmiI GTMKAC 1 cut(s) 1092
XmnI GAANNNNTTC 2 cut(s) 177, 1308
XspI CTAG 5 cut(s) 351, 356, 1187, 1242, 1364
ZraI GACGTC 2 cut(s) 1321, 1331
ZrmI AGTACT 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.