pycom02g04420

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0022530
Physical Location & Seq
Reverse (-)
2889826 .. 2890628
803 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g04420.1

Sequence Viewer

Length: 483 bp
ATGAAATTCACCACCAACCCAGATTCTGAAGCGCAACTCAAGAGTCTTTTACCTCAAATAACTTCAGCATTTGGTGCAAATAAGTCGGTTCTTGAGGCAATTGATGAACTTAAAAAGCAGCAGCAGAGTTCAGATGCTGCTGGTAAGTTTGTCAATGCCATATGTCTATTGAGCGGCTTGTATGTTGCTGTCAAGTATTTTCTATCAAATTGTTTCTATTGCAGTGTCGCTAAAGTTGCATCTGCGCTTGGGAAAAATGACAATCAATTATCCCCATTAACTGGTGTGATGAAAACTGAATATTGCGTTGCTGTAAGATCGTCTCTGTGGATTGATTTTGCTGAAGGTGTGCGGGCAGTTTTGCTGGACAAGGATCAGAATCTGAAGTGGAACCCGTCAAAGTTGGAGGAGGTGAATCAGAGTGAAATGCAAGCTCTCTTCGAGCCATTGAGCCCGAACGGTGGGGAGTTGATGTGTGACTAA

Protein Analysis

161

Amino Acids

17.51

Weight (kDa)

5.04

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 7 - 147 8e-15 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022530)

Species Orthologous Gene IDs
pyrus_communis pycom02g04420 pycom02g04470 pycom15g17010
rosa_roxburghii Rroxscaffold_2G00148860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 174
AciI CCGC 2 cut(s) 174, 352
AclWI GGATC 1 cut(s) 381
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 4 cut(s) 48, 48, 363, 404
AfiI CCNNNNNNNGG 2 cut(s) 281, 461
AluBI AGCT 1 cut(s) 434
AluI AGCT 1 cut(s) 434
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 1 cut(s) 381
AlwNI CAGNNNCTG 3 cut(s) 26, 137, 382
ApeKI GCWGC 3 cut(s) 118, 121, 137
ApoI RAATTY 1 cut(s) 5
AspLEI GCGC 2 cut(s) 34, 247
AsuHPI GGTGA 1 cut(s) 424
BanII GRGCYC 1 cut(s) 455
BbvI GCAGC 3 cut(s) 124, 130, 133
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BcoDI GTCTC 1 cut(s) 327
BisI GCNGC 4 cut(s) 119, 122, 138, 175
BlsI GCNGC 4 cut(s) 120, 123, 139, 176
BmiI GGNNCC 1 cut(s) 392
BmsI GCATC 2 cut(s) 124, 248
BpuEI CTTGAG 2 cut(s) 23, 113
BsaBI GATNNNNATC 1 cut(s) 378
BsaXI ACNNNNNCTCC 1 cut(s) 458
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 461
Bse1I ACTGG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 378
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 2 cut(s) 281, 461
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 3 cut(s) 124, 130, 133
BslI CCNNNNNNNGG 2 cut(s) 281, 461
BsmAI GTCTC 1 cut(s) 327
BsmBI CGTCTC 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 455
Bsp143I GATC 2 cut(s) 317, 373
BspACI CCGC 2 cut(s) 174, 352
BspLI GGNNCC 1 cut(s) 392
BspPI GGATC 1 cut(s) 381
BsrBI CCGCTC 1 cut(s) 174
BsrI ACTGG 1 cut(s) 286
BssMI GATC 2 cut(s) 317, 373
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 354, 432
BstHHI GCGC 2 cut(s) 34, 247
BstKTI GATC 2 cut(s) 320, 376
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 2 cut(s) 317, 373
BstMWI GCNNNNNNNGC 2 cut(s) 74, 236
BstV1I GCAGC 3 cut(s) 124, 130, 133
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 2 cut(s) 354, 432
CaiI CAGNNNCTG 3 cut(s) 26, 137, 382
CfoI GCGC 2 cut(s) 34, 247
CviJI RGCY 4 cut(s) 177, 434, 445, 453
CviKI_1 RGCY 4 cut(s) 177, 434, 445, 453
DpnI GATC 2 cut(s) 319, 375
DpnII GATC 2 cut(s) 317, 373
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 455
Eco57I CTGAAG 4 cut(s) 48, 48, 363, 404
EcoT38I GRGCYC 1 cut(s) 455
Esp3I CGTCTC 1 cut(s) 327
FaiI YATR 3 cut(s) 161, 163, 183
FauI CCCGC 1 cut(s) 345
FauNDI CATATG 1 cut(s) 161
Fnu4HI GCNGC 4 cut(s) 119, 122, 138, 175
FriOI GRGCYC 1 cut(s) 455
Fsp4HI GCNGC 4 cut(s) 119, 122, 138, 175
GlaI GCGC 2 cut(s) 33, 246
GluI GCNGC 4 cut(s) 119, 122, 138, 175
HhaI GCGC 2 cut(s) 34, 247
Hin6I GCGC 2 cut(s) 32, 245
HinP1I GCGC 2 cut(s) 32, 245
HinfI GANTC 4 cut(s) 23, 43, 379, 415
HphI GGTGA 1 cut(s) 424
Hpy188I TCNGA 5 cut(s) 28, 133, 378, 384, 420
Hpy188III TCNNGA 2 cut(s) 40, 92
HpyAV CCTTC 1 cut(s) 338
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4V TGCA 4 cut(s) 77, 222, 239, 430
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 236
HspAI GCGC 2 cut(s) 32, 245
Kzo9I GATC 2 cut(s) 317, 373
LpnPI CCDG 4 cut(s) 33, 126, 267, 350
Lsp1109I GCAGC 3 cut(s) 124, 130, 133
LweI GCATC 2 cut(s) 124, 248
MaeIII GTNAC 1 cut(s) 476
MalI GATC 2 cut(s) 319, 375
MbiI CCGCTC 1 cut(s) 174
MboI GATC 2 cut(s) 317, 373
MboII GAAGA 1 cut(s) 430
MfeI CAATTG 1 cut(s) 99
MhlI GDGCHC 1 cut(s) 455
MluCI AATT 4 cut(s) 5, 99, 208, 266
MlyI GAGTC 1 cut(s) 52
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 4 cut(s) 63, 88, 400, 403
MseI TTAA 2 cut(s) 111, 278
MunI CAATTG 1 cut(s) 99
MwoI GCNNNNNNNGC 2 cut(s) 74, 236
NdeI CATATG 1 cut(s) 161
NdeII GATC 2 cut(s) 317, 373
NlaIV GGNNCC 1 cut(s) 392
NmuCI GTSAC 1 cut(s) 476
PfeI GAWTC 3 cut(s) 23, 379, 415
PflMI CCANNNNNTGG 1 cut(s) 281
PkrI GCNGC 4 cut(s) 120, 123, 139, 176
PleI GAGTC 1 cut(s) 51
PpsI GAGTC 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 392
PstNI CAGNNNCTG 3 cut(s) 26, 137, 382
SaqAI TTAA 2 cut(s) 111, 278
SatI GCNGC 4 cut(s) 119, 122, 138, 175
Sau3AI GATC 2 cut(s) 317, 373
SchI GAGTC 1 cut(s) 52
SduI GDGCHC 1 cut(s) 455
SetI ASST 4 cut(s) 55, 349, 414, 436
SfaNI GCATC 2 cut(s) 124, 248
SmlI CTYRAG 2 cut(s) 38, 92
SmoI CTYRAG 2 cut(s) 38, 92
Sse9I AATT 4 cut(s) 5, 99, 208, 266
SsiI CCGC 2 cut(s) 174, 352
SspI AATATT 1 cut(s) 302
TaaI ACNGT 1 cut(s) 461
TaqI TCGA 1 cut(s) 441
TasI AATT 4 cut(s) 5, 99, 208, 266
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 3 cut(s) 23, 379, 415
Tru1I TTAA 2 cut(s) 111, 278
Tru9I TTAA 2 cut(s) 111, 278
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 3 cut(s) 118, 121, 137
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 3 cut(s) 17, 120, 305
TspRI CASTG 1 cut(s) 229
Van91I CCANNNNNTGG 1 cut(s) 281
XapI RAATTY 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.