pycom02g04470

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0022530
Physical Location & Seq
Reverse (-)
2916027 .. 2917093
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g04470.2

Sequence Viewer

Length: 375 bp
ATGCAAAATCTCTTAGTACAAGAAGCCCATATAAGCTTCATGGATGGCATAACAGTGGGCTTTGGTATTGGCCTATCAGGTCACGGTCGCTACCGGATCATTACAGAGGATCCCCATCAGGACATTGAAACAACTGTGCTGAAATTCACCAGCAACCCAGATTCTGAAGCGCAACTCAAGACTCTTTTACCTCAAATAACTTCAGCATTTGGTGCAGATAAGTTGGTTCTCGAGACAATTGATGAACTTAAAAAGCAGCATCAGAGTTCAGATGCTACTGTTCGTCTTTACAGAATGATTTTGCTGAAGGTGTACGGCAGTTTTTGGTGGACAAGGATCAGGTTCGTTAATTATGCTGAAGATTCCTTACTATAA

Protein Analysis

125

Amino Acids

14.13

Weight (kDa)

5.74

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022530)

Species Orthologous Gene IDs
pyrus_communis pycom02g04420 pycom02g04470 pycom15g17010
rosa_roxburghii Rroxscaffold_2G00148860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 104, 104, 117, 344
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 3 cut(s) 186, 186, 326
AfaI GTAC 2 cut(s) 18, 314
AgsI TTSAA 1 cut(s) 128
AluBI AGCT 1 cut(s) 36
AluI AGCT 1 cut(s) 36
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 4 cut(s) 104, 104, 117, 344
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 1 cut(s) 256
ApoI RAATTY 1 cut(s) 143
AspLEI GCGC 1 cut(s) 172
AsuHPI GGTGA 1 cut(s) 139
AvaI CYCGRG 1 cut(s) 230
BamHI GGATCC 1 cut(s) 109
BarI GAAGNNNNNNTAC 1 cut(s) 351
BbvI GCAGC 1 cut(s) 268
BccI CCATC 2 cut(s) 38, 123
BceAI ACGGC 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 227
BisI GCNGC 1 cut(s) 257
BlsI GCNGC 1 cut(s) 258
BmeT110I CYCGRG 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 111
BmsI GCATC 2 cut(s) 262, 268
BpuEI CTTGAG 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 114
BsaWI WCCGGW 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 114
BseGI GGATG 1 cut(s) 49
BseJI GATNNNNATC 1 cut(s) 114
BseXI GCAGC 1 cut(s) 268
BsgI GTGCAG 1 cut(s) 234
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 227
BsnI GGCC 1 cut(s) 72
BsoBI CYCGRG 1 cut(s) 230
Bsp143I GATC 3 cut(s) 96, 109, 336
BspANI GGCC 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 111
BspPI GGATC 4 cut(s) 104, 104, 117, 344
BssMI GATC 3 cut(s) 96, 109, 336
Bst4CI ACNGT 4 cut(s) 55, 86, 136, 280
BstAPI GCANNNNNTGC 1 cut(s) 212
BstDEI CTNAG 1 cut(s) 13
BstF5I GGATG 1 cut(s) 49
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 3 cut(s) 99, 112, 339
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 3 cut(s) 96, 109, 336
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstV1I GCAGC 1 cut(s) 268
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 1 cut(s) 72
BtsCI GGATG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 60
CaiI CAGNNNCTG 1 cut(s) 164
CfoI GCGC 1 cut(s) 172
Csp6I GTAC 2 cut(s) 17, 313
CviAII CATG 1 cut(s) 40
CviJI RGCY 4 cut(s) 26, 36, 60, 72
CviKI_1 RGCY 4 cut(s) 26, 36, 60, 72
CviQI GTAC 2 cut(s) 17, 313
DdeI CTNAG 1 cut(s) 13
DpnI GATC 3 cut(s) 98, 111, 338
DpnII GATC 3 cut(s) 96, 109, 336
Eco57I CTGAAG 3 cut(s) 186, 186, 326
Eco88I CYCGRG 1 cut(s) 230
FaeI CATG 1 cut(s) 43
FaiI YATR 6 cut(s) 30, 32, 41, 50, 354, 373
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 1 cut(s) 257
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 1 cut(s) 257
GlaI GCGC 1 cut(s) 171
GluI GCNGC 1 cut(s) 257
HaeIII GGCC 1 cut(s) 72
HapII CCGG 1 cut(s) 94
HhaI GCGC 1 cut(s) 172
Hin1II CATG 1 cut(s) 43
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 3 cut(s) 161, 181, 362
HpaII CCGG 1 cut(s) 94
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 2 cut(s) 313, 330
Hpy188I TCNGA 3 cut(s) 166, 264, 271
Hpy188III TCNNGA 4 cut(s) 119, 178, 230, 232
Hpy8I GTNNAC 2 cut(s) 313, 330
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 4 cut(s) 55, 86, 136, 280
HpyCH4V TGCA 2 cut(s) 4, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 1 cut(s) 13
Hsp92II CATG 1 cut(s) 43
HspAI GCGC 1 cut(s) 170
Kzo9I GATC 3 cut(s) 96, 109, 336
LpnPI CCDG 6 cut(s) 63, 104, 107, 163, 171, 325
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 2 cut(s) 262, 268
MaeIII GTNAC 1 cut(s) 80
MalI GATC 3 cut(s) 98, 111, 338
MboI GATC 3 cut(s) 96, 109, 336
MboII GAAGA 1 cut(s) 371
MfeI CAATTG 1 cut(s) 237
MflI RGATCY 1 cut(s) 109
MluCI AATT 3 cut(s) 143, 237, 349
MlyI GAGTC 1 cut(s) 175
MnlI CCTC 2 cut(s) 100, 201
MseI TTAA 2 cut(s) 249, 348
MslI CAYNNNNRTG 1 cut(s) 53
MspI CCGG 1 cut(s) 94
MunI CAATTG 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 212
NdeII GATC 3 cut(s) 96, 109, 336
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 230
PfeI GAWTC 2 cut(s) 161, 362
PkrI GCNGC 1 cut(s) 258
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
PspN4I GGNNCC 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 2 cut(s) 18, 314
RsaNI GTAC 2 cut(s) 17, 313
RseI CAYNNNNRTG 1 cut(s) 53
SaqAI TTAA 2 cut(s) 249, 348
SatI GCNGC 1 cut(s) 257
Sau3AI GATC 3 cut(s) 96, 109, 336
SchI GAGTC 1 cut(s) 175
SetI ASST 5 cut(s) 38, 82, 193, 312, 344
SfaNI GCATC 2 cut(s) 262, 268
Sfr274I CTCGAG 1 cut(s) 230
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 53
SmlI CTYRAG 2 cut(s) 176, 230
SmoI CTYRAG 2 cut(s) 176, 230
Sse9I AATT 3 cut(s) 143, 237, 349
TaaI ACNGT 4 cut(s) 55, 86, 136, 280
TaqI TCGA 1 cut(s) 231
TasI AATT 3 cut(s) 143, 237, 349
TatI WGTACW 1 cut(s) 16
TfiI GAWTC 2 cut(s) 161, 362
Tru1I TTAA 2 cut(s) 249, 348
Tru9I TTAA 2 cut(s) 249, 348
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 28, 258
TspRI CASTG 1 cut(s) 60
XapI RAATTY 1 cut(s) 143
XhoI CTCGAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.