pycom02g04640

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0021252
Physical Location & Seq
Forward (+)
3023310 .. 3024300
991 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 261 bp
ATGTTCAGCTGCTTCACAATTGGTCAATGTGGCTCACATGGAGCTTCAAGAGATGGATTGAGCGAGAGTCGATTGATGGATCTCCTACATGGTGCTGAATATGTCCTCACCTATGAAGACAAGGATGGTGATTGGATGCTAGTTGGTGATGTTCCCTGGGAAATGTTCACCGACTCGTGTAAGAGGATGAGGATCATGAAGAGTTCAGAGGCTATTGGTCTAGCCCCAAGGGCCATGCAGAAGTGCAAAAATGGTAATTAG

Protein Analysis

87

Amino Acids

9.61

Weight (kDa)

5.56

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 1 - 77 6.2e-42 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021252)

Species Orthologous Gene IDs
pyrus_communis pycom02g04640
rosa_laevigata RLG00000017768
rosa_multiflora Rmu_co8491531.1_g000001
rosa_samantha Rh2AG406700 Rh2DG221000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 87, 200
AgsI TTSAA 1 cut(s) 48
AjnI CCWGG 1 cut(s) 155
AluBI AGCT 2 cut(s) 9, 44
AluI AGCT 2 cut(s) 9, 44
AlwI GGATC 2 cut(s) 87, 200
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 4 cut(s) 100, 140, 158, 160
BauI CACGAG 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 123
BccI CCATC 3 cut(s) 47, 70, 119
BciT130I CCWGG 1 cut(s) 157
BfaI CTAG 2 cut(s) 140, 221
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 1 cut(s) 10
BlsI GCNGC 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 126
BpiI GAAGAC 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 155, 156, 227
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 1 cut(s) 157
BseDI CCNNGG 3 cut(s) 155, 156, 227
BseGI GGATG 3 cut(s) 130, 141, 192
BseJI GATNNNNATC 1 cut(s) 191
BshFI GGCC 1 cut(s) 233
BsnI GGCC 1 cut(s) 233
Bsp143I GATC 2 cut(s) 79, 192
BspANI GGCC 1 cut(s) 233
BspHI TCATGA 1 cut(s) 195
BspPI GGATC 2 cut(s) 87, 200
BssECI CCNNGG 3 cut(s) 155, 156, 227
BssMI GATC 2 cut(s) 79, 192
BssSI CACGAG 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 175
Bst2UI CCWGG 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 194
BstF5I GGATG 3 cut(s) 130, 141, 192
BstKTI GATC 2 cut(s) 82, 195
BstMBI GATC 2 cut(s) 79, 192
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstNI CCWGG 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 155
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 79
BstYI RGATCY 1 cut(s) 79
BsuRI GGCC 1 cut(s) 233
BtsCI GGATG 3 cut(s) 130, 141, 192
CciI TCATGA 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 231
CviAII CATG 4 cut(s) 38, 89, 196, 235
CviJI RGCY 6 cut(s) 9, 33, 44, 212, 224, 233
CviKI_1 RGCY 6 cut(s) 9, 33, 44, 212, 224, 233
DpnI GATC 2 cut(s) 81, 194
DpnII GATC 2 cut(s) 79, 192
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 4 cut(s) 41, 92, 199, 238
FaiI YATR 6 cut(s) 39, 90, 102, 114, 197, 236
FatI CATG 4 cut(s) 37, 88, 195, 234
Fnu4HI GCNGC 1 cut(s) 10
FokI GGATG 3 cut(s) 137, 148, 199
Fsp4HI GCNGC 1 cut(s) 10
FspBI CTAG 2 cut(s) 140, 221
GluI GCNGC 1 cut(s) 10
HaeIII GGCC 1 cut(s) 233
Hin1II CATG 4 cut(s) 41, 92, 199, 238
HinfI GANTC 2 cut(s) 67, 173
HphI GGTGA 4 cut(s) 100, 140, 158, 160
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 2 cut(s) 48, 196
Hpy8I GTNNAC 1 cut(s) 168
HpyCH4V TGCA 2 cut(s) 238, 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
Hsp92II CATG 4 cut(s) 41, 92, 199, 238
Kzo9I GATC 2 cut(s) 79, 192
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 2 cut(s) 142, 169
LweI GCATC 1 cut(s) 126
MaeI CTAG 2 cut(s) 140, 221
MalI GATC 2 cut(s) 81, 194
MboI GATC 2 cut(s) 79, 192
MboII GAAGA 2 cut(s) 128, 211
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 1 cut(s) 79
MluCI AATT 2 cut(s) 18, 256
MlyI GAGTC 2 cut(s) 76, 167
MnlI CCTC 4 cut(s) 116, 177, 183, 202
MspA1I CMGCKG 1 cut(s) 9
MspR9I CCNGG 1 cut(s) 157
MunI CAATTG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 157
MwoI GCNNNNNNNGC 1 cut(s) 230
NdeII GATC 2 cut(s) 79, 192
NlaIII CATG 4 cut(s) 41, 92, 199, 238
PagI TCATGA 1 cut(s) 195
PasI CCCWGGG 1 cut(s) 156
PkrI GCNGC 1 cut(s) 11
PleI GAGTC 2 cut(s) 75, 167
PpsI GAGTC 2 cut(s) 75, 167
Psp6I CCWGG 1 cut(s) 155
PspGI CCWGG 1 cut(s) 155
PspPI GGNCC 1 cut(s) 231
PsuI RGATCY 1 cut(s) 79
PvuII CAGCTG 1 cut(s) 9
SatI GCNGC 1 cut(s) 10
Sau3AI GATC 2 cut(s) 79, 192
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 2 cut(s) 76, 167
ScrFI CCNGG 1 cut(s) 157
SetI ASST 3 cut(s) 11, 46, 113
SfaNI GCATC 1 cut(s) 126
Sse9I AATT 2 cut(s) 18, 256
SspMI CTAG 2 cut(s) 140, 221
StyD4I CCNGG 1 cut(s) 155
StyI CCWWGG 1 cut(s) 227
TaqI TCGA 1 cut(s) 70
TasI AATT 2 cut(s) 18, 256
TseI GCWGC 1 cut(s) 9
TspDTI ATGAA 2 cut(s) 129, 212
XspI CTAG 2 cut(s) 140, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.