RLG00000017768

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0021252
Physical Location & Seq
Reverse (-)
20971507 .. 20974389
2883 bp
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UTR
Exon/CDS
Intron
RLM00000017768

Sequence Viewer

Length: 321 bp
ATGGACATGATCCCCCAGAACAGCTCAGGACTGAAAGAACCTGGTTCTGATTCTGAATCACCAGAATCTGTCGGACAAGGGCAATATGGATCCCATGGAGCTCCAGGGAGGGAAAGATTGAGTGAGAGTAAGCTGAGAGACCTTCTTCATGGATCAGAATATGTGCTTACATATGAGGACAAGGATGGAGATTGGATGCTTGTCGGAGATGTCCCATGGGAGATGTTTATTGATTCATGCAAGAGGTTGAAGATAATGAAGGGCTCTGATGCCATTGGCTTAGCTCCTAGGTCCATGGAGAAATCCAAGAACCGGACCTAG

Protein Analysis

107

Amino Acids

11.73

Weight (kDa)

5.13

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 26 - 97 4.6e-38 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021252)

Species Orthologous Gene IDs
pyrus_communis pycom02g04640
rosa_laevigata RLG00000017768
rosa_multiflora Rmu_co8491531.1_g000001
rosa_samantha Rh2AG406700 Rh2DG221000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 4, 84, 97, 160
AfiI CCNNNNNNNGG 1 cut(s) 312
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 2 cut(s) 40, 103
AluBI AGCT 4 cut(s) 24, 101, 133, 284
AluI AGCT 4 cut(s) 24, 101, 133, 284
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 4 cut(s) 4, 84, 97, 160
AlwNI CAGNNNCTG 1 cut(s) 68
AspA2I CCTAGG 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 291, 315
AsuHPI GGTGA 1 cut(s) 51
AvaII GGWCC 2 cut(s) 291, 315
AvrII CCTAGG 1 cut(s) 287
BamHI GGATCC 1 cut(s) 89
BanII GRGCYC 2 cut(s) 103, 266
Bbv12I GWGCWC 1 cut(s) 103
BccI CCATC 1 cut(s) 179
BciT130I CCWGG 2 cut(s) 42, 105
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 2 cut(s) 288, 319
BlnI CCTAGG 1 cut(s) 287
BlpI GCTNAGC 1 cut(s) 280
Bme1390I CCNGG 2 cut(s) 42, 105
Bme18I GGWCC 2 cut(s) 291, 315
BmgT120I GGNCC 2 cut(s) 291, 315
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 2 cut(s) 42, 105
BmsI GCATC 2 cut(s) 186, 259
BpmI CTGGAG 1 cut(s) 87
Bpu10I CCTNAGC 1 cut(s) 25
Bpu1102I GCTNAGC 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 5 cut(s) 94, 104, 215, 287, 294
BsaWI WCCGGW 1 cut(s) 312
Bsc4I CCNNNNNNNGG 1 cut(s) 312
BseBI CCWGG 2 cut(s) 42, 105
BseDI CCNNGG 5 cut(s) 94, 104, 215, 287, 294
BseGI GGATG 2 cut(s) 190, 201
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMII CTCAG 2 cut(s) 39, 125
BsiHKAI GWGCWC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 313
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 312
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 197
Bso31I GGTCTC 1 cut(s) 132
Bsp1286I GDGCHC 2 cut(s) 103, 266
Bsp143I GATC 3 cut(s) 9, 89, 152
Bsp1720I GCTNAGC 1 cut(s) 280
Bsp19I CCATGG 3 cut(s) 94, 215, 294
BspCNI CTCAG 2 cut(s) 38, 126
BspLI GGNNCC 1 cut(s) 91
BspPI GGATC 4 cut(s) 4, 84, 97, 160
BspTNI GGTCTC 1 cut(s) 132
BssECI CCNNGG 5 cut(s) 94, 104, 215, 287, 294
BssMI GATC 3 cut(s) 9, 89, 152
BssT1I CCWWGG 4 cut(s) 94, 215, 287, 294
Bst2UI CCWGG 2 cut(s) 42, 105
BstDEI CTNAG 3 cut(s) 25, 134, 280
BstDSI CCRYGG 3 cut(s) 94, 215, 294
BstF5I GGATG 2 cut(s) 190, 201
BstKTI GATC 3 cut(s) 12, 92, 155
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 3 cut(s) 9, 89, 152
BstNI CCWGG 2 cut(s) 42, 105
BstSCI CCNGG 2 cut(s) 40, 103
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
BtgI CCRYGG 3 cut(s) 94, 215, 294
BtsCI GGATG 2 cut(s) 190, 201
CaiI CAGNNNCTG 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 291, 315
CsiI ACCWGGT 1 cut(s) 40
CviAII CATG 6 cut(s) 7, 95, 149, 216, 237, 295
CviJI RGCY 6 cut(s) 24, 101, 133, 264, 279, 284
CviKI_1 RGCY 6 cut(s) 24, 101, 133, 264, 279, 284
DdeI CTNAG 3 cut(s) 25, 134, 280
DpnI GATC 3 cut(s) 11, 91, 154
DpnII GATC 3 cut(s) 9, 89, 152
Ecl136II GAGCTC 1 cut(s) 101
Eco130I CCWWGG 4 cut(s) 94, 215, 287, 294
Eco24I GRGCYC 2 cut(s) 103, 266
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 291, 315
Eco53kI GAGCTC 1 cut(s) 101
EcoICRI GAGCTC 1 cut(s) 101
EcoRII CCWGG 2 cut(s) 40, 103
EcoT14I CCWWGG 4 cut(s) 94, 215, 287, 294
EcoT38I GRGCYC 2 cut(s) 103, 266
ErhI CCWWGG 4 cut(s) 94, 215, 287, 294
FaeI CATG 6 cut(s) 10, 98, 152, 219, 240, 298
FaqI GGGAC 1 cut(s) 197
FatI CATG 6 cut(s) 6, 94, 148, 215, 236, 294
FauNDI CATATG 1 cut(s) 172
FokI GGATG 2 cut(s) 197, 208
FriOI GRGCYC 2 cut(s) 103, 266
FspBI CTAG 2 cut(s) 288, 319
GsuI CTGGAG 1 cut(s) 87
HapII CCGG 1 cut(s) 313
Hin1II CATG 6 cut(s) 10, 98, 152, 219, 240, 298
HinfI GANTC 4 cut(s) 50, 56, 65, 233
HpaII CCGG 1 cut(s) 313
HphI GGTGA 1 cut(s) 51
Hpy188I TCNGA 6 cut(s) 49, 55, 74, 157, 206, 268
Hpy188III TCNNGA 1 cut(s) 27
HpyAV CCTTC 2 cut(s) 152, 253
HpyCH4V TGCA 1 cut(s) 240
HpyF3I CTNAG 3 cut(s) 25, 134, 280
Hsp92II CATG 6 cut(s) 10, 98, 152, 219, 240, 298
Kzo9I GATC 3 cut(s) 9, 89, 152
LmnI GCTCC 3 cut(s) 98, 106, 289
LpnPI CCDG 7 cut(s) 12, 27, 29, 54, 75, 90, 117
LweI GCATC 2 cut(s) 186, 259
MabI ACCWGGT 1 cut(s) 40
MaeI CTAG 2 cut(s) 288, 319
MalI GATC 3 cut(s) 11, 91, 154
MboI GATC 3 cut(s) 9, 89, 152
MboII GAAGA 2 cut(s) 137, 262
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 2 cut(s) 103, 266
MmeI TCCRAC 2 cut(s) 52, 184
MnlI CCTC 3 cut(s) 102, 169, 237
MspI CCGG 1 cut(s) 313
MspR9I CCNGG 2 cut(s) 42, 105
MvaI CCWGG 2 cut(s) 42, 105
NcoI CCATGG 3 cut(s) 94, 215, 294
NdeI CATATG 1 cut(s) 172
NdeII GATC 3 cut(s) 9, 89, 152
NlaIII CATG 6 cut(s) 10, 98, 152, 219, 240, 298
NlaIV GGNNCC 1 cut(s) 91
PfeI GAWTC 4 cut(s) 50, 56, 65, 233
Psp124BI GAGCTC 1 cut(s) 103
Psp6I CCWGG 2 cut(s) 40, 103
PspGI CCWGG 2 cut(s) 40, 103
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 2 cut(s) 291, 315
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 1 cut(s) 89
SacI GAGCTC 1 cut(s) 103
Sau3AI GATC 3 cut(s) 9, 89, 152
Sau96I GGNCC 2 cut(s) 291, 315
ScrFI CCNGG 2 cut(s) 42, 105
SduI GDGCHC 2 cut(s) 103, 266
SetI ASST 9 cut(s) 26, 43, 103, 135, 144, 248, 286, 293, 320
SexAI ACCWGGT 1 cut(s) 40
SfaNI GCATC 2 cut(s) 186, 259
SinI GGWCC 2 cut(s) 291, 315
SspMI CTAG 2 cut(s) 288, 319
SstI GAGCTC 1 cut(s) 103
StyD4I CCNGG 2 cut(s) 40, 103
StyI CCWWGG 4 cut(s) 94, 215, 287, 294
TfiI GAWTC 4 cut(s) 50, 56, 65, 233
TspDTI ATGAA 3 cut(s) 137, 225, 272
VpaK11BI GGWCC 2 cut(s) 291, 315
XmaJI CCTAGG 1 cut(s) 287
XspI CTAG 2 cut(s) 288, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.