Rh2DG221000

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0021252
Physical Location & Seq
Reverse (-)
21269806 .. 21272121
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG221000.1

Sequence Viewer

Length: 252 bp
ATGGCGGACTGGCAATATGGATCCCATGGAGCTCCAGGGAGGGAAAGATTGAGTGAGAGTAAGCTGAGAGACCTTCTTCATGGATCAGAATATGTGCTTACATATGAGGACAAGGATGGTGATTGGATGCTTGTCGGAGATGTCCCATGGGAGATGTTTATTGATTCATGCAAGAGGTTGAAGATAATGAAGGGCTCTGATGCCATTGGCTTAGCTCCTAGGTCCATGGAGAAATCCAAGAACCGGACCTAG

Protein Analysis

83

Amino Acids

9.49

Weight (kDa)

6.07

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 5 - 74 1.2e-37 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021252)

Species Orthologous Gene IDs
pyrus_communis pycom02g04640
rosa_laevigata RLG00000017768
rosa_multiflora Rmu_co8491531.1_g000001
rosa_samantha Rh2AG406700 Rh2DG221000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 5
AclWI GGATC 3 cut(s) 15, 28, 91
AfiI CCNNNNNNNGG 1 cut(s) 243
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 1 cut(s) 34
AluBI AGCT 3 cut(s) 32, 64, 215
AluI AGCT 3 cut(s) 32, 64, 215
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 3 cut(s) 15, 28, 91
AspA2I CCTAGG 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 222, 246
AsuHPI GGTGA 1 cut(s) 131
AvaII GGWCC 2 cut(s) 222, 246
AvrII CCTAGG 1 cut(s) 218
BamHI GGATCC 1 cut(s) 20
BanII GRGCYC 2 cut(s) 34, 197
Bbv12I GWGCWC 1 cut(s) 34
BccI CCATC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 2 cut(s) 219, 250
BlnI CCTAGG 1 cut(s) 218
BlpI GCTNAGC 1 cut(s) 211
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 2 cut(s) 222, 246
BmgT120I GGNCC 2 cut(s) 222, 246
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 2 cut(s) 117, 190
BpmI CTGGAG 1 cut(s) 18
Bpu1102I GCTNAGC 1 cut(s) 211
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 5 cut(s) 25, 35, 146, 218, 225
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 5 cut(s) 25, 35, 146, 218, 225
BseGI GGATG 2 cut(s) 121, 132
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 34
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 243
BsmAI GTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 63
Bsp1286I GDGCHC 2 cut(s) 34, 197
Bsp143I GATC 2 cut(s) 20, 83
Bsp1720I GCTNAGC 1 cut(s) 211
Bsp19I CCATGG 3 cut(s) 25, 146, 225
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 22
BspPI GGATC 3 cut(s) 15, 28, 91
BspTNI GGTCTC 1 cut(s) 63
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 5 cut(s) 25, 35, 146, 218, 225
BssMI GATC 2 cut(s) 20, 83
BssT1I CCWWGG 4 cut(s) 25, 146, 218, 225
Bst2UI CCWGG 1 cut(s) 36
BstDEI CTNAG 2 cut(s) 65, 211
BstDSI CCRYGG 3 cut(s) 25, 146, 225
BstF5I GGATG 2 cut(s) 121, 132
BstKTI GATC 2 cut(s) 23, 86
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 2 cut(s) 20, 83
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
BtgI CCRYGG 3 cut(s) 25, 146, 225
BtsCI GGATG 2 cut(s) 121, 132
Cfr13I GGNCC 2 cut(s) 222, 246
CviAII CATG 5 cut(s) 26, 80, 147, 168, 226
CviJI RGCY 5 cut(s) 32, 64, 195, 210, 215
CviKI_1 RGCY 5 cut(s) 32, 64, 195, 210, 215
DdeI CTNAG 2 cut(s) 65, 211
DpnI GATC 2 cut(s) 22, 85
DpnII GATC 2 cut(s) 20, 83
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 32
Eco130I CCWWGG 4 cut(s) 25, 146, 218, 225
Eco24I GRGCYC 2 cut(s) 34, 197
Eco31I GGTCTC 1 cut(s) 63
Eco47I GGWCC 2 cut(s) 222, 246
Eco53kI GAGCTC 1 cut(s) 32
EcoICRI GAGCTC 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 4 cut(s) 25, 146, 218, 225
EcoT38I GRGCYC 2 cut(s) 34, 197
ErhI CCWWGG 4 cut(s) 25, 146, 218, 225
FaeI CATG 5 cut(s) 29, 83, 150, 171, 229
FaiI YATR 9 cut(s) 18, 27, 81, 93, 103, 105, 148, 169, 227
FaqI GGGAC 1 cut(s) 128
FatI CATG 5 cut(s) 25, 79, 146, 167, 225
FauNDI CATATG 1 cut(s) 103
FokI GGATG 2 cut(s) 128, 139
FriOI GRGCYC 2 cut(s) 34, 197
FspBI CTAG 2 cut(s) 219, 250
GsuI CTGGAG 1 cut(s) 18
HapII CCGG 1 cut(s) 244
Hin1II CATG 5 cut(s) 29, 83, 150, 171, 229
HinfI GANTC 1 cut(s) 164
HpaII CCGG 1 cut(s) 244
HphI GGTGA 1 cut(s) 131
Hpy188I TCNGA 3 cut(s) 88, 137, 199
HpyAV CCTTC 2 cut(s) 83, 184
HpyCH4V TGCA 1 cut(s) 171
HpyF3I CTNAG 2 cut(s) 65, 211
Hsp92II CATG 5 cut(s) 29, 83, 150, 171, 229
Kzo9I GATC 2 cut(s) 20, 83
LmnI GCTCC 3 cut(s) 29, 37, 220
LpnPI CCDG 2 cut(s) 21, 48
LweI GCATC 2 cut(s) 117, 190
MaeI CTAG 2 cut(s) 219, 250
MalI GATC 2 cut(s) 22, 85
MboI GATC 2 cut(s) 20, 83
MboII GAAGA 2 cut(s) 68, 193
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 2 cut(s) 34, 197
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 3 cut(s) 33, 100, 168
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 36
NcoI CCATGG 3 cut(s) 25, 146, 225
NdeI CATATG 1 cut(s) 103
NdeII GATC 2 cut(s) 20, 83
NlaIII CATG 5 cut(s) 29, 83, 150, 171, 229
NlaIV GGNNCC 1 cut(s) 22
PfeI GAWTC 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 222, 246
PsuI RGATCY 1 cut(s) 20
SacI GAGCTC 1 cut(s) 34
Sau3AI GATC 2 cut(s) 20, 83
Sau96I GGNCC 2 cut(s) 222, 246
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 2 cut(s) 34, 197
SetI ASST 7 cut(s) 34, 66, 75, 179, 217, 224, 251
SfaNI GCATC 2 cut(s) 117, 190
SinI GGWCC 2 cut(s) 222, 246
SsiI CCGC 1 cut(s) 5
SspMI CTAG 2 cut(s) 219, 250
SstI GAGCTC 1 cut(s) 34
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 4 cut(s) 25, 146, 218, 225
TfiI GAWTC 1 cut(s) 164
TspDTI ATGAA 3 cut(s) 68, 156, 203
VpaK11BI GGWCC 2 cut(s) 222, 246
XmaJI CCTAGG 1 cut(s) 218
XspI CTAG 2 cut(s) 219, 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.