pycom03g05710

nudC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013389
Physical Location & Seq
Reverse (-)
4529858 .. 4531331
1474 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g05710.1

Sequence Viewer

Length: 477 bp
ATGGCCGAGAAATTGGCACCGGAGAAGCGCCATAGCTTCGTTCACAATGGTCAGAAAGTGTTTGAATGGGATCAAACCCTAGAGGAGGTAAATATGTACATAAGTTTACCCCCAAATGTTCCTTCAAAGCAATTTTTCTGCAAAATTCAGTCGAAGCACGTTGAAGTCGGCATCAAAGGCAATCCTCCGTACCTCAATCATGACCTTACTTGCCCGGTGAAGACGGACTGTTCTTTCTGGACACTAGAGGATGATATAATGCACATAACACTGCAAAAGAGGGACAAAGGTCAGACATGGGCATCACCAATAGTGGGCGAGGGTCAGCTCGATCGATACTCCATGGATCTTGAACAAAAGCGCCTTATGCTTCAGAGATTTCAAGAAGAGAACCCAGGGTTTGATTTCTCACAAGCCCAGTTCACTGGAGGCTGCCCTGATCGATCCAAGGACCTTTATGGGCGGTATCCGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CS

Protein Analysis

159

Amino Acids

18.37

Weight (kDa)

5.94

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 21 - 93 2.1e-19 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013389)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58740
fragaria_vesca FvH4_3g38540
malus_domestica MD03G1070800.v1.1 MD11G1074800.v1.1
prunus_persica Prupe.6G057600_v2.0.a1 Prupe.6G057600_v2.0.a1
pyrus_communis pycom03g05710
rosa_chinensis RchiOBHm_Chr5g0069441
rosa_laevigata RLG00000036053
rosa_multiflora Rmu_sc0002658.1_g000032 Rmu_sc0019422.1_g000002
rosa_roxburghii Rroxscaffold_1G00011510
rosa_rugosa Rorug05G0397600
rosa_samantha Rh5AG454900 Rh5BG473900 Rh5CG496300 Rh5DG485700
rosa_wichuraiana Rw5G042430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 16
AccBSI CCGCTC 1 cut(s) 472
AciI CCGC 2 cut(s) 463, 470
AclWI GGATC 3 cut(s) 78, 354, 438
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 356
AfaI GTAC 2 cut(s) 98, 191
AfiI CCNNNNNNNGG 2 cut(s) 85, 314
AgsI TTSAA 5 cut(s) 65, 126, 164, 353, 383
AjnI CCWGG 1 cut(s) 394
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AluBI AGCT 2 cut(s) 36, 328
AluI AGCT 2 cut(s) 36, 328
AlwI GGATC 3 cut(s) 78, 354, 438
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 432
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 2 cut(s) 30, 363
AspS9I GGNCC 1 cut(s) 451
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 229, 297
AvaII GGWCC 1 cut(s) 451
BanI GGYRCC 1 cut(s) 16
BbsI GAAGAC 1 cut(s) 227
BbvI GCAGC 1 cut(s) 419
BciT130I CCWGG 1 cut(s) 396
BciVI GTATCC 1 cut(s) 477
BcnI CCSGG 1 cut(s) 215
BfaI CTAG 2 cut(s) 80, 245
BfoI RGCGCY 2 cut(s) 31, 364
BfuI GTATCC 1 cut(s) 477
BisI GCNGC 1 cut(s) 433
BlsI GCNGC 1 cut(s) 434
Bme1390I CCNGG 2 cut(s) 215, 396
Bme18I GGWCC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 451
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 2 cut(s) 215, 396
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 2 cut(s) 180, 311
BmuI ACTGGG 1 cut(s) 412
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 215
Bsa29I ATCGAT 2 cut(s) 334, 442
BsaJI CCNNGG 4 cut(s) 342, 394, 395, 447
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 314
Bse1I ACTGG 2 cut(s) 418, 430
BseBI CCWGG 1 cut(s) 396
BseCI ATCGAT 2 cut(s) 334, 442
BseDI CCNNGG 4 cut(s) 342, 394, 395, 447
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 2 cut(s) 85, 314
BseNI ACTGG 2 cut(s) 418, 430
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 1 cut(s) 419
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 16
BshVI ATCGAT 2 cut(s) 334, 442
BsiEI CGRYCG 1 cut(s) 334
BsiSI CCGG 2 cut(s) 20, 215
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 85, 314
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 1 cut(s) 5
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 5 cut(s) 70, 331, 346, 439, 443
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 2 cut(s) 463, 470
BspANI GGCC 1 cut(s) 5
BspDI ATCGAT 2 cut(s) 334, 442
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 18
BspPI GGATC 3 cut(s) 78, 354, 438
BspT107I GGYRCC 1 cut(s) 16
BsrBI CCGCTC 1 cut(s) 472
BsrGI TGTACA 1 cut(s) 96
BsrI ACTGG 2 cut(s) 418, 430
BssECI CCNNGG 4 cut(s) 342, 394, 395, 447
BssMI GATC 5 cut(s) 70, 331, 346, 439, 443
BssT1I CCWWGG 2 cut(s) 342, 447
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 381
BstAUI TGTACA 1 cut(s) 96
BstDSI CCRYGG 1 cut(s) 342
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 1 cut(s) 256
BstH2I RGCGCY 2 cut(s) 31, 364
BstHHI GCGC 2 cut(s) 30, 363
BstKTI GATC 5 cut(s) 73, 334, 349, 442, 446
BstMBI GATC 5 cut(s) 70, 331, 346, 439, 443
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 3 cut(s) 177, 367, 469
BstNI CCWGG 1 cut(s) 396
BstPAI GACNNNNGTC 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 213, 394
BstV1I GCAGC 1 cut(s) 419
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 346
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 2 cut(s) 334, 442
BsuI GTATCC 1 cut(s) 477
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 2 cut(s) 334, 442
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 2 cut(s) 269, 423
CciI TCATGA 1 cut(s) 199
CfoI GCGC 2 cut(s) 30, 363
Cfr13I GGNCC 1 cut(s) 451
ClaI ATCGAT 2 cut(s) 334, 442
Csp6I GTAC 2 cut(s) 97, 190
CviAII CATG 3 cut(s) 200, 297, 343
CviJI RGCY 5 cut(s) 5, 36, 328, 416, 432
CviKI_1 RGCY 5 cut(s) 5, 36, 328, 416, 432
CviQI GTAC 2 cut(s) 97, 190
DpnI GATC 5 cut(s) 72, 333, 348, 441, 445
DpnII GATC 5 cut(s) 70, 331, 346, 439, 443
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 342, 447
Eco47I GGWCC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 356
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 394
EcoT14I CCWWGG 2 cut(s) 342, 447
ErhI CCWWGG 2 cut(s) 342, 447
FaeI CATG 3 cut(s) 203, 300, 346
FaqI GGGAC 1 cut(s) 296
FatI CATG 3 cut(s) 199, 296, 342
Fnu4HI GCNGC 1 cut(s) 433
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 1 cut(s) 433
FspBI CTAG 2 cut(s) 80, 245
GlaI GCGC 2 cut(s) 29, 362
GluI GCNGC 1 cut(s) 433
GsuI CTGGAG 1 cut(s) 447
HaeII RGCGCY 2 cut(s) 31, 364
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 20, 215
HhaI GCGC 2 cut(s) 30, 363
Hin1II CATG 3 cut(s) 203, 300, 346
Hin6I GCGC 2 cut(s) 28, 361
HinP1I GCGC 2 cut(s) 28, 361
HpaII CCGG 2 cut(s) 20, 215
HphI GGTGA 2 cut(s) 229, 297
Hpy166II GTNNAC 3 cut(s) 43, 107, 423
Hpy188I TCNGA 4 cut(s) 54, 294, 375, 476
Hpy188III TCNNGA 4 cut(s) 200, 238, 350, 383
Hpy8I GTNNAC 3 cut(s) 43, 107, 423
HpyAV CCTTC 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 3 cut(s) 141, 262, 274
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 367, 469
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 3 cut(s) 203, 300, 346
HspAI GCGC 2 cut(s) 28, 361
Kzo9I GATC 5 cut(s) 70, 331, 346, 439, 443
LpnPI CCDG 8 cut(s) 33, 223, 228, 381, 408, 411, 431, 450
Lsp1109I GCAGC 1 cut(s) 419
LweI GCATC 2 cut(s) 180, 311
MaeI CTAG 2 cut(s) 80, 245
MaeII ACGT 1 cut(s) 159
MalI GATC 5 cut(s) 72, 333, 348, 441, 445
MbiI CCGCTC 1 cut(s) 472
MboI GATC 5 cut(s) 70, 331, 346, 439, 443
MboII GAAGA 2 cut(s) 232, 398
MflI RGATCY 1 cut(s) 346
MluCI AATT 3 cut(s) 11, 131, 144
MnlI CCTC 8 cut(s) 76, 79, 195, 203, 241, 273, 313, 422
MspI CCGG 2 cut(s) 20, 215
MspR9I CCNGG 2 cut(s) 215, 396
MvaI CCWGG 1 cut(s) 396
MwoI GCNNNNNNNGC 3 cut(s) 177, 367, 469
NciI CCSGG 1 cut(s) 215
NcoI CCATGG 1 cut(s) 342
NdeII GATC 5 cut(s) 70, 331, 346, 439, 443
NlaIII CATG 3 cut(s) 203, 300, 346
NlaIV GGNNCC 1 cut(s) 18
NmeAIII GCCGAG 1 cut(s) 31
PagI TCATGA 1 cut(s) 199
PasI CCCWGGG 1 cut(s) 395
PkrI GCNGC 1 cut(s) 434
Ple19I CGATCG 1 cut(s) 334
PpuMI RGGWCCY 1 cut(s) 451
PshAI GACNNNNGTC 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 451
Psp6I CCWGG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 451
PspPPI RGGWCCY 1 cut(s) 451
PsuI RGATCY 1 cut(s) 346
PvuI CGATCG 1 cut(s) 334
RsaI GTAC 2 cut(s) 98, 191
RsaNI GTAC 2 cut(s) 97, 190
SatI GCNGC 1 cut(s) 433
Sau3AI GATC 5 cut(s) 70, 331, 346, 439, 443
Sau96I GGNCC 1 cut(s) 451
ScrFI CCNGG 2 cut(s) 215, 396
SetI ASST 8 cut(s) 38, 90, 162, 195, 207, 292, 330, 456
SfaNI GCATC 2 cut(s) 180, 311
SinI GGWCC 1 cut(s) 451
Sse9I AATT 3 cut(s) 11, 131, 144
SsiI CCGC 2 cut(s) 463, 470
SspMI CTAG 2 cut(s) 80, 245
StyD4I CCNGG 2 cut(s) 213, 394
StyI CCWWGG 2 cut(s) 342, 447
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 152, 330, 334, 442
TasI AATT 3 cut(s) 11, 131, 144
TatI WGTACW 1 cut(s) 96
TscAI CASTG 2 cut(s) 276, 430
TseI GCWGC 1 cut(s) 432
TspGWI ACGGA 2 cut(s) 177, 239
TspRI CASTG 2 cut(s) 276, 430
VpaK11BI GGWCC 1 cut(s) 451
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 1 cut(s) 144
XspI CTAG 2 cut(s) 80, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.