pycom03g22210

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0022536
Physical Location & Seq
Forward (+)
22388536 .. 22390759
2224 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGGCCGCAATGAGTGGCCTCGCTACGAGCTCCACTCCTTGTCGGTTACTCGCATTTCCAATACGACGCCGTTTCCGGGACCACTCGTCTTCGCGCTCCGTGGTCATCAGTAAACCTCCTTCCAGAGCTCCCAGACTCATTTGCATGGCGGAGCCTTATTTGATTACAAAGCTTGATTCTGCAGAGAAGACGTGGAAGGATTTATCGGTCAAGCTGGCTGATCCAGATGTCGTGTCAAATCCCAGTGAATACCAGAAGCTTGCGCAGTCTGTGGCAGAGCTTGATGAGGTCGTGACAATCTATAGGGATTTCAAAGACTGCGAGAAGCAGTTAGAAGAGACTAAAGCTTTAGCAAAAGAAGCTGGCAATGATGAGGATATGGCAGAGATGATAGCTTTTGAACTTGAATCTTTATCCAATCAAATCAAAGAACTTGAAGCGAAGCTCAAGATGCTACTCCTTCCTAGTGACCCTCTTGATGCAAGAAATATAATGCTTGAAGTAAGGGCTGGTACTGGTGGTGATGAAGCTGGAATTTGGGCAGGTCGATCTTGTCCGTATGTATGA

Protein Analysis

189

Amino Acids

20.85

Weight (kDa)

5.11

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 62 - 182 3.1e-30 PCRF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022536)

Species Orthologous Gene IDs
pyrus_communis pycom03g22210
rosa_chinensis RchiOBHm_Chr6g0260181
rosa_samantha Rh4AG093700
rosa_wichuraiana Rw2G010120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 264
Acc36I ACCTGC 1 cut(s) 533
AccII CGCG 1 cut(s) 94
AciI CCGC 2 cut(s) 6, 149
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 534
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 1 cut(s) 514
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 5 cut(s) 313, 401, 407, 437, 500
AhdI GACNNNNNGTC 1 cut(s) 85
AjiI CACGTC 1 cut(s) 192
Alw21I GWGCWC 2 cut(s) 32, 130
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 1 cut(s) 215
AoxI GGCC 2 cut(s) 3, 16
ApoI RAATTY 1 cut(s) 534
AspLEI GCGC 2 cut(s) 96, 265
AspS9I GGNCC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 533
AvaII GGWCC 1 cut(s) 79
BanII GRGCYC 2 cut(s) 32, 130
BarI GAAGNNNNNNTAC 2 cut(s) 103, 135
BbsI GAAGAC 2 cut(s) 81, 194
Bbv12I GWGCWC 2 cut(s) 32, 130
BceAI ACGGC 1 cut(s) 54
BcnI CCSGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 2 cut(s) 180, 301
BfuAI ACCTGC 1 cut(s) 533
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 79
BmeRI GACNNNNNGTC 1 cut(s) 85
BmgBI CACGTC 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 80, 153
BmrFI CCNGG 1 cut(s) 77
BmrI ACTGGG 1 cut(s) 237
BmsI GCATC 2 cut(s) 441, 469
BmuI ACTGGG 1 cut(s) 237
BpiI GAAGAC 2 cut(s) 81, 194
BplI GAGNNNNNCTC 2 cut(s) 19, 51
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 243, 520
Bse3DI GCAATG 2 cut(s) 15, 373
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMI GCAATG 2 cut(s) 15, 373
BseNI ACTGG 2 cut(s) 243, 520
Bsh1236I CGCG 1 cut(s) 94
BshFI GGCC 2 cut(s) 5, 18
BsiHKAI GWGCWC 2 cut(s) 32, 130
BsiSI CCGG 1 cut(s) 76
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 2 cut(s) 5, 18
Bsp1286I GDGCHC 2 cut(s) 32, 130
Bsp143I GATC 2 cut(s) 220, 548
BspACI CCGC 2 cut(s) 6, 149
BspANI GGCC 2 cut(s) 5, 18
BspFNI CGCG 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 80, 153
BspMAI CTGCAG 1 cut(s) 184
BspMI ACCTGC 1 cut(s) 533
BspPI GGATC 1 cut(s) 215
BsrDI GCAATG 2 cut(s) 15, 373
BsrI ACTGG 2 cut(s) 243, 520
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 2 cut(s) 220, 548
BssNI GRCGYC 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 67
BstC8I GCNNGC 3 cut(s) 216, 261, 364
BstDSI CCRYGG 1 cut(s) 99
BstFNI CGCG 1 cut(s) 94
BstHHI GCGC 2 cut(s) 96, 265
BstKTI GATC 2 cut(s) 223, 551
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 2 cut(s) 220, 548
BstMWI GCNNNNNNNGC 2 cut(s) 359, 451
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 2 cut(s) 180, 301
BstUI CGCG 1 cut(s) 94
BstV2I GAAGAC 2 cut(s) 81, 194
BsuRI GGCC 2 cut(s) 5, 18
BtgI CCRYGG 1 cut(s) 99
BtrI CACGTC 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 250
BveI ACCTGC 1 cut(s) 533
Cac8I GCNNGC 3 cut(s) 216, 261, 364
CfoI GCGC 2 cut(s) 96, 265
Cfr13I GGNCC 1 cut(s) 79
CseI GACGC 1 cut(s) 75
Csp6I GTAC 1 cut(s) 513
CviAII CATG 1 cut(s) 145
CviQI GTAC 1 cut(s) 513
DpnI GATC 2 cut(s) 222, 550
DpnII GATC 2 cut(s) 220, 548
DriI GACNNNNNGTC 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 330
Eam1105I GACNNNNNGTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 164
Ecl136II GAGCTC 2 cut(s) 30, 128
Eco24I GRGCYC 2 cut(s) 32, 130
Eco47I GGWCC 1 cut(s) 79
Eco53kI GAGCTC 2 cut(s) 30, 128
EcoICRI GAGCTC 2 cut(s) 30, 128
EcoT38I GRGCYC 2 cut(s) 32, 130
FaeI CATG 1 cut(s) 148
FaiI YATR 6 cut(s) 146, 303, 380, 491, 561, 565
FaqI GGGAC 1 cut(s) 92
FatI CATG 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 6
FriOI GRGCYC 2 cut(s) 32, 130
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 465
FspI TGCGCA 1 cut(s) 264
GlaI GCGC 2 cut(s) 95, 264
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 2 cut(s) 5, 18
HapII CCGG 1 cut(s) 76
HgaI GACGC 1 cut(s) 75
HhaI GCGC 2 cut(s) 96, 265
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 1 cut(s) 148
Hin6I GCGC 2 cut(s) 94, 263
HinP1I GCGC 2 cut(s) 94, 263
HindIII AAGCTT 3 cut(s) 170, 257, 345
HinfI GANTC 3 cut(s) 135, 176, 407
HpaII CCGG 1 cut(s) 76
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 1 cut(s) 113
Hpy188III TCNNGA 5 cut(s) 123, 224, 292, 448, 476
Hpy8I GTNNAC 1 cut(s) 113
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 3 cut(s) 129, 190, 470
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 3 cut(s) 144, 182, 482
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 451
HpySE526I ACGT 1 cut(s) 191
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 1 cut(s) 148
HspAI GCGC 2 cut(s) 94, 263
Kzo9I GATC 2 cut(s) 220, 548
LmnI GCTCC 4 cut(s) 35, 101, 133, 151
LweI GCATC 2 cut(s) 441, 469
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 3 cut(s) 45, 292, 467
MalI GATC 2 cut(s) 222, 550
MboI GATC 2 cut(s) 220, 548
MboII GAAGA 3 cut(s) 81, 199, 347
MhlI GDGCHC 2 cut(s) 32, 130
MluCI AATT 1 cut(s) 534
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 5 cut(s) 29, 126, 280, 367, 483
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 2 cut(s) 359, 451
NciI CCSGG 1 cut(s) 77
NdeII GATC 2 cut(s) 220, 548
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 2 cut(s) 80, 153
NmuCI GTSAC 2 cut(s) 292, 467
NsbI TGCGCA 1 cut(s) 264
PfeI GAWTC 2 cut(s) 176, 407
PfoI TCCNGGA 1 cut(s) 75
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
Psp124BI GAGCTC 2 cut(s) 32, 130
PspN4I GGNNCC 2 cut(s) 80, 153
PspPI GGNCC 1 cut(s) 79
PstI CTGCAG 1 cut(s) 184
RsaI GTAC 1 cut(s) 514
RsaNI GTAC 1 cut(s) 513
RseI CAYNNNNRTG 1 cut(s) 143
SacI GAGCTC 2 cut(s) 32, 130
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 2 cut(s) 220, 548
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 2 cut(s) 32, 130
SfaNI GCATC 2 cut(s) 441, 469
SfcI CTRYAG 2 cut(s) 180, 301
SinI GGWCC 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 1 cut(s) 534
SsiI CCGC 2 cut(s) 6, 149
SspMI CTAG 1 cut(s) 465
SstI GAGCTC 2 cut(s) 32, 130
StyD4I CCNGG 1 cut(s) 75
TaiI ACGT 1 cut(s) 194
TaqI TCGA 1 cut(s) 547
TaqII GACCGA 1 cut(s) 196
TasI AATT 1 cut(s) 534
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 176, 407
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 2 cut(s) 292, 467
Tsp45I GTSAC 2 cut(s) 292, 467
TspDTI ATGAA 1 cut(s) 540
TspGWI ACGGA 2 cut(s) 88, 546
TspRI CASTG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 1 cut(s) 534
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.