RchiOBHm_Chr6g0260181

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0022536
Physical Location & Seq
Reverse (-)
15271196 .. 15272207
1012 bp
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UTR
Exon/CDS
Intron
PRQ23331

Sequence Viewer

Length: 462 bp
ATGAGTGGCCTCACCACCACCTCCGCACCGCCGCGATTACTCGCAATCCCAACACGACACCGTTTCACCGACAATTCATGGACTTCCATCGCCATCCCCAAACCTCATCTCAGAGCTCCACGAATCATTTGCATGGCGGCGCCGTATTTGATTACGAAGCTGGAATCTGCAGAGAAGACACAGAAGGAATTATCGGTCAAGCTGGCTGATCCAGATGTCGTGTCGAATCCCAGTGAGTACCAGAGGTTGGCACAATCCGTGGCGGTCTCGATGAGGTACTGCAGAGTTGTGTCAATCTATAAGGAATATAAGGACTGTGAGAAGCAGTTAGAAGAGAGTAAAGCTTTAGTAGATGAAGATGGCAATGATGAAGAAATGGCAGCAATGATATCGTATGAAATTGAGTCTTTGTCCAATCAAATGAAAGAACTTGAGGAGAAGCTTAAGGCTATAAGCATTTAA

Protein Analysis

153

Amino Acids

17.26

Weight (kDa)

5.51

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 59 - 150 2.1e-09 PCRF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022536)

Species Orthologous Gene IDs
pyrus_communis pycom03g22210
rosa_chinensis RchiOBHm_Chr6g0260181
rosa_samantha Rh4AG093700
rosa_wichuraiana Rw2G010120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 247
AccII CGCG 1 cut(s) 34
AciI CCGC 5 cut(s) 24, 29, 32, 137, 263
AclWI GGATC 1 cut(s) 203
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 2 cut(s) 239, 278
AfiI CCNNNNNNNGG 1 cut(s) 247
AflII CTTAAG 1 cut(s) 443
AluBI AGCT 5 cut(s) 116, 160, 202, 344, 442
AluI AGCT 5 cut(s) 116, 160, 202, 344, 442
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 1 cut(s) 271
AlwI GGATC 1 cut(s) 203
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 1 cut(s) 380
AspLEI GCGC 1 cut(s) 142
AsuHPI GGTGA 2 cut(s) 4, 58
BanI GGYRCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 1 cut(s) 392
BccI CCATC 3 cut(s) 95, 101, 353
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 4 cut(s) 111, 145, 372, 406
BcoDI GTCTC 1 cut(s) 271
BfmI CTRYAG 2 cut(s) 168, 280
BfoI RGCGCY 1 cut(s) 143
BfrI CTTAAG 1 cut(s) 443
BisI GCNGC 3 cut(s) 32, 138, 381
BlsI GCNGC 3 cut(s) 33, 139, 382
BmiI GGNNCC 1 cut(s) 141
BmrI ACTGGG 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 452
BsaHI GRCGYC 1 cut(s) 140
BsaI GGTCTC 1 cut(s) 271
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 2 cut(s) 370, 390
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMI GCAATG 2 cut(s) 370, 390
BseMII CTCAG 1 cut(s) 124
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 1 cut(s) 392
Bsh1236I CGCG 1 cut(s) 34
BshFI GGCC 1 cut(s) 9
BshNI GGYRCC 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 1 cut(s) 271
BsnI GGCC 1 cut(s) 9
Bso31I GGTCTC 1 cut(s) 271
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 5 cut(s) 24, 29, 32, 137, 263
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 1 cut(s) 123
BspFNI CGCG 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 141
BspMAI CTGCAG 2 cut(s) 172, 284
BspPI GGATC 1 cut(s) 203
BspT107I GGYRCC 1 cut(s) 139
BspTI CTTAAG 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 271
BsrDI GCAATG 2 cut(s) 370, 390
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 1 cut(s) 208
BssNI GRCGYC 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 62, 317
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 1 cut(s) 140
BstAFI CTTAAG 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 110
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 1 cut(s) 211
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 1 cut(s) 208
BstSFI CTRYAG 2 cut(s) 168, 280
BstUI CGCG 1 cut(s) 34
BstV1I GCAGC 1 cut(s) 392
BstV2I GAAGAC 1 cut(s) 182
BsuRI GGCC 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 258
BtgZI GCGATG 1 cut(s) 73
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 238, 277
CviAII CATG 2 cut(s) 78, 133
CviJI RGCY 8 cut(s) 9, 116, 160, 202, 206, 344, 442, 449
CviKI_1 RGCY 8 cut(s) 9, 116, 160, 202, 206, 344, 442, 449
CviQI GTAC 2 cut(s) 238, 277
DdeI CTNAG 1 cut(s) 110
DinI GGCGCC 1 cut(s) 141
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 271
Eco32I GATATC 1 cut(s) 390
Eco53kI GAGCTC 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 116
EcoRV GATATC 1 cut(s) 390
EcoT38I GRGCYC 1 cut(s) 118
EgeI GGCGCC 1 cut(s) 141
EheI GGCGCC 1 cut(s) 141
FaeI CATG 2 cut(s) 81, 136
FaiI YATR 6 cut(s) 79, 134, 300, 309, 396, 452
FatI CATG 2 cut(s) 77, 132
Fnu4HI GCNGC 3 cut(s) 32, 138, 381
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 1 cut(s) 118
Fsp4HI GCNGC 3 cut(s) 32, 138, 381
GlaI GCGC 1 cut(s) 141
GluI GCNGC 3 cut(s) 32, 138, 381
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 1 cut(s) 9
HhaI GCGC 1 cut(s) 142
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 2 cut(s) 81, 136
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HindIII AAGCTT 2 cut(s) 342, 440
HinfI GANTC 4 cut(s) 123, 164, 226, 404
HphI GGTGA 2 cut(s) 4, 58
Hpy188I TCNGA 1 cut(s) 113
Hpy188III TCNNGA 2 cut(s) 212, 268
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 2 cut(s) 62, 317
HpyCH4V TGCA 3 cut(s) 132, 170, 282
HpyF3I CTNAG 1 cut(s) 110
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 2 cut(s) 81, 136
HspAI GCGC 1 cut(s) 140
KasI GGCGCC 1 cut(s) 139
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 5 cut(s) 146, 188, 225, 244, 254
Lsp1109I GCAGC 1 cut(s) 392
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 4 cut(s) 187, 344, 368, 383
MhlI GDGCHC 1 cut(s) 118
MluCI AATT 3 cut(s) 73, 188, 399
Mly113I GGCGCC 1 cut(s) 140
MlyI GAGTC 1 cut(s) 413
MnlI CCTC 6 cut(s) 20, 31, 114, 237, 267, 427
MseI TTAA 2 cut(s) 444, 460
MslI CAYNNNNRTG 1 cut(s) 131
MspCI CTTAAG 1 cut(s) 443
MvnI CGCG 1 cut(s) 34
NarI GGCGCC 1 cut(s) 140
NdeII GATC 1 cut(s) 208
NlaIII CATG 2 cut(s) 81, 136
NlaIV GGNNCC 1 cut(s) 141
PfeI GAWTC 3 cut(s) 123, 164, 226
PflMI CCANNNNNTGG 1 cut(s) 247
PkrI GCNGC 3 cut(s) 33, 139, 382
PleI GAGTC 1 cut(s) 412
PluTI GGCGCC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 412
Psp124BI GAGCTC 1 cut(s) 118
PspN4I GGNNCC 1 cut(s) 141
PstI CTGCAG 2 cut(s) 172, 284
RsaI GTAC 2 cut(s) 239, 278
RsaNI GTAC 2 cut(s) 238, 277
RseI CAYNNNNRTG 1 cut(s) 131
SacI GAGCTC 1 cut(s) 118
SaqAI TTAA 2 cut(s) 444, 460
SatI GCNGC 3 cut(s) 32, 138, 381
Sau3AI GATC 1 cut(s) 208
SchI GAGTC 1 cut(s) 413
SduI GDGCHC 1 cut(s) 118
SetI ASST 9 cut(s) 23, 106, 118, 162, 204, 248, 278, 346, 444
SfcI CTRYAG 2 cut(s) 168, 280
SfoI GGCGCC 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 2 cut(s) 431, 443
SmoI CTYRAG 2 cut(s) 431, 443
Sse9I AATT 3 cut(s) 73, 188, 399
SsiI CCGC 5 cut(s) 24, 29, 32, 137, 263
SspDI GGCGCC 1 cut(s) 139
SstI GAGCTC 1 cut(s) 118
TaaI ACNGT 2 cut(s) 62, 317
TaqI TCGA 2 cut(s) 224, 269
TaqII GACCGA 1 cut(s) 184
TasI AATT 3 cut(s) 73, 188, 399
TauI GCSGC 2 cut(s) 34, 140
TfiI GAWTC 3 cut(s) 123, 164, 226
Tru1I TTAA 2 cut(s) 444, 460
Tru9I TTAA 2 cut(s) 444, 460
TscAI CASTG 1 cut(s) 238
TseI GCWGC 1 cut(s) 380
TspDTI ATGAA 5 cut(s) 66, 369, 384, 411, 437
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 1 cut(s) 238
Van91I CCANNNNNTGG 1 cut(s) 247
Vha464I CTTAAG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.