pycom04g00880

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0020976
Physical Location & Seq
Reverse (-)
762814 .. 763871
1058 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 885 bp
ATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGACCAACCCTTCTTGAGGCTCTTGACCTGATCAACGAGCCCAAGAGGCCCTCAGACAAGCCCCTCCGTCTTCCTCTTCAGGATGTGTACAAGATTGGTGGTATTGGAACTGTGCCAGTGGGACGTGTAGAGACTGGTATAGTCAAGCCTGGTATGGTTGTCACTTTTGGTCCAACTGGTCTGACCACTGAAGTTAAGTCAGTTGAGATGCATCATGAGGCTCTTCAGGAGGCACTCCCCGGTGACAATGTCGGGTTCAATGTTAAGAATGTTGCTGTGAAGGATCGATCCAAAGGCATAATTCCGATCATCAAGCGTGGTTTCGTTGCATCCAACTCCAAGGACGACCCTGCCAAGGAGGCTGCCAACTTCACATCCCAGGTTATCATCATGAACCACCCTGGTCAGATCGGTAACGGATATGCCCCAGTTCTGGATTGCCACACTTCCCACATTGCTGTGAAGTTCGGTGAGATCCTTACCAAGATTGACAGGAGGTCTGGAAAGGAGATTGAGAAGGAGCCAAAGTTCTTGAAGAACGGAGATGCAGGTATGGTGAAGATGCTTCCCACCAAGCCCATGGTTGTTGAGACCTTCTCTGAGTACCCACCATTGGGTCGCTTTGCTGTTCGTGACATGCGCCAGACTGTTGCTGTCGGTGTAATCAAGAGCGTTGAGAAGAACGATCCCAGCGGTGCCAAGTGTCCACTCCCAGAATTGGGTGCTGGACAGGCGGTGGTGACGCATCTGATTTATGCTTTCAGTCGCTTCCGCCTTCGGGAGGAGGATGTTTTTGAGTTGTTGAGACTATTTACTTTTCAAGTTTTGTTGCGTCTGTTGAAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

295

Amino Acids

32.38

Weight (kDa)

9.18

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU_D2 PF03144 48 - 111 1.4e-15 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 130 - 237 4.5e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 135 - 235 3.4e-37 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35550
malus_domestica MD12G1214600.v1.1 MD16G1024200.v1.1 MD17G1192800.v1.1
pyrus_communis pycom04g00880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 731
AciI CCGC 3 cut(s) 729, 770, 808
AclWI GGATC 4 cut(s) 318, 327, 505, 716
AcuI CTGAAG 3 cut(s) 98, 245, 246
AfaI GTAC 3 cut(s) 32, 125, 641
AfiI CCNNNNNNNGG 9 cut(s) 52, 391, 469, 649, 650, 754, 755, 814, 817
AflIII ACRYGT 1 cut(s) 160
AgsI TTSAA 4 cut(s) 295, 571, 857, 877
AhdI GACNNNNNGTC 1 cut(s) 532
AjiI CACGTC 1 cut(s) 161
AjnI CCWGG 3 cut(s) 184, 414, 436
AloI GAACNNNNNNTCC 2 cut(s) 548, 580
Alw26I GTCTC 3 cut(s) 161, 620, 835
AlwI GGATC 4 cut(s) 318, 327, 505, 716
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 398
AspLEI GCGC 1 cut(s) 678
AspS9I GGNCC 4 cut(s) 12, 38, 84, 206
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 4 cut(s) 290, 518, 604, 787
AvaII GGWCC 3 cut(s) 12, 38, 206
BanI GGYRCC 1 cut(s) 731
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 98
BbvI GCAGC 1 cut(s) 385
BciT130I CCWGG 3 cut(s) 186, 416, 438
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 1 cut(s) 276
BcoDI GTCTC 3 cut(s) 161, 620, 835
BfuAI ACCTGC 1 cut(s) 575
BglI GCCNNNNNGGC 2 cut(s) 82, 395
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
Bme1390I CCNGG 4 cut(s) 186, 276, 416, 438
Bme18I GGWCC 3 cut(s) 12, 38, 206
BmeRI GACNNNNNGTC 1 cut(s) 532
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 4 cut(s) 12, 38, 84, 206
BmiI GGNNCC 3 cut(s) 39, 558, 733
BmrFI CCNGG 4 cut(s) 186, 276, 416, 438
BmrI ACTGGG 1 cut(s) 458
BmsI GCATC 6 cut(s) 234, 256, 374, 571, 588, 790
BmuI ACTGGG 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 98
BplI GAGNNNNNCTC 2 cut(s) 617, 649
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 322
BsaI GGTCTC 1 cut(s) 620
BsaJI CCNNGG 6 cut(s) 274, 375, 390, 414, 436, 615
BsaXI ACNNNNNCTCC 2 cut(s) 548, 578
Bsc4I CCNNNNNNNGG 9 cut(s) 52, 391, 469, 649, 650, 754, 755, 814, 817
Bse1I ACTGG 5 cut(s) 32, 152, 175, 217, 464
Bse3DI GCAATG 1 cut(s) 489
BseBI CCWGG 3 cut(s) 186, 416, 438
BseCI ATCGAT 1 cut(s) 322
BseDI CCNNGG 6 cut(s) 274, 375, 390, 414, 436, 615
BseGI GGATG 4 cut(s) 124, 365, 410, 829
BseLI CCNNNNNNNGG 9 cut(s) 52, 391, 469, 649, 650, 754, 755, 814, 817
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 2 cut(s) 102, 627
BseNI ACTGG 5 cut(s) 32, 152, 175, 217, 464
BseRI GAGGAG 1 cut(s) 833
BseXI GCAGC 1 cut(s) 385
BseYI CCCAGC 1 cut(s) 725
BshFI GGCC 1 cut(s) 85
BshNI GGYRCC 1 cut(s) 731
BshVI ATCGAT 1 cut(s) 322
BsiSI CCGG 1 cut(s) 276
BslFI GGGAC 2 cut(s) 51, 171
BslI CCNNNNNNNGG 9 cut(s) 52, 391, 469, 649, 650, 754, 755, 814, 817
BsmAI GTCTC 3 cut(s) 161, 620, 835
BsmFI GGGAC 2 cut(s) 51, 171
BsnI GGCC 1 cut(s) 85
Bso31I GGTCTC 1 cut(s) 620
Bsp1286I GDGCHC 1 cut(s) 78
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 7 cut(s) 66, 319, 323, 342, 444, 510, 721
Bsp19I CCATGG 1 cut(s) 615
BspACI CCGC 3 cut(s) 729, 770, 808
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 2 cut(s) 101, 628
BspDI ATCGAT 1 cut(s) 322
BspHI TCATGA 2 cut(s) 250, 426
BspLI GGNNCC 3 cut(s) 39, 558, 733
BspMI ACCTGC 1 cut(s) 575
BspPI GGATC 4 cut(s) 318, 327, 505, 716
BspQI GCTCTTC 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 731
BspTNI GGTCTC 1 cut(s) 620
BsrDI GCAATG 1 cut(s) 489
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 5 cut(s) 32, 152, 175, 217, 464
BssECI CCNNGG 6 cut(s) 274, 375, 390, 414, 436, 615
BssMI GATC 7 cut(s) 66, 319, 323, 342, 444, 510, 721
BssT1I CCWWGG 3 cut(s) 375, 390, 615
Bst2UI CCWGG 3 cut(s) 186, 416, 438
Bst4CI ACNGT 2 cut(s) 148, 685
Bst6I CTCTTC 2 cut(s) 117, 264
BstAUI TGTACA 1 cut(s) 123
BstDEI CTNAG 2 cut(s) 88, 636
BstDSI CCRYGG 1 cut(s) 615
BstENI CCTNNNNNAGG 2 cut(s) 50, 815
BstF5I GGATG 4 cut(s) 124, 365, 410, 829
BstHHI GCGC 1 cut(s) 678
BstKTI GATC 7 cut(s) 69, 322, 326, 345, 447, 513, 724
BstMAI GTCTC 3 cut(s) 161, 620, 835
BstMBI GATC 7 cut(s) 66, 319, 323, 342, 444, 510, 721
BstMWI GCNNNNNNNGC 3 cut(s) 82, 395, 767
BstNI CCWGG 3 cut(s) 186, 416, 438
BstNSI RCATGY 1 cut(s) 676
BstSCI CCNGG 4 cut(s) 184, 274, 414, 436
BstV1I GCAGC 1 cut(s) 385
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 510
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 1 cut(s) 510
Bsu15I ATCGAT 1 cut(s) 322
BsuRI GGCC 1 cut(s) 85
BsuTUI ATCGAT 1 cut(s) 322
BtgI CCRYGG 1 cut(s) 615
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 4 cut(s) 124, 365, 410, 829
BtsIMutI CAGTG 2 cut(s) 159, 222
BveI ACCTGC 1 cut(s) 575
CciI TCATGA 2 cut(s) 250, 426
CfoI GCGC 1 cut(s) 678
Cfr13I GGNCC 4 cut(s) 12, 38, 84, 206
ClaI ATCGAT 1 cut(s) 322
CseI GACGC 2 cut(s) 787, 857
Csp6I GTAC 3 cut(s) 31, 124, 640
CspCI CAANNNNNGTGG 4 cut(s) 4, 39, 115, 150
CviAII CATG 4 cut(s) 251, 427, 616, 673
CviJI RGCY 9 cut(s) 56, 76, 85, 97, 184, 257, 398, 559, 613
CviKI_1 RGCY 9 cut(s) 56, 76, 85, 97, 184, 257, 398, 559, 613
CviQI GTAC 3 cut(s) 31, 124, 640
DdeI CTNAG 2 cut(s) 88, 636
DpnI GATC 7 cut(s) 68, 321, 325, 344, 446, 512, 723
DpnII GATC 7 cut(s) 66, 319, 323, 342, 444, 510, 721
DriI GACNNNNNGTC 1 cut(s) 532
Eam1104I CTCTTC 2 cut(s) 117, 264
Eam1105I GACNNNNNGTC 1 cut(s) 532
EarI CTCTTC 2 cut(s) 117, 264
EciI GGCGGA 1 cut(s) 797
Eco130I CCWWGG 3 cut(s) 375, 390, 615
Eco24I GRGCYC 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 620
Eco47I GGWCC 3 cut(s) 12, 38, 206
Eco57I CTGAAG 3 cut(s) 98, 245, 246
EcoNI CCTNNNNNAGG 2 cut(s) 50, 815
EcoO109I RGGNCCY 1 cut(s) 84
EcoRII CCWGG 3 cut(s) 184, 414, 436
EcoT14I CCWWGG 3 cut(s) 375, 390, 615
EcoT22I ATGCAT 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 3 cut(s) 375, 390, 615
FaeI CATG 4 cut(s) 254, 430, 619, 676
FaqI GGGAC 2 cut(s) 51, 171
FatI CATG 4 cut(s) 250, 426, 615, 672
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 4 cut(s) 131, 352, 397, 836
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 399
GlaI GCGC 1 cut(s) 677
GluI GCNGC 1 cut(s) 399
GsaI CCCAGC 1 cut(s) 729
HaeIII GGCC 1 cut(s) 85
HapII CCGG 1 cut(s) 276
HgaI GACGC 2 cut(s) 787, 857
HhaI GCGC 1 cut(s) 678
Hin1II CATG 4 cut(s) 254, 430, 619, 676
Hin6I GCGC 1 cut(s) 676
HinP1I GCGC 1 cut(s) 676
HpaII CCGG 1 cut(s) 276
HphI GGTGA 4 cut(s) 290, 518, 604, 787
Hpy166II GTNNAC 3 cut(s) 15, 124, 743
Hpy188I TCNGA 6 cut(s) 91, 219, 342, 444, 637, 786
Hpy8I GTNNAC 3 cut(s) 15, 124, 743
HpyAV CCTTC 6 cut(s) 56, 310, 547, 640, 821, 871
HpyCH4III ACNGT 2 cut(s) 148, 685
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 247, 365, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 395, 767
HpyF3I CTNAG 2 cut(s) 88, 636
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 4 cut(s) 254, 430, 619, 676
HspAI GCGC 1 cut(s) 676
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 7 cut(s) 66, 319, 323, 342, 444, 510, 721
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 1 cut(s) 556
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 6 cut(s) 234, 256, 374, 571, 588, 790
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 5 cut(s) 196, 278, 449, 668, 775
MalI GATC 7 cut(s) 68, 321, 325, 344, 446, 512, 723
MboI GATC 7 cut(s) 66, 319, 323, 342, 444, 510, 721
MboII GAAGA 6 cut(s) 98, 104, 251, 583, 607, 727
MflI RGATCY 1 cut(s) 510
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 2 cut(s) 336, 752
MmeI TCCRAC 2 cut(s) 233, 393
Mph1103I ATGCAT 1 cut(s) 249
MseI TTAA 2 cut(s) 231, 300
MslI CAYNNNNRTG 1 cut(s) 494
MspA1I CMGCKG 1 cut(s) 729
MspI CCGG 1 cut(s) 276
MspR9I CCNGG 4 cut(s) 186, 276, 416, 438
MvaI CCWGG 3 cut(s) 186, 416, 438
MwoI GCNNNNNNNGC 3 cut(s) 82, 395, 767
NciI CCSGG 1 cut(s) 276
NcoI CCATGG 1 cut(s) 615
NdeII GATC 7 cut(s) 66, 319, 323, 342, 444, 510, 721
NlaIII CATG 4 cut(s) 254, 430, 619, 676
NlaIV GGNNCC 3 cut(s) 39, 558, 733
NmuCI GTSAC 4 cut(s) 196, 278, 668, 775
NsiI ATGCAT 1 cut(s) 249
NspI RCATGY 1 cut(s) 676
PagI TCATGA 2 cut(s) 250, 426
PciSI GCTCTTC 1 cut(s) 264
PflFI GACNNNGTC 1 cut(s) 284
PkrI GCNGC 1 cut(s) 400
Psp6I CCWGG 3 cut(s) 184, 414, 436
PspFI CCCAGC 1 cut(s) 725
PspGI CCWGG 3 cut(s) 184, 414, 436
PspN4I GGNNCC 3 cut(s) 39, 558, 733
PspPI GGNCC 4 cut(s) 12, 38, 84, 206
PsuI RGATCY 1 cut(s) 510
PsyI GACNNNGTC 1 cut(s) 284
RsaI GTAC 3 cut(s) 32, 125, 641
RsaNI GTAC 3 cut(s) 31, 124, 640
RseI CAYNNNNRTG 1 cut(s) 494
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 231, 300
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 7 cut(s) 66, 319, 323, 342, 444, 510, 721
Sau96I GGNCC 4 cut(s) 12, 38, 84, 206
ScrFI CCNGG 4 cut(s) 186, 276, 416, 438
SduI GDGCHC 1 cut(s) 78
SetI ASST 8 cut(s) 14, 24, 66, 163, 420, 536, 589, 632
SfaNI GCATC 6 cut(s) 234, 256, 374, 571, 588, 790
SinI GGWCC 3 cut(s) 12, 38, 206
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 2 cut(s) 336, 752
SsiI CCGC 3 cut(s) 729, 770, 808
StyD4I CCNGG 4 cut(s) 184, 274, 414, 436
StyI CCWWGG 3 cut(s) 375, 390, 615
TaaI ACNGT 2 cut(s) 148, 685
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 322
TasI AATT 2 cut(s) 336, 752
TatI WGTACW 1 cut(s) 123
Tru1I TTAA 2 cut(s) 231, 300
Tru9I TTAA 2 cut(s) 231, 300
TscAI CASTG 2 cut(s) 159, 229
TseFI GTSAC 4 cut(s) 196, 278, 668, 775
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 4 cut(s) 196, 278, 668, 775
TspDTI ATGAA 1 cut(s) 443
TspGWI ACGGA 3 cut(s) 92, 468, 591
TspRI CASTG 2 cut(s) 159, 229
Tth111I GACNNNGTC 1 cut(s) 284
VpaK11BI GGWCC 3 cut(s) 12, 38, 206
XagI CCTNNNNNAGG 2 cut(s) 50, 815
XceI RCATGY 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
Zsp2I ATGCAT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.