pycom04g02060

Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0018504
Physical Location & Seq
Forward (+)
1841136 .. 1843372
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
ATGATGACCTGTAGTGAACTCGGCTTCTTCCCCAGAATCTGCACTGCCATTACAAGGCTTTCTCCTCCTCCTCCGCCTCCTCCTGCGGCCACAGTCCACCACCATACCTTCTCTTCCTTCTCTAATTTGGTACTTCTGTTCTGCTCATACAATCAGACCCAAAGAATCGACGTTTTTGTTGGTTTCGTTTGGAGTCACGAGTCTGGAGGATACAAGTGGAAGGAAATGCAGCAACAGAAATTTATTTGGGGATGGGTTTTCATCGCATCTGGCTCCCTTTTCTTTCTGGGTTTTCTCTACGCCGCCGTGATATCCAAGCTTCTTCCGCCTTCAGATAATCCTATCGTCTCCGCGATCCAAAGCGACCGGTACTATTGCTTCTTGGTGCCCTTGACTCTGCCGGTCCTCGTGGTAGCTATATATTTTCACTGGCTCAGCATGAAACTGTTCAAGCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-Y

Protein Analysis

154

Amino Acids

17.47

Weight (kDa)

8.98

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-Y PF15159 84 - 152 4.4e-21 Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018504)

Species Orthologous Gene IDs
malus_domestica MD04G1023900.v1.1
prunus_persica Prupe.1G174200_v2.0.a1 Prupe.1G174200_v2.0.a1 Prupe.1G174200_v2.0.a1
pyrus_communis pycom04g02060
rosa_chinensis RchiOBHm_Chr4g0411561
rosa_multiflora Rmu_sc0001868.1_g000003 Rmu_sc0006550.1_g000002
rosa_samantha Rh4BG165800
rosa_wichuraiana Rw4G014040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 385
AccII CGCG 1 cut(s) 353
AciI CCGC 5 cut(s) 74, 86, 303, 326, 351
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 2 cut(s) 132, 371
AfiI CCNNNNNNNGG 1 cut(s) 54
AgeI ACCGGT 1 cut(s) 366
AgsI TTSAA 1 cut(s) 451
AluBI AGCT 2 cut(s) 319, 416
AluI AGCT 2 cut(s) 319, 416
Alw26I GTCTC 1 cut(s) 352
AlwI GGATC 1 cut(s) 349
AlwNI CAGNNNCTG 1 cut(s) 39
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 239
AsiGI ACCGGT 1 cut(s) 366
Asp700I GAANNNNTTC 1 cut(s) 446
AspS9I GGNCC 1 cut(s) 403
AvaII GGWCC 1 cut(s) 403
BaeGI GKGCMC 1 cut(s) 390
BaeI ACNNNNGTAYC 2 cut(s) 122, 155
BanI GGYRCC 1 cut(s) 385
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
BauI CACGAG 2 cut(s) 197, 407
BbvI GCAGC 1 cut(s) 241
BccI CCATC 1 cut(s) 246
BceAI ACGGC 1 cut(s) 290
BciVI GTATCC 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 352
BfmI CTRYAG 1 cut(s) 10
BfuI GTATCC 1 cut(s) 203
BisI GCNGC 3 cut(s) 87, 230, 303
BlpI GCTNAGC 1 cut(s) 434
BlsI GCNGC 3 cut(s) 88, 231, 304
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 2 cut(s) 274, 387
BmsI GCATC 1 cut(s) 275
BpmI CTGGAG 1 cut(s) 225
Bpu1102I GCTNAGC 1 cut(s) 434
BsaWI WCCGGW 1 cut(s) 366
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse118I RCCGGY 2 cut(s) 366, 400
Bse1I ACTGG 1 cut(s) 434
BseGI GGATG 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMII CTCAG 1 cut(s) 448
BseNI ACTGG 1 cut(s) 434
BseRI GAGGAG 4 cut(s) 54, 57, 60, 69
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 1 cut(s) 241
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 353
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 1 cut(s) 89
BshNI GGYRCC 1 cut(s) 385
BshTI ACCGGT 1 cut(s) 366
BsiEI CGRYCG 1 cut(s) 367
BsiSI CCGG 2 cut(s) 367, 401
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 352
BsmBI CGTCTC 1 cut(s) 352
BsnI GGCC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 1 cut(s) 354
Bsp1720I GCTNAGC 1 cut(s) 434
BspACI CCGC 5 cut(s) 74, 86, 303, 326, 351
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 1 cut(s) 447
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 2 cut(s) 274, 387
BspPI GGATC 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 385
BsrFI RCCGGY 2 cut(s) 366, 400
BsrI ACTGG 1 cut(s) 434
BssAI RCCGGY 2 cut(s) 366, 400
BssMI GATC 1 cut(s) 354
BssSI CACGAG 2 cut(s) 197, 407
Bst2BI CACGAG 2 cut(s) 197, 407
Bst4CI ACNGT 2 cut(s) 94, 447
Bst6I CTCTTC 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 1 cut(s) 434
BstF5I GGATG 1 cut(s) 257
BstFNI CGCG 1 cut(s) 353
BstKTI GATC 1 cut(s) 357
BstMAI GTCTC 1 cut(s) 352
BstMBI GATC 1 cut(s) 354
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 325
BstNSI RCATGY 1 cut(s) 458
BstSFI CTRYAG 1 cut(s) 10
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 1 cut(s) 241
BsuI GTATCC 1 cut(s) 203
BsuRI GGCC 1 cut(s) 89
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 1 cut(s) 257
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 2 cut(s) 42, 427
Cac8I GCNNGC 1 cut(s) 456
CaiI CAGNNNCTG 1 cut(s) 39
Cfr10I RCCGGY 2 cut(s) 366, 400
Cfr13I GGNCC 1 cut(s) 403
Csp6I GTAC 2 cut(s) 131, 370
CspAI ACCGGT 1 cut(s) 366
CviAII CATG 3 cut(s) 439, 455, 459
CviJI RGCY 7 cut(s) 24, 58, 89, 273, 319, 416, 433
CviKI_1 RGCY 7 cut(s) 24, 58, 89, 273, 319, 416, 433
CviQI GTAC 2 cut(s) 131, 370
DdeI CTNAG 1 cut(s) 434
DpnI GATC 1 cut(s) 356
DpnII GATC 1 cut(s) 354
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
EciI GGCGGA 2 cut(s) 63, 315
Eco32I GATATC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 315
EcoRV GATATC 1 cut(s) 312
EcoT22I ATGCAT 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 352
FaeI CATG 3 cut(s) 442, 458, 462
FaiI YATR 7 cut(s) 105, 148, 419, 421, 440, 456, 460
FatI CATG 3 cut(s) 438, 454, 458
Fnu4HI GCNGC 3 cut(s) 87, 230, 303
FokI GGATG 1 cut(s) 264
Fsp4HI GCNGC 3 cut(s) 87, 230, 303
GluI GCNGC 3 cut(s) 87, 230, 303
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 1 cut(s) 89
HapII CCGG 2 cut(s) 367, 401
Hin1II CATG 3 cut(s) 442, 458, 462
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 5 cut(s) 36, 165, 193, 200, 394
HpaII CCGG 2 cut(s) 367, 401
Hpy166II GTNNAC 2 cut(s) 17, 97
Hpy188I TCNGA 2 cut(s) 156, 334
Hpy188III TCNNGA 2 cut(s) 197, 204
Hpy8I GTNNAC 2 cut(s) 17, 97
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 4 cut(s) 118, 127, 214, 339
HpyCH4III ACNGT 2 cut(s) 94, 447
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 42, 229, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 325
HpyF3I CTNAG 1 cut(s) 434
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 3 cut(s) 442, 458, 462
Kzo9I GATC 1 cut(s) 354
LmnI GCTCC 1 cut(s) 278
LpnPI CCDG 9 cut(s) 22, 46, 96, 189, 255, 272, 380, 414, 415
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 1 cut(s) 275
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 194
MalI GATC 1 cut(s) 356
MboI GATC 1 cut(s) 354
MboII GAAGA 3 cut(s) 19, 105, 314
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 2 cut(s) 124, 239
MlyI GAGTC 3 cut(s) 202, 209, 388
MnlI CCTC 7 cut(s) 75, 78, 81, 87, 90, 200, 416
Mph1103I ATGCAT 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 446
MspI CCGG 2 cut(s) 367, 401
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 1 cut(s) 325
NdeII GATC 1 cut(s) 354
NlaIII CATG 3 cut(s) 442, 458, 462
NlaIV GGNNCC 2 cut(s) 274, 387
NmuCI GTSAC 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 460
NspI RCATGY 1 cut(s) 458
PaeI GCATGC 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 2 cut(s) 36, 165
PinAI ACCGGT 1 cut(s) 366
PkrI GCNGC 3 cut(s) 88, 231, 304
PleI GAGTC 3 cut(s) 201, 208, 388
PpsI GAGTC 3 cut(s) 201, 208, 388
PspN4I GGNNCC 2 cut(s) 274, 387
PspPI GGNCC 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 39
RsaI GTAC 2 cut(s) 132, 371
RsaNI GTAC 2 cut(s) 131, 370
SatI GCNGC 3 cut(s) 87, 230, 303
Sau3AI GATC 1 cut(s) 354
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 3 cut(s) 202, 209, 388
SduI GDGCHC 1 cut(s) 390
SetI ASST 5 cut(s) 11, 110, 174, 321, 418
SfaNI GCATC 1 cut(s) 275
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 1 cut(s) 403
SphI GCATGC 1 cut(s) 458
Sse9I AATT 2 cut(s) 124, 239
SsiI CCGC 5 cut(s) 74, 86, 303, 326, 351
TaaI ACNGT 2 cut(s) 94, 447
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 168
TasI AATT 2 cut(s) 124, 239
TauI GCSGC 2 cut(s) 89, 305
TfiI GAWTC 2 cut(s) 36, 165
TscAI CASTG 2 cut(s) 49, 434
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 2 cut(s) 250, 455
TspRI CASTG 2 cut(s) 49, 434
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 1 cut(s) 239
XceI RCATGY 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 446
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.