pycom04g05070

poly(U)-specific endoribonuclease-B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012207
Physical Location & Seq
Reverse (-)
4717193 .. 4719784
2592 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05070.5

Sequence Viewer

Length: 1320 bp
ATGGAGGGTCTGATCAAGGGTCTGATCGACGTGGCGATCGGTCACGATGACAACAGGGACGAAGAACCCGGTCCACGGGACCGGGAGGAACAGTCCAGATCCACTTGGGCGCAGGTTGTGTCCGGGGAGGAGCAGGATGAGGGTGATCGTGATCGCCCACATGGGTTAAACCAAAATCAATGGAATAGACAGGAACAGAGTCATTTGGGGAACACAGGGGGATACAATGTGGATTCGGAACCTTCAAGGAGACCTCACAAGGCTTCGCATGAAGGATATGAAACGAATAATGATGGTGGACAACATGGTTATAACCAAAACTACTCGCGCAGGAAGGACGAAGAGGAAAATCAGGATCGTTGGGAGACTGTCGGCAAGAAGCCTCCAAGGCAACAACCACACAAGATTCAGATGGGCCAGTGGAATGGATATAAGCGGCCTGCCACTGAGCAAGAATACTCCGATGAGGCTGAAGTAGGTGCTAACATAGAACCTTCTGGAGAAGAGCTTTCTGATCTGTCACAAGCTTGCAATAAACTCTGGGAACTTGATTTAAACCGTCTAGTGCCTGGAAAGGACTATGAAATTGACTGTGGTGGAGGGAAGAAGGTCCACCAACGAGAAGACTTGGCAGAAGCAAGCTTATTTACCTGGCTGAGTGAAGATATATTTCGGAGGCCTACCTTTTCTCGTTTCTGTTCTCTTTTAGATAACTACAACCCAAATGCGGGTTGCAAAGAAGTTATCACATCCGAGGAGAAGCAAGAGCAAGCTGCATTCATAGAAGAAATCAGCAGAACTGCCCCCATTAAATATCTTCACAAGTATCTCTCACGTAAGGGCATTGTATCTGAGAGCTATCAGGAATTCAAAAGAACATTGTCCACTCTCTGGTTTGATCTTTACGGCCGTGGTGGTACATCTGCTTCCTCCTCGGCTTTTGAGCATGTTTTTGTCGGAGAAATTAAACAACGTGGTGAAGAAGAGGTTTCTGGCTTCCACAATTGGCTTCAGTTTTACCTTGAAGAAGCAAAAGGAAGGGTAGACTATCAAGGTTATATTTTTCCCCGTAGACGCGGACAAACTCCAGACTCTGAAACCCAGTTGCTTACGATCCAGTTCGAGTGGAATGGTGTTTTGAAAGCGGTATCAAGCACATTAGTTGGAGTGAGCCCAGAATTTGAACTTGCATTGTACACCTTGTGTTTCTACATGGGTGGGGAGGACAACCGTATTGAGCTGGGTCCATACCAAGTCAACATCAAATGCTATAGCTTTGGAGAAAGAATTGGGTCTGTGTTTCCCATGGCAGAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XendoU

Protein Analysis

440

Amino Acids

50.29

Weight (kDa)

4.99

Isoelectric Point (pI)

59.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17100
fragaria_vesca FvH4_5g20810 FvH4_5g20810
malus_domestica MD04G1057000.v1.1
prunus_persica Prupe.5G050800_v2.0.a1 Prupe.5G050800_v2.0.a1
pyrus_communis pycom04g05070
rosa_chinensis RchiOBHm_Chr7g0207741
rosa_laevigata RLG00000003291
rosa_multiflora Rmu_sc0005968.1_g000004 Rmu_sc0007645.1_g000005
rosa_roxburghii Rroxscaffold_3G00251190
rosa_rugosa Rorug07G0099300 Rorug07G0099400
rosa_samantha Rh7AG232500 Rh7BG227900 Rh7CG246200 Rh7DG238700
rosa_wichuraiana Rw7G019910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 103
AccB7I CCANNNNNTGG 1 cut(s) 891
AccI GTMKAC 2 cut(s) 1044, 1072
AccII CGCG 2 cut(s) 328, 1077
AciI CCGC 4 cut(s) 436, 728, 1077, 1145
AclWI GGATC 3 cut(s) 93, 363, 1108
AcoI YGGCCR 1 cut(s) 907
AcsI RAATTY 2 cut(s) 866, 1178
AcuI CTGAAG 2 cut(s) 492, 995
AdeI CACNNNGTG 1 cut(s) 1203
AfaI GTAC 2 cut(s) 919, 1196
AfiI CCNNNNNNNGG 4 cut(s) 75, 727, 728, 891
AgsI TTSAA 5 cut(s) 246, 871, 1025, 1141, 1184
AjiI CACGTC 1 cut(s) 31
AjnI CCWGG 2 cut(s) 568, 650
AluBI AGCT 7 cut(s) 508, 527, 642, 773, 858, 1240, 1275
AluI AGCT 7 cut(s) 508, 527, 642, 773, 858, 1240, 1275
Alw26I GTCTC 2 cut(s) 244, 359
AlwI GGATC 3 cut(s) 93, 363, 1108
AlwNI CAGNNNCTG 1 cut(s) 1094
AoxI GGCC 4 cut(s) 415, 437, 677, 907
ApeKI GCWGC 1 cut(s) 773
ApoI RAATTY 2 cut(s) 866, 1178
AspLEI GCGC 2 cut(s) 112, 330
AspS9I GGNCC 5 cut(s) 71, 79, 415, 610, 1244
AsuC2I CCSGG 3 cut(s) 69, 83, 124
AsuHPI GGTGA 2 cut(s) 155, 989
AvaII GGWCC 4 cut(s) 71, 79, 610, 1244
BanII GRGCYC 1 cut(s) 1175
BarI GAAGNNNNNNTAC 2 cut(s) 910, 942
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 760
BccI CCATC 2 cut(s) 287, 406
BceAI ACGGC 2 cut(s) 894, 922
BciT130I CCWGG 2 cut(s) 570, 652
BciVI GTATCC 1 cut(s) 215
BclI TGATCA 1 cut(s) 12
BcnI CCSGG 3 cut(s) 69, 83, 124
BcoDI GTCTC 2 cut(s) 244, 359
BfaI CTAG 1 cut(s) 563
BfmI CTRYAG 1 cut(s) 1270
BfuAI ACCTGC 1 cut(s) 103
BfuI GTATCC 1 cut(s) 215
BglI GCCNNNNNGGC 1 cut(s) 388
BisI GCNGC 2 cut(s) 437, 774
BlsI GCNGC 2 cut(s) 438, 775
Bme1390I CCNGG 5 cut(s) 69, 83, 124, 570, 652
Bme18I GGWCC 4 cut(s) 71, 79, 610, 1244
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 5 cut(s) 71, 79, 415, 610, 1244
BmiI GGNNCC 3 cut(s) 80, 240, 1245
BmrFI CCNGG 5 cut(s) 69, 83, 124, 570, 652
BmrI ACTGGG 1 cut(s) 1096
BmuI ACTGGG 1 cut(s) 1096
BpiI GAAGAC 1 cut(s) 630
BpmI CTGGAG 2 cut(s) 519, 1071
BpuMI CCSGG 3 cut(s) 69, 83, 124
BsaAI YACGTR 1 cut(s) 836
BsaBI GATNNNNATC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 7 cut(s) 74, 123, 386, 753, 910, 933, 1305
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 727, 728, 891
Bse1I ACTGG 3 cut(s) 418, 1102, 1117
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 2 cut(s) 570, 652
BseDI CCNNGG 7 cut(s) 74, 123, 386, 753, 910, 933, 1305
BseGI GGATG 2 cut(s) 142, 749
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 4 cut(s) 75, 727, 728, 891
BseMII CTCAG 3 cut(s) 438, 647, 843
BseNI ACTGG 3 cut(s) 418, 1102, 1117
BseRI GAGGAG 3 cut(s) 143, 770, 922
BseX3I CGGCCG 1 cut(s) 907
BseXI GCAGC 1 cut(s) 760
BseYI CCCAGC 1 cut(s) 1240
Bsh1236I CGCG 2 cut(s) 328, 1077
Bsh1285I CGRYCG 2 cut(s) 39, 910
BshFI GGCC 4 cut(s) 417, 439, 679, 909
BsiEI CGRYCG 2 cut(s) 39, 910
BsiSI CCGG 3 cut(s) 69, 82, 123
BslFI GGGAC 2 cut(s) 71, 92
BslI CCNNNNNNNGG 4 cut(s) 75, 727, 728, 891
BsmAI GTCTC 2 cut(s) 244, 359
BsmFI GGGAC 2 cut(s) 71, 92
BsmI GAATGC 1 cut(s) 776
BsnI GGCC 4 cut(s) 417, 439, 679, 909
Bso31I GGTCTC 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 1175
Bsp1407I TGTACA 1 cut(s) 1194
Bsp19I CCATGG 1 cut(s) 1305
BspACI CCGC 4 cut(s) 436, 728, 1077, 1145
BspANI GGCC 4 cut(s) 417, 439, 679, 909
BspCNI CTCAG 3 cut(s) 439, 648, 844
BspFNI CGCG 2 cut(s) 328, 1077
BspLI GGNNCC 3 cut(s) 80, 240, 1245
BspMI ACCTGC 1 cut(s) 103
BspPI GGATC 3 cut(s) 93, 363, 1108
BspQI GCTCTTC 1 cut(s) 498
BspTNI GGTCTC 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 1194
BsrI ACTGG 3 cut(s) 418, 1102, 1117
BssECI CCNNGG 7 cut(s) 74, 123, 386, 753, 910, 933, 1305
BssT1I CCWWGG 2 cut(s) 386, 1305
Bst2UI CCWGG 2 cut(s) 570, 652
Bst4CI ACNGT 5 cut(s) 93, 370, 560, 593, 1232
Bst6I CTCTTC 3 cut(s) 336, 498, 978
BstAUI TGTACA 1 cut(s) 1194
BstBAI YACGTR 1 cut(s) 836
BstC8I GCNNGC 4 cut(s) 441, 529, 640, 771
BstDEI CTNAG 3 cut(s) 447, 656, 852
BstDSI CCRYGG 3 cut(s) 74, 910, 1305
BstF5I GGATG 2 cut(s) 142, 749
BstFNI CGCG 2 cut(s) 328, 1077
BstHHI GCGC 2 cut(s) 112, 330
BstMAI GTCTC 2 cut(s) 244, 359
BstMCI CGRYCG 2 cut(s) 39, 910
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 2 cut(s) 570, 652
BstNSI RCATGY 1 cut(s) 950
BstSCI CCNGG 5 cut(s) 67, 81, 122, 568, 650
BstSFI CTRYAG 1 cut(s) 1270
BstUI CGCG 2 cut(s) 328, 1077
BstV1I GCAGC 1 cut(s) 760
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 1 cut(s) 98
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 1 cut(s) 98
BstZI CGGCCG 1 cut(s) 907
BsuI GTATCC 1 cut(s) 215
BsuRI GGCC 4 cut(s) 417, 439, 679, 909
BtgI CCRYGG 3 cut(s) 74, 910, 1305
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 2 cut(s) 142, 749
BtsIMutI CAGTG 2 cut(s) 425, 444
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 4 cut(s) 441, 529, 640, 771
CaiI CAGNNNCTG 1 cut(s) 1094
CfoI GCGC 2 cut(s) 112, 330
Cfr13I GGNCC 5 cut(s) 71, 79, 415, 610, 1244
CseI GACGC 1 cut(s) 1083
Csp6I GTAC 2 cut(s) 918, 1195
CviAII CATG 6 cut(s) 161, 269, 305, 947, 1213, 1306
CviQI GTAC 2 cut(s) 918, 1195
DdeI CTNAG 3 cut(s) 447, 656, 852
DraI TTTAAA 1 cut(s) 555
DraIII CACNNNGTG 1 cut(s) 1203
EaeI YGGCCR 1 cut(s) 907
EagI CGGCCG 1 cut(s) 907
Eam1104I CTCTTC 3 cut(s) 336, 498, 978
EarI CTCTTC 3 cut(s) 336, 498, 978
EclXI CGGCCG 1 cut(s) 907
Eco130I CCWWGG 2 cut(s) 386, 1305
Eco147I AGGCCT 1 cut(s) 679
Eco24I GRGCYC 1 cut(s) 1175
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 4 cut(s) 71, 79, 610, 1244
Eco52I CGGCCG 1 cut(s) 907
Eco57I CTGAAG 2 cut(s) 492, 995
EcoRI GAATTC 1 cut(s) 866
EcoRII CCWGG 2 cut(s) 568, 650
EcoT14I CCWWGG 2 cut(s) 386, 1305
EcoT38I GRGCYC 1 cut(s) 1175
ErhI CCWWGG 2 cut(s) 386, 1305
FaeI CATG 6 cut(s) 164, 272, 308, 950, 1216, 1309
FaqI GGGAC 2 cut(s) 71, 92
FatI CATG 6 cut(s) 160, 268, 304, 946, 1212, 1305
FauI CCCGC 1 cut(s) 721
FbaI TGATCA 1 cut(s) 12
FblI GTMKAC 2 cut(s) 1044, 1072
Fnu4HI GCNGC 2 cut(s) 437, 774
FokI GGATG 2 cut(s) 149, 736
FriOI GRGCYC 1 cut(s) 1175
Fsp4HI GCNGC 2 cut(s) 437, 774
FspBI CTAG 1 cut(s) 563
GlaI GCGC 2 cut(s) 111, 329
GluI GCNGC 2 cut(s) 437, 774
GsaI CCCAGC 1 cut(s) 1244
GsuI CTGGAG 2 cut(s) 519, 1071
HaeIII GGCC 4 cut(s) 417, 439, 679, 909
HapII CCGG 3 cut(s) 69, 82, 123
HgaI GACGC 1 cut(s) 1083
HhaI GCGC 2 cut(s) 112, 330
Hin1II CATG 6 cut(s) 164, 272, 308, 950, 1216, 1309
Hin6I GCGC 2 cut(s) 110, 328
HinP1I GCGC 2 cut(s) 110, 328
HincII GTYRAC 1 cut(s) 1258
HindII GTYRAC 1 cut(s) 1258
HindIII AAGCTT 2 cut(s) 525, 640
HinfI GANTC 4 cut(s) 199, 233, 406, 1091
HpaII CCGG 3 cut(s) 69, 82, 123
HphI GGTGA 2 cut(s) 155, 989
Hpy166II GTNNAC 8 cut(s) 74, 299, 613, 885, 1045, 1073, 1197, 1258
Hpy188III TCNNGA 7 cut(s) 44, 96, 149, 353, 498, 863, 1088
Hpy8I GTNNAC 8 cut(s) 74, 299, 613, 885, 1045, 1073, 1197, 1258
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 6 cut(s) 252, 266, 328, 504, 601, 1032
HpyCH4III ACNGT 5 cut(s) 93, 370, 560, 593, 1232
HpyCH4IV ACGT 3 cut(s) 30, 835, 973
HpyCH4V TGCA 4 cut(s) 531, 735, 776, 1190
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 3 cut(s) 447, 656, 852
HpySE526I ACGT 3 cut(s) 30, 835, 973
Hsp92II CATG 6 cut(s) 164, 272, 308, 950, 1216, 1309
HspAI GCGC 2 cut(s) 110, 328
Ksp22I TGATCA 1 cut(s) 12
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 1 cut(s) 130
Lsp1109I GCAGC 1 cut(s) 760
MaeI CTAG 1 cut(s) 563
MaeII ACGT 3 cut(s) 30, 835, 973
MaeIII GTNAC 2 cut(s) 41, 519
MfeI CAATTG 1 cut(s) 1003
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 1 cut(s) 1175
MluCI AATT 6 cut(s) 585, 866, 963, 1003, 1178, 1287
MlyI GAGTC 2 cut(s) 208, 1085
MmeI TCCRAC 2 cut(s) 937, 1144
MseI TTAA 4 cut(s) 167, 554, 810, 966
MspI CCGG 3 cut(s) 69, 82, 123
MspR9I CCNGG 5 cut(s) 69, 83, 124, 570, 652
MunI CAATTG 1 cut(s) 1003
Mva1269I GAATGC 1 cut(s) 776
MvaI CCWGG 2 cut(s) 570, 652
MvnI CGCG 2 cut(s) 328, 1077
MwoI GCNNNNNNNGC 1 cut(s) 388
NciI CCSGG 3 cut(s) 69, 83, 124
NcoI CCATGG 1 cut(s) 1305
NlaIII CATG 6 cut(s) 164, 272, 308, 950, 1216, 1309
NlaIV GGNNCC 3 cut(s) 80, 240, 1245
NmeAIII GCCGAG 1 cut(s) 914
NmuCI GTSAC 2 cut(s) 41, 519
NspI RCATGY 1 cut(s) 950
PceI AGGCCT 1 cut(s) 679
PciSI GCTCTTC 1 cut(s) 498
PctI GAATGC 1 cut(s) 776
PfeI GAWTC 2 cut(s) 233, 406
PflMI CCANNNNNTGG 1 cut(s) 891
PkrI GCNGC 2 cut(s) 438, 775
Ple19I CGATCG 1 cut(s) 39
PleI GAGTC 2 cut(s) 207, 1085
PpsI GAGTC 2 cut(s) 207, 1085
Ppu21I YACGTR 1 cut(s) 836
PsiI TTATAA 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 568, 650
PspFI CCCAGC 1 cut(s) 1240
PspGI CCWGG 2 cut(s) 568, 650
PspN4I GGNNCC 3 cut(s) 80, 240, 1245
PspPI GGNCC 5 cut(s) 71, 79, 415, 610, 1244
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 1 cut(s) 98
PvuI CGATCG 1 cut(s) 39
RsaI GTAC 2 cut(s) 919, 1196
RsaNI GTAC 2 cut(s) 918, 1195
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 4 cut(s) 167, 554, 810, 966
SatI GCNGC 2 cut(s) 437, 774
Sau96I GGNCC 5 cut(s) 71, 79, 415, 610, 1244
SchI GAGTC 2 cut(s) 208, 1085
ScrFI CCNGG 5 cut(s) 69, 83, 124, 570, 652
SduI GDGCHC 1 cut(s) 1175
SfcI CTRYAG 1 cut(s) 1270
SinI GGWCC 4 cut(s) 71, 79, 610, 1244
Sse9I AATT 6 cut(s) 585, 866, 963, 1003, 1178, 1287
SseBI AGGCCT 1 cut(s) 679
SsiI CCGC 4 cut(s) 436, 728, 1077, 1145
SspMI CTAG 1 cut(s) 563
StuI AGGCCT 1 cut(s) 679
StyD4I CCNGG 5 cut(s) 67, 81, 122, 568, 650
StyI CCWWGG 2 cut(s) 386, 1305
TaaI ACNGT 5 cut(s) 93, 370, 560, 593, 1232
TaiI ACGT 3 cut(s) 33, 838, 976
TaqI TCGA 2 cut(s) 27, 1122
TaqII GACCGA 1 cut(s) 29
TasI AATT 6 cut(s) 585, 866, 963, 1003, 1178, 1287
TatI WGTACW 1 cut(s) 1194
TauI GCSGC 1 cut(s) 439
TfiI GAWTC 2 cut(s) 233, 406
Tru1I TTAA 4 cut(s) 167, 554, 810, 966
Tru9I TTAA 4 cut(s) 167, 554, 810, 966
TscAI CASTG 2 cut(s) 425, 451
TseFI GTSAC 2 cut(s) 41, 519
TseI GCWGC 1 cut(s) 773
Tsp45I GTSAC 2 cut(s) 41, 519
TspDTI ATGAA 4 cut(s) 285, 294, 597, 769
TspRI CASTG 2 cut(s) 425, 451
Van91I CCANNNNNTGG 1 cut(s) 891
VpaK11BI GGWCC 4 cut(s) 71, 79, 610, 1244
XapI RAATTY 2 cut(s) 866, 1178
XceI RCATGY 1 cut(s) 950
XmiI GTMKAC 2 cut(s) 1044, 1072
XspI CTAG 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.