Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0016171

Physical Location & Seq

Genomic Coordinates Forward (+)

8082743 .. 8086814

Gene Structure 4072 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGACCAAAATCCAGTTCTCAACATTGTACTCTATAAACCTCATCTTCCTCACCCCCCGACTCTTCCCCGCGAGCCTCCGAAAAATGGTCGTCTCACTAAGACTCCCATCTCTGAGCGCGCTCTTCTCTCCCGCCATCTCCCGCCGTCCTTCGTCACTTCTCCGCTGTAACCACCACCGCGTACGAAGACCCGTATGCTCCGCCTCTTTCTCTGCTCAGAGCACGGTGTCGGAAACGAATGGCCGAGTCGACACTCAGACTCAGAGCAGCAACCGAGTCGTGGACCGAGCCGTCACTCCTCGGTCAGAGGACTTCAATGCTTGGTACCTCGATGTCATTGAGCAAGCAGAGCTCGCCGATTACGGTCCGGTTCGGGGCACCATGGTCATCCGTCCCTACGGTTACGCCATATGGGAAGCTATTCAGGATTATCTGAATGTGAAATTTAAAGAGACTGGCCACAGTAACATGTATTTCCCACAGTTTATACCGTATTCGTTTATAGAGAAAGAAGCTAGCCATGTTGAAGGGTTTAGTCCCGAATTAGCAGTTGTGACAATAGGAGGAGGGAAGGAGCTTGAAGAAAAACTTGTGGTTCGACCCACAAGTGAAACCATAGTAAATCACATGTTCACTCAGTGGATTCATAGTTACCGTGATCTTCCCCTTATGGTTAACCAATGGGCGAATGTGACAAGATGGGAGATGCGGACGAAACCTTTTGTGAGGACACTTGAATTTCTCTGGCAGGAGGGTCATACGGCTCATGCTACTCCAGAGGAGGCAGAGAAAGAGGCTATACAGATGATTGACGTGTATACAAAATTTGCTTATGAGCAAACTGCAATACCCGTTATTGCAGGTCGAAAGTCAAAAGTTGAAACATTTGCTGGTGCTGTTAGGACCTATACAATAGAGGCTATGATGGGTGATCGGAAGGCATTACAGGCTGGCACCAGCCACAATCTTGGGCAGAATTTTTCTCGTGCCTTTCAAACACAGTTTACTGATGAAAGTGGACAGAGGCAACACGTGTGGCAGACATCGTGGGCAGTTAGTACCCGTTTTGTTGGTGGTATTATCATGACCCATGGAGACGATACAGGTTTAATGCTTCCCCCAAAGATTGCACCAATACAGGTGGTGATCACACCAATTTGGAAGAAGGATGACGATAGAGAAGGAGTTCTGAATGCTGCATCATCTGTAAAAGAAGCATTGCAGACTGCAGGCATTAGAGTTAAACTCGATGACTCTGATCAGAGAACCCCAGGATGGAAATTCAATTTCTGGGAGATGAAGGGAGTCCCTGTAAGAATTGAAATTGGCCCTCGTGATGTTTCGAGCCAGACTGTGGTTATATGTAGAAGAGATGTTCCTGGAAAGCAAGGGAAGGTCTTCGGAATATCCATGGAGCCCTCAATTTTGGAGGCTCATATCAAGGACAAGTTGGATGAGATTCAGTCATCACTTTTGGAAAAAGCAAAATCATTCCGAGATTGTAATATTGTTGATGTCAGCTCATATGATGAACTCAAAGTTGCAATCTCCCAGGGAAAATGGGCAAGAGGTCCTTGGTCGGCTAGTGATGAAGATGAGTTAAAGGTAAAAGAAGAAACAGGGGCAACCATTAGGTGTTTTCCTTTTGAACAGCCGCAAGATATTAAAACATGCTTGATGACTGGTAATCCAGCGGAGGAAGTAGCTATATTTGCAAAGTCATACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004827 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006433 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009570 GO:0009791 GO:0009908 GO:0009987 GO:0010109 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0022414 GO:0031323 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0035670 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0071704 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon ProRS-C_1 tRNA-synt_2b

Protein Analysis

576

Amino Acids

64.96

Weight (kDa)

6.89

Isoelectric Point (pI)

37.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2b	PF00587	192 - 361	7e-21	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	380 - 460	1.6e-16	Anticodon binding domain
ProRS-C_1	PF09180	509 - 575	6.1e-20	Prolyl-tRNA synthetase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52520
fragaria_vesca	FvH4_5g00340 FvH4_5g00340
prunus_persica	Prupe.5G076000_v2.0.a1
pyrus_communis	pycom04g07460
rosa_chinensis	RchiOBHm_Chr7g0202211
rosa_laevigata	RLG00000003616
rosa_multiflora	Rmu_sc0005017.1_g000003
rosa_roxburghii	Rroxscaffold_3G00254740
rosa_rugosa	Rorug07G0069000
rosa_samantha	Rh7AG196400 Rh7BG197600 Rh7CG207500 Rh7DG203000
rosa_wichuraiana	Rw7G017190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	290
Acc36I	ACCTGC	1 cut(s)	851
Acc65I	GGTACC	1 cut(s)	324
AccB1I	GGYRCC	3 cut(s)	324, 377, 953
AccB7I	CCANNNNNTGG	1 cut(s)	1354
AccI	GTMKAC	2 cut(s)	249, 818
AccII	CGCG	3 cut(s)	71, 119, 180
AciI	CCGC	9 cut(s)	69, 132, 142, 163, 178, 201, 709, 1655, 1694
AcoI	YGGCCR	2 cut(s)	241, 457
AcsI	RAATTY	5 cut(s)	443, 737, 824, 976, 1280
AcvI	CACGTG	1 cut(s)	1033
AdeI	CACNNNGTG	1 cut(s)	639
AfaI	GTAC	4 cut(s)	29, 183, 326, 1060
AfiI	CCNNNNNNNGG	5 cut(s)	85, 280, 374, 1275, 1354
AflIII	ACRYGT	5 cut(s)	468, 627, 813, 1030, 1032
AgsI	TTSAA	9 cut(s)	316, 527, 581, 737, 881, 995, 1285, 1322, 1649
AjiI	CACGTC	1 cut(s)	814
AjnI	CCWGG	3 cut(s)	1270, 1378, 1551
AjuI	GAANNNNNNNTTGG	1 cut(s)	31
AluBI	AGCT	6 cut(s)	352, 419, 515, 577, 1521, 1706
AluI	AGCT	6 cut(s)	352, 419, 515, 577, 1521, 1706
Alw21I	GWGCWC	2 cut(s)	224, 354
Alw26I	GTCTC	3 cut(s)	97, 446, 1089
AoxI	GGCC	3 cut(s)	241, 457, 1327
ApeKI	GCWGC	2 cut(s)	267, 1196
ApoI	RAATTY	5 cut(s)	443, 737, 824, 976, 1280
Asp700I	GAANNNNTTC	3 cut(s)	420, 1185, 1397
Asp718I	GGTACC	1 cut(s)	324
AspLEI	GCGC	2 cut(s)	119, 121
AspS9I	GGNCC	5 cut(s)	283, 365, 903, 1328, 1571
AsuHPI	GGTGA	3 cut(s)	43, 941, 1156
AsuNHI	GCTAGC	1 cut(s)	515
AvaII	GGWCC	4 cut(s)	283, 365, 903, 1571
BaeGI	GKGCMC	1 cut(s)	380
BalI	TGGCCA	1 cut(s)	459
BanI	GGYRCC	3 cut(s)	324, 377, 953
BanII	GRGCYC	2 cut(s)	354, 1419
BauI	CACGAG	2 cut(s)	984, 1332
BbrPI	CACGTG	1 cut(s)	1033
BbsI	GAAGAC	2 cut(s)	193, 1390
Bbv12I	GWGCWC	2 cut(s)	224, 354
BbvI	GCAGC	2 cut(s)	279, 1183
BccI	CCATC	5 cut(s)	115, 143, 693, 919, 1269
BceAI	ACGGC	3 cut(s)	129, 275, 777
BcgI	CGANNNNNNTGC	2 cut(s)	259, 293
BciT130I	CCWGG	3 cut(s)	1272, 1380, 1553
BclI	TGATCA	2 cut(s)	1146, 1258
BcoDI	GTCTC	3 cut(s)	97, 446, 1089
BfaI	CTAG	2 cut(s)	516, 1584
BfmI	CTRYAG	1 cut(s)	1227
BfuAI	ACCTGC	1 cut(s)	851
BisI	GCNGC	3 cut(s)	268, 1197, 1655
BlsI	GCNGC	3 cut(s)	269, 1198, 1656
Bme1390I	CCNGG	3 cut(s)	1272, 1380, 1553
Bme18I	GGWCC	4 cut(s)	283, 365, 903, 1571
BmgBI	CACGTC	1 cut(s)	814
BmgT120I	GGNCC	5 cut(s)	283, 365, 903, 1328, 1571
BmiI	GGNNCC	4 cut(s)	326, 379, 955, 1416
BmrFI	CCNGG	3 cut(s)	1272, 1380, 1553
BmsI	GCATC	2 cut(s)	696, 1208
BmtI	GCTAGC	1 cut(s)	519
BpiI	GAAGAC	2 cut(s)	193, 1390
BplI	GAGNNNNNCTC	2 cut(s)	1230, 1262
BpmI	CTGGAG	1 cut(s)	759
BsaAI	YACGTR	1 cut(s)	1033
BsaJI	CCNNGG	8 cut(s)	299, 381, 1090, 1270, 1410, 1551, 1552, 1574
BsaWI	WCCGGW	1 cut(s)	367
BsaXI	ACNNNNNCTCC	4 cut(s)	87, 117, 1296, 1326
Bsc4I	CCNNNNNNNGG	5 cut(s)	85, 280, 374, 1275, 1354
Bse1I	ACTGG	3 cut(s)	13, 460, 1687
Bse3DI	GCAATG	1 cut(s)	1217
BseBI	CCWGG	3 cut(s)	1272, 1380, 1553
BseDI	CCNNGG	8 cut(s)	299, 381, 1090, 1270, 1410, 1551, 1552, 1574
BseGI	GGATG	4 cut(s)	387, 1174, 1280, 1459
BseLI	CCNNNNNNNGG	5 cut(s)	85, 280, 374, 1275, 1354
BseMI	GCAATG	1 cut(s)	1217
BseMII	CTCAG	5 cut(s)	104, 230, 269, 275, 650
BseNI	ACTGG	3 cut(s)	13, 460, 1687
BsePI	GCGCGC	1 cut(s)	117
BseRI	GAGGAG	3 cut(s)	288, 579, 794
BseSI	GKGCMC	1 cut(s)	380
BseXI	GCAGC	2 cut(s)	279, 1183
Bsh1236I	CGCG	3 cut(s)	71, 119, 180
BshFI	GGCC	3 cut(s)	243, 459, 1329
BshNI	GGYRCC	3 cut(s)	324, 377, 953
BsiHKAI	GWGCWC	2 cut(s)	224, 354
BsiSI	CCGG	1 cut(s)	368
BsiWI	CGTACG	1 cut(s)	181
BslFI	GGGAC	3 cut(s)	378, 522, 1292
BslI	CCNNNNNNNGG	5 cut(s)	85, 280, 374, 1275, 1354
BsmAI	GTCTC	3 cut(s)	97, 446, 1089
BsmBI	CGTCTC	2 cut(s)	97, 1089
BsmFI	GGGAC	3 cut(s)	378, 522, 1292
BsmI	GAATGC	1 cut(s)	1198
BsnI	GGCC	3 cut(s)	243, 459, 1329
Bsp1286I	GDGCHC	4 cut(s)	224, 354, 380, 1419
Bsp143I	GATC	4 cut(s)	658, 931, 1146, 1258
Bsp19I	CCATGG	3 cut(s)	381, 1090, 1410
BspACI	CCGC	9 cut(s)	69, 132, 142, 163, 178, 201, 709, 1655, 1694
BspANI	GGCC	3 cut(s)	243, 459, 1329
BspCNI	CTCAG	5 cut(s)	105, 229, 268, 274, 649
BspFNI	CGCG	3 cut(s)	71, 119, 180
BspHI	TCATGA	1 cut(s)	1083
BspLI	GGNNCC	4 cut(s)	326, 379, 955, 1416
BspMAI	CTGCAG	1 cut(s)	1231
BspMI	ACCTGC	1 cut(s)	851
BspOI	GCTAGC	1 cut(s)	519
BspQI	GCTCTTC	1 cut(s)	128
BspT107I	GGYRCC	3 cut(s)	324, 377, 953
BsrDI	GCAATG	1 cut(s)	1217
BsrI	ACTGG	3 cut(s)	13, 460, 1687
BssECI	CCNNGG	8 cut(s)	299, 381, 1090, 1270, 1410, 1551, 1552, 1574
BssHII	GCGCGC	1 cut(s)	117
BssMI	GATC	4 cut(s)	658, 931, 1146, 1258
BssNAI	GTATAC	1 cut(s)	819
BssSI	CACGAG	2 cut(s)	984, 1332
BssT1I	CCWWGG	4 cut(s)	381, 1090, 1410, 1574
Bst1107I	GTATAC	1 cut(s)	819
Bst2BI	CACGAG	2 cut(s)	984, 1332
Bst2UI	CCWGG	3 cut(s)	1272, 1380, 1553
Bst4CI	ACNGT	9 cut(s)	226, 365, 401, 464, 483, 492, 656, 1002, 1354
Bst6I	CTCTTC	3 cut(s)	68, 128, 1363
BstBAI	YACGTR	1 cut(s)	1033
BstC8I	GCNNGC	7 cut(s)	73, 119, 345, 354, 517, 952, 1231
BstDEI	CTNAG	6 cut(s)	98, 113, 216, 255, 261, 636
BstDSI	CCRYGG	3 cut(s)	381, 1090, 1410
BstF5I	GGATG	4 cut(s)	387, 1174, 1280, 1459
BstFNI	CGCG	3 cut(s)	71, 119, 180
BstHHI	GCGC	2 cut(s)	119, 121
BstKTI	GATC	4 cut(s)	661, 934, 1149, 1261
BstMAI	GTCTC	3 cut(s)	97, 446, 1089
BstMBI	GATC	4 cut(s)	658, 931, 1146, 1258
BstMWI	GCNNNNNNNGC	4 cut(s)	349, 353, 947, 1712
BstNI	CCWGG	3 cut(s)	1272, 1380, 1553
BstNSI	RCATGY	3 cut(s)	472, 631, 1674
BstSCI	CCNGG	3 cut(s)	1270, 1378, 1551
BstSFI	CTRYAG	1 cut(s)	1227
BstSLI	GKGCMC	1 cut(s)	380
BstUI	CGCG	3 cut(s)	71, 119, 180
BstV1I	GCAGC	2 cut(s)	279, 1183
BstV2I	GAAGAC	2 cut(s)	193, 1390
BstXI	CCANNNNNNTGG	1 cut(s)	968
BstZ17I	GTATAC	1 cut(s)	819
BsuRI	GGCC	3 cut(s)	243, 459, 1329
BtgI	CCRYGG	3 cut(s)	381, 1090, 1410
BtrI	CACGTC	1 cut(s)	814
BtsCI	GGATG	4 cut(s)	387, 1174, 1280, 1459
BtsIMutI	CAGTG	1 cut(s)	644
BveI	ACCTGC	1 cut(s)	851
Cac8I	GCNNGC	7 cut(s)	73, 119, 345, 354, 517, 952, 1231
CciI	TCATGA	1 cut(s)	1083
CfoI	GCGC	2 cut(s)	119, 121
Cfr13I	GGNCC	5 cut(s)	283, 365, 903, 1328, 1571
CpoI	CGGWCCG	1 cut(s)	365
Csp6I	GTAC	4 cut(s)	28, 182, 325, 1059
CspCI	CAANNNNNGTGG	4 cut(s)	1016, 1051, 1122, 1157
CspI	CGGWCCG	1 cut(s)	365
CviAII	CATG	9 cut(s)	382, 469, 521, 628, 767, 1084, 1091, 1411, 1671
CviQI	GTAC	4 cut(s)	28, 182, 325, 1059
DdeI	CTNAG	6 cut(s)	98, 113, 216, 255, 261, 636
DpnI	GATC	4 cut(s)	660, 933, 1148, 1260
DpnII	GATC	4 cut(s)	658, 931, 1146, 1258
DraI	TTTAAA	1 cut(s)	448
DraIII	CACNNNGTG	1 cut(s)	639
DrdI	GACNNNNNNGTC	1 cut(s)	290
DseDI	GACNNNNNNGTC	1 cut(s)	290
EaeI	YGGCCR	2 cut(s)	241, 457
Eam1104I	CTCTTC	3 cut(s)	68, 128, 1363
EarI	CTCTTC	3 cut(s)	68, 128, 1363
EciI	GGCGGA	1 cut(s)	190
Ecl136II	GAGCTC	1 cut(s)	352
Eco130I	CCWWGG	4 cut(s)	381, 1090, 1410, 1574
Eco24I	GRGCYC	2 cut(s)	354, 1419
Eco47I	GGWCC	4 cut(s)	283, 365, 903, 1571
Eco53kI	GAGCTC	1 cut(s)	352
Eco72I	CACGTG	1 cut(s)	1033
EcoICRI	GAGCTC	1 cut(s)	352
EcoO109I	RGGNCCY	2 cut(s)	903, 1571
EcoRII	CCWGG	3 cut(s)	1270, 1378, 1551
EcoT14I	CCWWGG	4 cut(s)	381, 1090, 1410, 1574
EcoT38I	GRGCYC	2 cut(s)	354, 1419
ErhI	CCWWGG	4 cut(s)	381, 1090, 1410, 1574
Esp3I	CGTCTC	2 cut(s)	97, 1089
FaeI	CATG	9 cut(s)	385, 472, 524, 631, 770, 1087, 1094, 1414, 1674
FalI	AAGNNNNNCTT	4 cut(s)	573, 605, 1558, 1590
FaqI	GGGAC	3 cut(s)	378, 522, 1292
FatI	CATG	9 cut(s)	381, 468, 520, 627, 766, 1083, 1090, 1410, 1670
FauI	CCCGC	3 cut(s)	76, 139, 149
FauNDI	CATATG	2 cut(s)	410, 1525
FbaI	TGATCA	2 cut(s)	1146, 1258
FblI	GTMKAC	2 cut(s)	249, 818
Fnu4HI	GCNGC	3 cut(s)	268, 1197, 1655
FokI	GGATG	4 cut(s)	374, 1181, 1287, 1466
FriOI	GRGCYC	2 cut(s)	354, 1419
Fsp4HI	GCNGC	3 cut(s)	268, 1197, 1655
FspBI	CTAG	2 cut(s)	516, 1584
GlaI	GCGC	2 cut(s)	118, 120
GluI	GCNGC	3 cut(s)	268, 1197, 1655
GsuI	CTGGAG	1 cut(s)	759
HaeIII	GGCC	3 cut(s)	243, 459, 1329
HapII	CCGG	1 cut(s)	368
HhaI	GCGC	2 cut(s)	119, 121
Hin1II	CATG	9 cut(s)	385, 472, 524, 631, 770, 1087, 1094, 1414, 1674
Hin6I	GCGC	2 cut(s)	117, 119
HinP1I	GCGC	2 cut(s)	117, 119
HincII	GTYRAC	2 cut(s)	250, 676
HindII	GTYRAC	2 cut(s)	250, 676
HinfI	GANTC	9 cut(s)	60, 102, 246, 259, 276, 643, 1253, 1305, 1459
HpaI	GTTAAC	1 cut(s)	676
HpaII	CCGG	1 cut(s)	368
HphI	GGTGA	3 cut(s)	43, 941, 1156
Hpy166II	GTNNAC	7 cut(s)	250, 283, 633, 676, 819, 1005, 1019
Hpy188III	TCNNGA	5 cut(s)	425, 539, 776, 1084, 1334
Hpy8I	GTNNAC	7 cut(s)	250, 283, 633, 676, 819, 1005, 1019
HpyAV	CCTTC	8 cut(s)	159, 521, 565, 931, 1159, 1175, 1294, 1387
HpyCH4III	ACNGT	9 cut(s)	226, 365, 401, 464, 483, 492, 656, 1002, 1354
HpyCH4IV	ACGT	2 cut(s)	813, 1032
HpyCH4V	TGCA	8 cut(s)	845, 860, 1130, 1199, 1222, 1229, 1544, 1715
HpyF10VI	GCNNNNNNNGC	4 cut(s)	349, 353, 947, 1712
HpyF3I	CTNAG	6 cut(s)	98, 113, 216, 255, 261, 636
HpySE526I	ACGT	2 cut(s)	813, 1032
Hsp92II	CATG	9 cut(s)	385, 472, 524, 631, 770, 1087, 1094, 1414, 1674
HspAI	GCGC	2 cut(s)	117, 119
KpnI	GGTACC	1 cut(s)	328
Ksp22I	TGATCA	2 cut(s)	1146, 1258
KspAI	GTTAAC	1 cut(s)	676
Kzo9I	GATC	4 cut(s)	658, 931, 1146, 1258
LguI	GCTCTTC	1 cut(s)	128
LmnI	GCTCC	3 cut(s)	203, 574, 1414
Lsp1109I	GCAGC	2 cut(s)	279, 1183
LweI	GCATC	2 cut(s)	696, 1208
MaeI	CTAG	2 cut(s)	516, 1584
MaeII	ACGT	2 cut(s)	813, 1032
MaeIII	GTNAC	8 cut(s)	153, 167, 292, 401, 464, 553, 650, 691
MalI	GATC	4 cut(s)	660, 933, 1148, 1260
MboI	GATC	4 cut(s)	658, 931, 1146, 1258
MhlI	GDGCHC	4 cut(s)	224, 354, 380, 1419
MlsI	TGGCCA	1 cut(s)	459
MluNI	TGGCCA	1 cut(s)	459
MlyI	GAGTC	7 cut(s)	54, 96, 253, 255, 285, 1247, 1314
MmeI	TCCRAC	2 cut(s)	210, 1431
Mox20I	TGGCCA	1 cut(s)	459
MroXI	GAANNNNTTC	3 cut(s)	420, 1185, 1397
MscI	TGGCCA	1 cut(s)	459
MseI	TTAA	6 cut(s)	447, 675, 1109, 1242, 1601, 1665
Msp20I	TGGCCA	1 cut(s)	459
MspA1I	CMGCKG	2 cut(s)	165, 1694
MspI	CCGG	1 cut(s)	368
MspR9I	CCNGG	3 cut(s)	1272, 1380, 1553
Mva1269I	GAATGC	1 cut(s)	1198
MvaI	CCWGG	3 cut(s)	1272, 1380, 1553
MvnI	CGCG	3 cut(s)	71, 119, 180
MwoI	GCNNNNNNNGC	4 cut(s)	349, 353, 947, 1712
NcoI	CCATGG	3 cut(s)	381, 1090, 1410
NdeI	CATATG	2 cut(s)	410, 1525
NdeII	GATC	4 cut(s)	658, 931, 1146, 1258
NheI	GCTAGC	1 cut(s)	515
NlaIII	CATG	9 cut(s)	385, 472, 524, 631, 770, 1087, 1094, 1414, 1674
NlaIV	GGNNCC	4 cut(s)	326, 379, 955, 1416
NmeAIII	GCCGAG	1 cut(s)	269
NmuCI	GTSAC	4 cut(s)	153, 292, 553, 691
NspI	RCATGY	3 cut(s)	472, 631, 1674
PagI	TCATGA	1 cut(s)	1083
PasI	CCCWGGG	1 cut(s)	1552
PauI	GCGCGC	1 cut(s)	117
PciI	ACATGT	2 cut(s)	468, 627
PciSI	GCTCTTC	1 cut(s)	128
PctI	GAATGC	1 cut(s)	1198
PdmI	GAANNNNTTC	3 cut(s)	420, 1185, 1397
PfeI	GAWTC	2 cut(s)	643, 1459
Pfl23II	CGTACG	1 cut(s)	181
PflMI	CCANNNNNTGG	1 cut(s)	1354
PfoI	TCCNGGA	1 cut(s)	1378
PkrI	GCNGC	3 cut(s)	269, 1198, 1656
PleI	GAGTC	7 cut(s)	54, 96, 253, 254, 284, 1247, 1313
PmaCI	CACGTG	1 cut(s)	1033
PmlI	CACGTG	1 cut(s)	1033
PpsI	GAGTC	7 cut(s)	54, 96, 253, 254, 284, 1247, 1313
Ppu21I	YACGTR	1 cut(s)	1033
PpuMI	RGGWCCY	2 cut(s)	903, 1571
PscI	ACATGT	2 cut(s)	468, 627
Psp124BI	GAGCTC	1 cut(s)	354
Psp5II	RGGWCCY	2 cut(s)	903, 1571
Psp6I	CCWGG	3 cut(s)	1270, 1378, 1551
PspCI	CACGTG	1 cut(s)	1033
PspGI	CCWGG	3 cut(s)	1270, 1378, 1551
PspLI	CGTACG	1 cut(s)	181
PspN4I	GGNNCC	4 cut(s)	326, 379, 955, 1416
PspPI	GGNCC	5 cut(s)	283, 365, 903, 1328, 1571
PspPPI	RGGWCCY	2 cut(s)	903, 1571
PstI	CTGCAG	1 cut(s)	1231
PteI	GCGCGC	1 cut(s)	117
RsaI	GTAC	4 cut(s)	29, 183, 326, 1060
RsaNI	GTAC	4 cut(s)	28, 182, 325, 1059
Rsr2I	CGGWCCG	1 cut(s)	365
RsrII	CGGWCCG	1 cut(s)	365
SacI	GAGCTC	1 cut(s)	354
SalI	GTCGAC	1 cut(s)	248
SapI	GCTCTTC	1 cut(s)	128
SaqAI	TTAA	6 cut(s)	447, 675, 1109, 1242, 1601, 1665
SatI	GCNGC	3 cut(s)	268, 1197, 1655
Sau3AI	GATC	4 cut(s)	658, 931, 1146, 1258
Sau96I	GGNCC	5 cut(s)	283, 365, 903, 1328, 1571
SchI	GAGTC	7 cut(s)	54, 96, 253, 255, 285, 1247, 1314
ScrFI	CCNGG	3 cut(s)	1272, 1380, 1553
SduI	GDGCHC	4 cut(s)	224, 354, 380, 1419
SfaNI	GCATC	2 cut(s)	696, 1208
SfcI	CTRYAG	1 cut(s)	1227
SinI	GGWCC	4 cut(s)	283, 365, 903, 1571
SsiI	CCGC	9 cut(s)	69, 132, 142, 163, 178, 201, 709, 1655, 1694
SspI	AATATT	1 cut(s)	1507
SspMI	CTAG	2 cut(s)	516, 1584
SstI	GAGCTC	1 cut(s)	354
StyD4I	CCNGG	3 cut(s)	1270, 1378, 1551
StyI	CCWWGG	4 cut(s)	381, 1090, 1410, 1574
TaaI	ACNGT	9 cut(s)	226, 365, 401, 464, 483, 492, 656, 1002, 1354
TaiI	ACGT	2 cut(s)	816, 1035
TaqI	TCGA	6 cut(s)	249, 330, 598, 865, 1248, 1343
TaqII	GACCGA	2 cut(s)	291, 300
TatI	WGTACW	1 cut(s)	27
TauI	GCSGC	1 cut(s)	1657
TfiI	GAWTC	2 cut(s)	643, 1459
Tru1I	TTAA	6 cut(s)	447, 675, 1109, 1242, 1601, 1665
Tru9I	TTAA	6 cut(s)	447, 675, 1109, 1242, 1601, 1665
TscAI	CASTG	1 cut(s)	644
TseFI	GTSAC	4 cut(s)	153, 292, 553, 691
TseI	GCWGC	2 cut(s)	267, 1196
Tsp45I	GTSAC	4 cut(s)	153, 292, 553, 691
TspDTI	ATGAA	5 cut(s)	635, 1026, 1313, 1545, 1605
TspGWI	ACGGA	1 cut(s)	380
TspRI	CASTG	1 cut(s)	644
Van91I	CCANNNNNTGG	1 cut(s)	1354
VpaK11BI	GGWCC	4 cut(s)	283, 365, 903, 1571
XapI	RAATTY	5 cut(s)	443, 737, 824, 976, 1280
XceI	RCATGY	3 cut(s)	472, 631, 1674
XmiI	GTMKAC	2 cut(s)	249, 818
XmnI	GAANNNNTTC	3 cut(s)	420, 1185, 1397
XspI	CTAG	2 cut(s)	516, 1584

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.