pycom04g10600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014506
Physical Location & Seq
Forward (+)
13314020 .. 13316046
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGGGGCCACCGGTCCATTTTTGTACCCGAGAATACGAAATAGCGCGAAGCGCGAACTGGTCACGGTCAAACCCTGGTTTTCGTTTCTCTCTTCCCTCGTCAACTTCGAGCTCACGACAACATAAGATTCGAACGAACACGCCGTTAAACCACCGCCACAACCTCCACCACCGCACTTTTAATTTGACGAAATTGCTCCTCGCGGCTGTGTTCTCAGGGAGGAAAGGAGAGAGGAGCTGCGTCGCGCTCGCACCCCCCCCCCCCCCCCCCCGCCCCCGCGGGGCGTTTCTTCCGATCATCGTTTCGTCCAAAATTTTGAATTTTAAAAATAAATTAGTCGCGATTAAACTCGCCATGTCGATTAGGGAGCTGCGGAGCTCTACGGTTTCGGTCAATTTGTTCACCTTCTGCGTTTTCCTCAGCTGCTTTCCAGGATTTATATTATCGGCGATCGGTACGCTGTATTCGATCGTGATATACAACACGCATGAATTGATAAAGGCTGTGAAACCGAAGGTGTATTTCCAATGCATAGGGGAGAACAAGACGAATTTTCCAGACGTGAAGGACAAGAACGTCAGGTATAATTTCAACGGCGAAGAGTCTTGGCAGCCTCTGACAGAGTTTTCGGGTAAGAAGTGCAAGCGATGTGGATTGTATGAGGCGGATACCTTTAAACCAGATGACAAATTCGATGAGTGGGAGTTGTGTCCTTCTGACTTCAATGCTTCTGATGGGAAATATGTGCGGTTCAAGAACAAGGAATTCAACATTACGTTTTGGTGCCCAAAGTGTGTACCTGATATACCTGCTTCAAGTTTCACCACTGCGCCGAACAAAGAAGAAAAGGAGAAGGGAATGCATGTTGTTCTAATACTTTTAATCGTTGTAGCCGTTTTATCAGTGTGTATTGTCGTTGGCTTTGCTGCGTACAAGTACTGGCAAAAGAAGAAGAGAGAGCAAGCTTTGATCGATCAGGCTCGGTTCCTAAAACTGTTTGAAGACGGGGATGATATTGAAGATGAAATGGGGCTTGACCATATAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

350

Amino Acids

39.78

Weight (kDa)

9.25

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7953 PF25829 150 - 263 1.1e-52 Domain of unknown function (DUF7953)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53490
fragaria_vesca FvH4_6g14800
malus_domestica MD04G1110000.v1.1 MD12G1131500.v1.1
prunus_persica Prupe.6G245800_v2.0.a1
pyrus_communis pycom04g10600 pycom12g12720
rosa_chinensis RchiOBHm_Chr3g0468021
rosa_laevigata RLG00000024458
rosa_multiflora Rmu_sc0006706.1_g000009
rosa_roxburghii Rroxscaffold_6G00413170
rosa_rugosa Rorug03G0091500
rosa_samantha Rh3AG140200 Rh3BG161400 Rh3CG161500 Rh3DG162000
rosa_wichuraiana Rw3G013240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 575
Acc36I ACCTGC 1 cut(s) 817
AccB1I GGYRCC 1 cut(s) 783
AccII CGCG 6 cut(s) 46, 53, 203, 245, 279, 341
AciI CCGC 9 cut(s) 154, 172, 203, 271, 277, 279, 373, 665, 748
AcsI RAATTY 5 cut(s) 312, 319, 550, 689, 764
AfaI GTAC 5 cut(s) 25, 457, 798, 932, 938
AfiI CCNNNNNNNGG 1 cut(s) 280
AgeI ACCGGT 1 cut(s) 10
AgsI TTSAA 8 cut(s) 319, 592, 724, 754, 769, 816, 1001, 1019
AjiI CACGTC 1 cut(s) 562
AjnI CCWGG 2 cut(s) 73, 430
AluBI AGCT 6 cut(s) 111, 237, 370, 378, 423, 965
AluI AGCT 6 cut(s) 111, 237, 370, 378, 423, 965
Alw21I GWGCWC 2 cut(s) 113, 380
AlwNI CAGNNNCTG 1 cut(s) 616
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 5 cut(s) 237, 370, 423, 610, 926
ApoI RAATTY 5 cut(s) 312, 319, 550, 689, 764
ArsI GACNNNNNNTTYG 2 cut(s) 610, 642
AsiGI ACCGGT 1 cut(s) 10
AspLEI GCGC 4 cut(s) 46, 53, 247, 832
AspS9I GGNCC 2 cut(s) 5, 13
AsuHPI GGTGA 2 cut(s) 394, 814
AsuII TTCGAA 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 788
BanI GGYRCC 1 cut(s) 783
BanII GRGCYC 2 cut(s) 113, 380
BbsI GAAGAC 1 cut(s) 1008
Bbv12I GWGCWC 2 cut(s) 113, 380
BbvCI CCTCAGC 1 cut(s) 419
BbvI GCAGC 5 cut(s) 224, 357, 410, 622, 913
BccI CCATC 1 cut(s) 728
BceAI ACGGC 3 cut(s) 127, 610, 878
BciT130I CCWGG 2 cut(s) 75, 432
BciVI GTATCC 1 cut(s) 661
BfuAI ACCTGC 1 cut(s) 817
BfuI GTATCC 1 cut(s) 661
BisI GCNGC 6 cut(s) 204, 238, 371, 424, 611, 927
BlsI GCNGC 6 cut(s) 205, 239, 372, 425, 612, 928
BmcAI AGTACT 1 cut(s) 938
Bme1390I CCNGG 2 cut(s) 75, 432
Bme18I GGWCC 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 27
BmgBI CACGTC 1 cut(s) 562
BmgT120I GGNCC 2 cut(s) 5, 13
BmiI GGNNCC 3 cut(s) 6, 785, 986
BmrFI CCNGG 2 cut(s) 75, 432
BpiI GAAGAC 1 cut(s) 1008
Bpu10I CCTNAGC 1 cut(s) 419
Bpu14I TTCGAA 1 cut(s) 130
Bsa29I ATCGAT 1 cut(s) 972
BsaJI CCNNGG 2 cut(s) 73, 277
BsaWI WCCGGW 1 cut(s) 10
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 2 cut(s) 62, 944
BseBI CCWGG 2 cut(s) 75, 432
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 2 cut(s) 73, 277
BseGI GGATG 1 cut(s) 1015
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMII CTCAG 2 cut(s) 228, 433
BseNI ACTGG 2 cut(s) 62, 944
BseRI GAGGAG 2 cut(s) 188, 247
BseSI GKGCMC 1 cut(s) 788
BseXI GCAGC 5 cut(s) 224, 357, 410, 622, 913
Bsh1236I CGCG 6 cut(s) 46, 53, 203, 245, 279, 341
Bsh1285I CGRYCG 2 cut(s) 453, 471
BshFI GGCC 1 cut(s) 7
BshNI GGYRCC 1 cut(s) 783
BshTI ACCGGT 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 972
BsiEI CGRYCG 2 cut(s) 453, 471
BsiHKAI GWGCWC 2 cut(s) 113, 380
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 1 cut(s) 11
BslI CCNNNNNNNGG 1 cut(s) 280
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 1 cut(s) 7
BsoBI CYCGRG 1 cut(s) 27
Bsp119I TTCGAA 1 cut(s) 130
Bsp1286I GDGCHC 3 cut(s) 113, 380, 788
Bsp143I GATC 5 cut(s) 294, 450, 468, 969, 973
Bsp68I TCGCGA 1 cut(s) 341
BspACI CCGC 9 cut(s) 154, 172, 203, 271, 277, 279, 373, 665, 748
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 2 cut(s) 227, 432
BspDI ATCGAT 1 cut(s) 972
BspFNI CGCG 6 cut(s) 46, 53, 203, 245, 279, 341
BspLI GGNNCC 3 cut(s) 6, 785, 986
BspMI ACCTGC 1 cut(s) 817
BspT104I TTCGAA 1 cut(s) 130
BspT107I GGYRCC 1 cut(s) 783
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 2 cut(s) 62, 944
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 73, 277
BssMI GATC 5 cut(s) 294, 450, 468, 969, 973
Bst2UI CCWGG 2 cut(s) 75, 432
Bst4CI ACNGT 3 cut(s) 66, 385, 996
Bst6I CTCTTC 3 cut(s) 96, 594, 947
BstBI TTCGAA 1 cut(s) 130
BstC8I GCNNGC 3 cut(s) 249, 644, 963
BstDEI CTNAG 2 cut(s) 214, 419
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 1015
BstFNI CGCG 6 cut(s) 46, 53, 203, 245, 279, 341
BstHHI GCGC 4 cut(s) 46, 53, 247, 832
BstKTI GATC 5 cut(s) 297, 453, 471, 972, 976
BstMBI GATC 5 cut(s) 294, 450, 468, 969, 973
BstMCI CGRYCG 2 cut(s) 453, 471
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 2 cut(s) 75, 432
BstNSI RCATGY 1 cut(s) 866
BstSCI CCNGG 2 cut(s) 73, 430
BstSLI GKGCMC 1 cut(s) 788
BstUI CGCG 6 cut(s) 46, 53, 203, 245, 279, 341
BstV1I GCAGC 5 cut(s) 224, 357, 410, 622, 913
BstV2I GAAGAC 1 cut(s) 1008
Bsu15I ATCGAT 1 cut(s) 972
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 1 cut(s) 7
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 277
BtgZI GCGATG 1 cut(s) 661
BtrI CACGTC 1 cut(s) 562
BtsCI GGATG 1 cut(s) 1015
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 2 cut(s) 825, 909
BtuMI TCGCGA 1 cut(s) 341
BveI ACCTGC 1 cut(s) 817
Cac8I GCNNGC 3 cut(s) 249, 644, 963
CaiI CAGNNNCTG 1 cut(s) 616
CfoI GCGC 4 cut(s) 46, 53, 247, 832
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 5, 13
Cfr42I CCGCGG 1 cut(s) 280
ClaI ATCGAT 1 cut(s) 972
CseI GACGC 1 cut(s) 229
Csp6I GTAC 5 cut(s) 24, 456, 797, 931, 937
CspAI ACCGGT 1 cut(s) 10
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviAII CATG 3 cut(s) 355, 488, 863
CviQI GTAC 5 cut(s) 24, 456, 797, 931, 937
DdeI CTNAG 2 cut(s) 214, 419
DpnI GATC 5 cut(s) 296, 452, 470, 971, 975
DpnII GATC 5 cut(s) 294, 450, 468, 969, 973
DraI TTTAAA 2 cut(s) 325, 676
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
Eam1104I CTCTTC 3 cut(s) 96, 594, 947
EarI CTCTTC 3 cut(s) 96, 594, 947
EciI GGCGGA 1 cut(s) 680
Ecl136II GAGCTC 2 cut(s) 111, 378
Eco24I GRGCYC 2 cut(s) 113, 380
Eco47I GGWCC 1 cut(s) 13
Eco53kI GAGCTC 2 cut(s) 111, 378
Eco88I CYCGRG 1 cut(s) 27
EcoICRI GAGCTC 2 cut(s) 111, 378
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 73, 430
EcoT22I ATGCAT 2 cut(s) 533, 864
EcoT38I GRGCYC 2 cut(s) 113, 380
FaeI CATG 3 cut(s) 358, 491, 866
FatI CATG 3 cut(s) 354, 487, 862
FauI CCCGC 3 cut(s) 272, 278, 284
Fnu4HI GCNGC 6 cut(s) 204, 238, 371, 424, 611, 927
FokI GGATG 1 cut(s) 1022
FriOI GRGCYC 2 cut(s) 113, 380
Fsp4HI GCNGC 6 cut(s) 204, 238, 371, 424, 611, 927
GlaI GCGC 4 cut(s) 45, 52, 246, 831
GluI GCNGC 6 cut(s) 204, 238, 371, 424, 611, 927
HaeIII GGCC 1 cut(s) 7
HapII CCGG 1 cut(s) 11
HgaI GACGC 1 cut(s) 229
HhaI GCGC 4 cut(s) 46, 53, 247, 832
Hin1II CATG 3 cut(s) 358, 491, 866
Hin6I GCGC 4 cut(s) 44, 51, 245, 830
HinP1I GCGC 4 cut(s) 44, 51, 245, 830
HincII GTYRAC 1 cut(s) 102
HindII GTYRAC 1 cut(s) 102
HindIII AAGCTT 1 cut(s) 963
HinfI GANTC 2 cut(s) 127, 602
HpaII CCGG 1 cut(s) 11
HphI GGTGA 2 cut(s) 394, 814
Hpy166II GTNNAC 3 cut(s) 102, 402, 797
Hpy188I TCNGA 5 cut(s) 294, 618, 718, 733, 1049
Hpy188III TCNNGA 5 cut(s) 114, 340, 472, 557, 754
Hpy8I GTNNAC 3 cut(s) 102, 402, 797
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 5 cut(s) 415, 508, 559, 723, 847
HpyCH4III ACNGT 3 cut(s) 66, 385, 996
HpyCH4IV ACGT 3 cut(s) 561, 576, 776
HpyCH4V TGCA 3 cut(s) 531, 642, 862
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 2 cut(s) 214, 419
HpySE526I ACGT 3 cut(s) 561, 576, 776
Hsp92II CATG 3 cut(s) 358, 491, 866
HspAI GCGC 4 cut(s) 44, 51, 245, 830
KspI CCGCGG 1 cut(s) 280
Kzo9I GATC 5 cut(s) 294, 450, 468, 969, 973
LmnI GCTCC 4 cut(s) 201, 234, 367, 375
Lsp1109I GCAGC 5 cut(s) 224, 357, 410, 622, 913
MaeII ACGT 3 cut(s) 561, 576, 776
MaeIII GTNAC 1 cut(s) 60
MalI GATC 5 cut(s) 296, 452, 470, 971, 975
MboI GATC 5 cut(s) 294, 450, 468, 969, 973
MboII GAAGA 8 cut(s) 83, 281, 611, 854, 961, 964, 1013, 1031
MhlI GDGCHC 3 cut(s) 113, 380, 788
MlyI GAGTC 1 cut(s) 611
MnlI CCTC 8 cut(s) 106, 173, 209, 213, 225, 428, 624, 655
Mph1103I ATGCAT 2 cut(s) 533, 864
MseI TTAA 6 cut(s) 146, 180, 324, 345, 675, 881
MspA1I CMGCKG 2 cut(s) 279, 423
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 2 cut(s) 75, 432
Mva1269I GAATGC 1 cut(s) 864
MvaI CCWGG 2 cut(s) 75, 432
MvnI CGCG 6 cut(s) 46, 53, 203, 245, 279, 341
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 5 cut(s) 294, 450, 468, 969, 973
NlaIII CATG 3 cut(s) 358, 491, 866
NlaIV GGNNCC 3 cut(s) 6, 785, 986
NmuCI GTSAC 1 cut(s) 60
NruI TCGCGA 1 cut(s) 341
NsiI ATGCAT 2 cut(s) 533, 864
NspI RCATGY 1 cut(s) 866
NspV TTCGAA 1 cut(s) 130
PcsI WCGNNNNNNNCGW 4 cut(s) 104, 140, 464, 891
PctI GAATGC 1 cut(s) 864
PfeI GAWTC 1 cut(s) 127
PfoI TCCNGGA 1 cut(s) 430
PinAI ACCGGT 1 cut(s) 10
PkrI GCNGC 6 cut(s) 205, 239, 372, 425, 612, 928
Ple19I CGATCG 2 cut(s) 453, 471
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
Psp124BI GAGCTC 2 cut(s) 113, 380
Psp6I CCWGG 2 cut(s) 73, 430
PspGI CCWGG 2 cut(s) 73, 430
PspN4I GGNNCC 3 cut(s) 6, 785, 986
PspPI GGNCC 2 cut(s) 5, 13
PstNI CAGNNNCTG 1 cut(s) 616
PvuI CGATCG 2 cut(s) 453, 471
PvuII CAGCTG 1 cut(s) 423
RruI TCGCGA 1 cut(s) 341
RsaI GTAC 5 cut(s) 25, 457, 798, 932, 938
RsaNI GTAC 5 cut(s) 24, 456, 797, 931, 937
SacI GAGCTC 2 cut(s) 113, 380
SacII CCGCGG 1 cut(s) 280
SaqAI TTAA 6 cut(s) 146, 180, 324, 345, 675, 881
SatI GCNGC 6 cut(s) 204, 238, 371, 424, 611, 927
Sau3AI GATC 5 cut(s) 294, 450, 468, 969, 973
Sau96I GGNCC 2 cut(s) 5, 13
ScaI AGTACT 1 cut(s) 938
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 2 cut(s) 75, 432
SduI GDGCHC 3 cut(s) 113, 380, 788
Sfr303I CCGCGG 1 cut(s) 280
SfuI TTCGAA 1 cut(s) 130
SgrBI CCGCGG 1 cut(s) 280
SinI GGWCC 1 cut(s) 13
SsiI CCGC 9 cut(s) 154, 172, 203, 271, 277, 279, 373, 665, 748
SstI GAGCTC 2 cut(s) 113, 380
StyD4I CCNGG 2 cut(s) 73, 430
TaaI ACNGT 3 cut(s) 66, 385, 996
TaiI ACGT 3 cut(s) 564, 579, 779
TaqI TCGA 6 cut(s) 107, 130, 359, 467, 693, 972
TaqII GACCGA 1 cut(s) 379
TatI WGTACW 1 cut(s) 936
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 6 cut(s) 146, 180, 324, 345, 675, 881
Tru9I TTAA 6 cut(s) 146, 180, 324, 345, 675, 881
TscAI CASTG 2 cut(s) 832, 909
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 5 cut(s) 237, 370, 423, 610, 926
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 2 cut(s) 504, 1038
TspRI CASTG 2 cut(s) 832, 909
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 5 cut(s) 312, 319, 550, 689, 764
XceI RCATGY 1 cut(s) 866
ZrmI AGTACT 1 cut(s) 938
Zsp2I ATGCAT 2 cut(s) 533, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.