pycom04g10650

GPR107-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012152
Physical Location & Seq
Reverse (-)
13416702 .. 13417577
876 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g10650.1

Sequence Viewer

Length: 876 bp
ATGATCGTACGGACAGAAGTCTACAATCTTGACCCGGATGGTTCCCACGACTACCTTTCGGCCGGGTTGACCAACCTGCCGTCGCTGTTTTCGGTCTTTTTTCTCGCGTATTTGGTGTTTGTGGGCTTCTGGGTCTACCTCTGCTACACCAACAAGAGATCCGTGCACCGGATCCATCTTCTGATGGGCGGATTACTTTTGATGAAGGCGTTGAATCTGATTAGCGCGGCGGAGGACAAGCACTACGTGAAGGTCACCGGCACCGCGCACGGCTGGGATGTGTTGTTCTACATCTTCCAGTTCCTCCGTGTCATTTTGTTGTTTACCGTGATCGTTTTGATCGGGACCGGGTGGTCGTTCTTGAAGCCTTTTTTGCAGGAGAAGGAGAAGAAGGTGTTGATGATTGTGATCCCGCTACAGGTCTTGGCCAACGTAGCGTCGATTGTGATCGGCGAGACGGGACCATTTATCAAGGACTGGGTCACCTGGAACCAGATCTTCTTGTTGGTTGATATCATTTGCTGCTGCGCTATCATCTTCCCCATTGTTTGGTCCATCAGGTCTCTGAGAGAAACCTCAAAAACCGACGGAAAAGCCGCCAGGAACTTGGCAAAGCTGACCCTTTTCAGGCAATTTTACGTTGTCGTTATTGGGTACTTGTACTTCACGCGAATCGTTGTTTTTGCGCTCAAGACCATTGCCGCGTATAAGTACCAGTGGGTGAGCAATGCGGCTGAGGAGACTGTGAGCCTTGCGTTTTACATGGTGATGTTCTATATGTTCAGGCCGGTGGAGAGGAATGAGTACTTTATTCTCGATGAGGAGGAAGAAGAGGCGGCAGAGGTGGCTCTTAGGGACGAGGAGTTCGAGCTTTAA

Protein Analysis

292

Amino Acids

33.66

Weight (kDa)

6.0

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 27 - 267 2.1e-30 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012152)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09570 AT5G18520
fragaria_vesca FvH4_6g14730
malus_domestica MD04G1116700.v1.1 MD12G1132400.v1.1
prunus_persica Prupe.6G246500_v2.0.a1
pyrus_communis pycom04g10650
rosa_chinensis RchiOBHm_Chr3g0467951
rosa_laevigata RLG00000024464
rosa_multiflora Rmu_sc0001793.1_g000057 Rmu_sc0006017.1_g000004 Rmu_sc0034877.1_g000003
rosa_roxburghii Rroxscaffold_6G00413260
rosa_rugosa Rorug03G0090600
rosa_samantha Rh3AG139300 Rh3BG160800 Rh3CG160700 Rh3DG161300
rosa_wichuraiana Rw3G013180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 352
Acc36I ACCTGC 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 549
AccI GTMKAC 2 cut(s) 21, 135
AccII CGCG 5 cut(s) 107, 227, 266, 670, 704
AciI CCGC 9 cut(s) 189, 227, 230, 264, 413, 597, 702, 731, 836
AclWI GGATC 4 cut(s) 153, 166, 179, 403
AcoI YGGCCR 2 cut(s) 60, 426
AdeI CACNNNGTG 1 cut(s) 247
AfaI GTAC 5 cut(s) 9, 656, 662, 713, 806
AfiI CCNNNNNNNGG 4 cut(s) 168, 418, 549, 627
AgsI TTSAA 2 cut(s) 214, 364
AjnI CCWGG 2 cut(s) 485, 599
AloI GAACNNNNNNTCC 3 cut(s) 269, 301, 848
AluBI AGCT 2 cut(s) 616, 871
AluI AGCT 2 cut(s) 616, 871
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 3 cut(s) 449, 567, 734
Alw44I GTGCAC 1 cut(s) 164
AlwI GGATC 4 cut(s) 153, 166, 179, 403
AoxI GGCC 3 cut(s) 60, 426, 785
ApaLI GTGCAC 1 cut(s) 164
ApeKI GCWGC 2 cut(s) 522, 525
ArsI GACNNNNNNTTYG 1 cut(s) 848
AspLEI GCGC 4 cut(s) 227, 268, 530, 688
AspS9I GGNCC 3 cut(s) 345, 461, 552
AsuC2I CCSGG 3 cut(s) 35, 64, 349
AsuHPI GGTGA 4 cut(s) 247, 475, 733, 778
AvaII GGWCC 3 cut(s) 345, 461, 552
BaeGI GKGCMC 1 cut(s) 168
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 1 cut(s) 171
BanI GGYRCC 1 cut(s) 260
BarI GAAGNNNNNNTAC 2 cut(s) 647, 679
Bbv12I GWGCWC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 2 cut(s) 509, 512
BccI CCATC 4 cut(s) 32, 178, 183, 563
BceAI ACGGC 2 cut(s) 64, 286
BciT130I CCWGG 2 cut(s) 487, 601
BcnI CCSGG 3 cut(s) 35, 64, 349
BcoDI GTCTC 3 cut(s) 449, 567, 734
BfmI CTRYAG 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 84
BglII AGATCT 1 cut(s) 495
BisI GCNGC 7 cut(s) 228, 523, 526, 597, 702, 732, 837
BlsI GCNGC 7 cut(s) 229, 524, 527, 598, 703, 733, 838
BmcAI AGTACT 1 cut(s) 806
Bme1390I CCNGG 5 cut(s) 35, 64, 349, 487, 601
Bme18I GGWCC 3 cut(s) 345, 461, 552
BmgT120I GGNCC 3 cut(s) 345, 461, 552
BmiI GGNNCC 6 cut(s) 43, 173, 262, 346, 462, 491
BmrFI CCNGG 5 cut(s) 35, 64, 349, 487, 601
BmrI ACTGGG 1 cut(s) 487
BmuI ACTGGG 1 cut(s) 487
BoxI GACNNNNGTC 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 3 cut(s) 35, 64, 349
BsaAI YACGTR 1 cut(s) 247
BsaBI GATNNNNATC 2 cut(s) 407, 446
BsaI GGTCTC 1 cut(s) 567
BsaWI WCCGGW 1 cut(s) 168
Bsc4I CCNNNNNNNGG 4 cut(s) 168, 418, 549, 627
Bse118I RCCGGY 2 cut(s) 257, 787
Bse1I ACTGG 3 cut(s) 298, 482, 715
Bse3DI GCAATG 2 cut(s) 696, 733
Bse8I GATNNNNATC 2 cut(s) 407, 446
BseBI CCWGG 2 cut(s) 487, 601
BseGI GGATG 2 cut(s) 43, 283
BseJI GATNNNNATC 2 cut(s) 407, 446
BseLI CCNNNNNNNGG 4 cut(s) 168, 418, 549, 627
BseMI GCAATG 2 cut(s) 696, 733
BseMII CTCAG 2 cut(s) 557, 726
BseNI ACTGG 3 cut(s) 298, 482, 715
BseRI GAGGAG 3 cut(s) 752, 836, 875
BseSI GKGCMC 1 cut(s) 168
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 2 cut(s) 509, 512
BseYI CCCAGC 1 cut(s) 273
Bsh1236I CGCG 5 cut(s) 107, 227, 266, 670, 704
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 3 cut(s) 62, 428, 787
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 6 cut(s) 35, 63, 169, 258, 348, 788
BsiWI CGTACG 1 cut(s) 7
BslFI GGGAC 3 cut(s) 358, 474, 869
BslI CCNNNNNNNGG 4 cut(s) 168, 418, 549, 627
BsmAI GTCTC 3 cut(s) 449, 567, 734
BsmBI CGTCTC 1 cut(s) 449
BsmFI GGGAC 3 cut(s) 358, 474, 869
BsnI GGCC 3 cut(s) 62, 428, 787
Bso31I GGTCTC 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 8 cut(s) 3, 158, 171, 330, 339, 408, 447, 495
BspACI CCGC 9 cut(s) 189, 227, 230, 264, 413, 597, 702, 731, 836
BspANI GGCC 3 cut(s) 62, 428, 787
BspCNI CTCAG 2 cut(s) 558, 727
BspFNI CGCG 5 cut(s) 107, 227, 266, 670, 704
BspLI GGNNCC 6 cut(s) 43, 173, 262, 346, 462, 491
BspMI ACCTGC 1 cut(s) 84
BspPI GGATC 4 cut(s) 153, 166, 179, 403
BspT107I GGYRCC 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 567
BsrDI GCAATG 2 cut(s) 696, 733
BsrFI RCCGGY 2 cut(s) 257, 787
BsrI ACTGG 3 cut(s) 298, 482, 715
BssAI RCCGGY 2 cut(s) 257, 787
BssMI GATC 8 cut(s) 3, 158, 171, 330, 339, 408, 447, 495
Bst2UI CCWGG 2 cut(s) 487, 601
Bst4CI ACNGT 2 cut(s) 328, 745
Bst6I CTCTTC 1 cut(s) 825
BstBAI YACGTR 1 cut(s) 247
BstDEI CTNAG 3 cut(s) 566, 735, 851
BstEII GGTNACC 2 cut(s) 253, 481
BstF5I GGATG 2 cut(s) 43, 283
BstFNI CGCG 5 cut(s) 107, 227, 266, 670, 704
BstHHI GCGC 4 cut(s) 227, 268, 530, 688
BstKTI GATC 8 cut(s) 6, 161, 174, 333, 342, 411, 450, 498
BstMAI GTCTC 3 cut(s) 449, 567, 734
BstMBI GATC 8 cut(s) 3, 158, 171, 330, 339, 408, 447, 495
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 373, 434, 845
BstNI CCWGG 2 cut(s) 487, 601
BstPAI GACNNNNGTC 1 cut(s) 17
BstPI GGTNACC 2 cut(s) 253, 481
BstSCI CCNGG 5 cut(s) 33, 62, 347, 485, 599
BstSFI CTRYAG 1 cut(s) 416
BstSLI GKGCMC 1 cut(s) 168
BstUI CGCG 5 cut(s) 107, 227, 266, 670, 704
BstV1I GCAGC 2 cut(s) 509, 512
BstX2I RGATCY 3 cut(s) 158, 171, 495
BstXI CCANNNNNNTGG 1 cut(s) 607
BstYI RGATCY 3 cut(s) 158, 171, 495
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 3 cut(s) 62, 428, 787
BtsCI GGATG 2 cut(s) 43, 283
BtsIMutI CAGTG 1 cut(s) 722
BveI ACCTGC 1 cut(s) 84
CfoI GCGC 4 cut(s) 227, 268, 530, 688
Cfr10I RCCGGY 2 cut(s) 257, 787
Cfr13I GGNCC 3 cut(s) 345, 461, 552
CseI GACGC 1 cut(s) 426
Csp6I GTAC 5 cut(s) 8, 655, 661, 712, 805
CviAII CATG 1 cut(s) 763
CviQI GTAC 5 cut(s) 8, 655, 661, 712, 805
DdeI CTNAG 3 cut(s) 566, 735, 851
DpnI GATC 8 cut(s) 5, 160, 173, 332, 341, 410, 449, 497
DpnII GATC 8 cut(s) 3, 158, 171, 330, 339, 408, 447, 495
DraIII CACNNNGTG 1 cut(s) 247
DrdI GACNNNNNNGTC 1 cut(s) 352
DseDI GACNNNNNNGTC 1 cut(s) 352
EaeI YGGCCR 2 cut(s) 60, 426
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 825
EarI CTCTTC 1 cut(s) 825
EciI GGCGGA 2 cut(s) 204, 245
EclXI CGGCCG 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 567
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 3 cut(s) 345, 461, 552
Eco52I CGGCCG 1 cut(s) 60
Eco91I GGTNACC 2 cut(s) 253, 481
EcoO65I GGTNACC 2 cut(s) 253, 481
EcoRII CCWGG 2 cut(s) 485, 599
EcoRV GATATC 1 cut(s) 514
Esp3I CGTCTC 1 cut(s) 449
FaeI CATG 1 cut(s) 766
FaiI YATR 4 cut(s) 708, 764, 777, 779
FaqI GGGAC 3 cut(s) 358, 474, 869
FatI CATG 1 cut(s) 762
FauI CCCGC 1 cut(s) 420
FblI GTMKAC 2 cut(s) 21, 135
Fnu4HI GCNGC 7 cut(s) 228, 523, 526, 597, 702, 732, 837
FokI GGATG 2 cut(s) 50, 290
Fsp4HI GCNGC 7 cut(s) 228, 523, 526, 597, 702, 732, 837
GlaI GCGC 4 cut(s) 226, 267, 529, 687
GluI GCNGC 7 cut(s) 228, 523, 526, 597, 702, 732, 837
GsaI CCCAGC 1 cut(s) 277
HaeIII GGCC 3 cut(s) 62, 428, 787
HapII CCGG 6 cut(s) 35, 63, 169, 258, 348, 788
HgaI GACGC 1 cut(s) 426
HhaI GCGC 4 cut(s) 227, 268, 530, 688
Hin1II CATG 1 cut(s) 766
Hin6I GCGC 4 cut(s) 225, 266, 528, 686
HinP1I GCGC 4 cut(s) 225, 266, 528, 686
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 2 cut(s) 214, 672
HpaII CCGG 6 cut(s) 35, 63, 169, 258, 348, 788
HphI GGTGA 4 cut(s) 247, 475, 733, 778
Hpy166II GTNNAC 5 cut(s) 22, 69, 136, 166, 324
Hpy188I TCNGA 3 cut(s) 183, 219, 567
Hpy188III TCNNGA 5 cut(s) 29, 343, 361, 691, 815
Hpy8I GTNNAC 5 cut(s) 22, 69, 136, 166, 324
Hpy99I CGWCG 3 cut(s) 85, 442, 590
HpyAV CCTTC 4 cut(s) 199, 244, 376, 385
HpyCH4III ACNGT 2 cut(s) 328, 745
HpyCH4IV ACGT 3 cut(s) 246, 432, 639
HpyCH4V TGCA 2 cut(s) 166, 376
HpyF10VI GCNNNNNNNGC 3 cut(s) 373, 434, 845
HpyF3I CTNAG 3 cut(s) 566, 735, 851
HpySE526I ACGT 3 cut(s) 246, 432, 639
Hsp92II CATG 1 cut(s) 766
HspAI GCGC 4 cut(s) 225, 266, 528, 686
Kzo9I GATC 8 cut(s) 3, 158, 171, 330, 339, 408, 447, 495
Lsp1109I GCAGC 2 cut(s) 509, 512
MaeII ACGT 3 cut(s) 246, 432, 639
MaeIII GTNAC 2 cut(s) 253, 481
MalI GATC 8 cut(s) 5, 160, 173, 332, 341, 410, 449, 497
MboI GATC 8 cut(s) 3, 158, 171, 330, 339, 408, 447, 495
MboII GAAGA 7 cut(s) 170, 286, 400, 490, 529, 839, 842
MflI RGATCY 3 cut(s) 158, 171, 495
MhlI GDGCHC 1 cut(s) 168
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 1 cut(s) 632
MluNI TGGCCA 1 cut(s) 428
Mox20I TGGCCA 1 cut(s) 428
MscI TGGCCA 1 cut(s) 428
MseI TTAA 1 cut(s) 874
MslI CAYNNNNRTG 1 cut(s) 767
Msp20I TGGCCA 1 cut(s) 428
MspI CCGG 6 cut(s) 35, 63, 169, 258, 348, 788
MspR9I CCNGG 5 cut(s) 35, 64, 349, 487, 601
MvaI CCWGG 2 cut(s) 487, 601
MvnI CGCG 5 cut(s) 107, 227, 266, 670, 704
MwoI GCNNNNNNNGC 3 cut(s) 373, 434, 845
NciI CCSGG 3 cut(s) 35, 64, 349
NdeII GATC 8 cut(s) 3, 158, 171, 330, 339, 408, 447, 495
NlaIII CATG 1 cut(s) 766
NlaIV GGNNCC 6 cut(s) 43, 173, 262, 346, 462, 491
NmuCI GTSAC 2 cut(s) 253, 481
PcsI WCGNNNNNNNCGW 1 cut(s) 864
PfeI GAWTC 2 cut(s) 214, 672
Pfl23II CGTACG 1 cut(s) 7
PflFI GACNNNGTC 1 cut(s) 479
PflMI CCANNNNNTGG 1 cut(s) 549
PkrI GCNGC 7 cut(s) 229, 524, 527, 598, 703, 733, 838
Ppu21I YACGTR 1 cut(s) 247
PshAI GACNNNNGTC 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 485, 599
PspEI GGTNACC 2 cut(s) 253, 481
PspFI CCCAGC 1 cut(s) 273
PspGI CCWGG 2 cut(s) 485, 599
PspLI CGTACG 1 cut(s) 7
PspN4I GGNNCC 6 cut(s) 43, 173, 262, 346, 462, 491
PspPI GGNCC 3 cut(s) 345, 461, 552
PsuI RGATCY 3 cut(s) 158, 171, 495
PsyI GACNNNGTC 1 cut(s) 479
RsaI GTAC 5 cut(s) 9, 656, 662, 713, 806
RsaNI GTAC 5 cut(s) 8, 655, 661, 712, 805
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 1 cut(s) 874
SatI GCNGC 7 cut(s) 228, 523, 526, 597, 702, 732, 837
Sau3AI GATC 8 cut(s) 3, 158, 171, 330, 339, 408, 447, 495
Sau96I GGNCC 3 cut(s) 345, 461, 552
ScaI AGTACT 1 cut(s) 806
ScrFI CCNGG 5 cut(s) 35, 64, 349, 487, 601
SduI GDGCHC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 416
SinI GGWCC 3 cut(s) 345, 461, 552
SmiMI CAYNNNNRTG 1 cut(s) 767
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 1 cut(s) 632
SsiI CCGC 9 cut(s) 189, 227, 230, 264, 413, 597, 702, 731, 836
StyD4I CCNGG 5 cut(s) 33, 62, 347, 485, 599
TaaI ACNGT 2 cut(s) 328, 745
TaiI ACGT 3 cut(s) 249, 435, 642
TaqI TCGA 3 cut(s) 440, 816, 867
TaqII GACCGA 1 cut(s) 82
TasI AATT 1 cut(s) 632
TatI WGTACW 2 cut(s) 660, 804
TauI GCSGC 5 cut(s) 230, 599, 704, 734, 839
TfiI GAWTC 2 cut(s) 214, 672
Tru1I TTAA 1 cut(s) 874
Tru9I TTAA 1 cut(s) 874
TscAI CASTG 1 cut(s) 722
TseFI GTSAC 2 cut(s) 253, 481
TseI GCWGC 2 cut(s) 522, 525
Tsp45I GTSAC 2 cut(s) 253, 481
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 4 cut(s) 25, 151, 296, 603
TspRI CASTG 1 cut(s) 722
Tth111I GACNNNGTC 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 549
VneI GTGCAC 1 cut(s) 164
VpaK11BI GGWCC 3 cut(s) 345, 461, 552
XmiI GTMKAC 2 cut(s) 21, 135
ZrmI AGTACT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.