pycom04g16370

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0020412
Physical Location & Seq
Forward (+)
19044636 .. 19045484
849 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 243 bp
ATGAAGCTTTTTTGGGTTAGCATTGTAATTTCACTTGCTTGCAGCAAATGGGTTGCTGGGTTTATGGAGTACGGATACAATGAAACAGAGATTGCATTGTTGGAGGGCTACGGGGAGTCCAAGGTCGGAGCTAACTTGCTGATGGTCGGACTAACCCTCATTCGAGGAGCTGGAGCTAAACGAGCAGTCTGCTTGGATGGAACCTTACCGGGCTATCATTTGCATCGCGGATATGGATCATGA

Protein Analysis

81

Amino Acids

8.66

Weight (kDa)

7.79

Isoelectric Point (pI)

10.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 45 - 80 2e-09 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020412)

Species Orthologous Gene IDs
prunus_persica Prupe.6G303100_v2.0.a1
pyrus_communis pycom04g16370
rosa_chinensis RchiOBHm_Chr1g0328041
rosa_roxburghii Rroxscaffold_4G00289790
rosa_samantha Rh5AG130300
rosa_wichuraiana Rw5G016610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 228
AciI CCGC 1 cut(s) 228
AfaI GTAC 1 cut(s) 71
AluBI AGCT 4 cut(s) 7, 131, 170, 176
AluI AGCT 4 cut(s) 7, 131, 170, 176
ApeKI GCWGC 1 cut(s) 42
AsuC2I CCSGG 1 cut(s) 210
BbvI GCAGC 1 cut(s) 54
BccI CCATC 2 cut(s) 136, 191
BcgI CGANNNNNNTGC 2 cut(s) 171, 205
BciVI GTATCC 1 cut(s) 68
BcnI CCSGG 1 cut(s) 210
BfuI GTATCC 1 cut(s) 68
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 192
BpuMI CCSGG 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 120
Bse8I GATNNNNATC 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 1 cut(s) 202
BseJI GATNNNNATC 1 cut(s) 235
BseRI GAGGAG 1 cut(s) 180
BseXI GCAGC 1 cut(s) 54
BseYI CCCAGC 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 228
BsiSI CCGG 1 cut(s) 209
Bsp143I GATC 1 cut(s) 236
BspACI CCGC 1 cut(s) 228
BspFNI CGCG 1 cut(s) 228
BspHI TCATGA 1 cut(s) 239
BspLI GGNNCC 1 cut(s) 202
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 1 cut(s) 236
BssT1I CCWWGG 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 40
BstF5I GGATG 1 cut(s) 202
BstFNI CGCG 1 cut(s) 228
BstKTI GATC 1 cut(s) 239
BstMBI GATC 1 cut(s) 236
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 208
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 54
BsuI GTATCC 1 cut(s) 68
BtgZI GCGATG 1 cut(s) 209
BtsCI GGATG 1 cut(s) 202
Cac8I GCNNGC 1 cut(s) 40
CciI TCATGA 1 cut(s) 239
Csp6I GTAC 1 cut(s) 70
CviAII CATG 1 cut(s) 240
CviJI RGCY 6 cut(s) 7, 108, 131, 170, 176, 213
CviKI_1 RGCY 6 cut(s) 7, 108, 131, 170, 176, 213
CviQI GTAC 1 cut(s) 70
DpnI GATC 1 cut(s) 238
DpnII GATC 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 1 cut(s) 243
FaiI YATR 3 cut(s) 65, 234, 241
FatI CATG 1 cut(s) 239
Fnu4HI GCNGC 1 cut(s) 43
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 43
GluI GCNGC 1 cut(s) 43
GsaI CCCAGC 1 cut(s) 60
GsuI CTGGAG 1 cut(s) 192
HapII CCGG 1 cut(s) 209
Hin1II CATG 1 cut(s) 243
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 1 cut(s) 116
HpaII CCGG 1 cut(s) 209
Hpy188I TCNGA 2 cut(s) 128, 149
Hpy188III TCNNGA 1 cut(s) 240
HpyCH4V TGCA 3 cut(s) 42, 95, 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
Hsp92II CATG 1 cut(s) 243
Kzo9I GATC 1 cut(s) 236
LmnI GCTCC 3 cut(s) 128, 167, 173
LpnPI CCDG 3 cut(s) 42, 156, 222
Lsp1109I GCAGC 1 cut(s) 54
LweI GCATC 1 cut(s) 232
MalI GATC 1 cut(s) 238
MboI GATC 1 cut(s) 236
MluCI AATT 1 cut(s) 27
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 3 cut(s) 81, 106, 127
MnlI CCTC 3 cut(s) 97, 158, 167
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 210
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 1 cut(s) 210
NdeII GATC 1 cut(s) 236
NlaIII CATG 1 cut(s) 243
NlaIV GGNNCC 1 cut(s) 202
PagI TCATGA 1 cut(s) 239
PkrI GCNGC 1 cut(s) 44
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
PspFI CCCAGC 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 202
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 1 cut(s) 236
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 210
SetI ASST 6 cut(s) 9, 126, 133, 172, 178, 206
SfaNI GCATC 1 cut(s) 232
Sse9I AATT 1 cut(s) 27
SsiI CCGC 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 1 cut(s) 120
TaqI TCGA 1 cut(s) 163
TasI AATT 1 cut(s) 27
TseI GCWGC 1 cut(s) 42
TspDTI ATGAA 2 cut(s) 17, 96
TspGWI ACGGA 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.