Rh5AG130300

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0020412
Physical Location & Seq
Reverse (-)
12971057 .. 12974114
3058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG130300.1

Sequence Viewer

Length: 474 bp
ATGAGCAAGCTTTCAAAGATGAAGTTTTTGTGGGTTGGCATTGTTGTAGTGCTAGTTTTCACCAAATGGGCTAATGGGTTTGAGGAGCATAGTTTTAATGAAACAGAGCTGTCCTGGTTGGAGGGCTATGGAGAATCCAAGGCTTCTGTAACTGGGGTGATGGTAGGACTAACCCTTATTCCAGGAGCTGGTGCCAAAGGAGCAGTCTGCCTCGATGGAACCTTACCCAGGTATCATTTGCATCGTGGGTACGGGTCAGGAGCAAATAGCTGGCTTATTCAATTGGAGGGGGGAGGATGGTGTAACACCATTAGAAATTGTGTTTACCGCAAAACAACAAGGCGGGGATCATCAAAATTTATGGAAAAACAGTTGCTATTTACCGGAATACTAAGCAATAAAGCTGAAGAAAATCTAGGTTTGGGAATATCTAGTGAAATGTTGCCTAAAGAGTCACTAAGTGATCAAGCATAG

Protein Analysis

157

Amino Acids

17.21

Weight (kDa)

8.79

Isoelectric Point (pI)

25.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 52 - 138 8.8e-37 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020412)

Species Orthologous Gene IDs
prunus_persica Prupe.6G303100_v2.0.a1
pyrus_communis pycom04g16370
rosa_chinensis RchiOBHm_Chr1g0328041
rosa_roxburghii Rroxscaffold_4G00289790
rosa_samantha Rh5AG130300
rosa_wichuraiana Rw5G016610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 188
AciI CCGC 2 cut(s) 328, 343
AclWI GGATC 1 cut(s) 355
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 1 cut(s) 426
AdeI CACNNNGTG 1 cut(s) 461
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 2 cut(s) 188, 228
AgsI TTSAA 2 cut(s) 15, 281
AjnI CCWGG 3 cut(s) 113, 181, 227
AluBI AGCT 5 cut(s) 10, 109, 188, 270, 404
AluI AGCT 5 cut(s) 10, 109, 188, 270, 404
AlwI GGATC 1 cut(s) 355
AlwNI CAGNNNCTG 1 cut(s) 188
ApoI RAATTY 1 cut(s) 356
ArsI GACNNNNNNTTYG 2 cut(s) 189, 221
AsuHPI GGTGA 2 cut(s) 52, 169
BaeI ACNNNNGTAYC 2 cut(s) 215, 248
BanI GGYRCC 1 cut(s) 191
BccI CCATC 3 cut(s) 154, 209, 291
BciT130I CCWGG 3 cut(s) 115, 183, 229
BclI TGATCA 1 cut(s) 463
BfaI CTAG 3 cut(s) 53, 416, 432
Bme1390I CCNGG 3 cut(s) 115, 183, 229
BmiI GGNNCC 2 cut(s) 193, 220
BmrFI CCNGG 3 cut(s) 115, 183, 229
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 250
BmuI ACTGGG 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 138, 227
BsaWI WCCGGW 1 cut(s) 383
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 228
Bse1I ACTGG 1 cut(s) 157
BseBI CCWGG 3 cut(s) 115, 183, 229
BseDI CCNNGG 2 cut(s) 138, 227
BseGI GGATG 1 cut(s) 302
BseLI CCNNNNNNNGG 2 cut(s) 188, 228
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 98
BshNI GGYRCC 1 cut(s) 191
BsiSI CCGG 1 cut(s) 384
BslI CCNNNNNNNGG 2 cut(s) 188, 228
Bsp143I GATC 2 cut(s) 347, 463
BspACI CCGC 2 cut(s) 328, 343
BspLI GGNNCC 2 cut(s) 193, 220
BspPI GGATC 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 191
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 138, 227
BssMI GATC 2 cut(s) 347, 463
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 3 cut(s) 115, 183, 229
Bst4CI ACNGT 1 cut(s) 372
BstC8I GCNNGC 2 cut(s) 8, 272
BstDEI CTNAG 2 cut(s) 392, 458
BstENI CCTNNNNNAGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 302
BstKTI GATC 2 cut(s) 350, 466
BstMBI GATC 2 cut(s) 347, 463
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNI CCWGG 3 cut(s) 115, 183, 229
BstSCI CCNGG 3 cut(s) 113, 181, 227
BtsCI GGATG 1 cut(s) 302
Cac8I GCNNGC 2 cut(s) 8, 272
CaiI CAGNNNCTG 1 cut(s) 188
Csp6I GTAC 1 cut(s) 250
CviJI RGCY 9 cut(s) 10, 71, 109, 126, 143, 188, 270, 274, 404
CviKI_1 RGCY 9 cut(s) 10, 71, 109, 126, 143, 188, 270, 274, 404
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 2 cut(s) 392, 458
DpnI GATC 2 cut(s) 349, 465
DpnII GATC 2 cut(s) 347, 463
DraIII CACNNNGTG 1 cut(s) 461
Eco130I CCWWGG 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 226
EcoRII CCWGG 3 cut(s) 113, 181, 227
EcoT14I CCWWGG 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaiI YATR 4 cut(s) 90, 129, 362, 472
FauI CCCGC 1 cut(s) 336
FbaI TGATCA 1 cut(s) 463
FokI GGATG 1 cut(s) 309
FspBI CTAG 3 cut(s) 53, 416, 432
HapII CCGG 1 cut(s) 384
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 2 cut(s) 134, 452
HpaII CCGG 1 cut(s) 384
HphI GGTGA 2 cut(s) 52, 169
Hpy166II GTNNAC 1 cut(s) 325
Hpy188III TCNNGA 1 cut(s) 258
Hpy8I GTNNAC 1 cut(s) 325
HpyCH4III ACNGT 1 cut(s) 372
HpyCH4V TGCA 1 cut(s) 241
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 2 cut(s) 392, 458
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 2 cut(s) 347, 463
LmnI GCTCC 4 cut(s) 85, 185, 200, 260
LweI GCATC 1 cut(s) 250
MaeI CTAG 3 cut(s) 53, 416, 432
MaeIII GTNAC 3 cut(s) 148, 302, 453
MalI GATC 2 cut(s) 349, 465
MboI GATC 2 cut(s) 347, 463
MboII GAAGA 1 cut(s) 419
MfeI CAATTG 1 cut(s) 281
MluCI AATT 3 cut(s) 281, 316, 356
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 5 cut(s) 76, 115, 221, 280, 287
MseI TTAA 1 cut(s) 96
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 3 cut(s) 115, 183, 229
MunI CAATTG 1 cut(s) 281
MvaI CCWGG 3 cut(s) 115, 183, 229
MwoI GCNNNNNNNGC 1 cut(s) 200
NdeII GATC 2 cut(s) 347, 463
NlaIV GGNNCC 2 cut(s) 193, 220
NmuCI GTSAC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 134
PflMI CCANNNNNTGG 1 cut(s) 188
PfoI TCCNGGA 1 cut(s) 181
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
Psp6I CCWGG 3 cut(s) 113, 181, 227
PspGI CCWGG 3 cut(s) 113, 181, 227
PspN4I GGNNCC 2 cut(s) 193, 220
PstNI CAGNNNCTG 1 cut(s) 188
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SaqAI TTAA 1 cut(s) 96
Sau3AI GATC 2 cut(s) 347, 463
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 3 cut(s) 115, 183, 229
SetI ASST 8 cut(s) 12, 111, 190, 224, 233, 272, 406, 421
SfaNI GCATC 1 cut(s) 250
Sse9I AATT 3 cut(s) 281, 316, 356
SsiI CCGC 2 cut(s) 328, 343
SspMI CTAG 3 cut(s) 53, 416, 432
StyD4I CCNGG 3 cut(s) 113, 181, 227
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 372
TaqI TCGA 1 cut(s) 213
TasI AATT 3 cut(s) 281, 316, 356
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseFI GTSAC 1 cut(s) 453
Tsp45I GTSAC 1 cut(s) 453
TspDTI ATGAA 2 cut(s) 35, 114
Van91I CCANNNNNTGG 1 cut(s) 188
XagI CCTNNNNNAGG 1 cut(s) 226
XapI RAATTY 1 cut(s) 356
XspI CTAG 3 cut(s) 53, 416, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.