Rroxscaffold_4G00289790

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0020412
Physical Location & Seq
Reverse (-)
10431792 .. 10435194
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289790.1

Sequence Viewer

Length: 615 bp
ATGAGGACTTTGAGCTGGGGGCCGATTACGGTAGTCAGGGCTTTGAGTCCAGAAGGGTCGTTACGAGAGCTCGATGATTCACCGGTTTCAGTGGAGCTGGCGCCCTTCTGCAGCGAAGCCCAGTTTGATTGGCTAGTCGCTGAGGCTCAACAGCTCCAGCAGTTGTTCGTCATCATTTGGGCAAGCATTACTCCTAGAACTATCTTCATTATTTTGGCTGGAAGCTTCAAAAGCAACGAAATTATCCTTTTCAAGCACATAAATTGCAGTAAAGGAACAAGGACAATAAGTTTCAATCCAATTCAGGTGGTAGAGAGATTGAAACAGAGCAGACCCAAGATGAAGTTTTTGTGGGTTGGCATTGTTGTAGTGCTAGTTTTCACCAAATGGGCTAATGGGTTTGAGGAGCATAGTTTTAATGAAACAGAGCTGTCCTGGTTGGAGGGCTATGGAGAATCCAAGGCTGCTGTAACTGGGGTGATGGTAGGACTAACCCTTATTCCAGGAGCTGGTGCCAAAGGAGCAGTCTGCCTCGATGGAACCTTACCCAGGTTTCATTTGCATCGTGGGTACGGGTCAGGAGCAAATAGCTGGCTTATTCAATTGGAGGTGTGA

Protein Analysis

204

Amino Acids

22.6

Weight (kDa)

6.91

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 159 - 203 4.5e-14 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020412)

Species Orthologous Gene IDs
prunus_persica Prupe.6G303100_v2.0.a1
pyrus_communis pycom04g16370
rosa_chinensis RchiOBHm_Chr1g0328041
rosa_roxburghii Rroxscaffold_4G00289790
rosa_samantha Rh5AG130300
rosa_wichuraiana Rw5G016610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 100, 512
AccB7I CCANNNNNTGG 1 cut(s) 509
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 2 cut(s) 509, 549
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 5 cut(s) 229, 253, 295, 322, 602
AjnI CCWGG 3 cut(s) 434, 502, 548
AluBI AGCT 8 cut(s) 15, 70, 97, 154, 225, 430, 509, 591
AluI AGCT 8 cut(s) 15, 70, 97, 154, 225, 430, 509, 591
Alw21I GWGCWC 1 cut(s) 72
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 111, 464
ArsI GACNNNNNNTTYG 2 cut(s) 510, 542
AsiGI ACCGGT 1 cut(s) 82
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 3 cut(s) 72, 373, 490
BanI GGYRCC 2 cut(s) 100, 512
BanII GRGCYC 1 cut(s) 72
BarI GAAGNNNNNNTAC 2 cut(s) 45, 77
Bbv12I GWGCWC 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 2 cut(s) 123, 451
BccI CCATC 2 cut(s) 475, 530
BciT130I CCWGG 3 cut(s) 436, 504, 550
BfaI CTAG 3 cut(s) 134, 195, 374
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 1 cut(s) 104
BisI GCNGC 2 cut(s) 112, 465
BlsI GCNGC 2 cut(s) 113, 466
Bme1390I CCNGG 3 cut(s) 436, 504, 550
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 4 cut(s) 21, 102, 514, 541
BmrFI CCNGG 3 cut(s) 436, 504, 550
BmrI ACTGGG 2 cut(s) 115, 483
BmsI GCATC 1 cut(s) 571
BmuI ACTGGG 2 cut(s) 115, 483
BpmI CTGGAG 1 cut(s) 140
Bpu10I CCTNAGC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 459, 548
BsaWI WCCGGW 1 cut(s) 82
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 549
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 2 cut(s) 121, 478
BseBI CCWGG 3 cut(s) 436, 504, 550
BseDI CCNNGG 2 cut(s) 459, 548
BseLI CCNNNNNNNGG 2 cut(s) 509, 549
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 2 cut(s) 121, 478
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 123, 451
BseYI CCCAGC 1 cut(s) 15
BshFI GGCC 1 cut(s) 22
BshNI GGYRCC 2 cut(s) 100, 512
BshTI ACCGGT 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 509, 549
BsnI GGCC 1 cut(s) 22
Bsp1286I GDGCHC 1 cut(s) 72
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 133
BspLI GGNNCC 4 cut(s) 21, 102, 514, 541
BspMAI CTGCAG 1 cut(s) 113
BspT107I GGYRCC 2 cut(s) 100, 512
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 2 cut(s) 121, 478
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 459, 548
BssNI GRCGYC 1 cut(s) 101
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 3 cut(s) 436, 504, 550
Bst4CI ACNGT 1 cut(s) 31
BstACI GRCGYC 1 cut(s) 101
BstC8I GCNNGC 3 cut(s) 99, 184, 593
BstDEI CTNAG 1 cut(s) 141
BstENI CCTNNNNNAGG 1 cut(s) 547
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstMWI GCNNNNNNNGC 2 cut(s) 231, 521
BstNI CCWGG 3 cut(s) 436, 504, 550
BstSCI CCNGG 3 cut(s) 434, 502, 548
BstSFI CTRYAG 1 cut(s) 109
BstV1I GCAGC 2 cut(s) 123, 451
BsuRI GGCC 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 3 cut(s) 99, 184, 593
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 1 cut(s) 103
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 20
Csp6I GTAC 1 cut(s) 571
CspAI ACCGGT 1 cut(s) 82
CspCI CAANNNNNGTGG 2 cut(s) 288, 323
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 1 cut(s) 141
DinI GGCGCC 1 cut(s) 102
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 547
EcoRII CCWGG 3 cut(s) 434, 502, 548
EcoT14I CCWWGG 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 72
EgeI GGCGCC 1 cut(s) 102
EheI GGCGCC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 459
FaiI YATR 3 cut(s) 260, 411, 450
Fnu4HI GCNGC 2 cut(s) 112, 465
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 2 cut(s) 112, 465
FspBI CTAG 3 cut(s) 134, 195, 374
GlaI GCGC 1 cut(s) 102
GluI GCNGC 2 cut(s) 112, 465
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 1 cut(s) 22
HapII CCGG 1 cut(s) 83
HhaI GCGC 1 cut(s) 103
Hin1I GRCGYC 1 cut(s) 101
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HindIII AAGCTT 1 cut(s) 223
HinfI GANTC 3 cut(s) 46, 77, 455
HpaII CCGG 1 cut(s) 83
HphI GGTGA 3 cut(s) 72, 373, 490
Hpy188III TCNNGA 2 cut(s) 50, 579
HpyAV CCTTC 2 cut(s) 47, 115
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 111, 267, 562
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 521
HpyF3I CTNAG 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 101
HspAI GCGC 1 cut(s) 101
KasI GGCGCC 1 cut(s) 100
LmnI GCTCC 6 cut(s) 94, 159, 406, 506, 521, 581
Lsp1109I GCAGC 2 cut(s) 123, 451
LweI GCATC 1 cut(s) 571
MaeI CTAG 3 cut(s) 134, 195, 374
MaeIII GTNAC 2 cut(s) 60, 469
MboII GAAGA 1 cut(s) 196
MfeI CAATTG 1 cut(s) 602
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 4 cut(s) 240, 262, 300, 602
Mly113I GGCGCC 1 cut(s) 101
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 5 cut(s) 136, 397, 436, 542, 601
MseI TTAA 1 cut(s) 417
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 3 cut(s) 436, 504, 550
MunI CAATTG 1 cut(s) 602
MvaI CCWGG 3 cut(s) 436, 504, 550
MwoI GCNNNNNNNGC 2 cut(s) 231, 521
NarI GGCGCC 1 cut(s) 101
NlaIV GGNNCC 4 cut(s) 21, 102, 514, 541
PfeI GAWTC 2 cut(s) 77, 455
PflMI CCANNNNNTGG 1 cut(s) 509
PfoI TCCNGGA 1 cut(s) 502
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 2 cut(s) 113, 466
PleI GAGTC 1 cut(s) 54
PluTI GGCGCC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 3 cut(s) 434, 502, 548
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 3 cut(s) 434, 502, 548
PspN4I GGNNCC 4 cut(s) 21, 102, 514, 541
PspPI GGNCC 1 cut(s) 20
PstI CTGCAG 1 cut(s) 113
PstNI CAGNNNCTG 1 cut(s) 509
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 2 cut(s) 112, 465
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 3 cut(s) 436, 504, 550
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 1 cut(s) 109
SfoI GGCGCC 1 cut(s) 102
Sse9I AATT 4 cut(s) 240, 262, 300, 602
SspDI GGCGCC 1 cut(s) 100
SspMI CTAG 3 cut(s) 134, 195, 374
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 434, 502, 548
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 31
TaqI TCGA 2 cut(s) 72, 534
TasI AATT 4 cut(s) 240, 262, 300, 602
TfiI GAWTC 2 cut(s) 77, 455
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 1 cut(s) 96
TseI GCWGC 2 cut(s) 111, 464
TspDTI ATGAA 4 cut(s) 196, 356, 435, 545
TspRI CASTG 1 cut(s) 96
Van91I CCANNNNNTGG 1 cut(s) 509
XagI CCTNNNNNAGG 1 cut(s) 547
XspI CTAG 3 cut(s) 134, 195, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.