pycom04g19390

Protein MON2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007304
Physical Location & Seq
Reverse (-)
21115669 .. 21130273
14605 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4983 bp
ATGGCTTTCATGGCCGTTTTGGAGTCGGATCTTCGCGCGCTTTCCGCCGAAGCCCGCCGTCGCTACCCCGCGGTCAAGGACGGCGCTGAGCACGCAATTATCAAGCTTCGTAATTTGTCGAGTCCCGGTGAGATTGCGCAGAATGAGGACATACTGAGAATCTTTTTGATGGCTTGTGAGGTTAAAACTGTGAAGCTGAGTGTTATCGGACTTTCGTGCTTACAAAAGCTAATATCTCATGACGCTGTTGCTCCGTCAGCCCTGAATGAAATTCTTTCCACACTGAAGGATCATGCAGAAATGGCTGACGAAAGTGTTCAACTTAAGACCCTGCAGACTGTGTTAATCATATTGCAGTCACCTATACATCCCGAGACTGAGGACAGTATGGCTCAAGCTCTTGGTATCTGCCTTCGGCTGCTTGAAAACAACCGGTCCTCTGATAGTGTACGGAATACTGCTGCAGCTACTTTCAGGCAAGCGGTGGCACTGATTTTTGATCATGTGATTGGCGCTGAGACTCTTCCTGCTGGCAAGCTTAGTTCTGGAGGTTACATCTCTCGCACCAGTCTAGTTAGTGGCGATGTTAGTTCCAGTATCAATCTTTCAGAGTCATTGGATAAATCTCTTTCTGGACGATCAGCATTGATGAGGGAGACCTTAACTAAAGCTGGAAAGCTTGGACTTCGTCTGCTTGAAGACCTGACAGCTCTTGCTGCAGGTGGATCTGCAATATGGTTACGAGTGGGCTCTCTTCAGCGGTCCTTTGCACTTGATATACTGGAGTTTGTTTTGTCCAATTATGTGGCTGTTTTCAGAACCCTGCTTCCTTATGAGCAGGTTCTGCGCCACCAGATATGTTCACTTCTAATGACTTCACTTCGTACCAATGCTGAGATTGAAGGGGAAGCAGGGGAGCCTTCTTTTCGTCGTTTGGTCTTGCGGTCAGTTGCCCATATTATCAGGCTATACAGTTCATCCCTTATCACAGAATGTGAGGTTTTCCTTAGTATGTTGGTGAAGGCCACATTTCTTGATCTGCCATTGTGGCATCGTATTCTTGTTCTTGAGATCCTGAGGGGCTTTTGTGTGGACGCAAGGACGTTGCGGATTCTTTTCCAAAATTTTGATATGGATCCAAAGAACACCAATGTTGTGGAGGGCATGGTCAAGGCTCTTGCTAGAGTTGTTTCAAGTATACAGTTTCAAGAGACTAGTGATGAGAGCCTGGCTGCTGTTGCAGGGATGTTTAACAGCAAAGCTAAAGGAATCGAGTGGAGCCTTGATAACGATGCATCCAATGCTGCGGTCTTGGTTGCCAGTGAAGCTCATTCGATAACATTGGCAGTTGAAGGCCTTCTGGGCATTGTCTTTACTGTGGCTACACTGACGGATGAAGCAGTAGATTCTGGGGAGATTGAATCCCCCAGACATGATTATGATCCACCTGCAAAGTGTTCTGGAAATACTTCTATTCTCTGCCTCTCAATGGTTGATTCATTATGGTTGACTATTCTAGATGCATTGTCCTTTATTTTGTCAAGGTCACAAGGAGAGGCTATTGTGTTGGAAATACTGAAGGGATATCAGGCCTTCACCCAGGCATGCGGGGTTCTTCGTGCCGTAGAACCCTTGAATTCTTTTCTCGCATCCCTTTGCAAATTTACGATCAATTTTCCCATTGAATCAGAAAGAAGGAGCAGTACTTTACAATCTCCTGGATCAAAACGCTTTGAACTATTAGTTGATCAAAGGGAAAGCGTTGTTCTTACTCCCAAGAATGTCCAGGCCTTAAGAACTCTGTTCAACATTGCTCATCGACTACATAATGTATTGGGCCCATCCTGGGTTTTGGTGTTGGAAACCCTAGCAGCTCTGGACCGAGCAATCCATTCACCACATGCCACCACACAGGAGGTGTCCACGGGTGTGCCTAAGCTTACAAGGGAATCATCTGGTCAATCCAGCGACTTAAACATTCTTTCTTCTTTGAATTCTCAGCTGTTCGAGAGCTCAGCACTAATGCACATATCTGCAGTAAAGTCCCTTCTTTCTGCCCTATGCCAACTGTCACAGCAATGCATGGCTGGGATTACAAGTGGTTCTGTACCCACATCAAGTCAAAAAGTTGACAACATAAACTTTTCAGTGGAGAGAATAATATCCATCCTTGTCAATAATCTACATAGAGTGGAGCCATTGTGGGATCAAGTCATTAGCCACTTTCTTGAGCTTGCAGATAAATCTAACCAGCATTTACGTAATATGGCCCTTGATGCATTAGATGAATCGATATGTGCTGTTTTAGGTTCAGAACAGTTCCAGGACAGCACAACAACCAGATCTAGAGCATCTCAGAATATGGAAACTGGACTTACACAGTTGGGATCACTTGAATGTGCTGTCATATCGCCACTAAGGGTTCTTTATTTATCTACTGAAAGTGTTGAATTACGTGCTGGATCTTTGAAAATTCTTCTGCATGTTTTAGAGAGACATGGGGAGAAATTGCACTACAGTTGGCCGGATATTCTTGAACTGTTGAGGTCTGTAGCGGATTCTTCAGAGAAGGAACTTGTTACTCTGGCCTTTCAGAGCCTACGTGTGATTATGAATGATGGACTTTCAACTATCCCCGCAGACTGCCTTCCTGTATGTGTAGATGTGACTGGAGCATACAGTGCTCAAAAGACAGAACTGAATATAAGCTTGACAGCGATAGGCCTTCTATGGACAACAACCGACTTTATAGCCAAAGGTCTGATGCATGGGATTGGAGAGGAAAAGGAAACAGGGATTTCTGATGTGCAATTGGATGGTGAAAAGCCAAAAGAACAAGCATCCGATGTATCAGACAATGTTAATGACCAAGCTCCCTTGTTAAATGTAGTTGATCGTGACAGGTTGCTTTTCTCTGCTTTCTCGTTACTTCAGAAGCTTGGAGCTGATGAAAGGCCAGAGGTTAGAAATTCAGCCATCAGGACACTTTTTCAGACACTTGGAAGTCATGGGCAAAAACTTTCGAAAAGCATGTGGGAGGATTGCCTTTGGAATTACATTTTTCCTACACTAGATCGTGCTTCTCACATGGCTGAGACTTCATCAAAAGATGAATGGCATGGGAAAGAACTCGGAACTCGAGGAGGAAAAGCAGTTCATATGCTCATACATCATAGTCGCAACACGGCTCAGAAACAGTGGGATGAAACACTTGTACTAGTGCTTGGAGGCATAGCACGTATATTACGTTCATTCTTTCCTTTTCTTAGAAGCTTAAACAACTTTTGGTCAGGATGGGAGTCTTTGCTTTTATTTGTCAAGAACAGCATTTTAAATGGTAGCAAAGAGGTTGCTATTGCTGCAATAAATTGTTTACTGACACCCGTTCTTTCTCATTCTTCTAAGGGGAACCTGCCAAGGCCTTACCTTGAATCAGTTCTTGATGCCTACGAGGTTGTTCTCCAAAAGTCAACAAACCTCAGTGGCAATGCAGCTATCAAGGTGAAGCAGGAGATTTTGCACAGTCTCGGGGAACTTTATGTGCAAGCCCAGAGGATGTTTGACGATAATTTGTATCAGCAGTTGCTTGCAGTCATAGATTCAGCTGTTAAACAAGCCATAATTGGCAATGATAATTGTGACACTGAGTTTGGACATGTTCCACTTGTATTACGTACTGTGCTGGAGATCCTTCCGATGTTACGTCCAACAGAGCTCATTTCTTCCATGTGGCTTATTCTTCTTGGGGATTTTTTGCAATATCTGCCGAGATTACATTCTTCTGTACAAAAGGAGGAAGATGGCGCAGAGGAAGCCAGCACTAGTGACCAAGTTCCAGACGATCACGTGAGAATGAAACGTGAAATTCCTAATGGGACATCTTCCATATCTTCAAGCAGAGTAGAACGTTCTCCAAGTTCTGGATTAAAGACATCTGTAACAGCAGGCATCCCCAATTATATGTTTGCAGAAAAGCTTGTTCCCTTGCTGGTCGATCTTTTTCTACAGGCCCCAGCAGTTCAAAAGTATATCTTGTATTCTGAAATTATTCAAAGTCTTGGGAGGTGTATGACTACAAGAAGAGAAAATCCAGATGGTGCTCTTTGGAGATTAGCTGTTGAAGGCTTCAACCGCATTCTGGTTGATGATGCCAGCAAATCGGCTATAAATTCTGGACTAGATTCAGTTGCTAGTAAGCCTGCAAGAACACGTATATGGAAGGAAGTTGCCGATGTTTATGAAGTATTCCTTGTGGGTTACTGTGGACGAGCTCTTCCCTCTGATGATTCTTTCTCAACTGTGGATGTAAAAGCTGATGAGTCCCTTGAGATGACTGTCTTAGATGTACTCGGAGACAGGATTCTAAAGTCACCTATCGATGCACCTTCCGATATTTTGCATCGTCTAGTTTCCACCTTGGACCGATGCGCATCACGTACTTGTTCCTTACCTGTCGATTTTGTAGAACTTATGCCTTCCCACTGCAGCAGATTTTCCTTGAAATGTTTACAGAAGCTATTCTCCTTGAGCAGTTACGACAACAAATCCCATGATTGGAATTCAGCGAGATCTGAAGTGAGCAAAATTGCAGTCATGGTACTGATAACCAGATGCGAATACATCTTGAGCAGATTTCTAATTGACGAGAATGACTTAGGTGGACGCCCATTGCCGGCTGCAAGACTTGAAGAAATTATTTACGTTCTTGAAGAGTTGGCGCGTCTGATAATTCATTCAGACACTGCATCCGTTCTCCCCTTGAAGGCACATTTGAAAAGTGCCCTCGAAAAAGAAAAGAATCACGACATGCGTCCGCATCTGCTTGTGCTATTTCCCTCATTTTCCGAGCTCGTAGTATCAAGAGAAGCAAGAATACGAGGGTCAGTGCAAGTCTTATTCAGACTGATCTCCAGAGAATTGGGTCTCGACAGAGCTTCCATGAACGGGCAAAATCCGGAAGAATTTGTACCAAGGACACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7_N

Protein Analysis

1661

Amino Acids

183.07

Weight (kDa)

5.65

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 3 - 170 2.4e-38 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 230 - 385 6.1e-21 Mon2/Sec7/BIG1-like, HUS domain
Sec7-like_HDS PF09324 801 - 867 2.7e-06 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 873 - 1134 5.2e-48 C-terminal region of Mon2 protein
Mon2_C PF16206 1319 - 1577 1.7e-14 C-terminal region of Mon2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 710, 1456
Acc16I TGCGCA 2 cut(s) 138, 4429
Acc36I ACCTGC 4 cut(s) 710, 829, 1456, 3420
AccB7I CCANNNNNTGG 1 cut(s) 3920
AccI GTMKAC 1 cut(s) 1198
AccII CGCG 4 cut(s) 36, 38, 71, 4720
AccIII TCCGGA 1 cut(s) 4954
AclI AACGTT 1 cut(s) 3907
AcoI YGGCCR 2 cut(s) 12, 2522
AcuI CTGAAG 6 cut(s) 305, 740, 1598, 2547, 2915, 4593
AcvI CACGTG 1 cut(s) 3847
AcyI GRCGYC 1 cut(s) 4663
AfiI CCNNNNNNNGG 8 cut(s) 1489, 1846, 3920, 4138, 4554, 4672, 4762, 4944
AflII CTTAAG 2 cut(s) 323, 1792
AflIII ACRYGT 3 cut(s) 2602, 3655, 4208
AgeI ACCGGT 1 cut(s) 432
AhlI ACTAGT 3 cut(s) 1214, 3217, 3821
AjnI CCWGG 6 cut(s) 1227, 1599, 1717, 1785, 1844, 2322
AleI CACNNNNGTG 1 cut(s) 1928
AloI GAACNNNNNNTCC 4 cut(s) 811, 843, 3138, 3170
Alw21I GWGCWC 7 cut(s) 93, 2015, 2686, 3717, 4102, 4273, 4851
Alw26I GTCTC 9 cut(s) 368, 512, 650, 1205, 2488, 3089, 3530, 4347, 4928
AlwNI CAGNNNCTG 2 cut(s) 844, 4742
Ama87I CYCGRG 3 cut(s) 371, 3138, 3527
Aor13HI TCCGGA 1 cut(s) 4954
ApaI GGGCCC 1 cut(s) 1841
ArsI GACNNNNNNTTYG 4 cut(s) 3632, 3664, 4575, 4607
AsiGI ACCGGT 1 cut(s) 432
Asp700I GAANNNNTTC 6 cut(s) 315, 1356, 1468, 3435, 4047, 4517
AspLEI GCGC 9 cut(s) 38, 40, 86, 139, 515, 849, 3806, 4430, 4720
AspS9I GGNCC 8 cut(s) 435, 762, 1837, 1838, 1879, 2269, 4009, 4420
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 8 cut(s) 140, 351, 1030, 1588, 1887, 2831, 3514, 4362
AsuII TTCGAA 1 cut(s) 3023
AvaI CYCGRG 3 cut(s) 371, 3138, 3527
AvaII GGWCC 4 cut(s) 435, 762, 1879, 4420
AxyI CCTNAGG 1 cut(s) 1076
BaeGI GKGCMC 2 cut(s) 1841, 4783
BamHI GGATCC 1 cut(s) 1135
BanII GRGCYC 6 cut(s) 752, 1841, 2015, 3717, 4273, 4851
BarI GAAGNNNNNNTAC 8 cut(s) 1687, 1719, 2031, 2063, 4857, 4889, 4950, 4982
BbrPI CACGTG 1 cut(s) 3847
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 7 cut(s) 93, 2015, 2686, 3717, 4102, 4273, 4851
BccI CCATC 9 cut(s) 163, 1849, 2174, 2612, 2810, 2984, 3288, 3794, 4088
BceAI ACGGC 4 cut(s) 42, 97, 1607, 3201
BciT130I CCWGG 6 cut(s) 1229, 1601, 1719, 1787, 1846, 2324
BclI TGATCA 2 cut(s) 499, 1747
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 9 cut(s) 368, 512, 650, 1205, 2488, 3089, 3530, 4347, 4928
BcuI ACTAGT 3 cut(s) 1214, 3217, 3821
BfmI CTRYAG 8 cut(s) 332, 462, 717, 2034, 2515, 2550, 4004, 4483
BfoI RGCGCY 2 cut(s) 87, 516
BfrI CTTAAG 2 cut(s) 323, 1792
BfuAI ACCTGC 4 cut(s) 710, 829, 1456, 3420
BglI GCCNNNNNGGC 2 cut(s) 1048, 1362
BglII AGATCT 2 cut(s) 2342, 4568
BlpI GCTNAGC 2 cut(s) 87, 2014
BmcAI AGTACT 1 cut(s) 1705
Bme1390I CCNGG 7 cut(s) 126, 1229, 1601, 1719, 1787, 1846, 2324
Bme18I GGWCC 4 cut(s) 435, 762, 1879, 4420
BmeT110I CYCGRG 3 cut(s) 371, 3138, 3527
BmgT120I GGNCC 8 cut(s) 435, 762, 1837, 1838, 1879, 2269, 4009, 4420
BmiI GGNNCC 7 cut(s) 918, 1137, 1280, 1839, 2196, 3410, 4011
BmrFI CCNGG 7 cut(s) 126, 1229, 1601, 1719, 1787, 1846, 2324
BoxI GACNNNNGTC 1 cut(s) 4809
BpiI GAAGAC 1 cut(s) 705
BpmI CTGGAG 5 cut(s) 567, 803, 2691, 3704, 4894
Bpu10I CCTNAGC 1 cut(s) 1935
Bpu1102I GCTNAGC 2 cut(s) 87, 2014
Bpu14I TTCGAA 1 cut(s) 3023
BpuEI CTTGAG 6 cut(s) 378, 1088, 2249, 4346, 4546, 4645
BpuMI CCSGG 1 cut(s) 126
Bsa29I ATCGAT 2 cut(s) 2291, 4377
BsaAI YACGTR 8 cut(s) 2261, 2456, 2603, 3239, 3674, 3847, 4211, 4436
BsaBI GATNNNNATC 3 cut(s) 1134, 1440, 4429
BsaHI GRCGYC 1 cut(s) 4663
BsaI GGTCTC 2 cut(s) 650, 4928
BsaJI CCNNGG 7 cut(s) 69, 1599, 1845, 1923, 3416, 4416, 4970
BsaWI WCCGGW 2 cut(s) 432, 4954
BsaXI ACNNNNNCTCC 2 cut(s) 2186, 2216
Bsc4I CCNNNNNNNGG 8 cut(s) 1489, 1846, 3920, 4138, 4554, 4672, 4762, 4944
Bse118I RCCGGY 2 cut(s) 432, 4672
Bse1I ACTGG 6 cut(s) 567, 594, 786, 1320, 2374, 2674
Bse21I CCTNAGG 1 cut(s) 1076
Bse3DI GCAATG 5 cut(s) 1809, 2084, 3493, 3634, 4667
Bse8I GATNNNNATC 3 cut(s) 1134, 1440, 4429
BseAI TCCGGA 1 cut(s) 4954
BseBI CCWGG 6 cut(s) 1229, 1601, 1719, 1787, 1846, 2324
BseCI ATCGAT 2 cut(s) 2291, 4377
BseDI CCNNGG 7 cut(s) 69, 1599, 1845, 1923, 3416, 4416, 4970
BseJI GATNNNNATC 3 cut(s) 1134, 1440, 4429
BseLI CCNNNNNNNGG 8 cut(s) 1489, 1846, 3920, 4138, 4554, 4672, 4762, 4944
BseMI GCAATG 5 cut(s) 1809, 2084, 3493, 3634, 4667
BseNI ACTGG 6 cut(s) 567, 594, 786, 1320, 2374, 2674
BsePI GCGCGC 1 cut(s) 36
BseRI GAGGAG 1 cut(s) 3156
BseSI GKGCMC 2 cut(s) 1841, 4783
BseYI CCCAGC 2 cut(s) 2087, 4012
Bsh1236I CGCG 4 cut(s) 36, 38, 71, 4720
BshTI ACCGGT 1 cut(s) 432
BshVI ATCGAT 2 cut(s) 2291, 4377
BsiHKAI GWGCWC 7 cut(s) 93, 2015, 2686, 3717, 4102, 4273, 4851
BsiHKCI CYCGRG 3 cut(s) 371, 3138, 3527
BsiSI CCGG 5 cut(s) 126, 433, 2525, 4673, 4955
BslFI GGGAC 4 cut(s) 108, 2029, 3889, 4306
BslI CCNNNNNNNGG 8 cut(s) 1489, 1846, 3920, 4138, 4554, 4672, 4762, 4944
BsmAI GTCTC 9 cut(s) 368, 512, 650, 1205, 2488, 3089, 3530, 4347, 4928
BsmFI GGGAC 4 cut(s) 108, 2029, 3889, 4306
BsmI GAATGC 1 cut(s) 4134
Bso31I GGTCTC 2 cut(s) 650, 4928
BsoBI CYCGRG 3 cut(s) 371, 3138, 3527
Bsp119I TTCGAA 1 cut(s) 3023
Bsp120I GGGCCC 1 cut(s) 1837
Bsp13I TCCGGA 1 cut(s) 4954
Bsp1407I TGTACA 1 cut(s) 3784
Bsp1720I GCTNAGC 2 cut(s) 87, 2014
BspDI ATCGAT 2 cut(s) 2291, 4377
BspEI TCCGGA 1 cut(s) 4954
BspFNI CGCG 4 cut(s) 36, 38, 71, 4720
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 7 cut(s) 918, 1137, 1280, 1839, 2196, 3410, 4011
BspMAI CTGCAG 5 cut(s) 336, 466, 721, 2038, 4487
BspMI ACCTGC 4 cut(s) 710, 829, 1456, 3420
BspQI GCTCTTC 1 cut(s) 4278
BspT104I TTCGAA 1 cut(s) 3023
BspTI CTTAAG 2 cut(s) 323, 1792
BspTNI GGTCTC 2 cut(s) 650, 4928
BsrDI GCAATG 5 cut(s) 1809, 2084, 3493, 3634, 4667
BsrFI RCCGGY 2 cut(s) 432, 4672
BsrGI TGTACA 1 cut(s) 3784
BsrI ACTGG 6 cut(s) 567, 594, 786, 1320, 2374, 2674
BssAI RCCGGY 2 cut(s) 432, 4672
BssECI CCNNGG 7 cut(s) 69, 1599, 1845, 1923, 3416, 4416, 4970
BssHII GCGCGC 1 cut(s) 36
BssNAI GTATAC 1 cut(s) 1199
BssNI GRCGYC 1 cut(s) 4663
BssT1I CCWWGG 3 cut(s) 3416, 4416, 4970
Bst1107I GTATAC 1 cut(s) 1199
Bst2UI CCWGG 6 cut(s) 1229, 1601, 1719, 1787, 1846, 2324
Bst6I CTCTTC 5 cut(s) 528, 759, 4075, 4278, 4704
BstACI GRCGYC 1 cut(s) 4663
BstAFI CTTAAG 2 cut(s) 323, 1792
BstAPI GCANNNNNTGC 4 cut(s) 844, 1301, 2681, 3763
BstAUI TGTACA 1 cut(s) 3784
BstBAI YACGTR 8 cut(s) 2261, 2456, 2603, 3239, 3674, 3847, 4211, 4436
BstBI TTCGAA 1 cut(s) 3023
BstDSI CCRYGG 2 cut(s) 69, 1923
BstFNI CGCG 4 cut(s) 36, 38, 71, 4720
BstH2I RGCGCY 2 cut(s) 87, 516
BstHHI GCGC 9 cut(s) 38, 40, 86, 139, 515, 849, 3806, 4430, 4720
BstMAI GTCTC 9 cut(s) 368, 512, 650, 1205, 2488, 3089, 3530, 4347, 4928
BstNI CCWGG 6 cut(s) 1229, 1601, 1719, 1787, 1846, 2324
BstNSI RCATGY 6 cut(s) 1608, 1904, 2486, 3034, 3659, 4810
BstPAI GACNNNNGTC 1 cut(s) 4809
BstSCI CCNGG 7 cut(s) 124, 1227, 1599, 1717, 1785, 1844, 2322
BstSFI CTRYAG 8 cut(s) 332, 462, 717, 2034, 2515, 2550, 4004, 4483
BstSLI GKGCMC 2 cut(s) 1841, 4783
BstSNI TACGTA 2 cut(s) 2261, 3674
BstUI CGCG 4 cut(s) 36, 38, 71, 4720
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 8 cut(s) 28, 725, 1071, 1135, 2342, 2462, 3687, 4568
BstXI CCANNNNNNTGG 3 cut(s) 805, 1156, 4918
BstYI RGATCY 8 cut(s) 28, 725, 1071, 1135, 2342, 2462, 3687, 4568
BstZ17I GTATAC 1 cut(s) 1199
Bsu15I ATCGAT 2 cut(s) 2291, 4377
Bsu36I CCTNAGG 1 cut(s) 1076
BsuTUI ATCGAT 2 cut(s) 2291, 4377
BtgI CCRYGG 2 cut(s) 69, 1923
BtgZI GCGATG 1 cut(s) 597
BtsI GCAGTG 2 cut(s) 4480, 4740
BveI ACCTGC 4 cut(s) 710, 829, 1456, 3420
CaiI CAGNNNCTG 2 cut(s) 844, 4742
CciI TCATGA 1 cut(s) 238
CfoI GCGC 9 cut(s) 38, 40, 86, 139, 515, 849, 3806, 4430, 4720
Cfr10I RCCGGY 2 cut(s) 432, 4672
Cfr13I GGNCC 8 cut(s) 435, 762, 1837, 1838, 1879, 2269, 4009, 4420
Cfr42I CCGCGG 1 cut(s) 72
ClaI ATCGAT 2 cut(s) 2291, 4377
CseI GACGC 5 cut(s) 251, 1103, 4671, 4709, 4799
CspAI ACCGGT 1 cut(s) 432
CspCI CAANNNNNGTGG 2 cut(s) 1015, 1050
DraI TTTAAA 1 cut(s) 3333
EaeI YGGCCR 2 cut(s) 12, 2522
Eam1104I CTCTTC 5 cut(s) 528, 759, 4075, 4278, 4704
EarI CTCTTC 5 cut(s) 528, 759, 4075, 4278, 4704
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 4 cut(s) 2013, 3715, 4271, 4849
Eco105I TACGTA 2 cut(s) 2261, 3674
Eco130I CCWWGG 3 cut(s) 3416, 4416, 4970
Eco147I AGGCCT 5 cut(s) 1356, 1592, 1790, 2724, 3421
Eco24I GRGCYC 6 cut(s) 752, 1841, 2015, 3717, 4273, 4851
Eco31I GGTCTC 2 cut(s) 650, 4928
Eco32I GATATC 1 cut(s) 1586
Eco47I GGWCC 4 cut(s) 435, 762, 1879, 4420
Eco53kI GAGCTC 4 cut(s) 2013, 3715, 4271, 4849
Eco57I CTGAAG 6 cut(s) 305, 740, 1598, 2547, 2915, 4593
Eco72I CACGTG 1 cut(s) 3847
Eco81I CCTNAGG 1 cut(s) 1076
Eco88I CYCGRG 3 cut(s) 371, 3138, 3527
EcoICRI GAGCTC 4 cut(s) 2013, 3715, 4271, 4849
EcoO109I RGGNCCY 1 cut(s) 4009
EcoRI GAATTC 3 cut(s) 1636, 1993, 4558
EcoRII CCWGG 6 cut(s) 1227, 1599, 1717, 1785, 1844, 2322
EcoRV GATATC 1 cut(s) 1586
EcoT14I CCWWGG 3 cut(s) 3416, 4416, 4970
EcoT22I ATGCAT 5 cut(s) 1297, 1525, 2084, 2281, 2769
EcoT38I GRGCYC 6 cut(s) 752, 1841, 2015, 3717, 4273, 4851
ErhI CCWWGG 3 cut(s) 3416, 4416, 4970
FalI AAGNNNNNCTT 4 cut(s) 4016, 4048, 4233, 4265
FaqI GGGAC 4 cut(s) 108, 2029, 3889, 4306
FauI CCCGC 4 cut(s) 62, 76, 1601, 2644
FauNDI CATATG 1 cut(s) 3159
FbaI TGATCA 2 cut(s) 499, 1747
FblI GTMKAC 1 cut(s) 1198
FriOI GRGCYC 6 cut(s) 752, 1841, 2015, 3717, 4273, 4851
FspAI RTGCGCAY 1 cut(s) 4429
FspI TGCGCA 2 cut(s) 138, 4429
GlaI GCGC 9 cut(s) 37, 39, 85, 138, 514, 848, 3805, 4429, 4719
GsaI CCCAGC 2 cut(s) 2091, 4016
GsuI CTGGAG 5 cut(s) 567, 803, 2691, 3704, 4894
HaeII RGCGCY 2 cut(s) 87, 516
HapII CCGG 5 cut(s) 126, 433, 2525, 4673, 4955
HgaI GACGC 5 cut(s) 251, 1103, 4671, 4709, 4799
HhaI GCGC 9 cut(s) 38, 40, 86, 139, 515, 849, 3806, 4430, 4720
Hin1I GRCGYC 1 cut(s) 4663
Hin6I GCGC 9 cut(s) 36, 38, 84, 137, 513, 847, 3804, 4428, 4718
HinP1I GCGC 9 cut(s) 36, 38, 84, 137, 513, 847, 3804, 4428, 4718
HincII GTYRAC 3 cut(s) 1509, 2131, 3471
HindII GTYRAC 3 cut(s) 1509, 2131, 3471
HindIII AAGCTT 8 cut(s) 104, 536, 677, 1937, 2707, 2936, 3271, 3974
HpaII CCGG 5 cut(s) 126, 433, 2525, 4673, 4955
HphI GGTGA 8 cut(s) 140, 351, 1030, 1588, 1887, 2831, 3514, 4362
Hpy99I CGWCG 2 cut(s) 63, 933
Hsp92I GRCGYC 1 cut(s) 4663
HspAI GCGC 9 cut(s) 36, 38, 84, 137, 513, 847, 3804, 4428, 4718
Kpn2I TCCGGA 1 cut(s) 4954
KroI GCCGGC 1 cut(s) 4672
KroNI GCCGGC 1 cut(s) 4674
Ksp22I TGATCA 2 cut(s) 499, 1747
KspI CCGCGG 1 cut(s) 72
LguI GCTCTTC 1 cut(s) 4278
LmnI GCTCC 8 cut(s) 256, 916, 1278, 1698, 2194, 2672, 2878, 2942
MfeI CAATTG 1 cut(s) 2810
MflI RGATCY 8 cut(s) 28, 725, 1071, 1135, 2342, 2462, 3687, 4568
MlyI GAGTC 6 cut(s) 32, 130, 514, 620, 3308, 4328
MmeI TCCRAC 4 cut(s) 6, 1548, 1839, 3731
Mph1103I ATGCAT 5 cut(s) 1297, 1525, 2084, 2281, 2769
MroI TCCGGA 1 cut(s) 4954
MroNI GCCGGC 1 cut(s) 4672
MroXI GAANNNNTTC 6 cut(s) 315, 1356, 1468, 3435, 4047, 4517
MslI CAYNNNNRTG 5 cut(s) 1437, 1928, 2077, 2395, 4213
MspA1I CMGCKG 4 cut(s) 71, 760, 2002, 3605
MspCI CTTAAG 2 cut(s) 323, 1792
MspI CCGG 5 cut(s) 126, 433, 2525, 4673, 4955
MspR9I CCNGG 7 cut(s) 126, 1229, 1601, 1719, 1787, 1846, 2324
MunI CAATTG 1 cut(s) 2810
Mva1269I GAATGC 1 cut(s) 4134
MvaI CCWGG 6 cut(s) 1229, 1601, 1719, 1787, 1846, 2324
MvnI CGCG 4 cut(s) 36, 38, 71, 4720
NaeI GCCGGC 1 cut(s) 4674
NciI CCSGG 1 cut(s) 126
NdeI CATATG 1 cut(s) 3159
NgoMIV GCCGGC 1 cut(s) 4672
NlaIV GGNNCC 7 cut(s) 918, 1137, 1280, 1839, 2196, 3410, 4011
NmeAIII GCCGAG 1 cut(s) 3792
NmuCI GTSAC 8 cut(s) 357, 1545, 2070, 2665, 2897, 3638, 3824, 4368
NsbI TGCGCA 2 cut(s) 138, 4429
NsiI ATGCAT 5 cut(s) 1297, 1525, 2084, 2281, 2769
NspI RCATGY 6 cut(s) 1608, 1904, 2486, 3034, 3659, 4810
NspV TTCGAA 1 cut(s) 3023
OliI CACNNNNGTG 1 cut(s) 1928
PaeI GCATGC 1 cut(s) 1608
PaeR7I CTCGAG 1 cut(s) 3138
PagI TCATGA 1 cut(s) 238
PaqCI CACCTGC 2 cut(s) 710, 1456
PauI GCGCGC 1 cut(s) 36
PceI AGGCCT 5 cut(s) 1356, 1592, 1790, 2724, 3421
PciI ACATGT 1 cut(s) 3655
PciSI GCTCTTC 1 cut(s) 4278
PcsI WCGNNNNNNNCGW 1 cut(s) 3244
PctI GAATGC 1 cut(s) 4134
PdiI GCCGGC 1 cut(s) 4674
PdmI GAANNNNTTC 6 cut(s) 315, 1356, 1468, 3435, 4047, 4517
PflFI GACNNNGTC 1 cut(s) 687
PflMI CCANNNNNTGG 1 cut(s) 3920
PfoI TCCNGGA 2 cut(s) 1717, 2322
PinAI ACCGGT 1 cut(s) 432
PleI GAGTC 6 cut(s) 31, 129, 514, 619, 3307, 4327
PmaCI CACGTG 1 cut(s) 3847
PmlI CACGTG 1 cut(s) 3847
PpsI GAGTC 6 cut(s) 31, 129, 514, 619, 3307, 4327
Ppu21I YACGTR 8 cut(s) 2261, 2456, 2603, 3239, 3674, 3847, 4211, 4436
PscI ACATGT 1 cut(s) 3655
PshAI GACNNNNGTC 1 cut(s) 4809
Psp124BI GAGCTC 4 cut(s) 2015, 3717, 4273, 4851
Psp1406I AACGTT 1 cut(s) 3907
Psp6I CCWGG 6 cut(s) 1227, 1599, 1717, 1785, 1844, 2322
PspCI CACGTG 1 cut(s) 3847
PspFI CCCAGC 2 cut(s) 2087, 4012
PspGI CCWGG 6 cut(s) 1227, 1599, 1717, 1785, 1844, 2322
PspN4I GGNNCC 7 cut(s) 918, 1137, 1280, 1839, 2196, 3410, 4011
PspOMI GGGCCC 1 cut(s) 1837
PspPI GGNCC 8 cut(s) 435, 762, 1837, 1838, 1879, 2269, 4009, 4420
PspXI VCTCGAGB 1 cut(s) 3138
PsrI GAACNNNNNNTAC 2 cut(s) 3232, 3264
PstI CTGCAG 5 cut(s) 336, 466, 721, 2038, 4487
PstNI CAGNNNCTG 2 cut(s) 844, 4742
PsuI RGATCY 8 cut(s) 28, 725, 1071, 1135, 2342, 2462, 3687, 4568
PsyI GACNNNGTC 1 cut(s) 687
PteI GCGCGC 1 cut(s) 36
PvuII CAGCTG 2 cut(s) 2002, 3605
RseI CAYNNNNRTG 5 cut(s) 1437, 1928, 2077, 2395, 4213
SacI GAGCTC 4 cut(s) 2015, 3717, 4273, 4851
SacII CCGCGG 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 4278
Sau96I GGNCC 8 cut(s) 435, 762, 1837, 1838, 1879, 2269, 4009, 4420
ScaI AGTACT 1 cut(s) 1705
SchI GAGTC 6 cut(s) 32, 130, 514, 620, 3308, 4328
ScrFI CCNGG 7 cut(s) 126, 1229, 1601, 1719, 1787, 1846, 2324
SfcI CTRYAG 8 cut(s) 332, 462, 717, 2034, 2515, 2550, 4004, 4483
Sfr274I CTCGAG 1 cut(s) 3138
Sfr303I CCGCGG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 3023
SgrBI CCGCGG 1 cut(s) 72
SinI GGWCC 4 cut(s) 435, 762, 1879, 4420
SlaI CTCGAG 1 cut(s) 3138
SmiMI CAYNNNNRTG 5 cut(s) 1437, 1928, 2077, 2395, 4213
SmlI CTYRAG 9 cut(s) 323, 393, 1067, 1792, 2228, 3138, 4325, 4525, 4624
SmoI CTYRAG 9 cut(s) 323, 393, 1067, 1792, 2228, 3138, 4325, 4525, 4624
SnaBI TACGTA 2 cut(s) 2261, 3674
SpeI ACTAGT 3 cut(s) 1214, 3217, 3821
SphI GCATGC 1 cut(s) 1608
SseBI AGGCCT 5 cut(s) 1356, 1592, 1790, 2724, 3421
SstI GAGCTC 4 cut(s) 2015, 3717, 4273, 4851
StuI AGGCCT 5 cut(s) 1356, 1592, 1790, 2724, 3421
StyD4I CCNGG 7 cut(s) 124, 1227, 1599, 1717, 1785, 1844, 2322
StyI CCWWGG 3 cut(s) 3416, 4416, 4970
TaqII GACCGA 2 cut(s) 1896, 4437
TatI WGTACW 4 cut(s) 1703, 3214, 3784, 4345
TseFI GTSAC 8 cut(s) 357, 1545, 2070, 2665, 2897, 3638, 3824, 4368
Tsp45I GTSAC 8 cut(s) 357, 1545, 2070, 2665, 2897, 3638, 3824, 4368
TspGWI ACGGA 4 cut(s) 243, 466, 1406, 4738
Tth111I GACNNNGTC 1 cut(s) 687
Van91I CCANNNNNTGG 1 cut(s) 3920
Vha464I CTTAAG 2 cut(s) 323, 1792
VpaK11BI GGWCC 4 cut(s) 435, 762, 1879, 4420
XbaI TCTAGA 2 cut(s) 1516, 2345
XceI RCATGY 6 cut(s) 1608, 1904, 2486, 3034, 3659, 4810
XhoI CTCGAG 1 cut(s) 3138
XmiI GTMKAC 1 cut(s) 1198
XmnI GAANNNNTTC 6 cut(s) 315, 1356, 1468, 3435, 4047, 4517
ZrmI AGTACT 1 cut(s) 1705
Zsp2I ATGCAT 5 cut(s) 1297, 1525, 2084, 2281, 2769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.