Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0016959

Physical Location & Seq

Genomic Coordinates Forward (+)

22498378 .. 22500785

Gene Structure 2408 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g21220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGCAGATGTTGGGGCGTGCAGGTAGACCTCAGTACGATTCGTATGGAGAAGGGATTATCATTACTGGCCACAATGAGCTTCAATACTATCTTTCTTTGATGAACCAGCAGCTTCCCATTGAAAGTCAATTCATATCCAAATTGGCTGACCAACTTAATGCTGAAATCGTTCTTGGAACTGTTCAAAATGCTAGAGAAGCTTGCAATTGGATAGGATACACTTACCTGTATGTTCGCATGCTACGGAACCCCACACTCTATGGTTTGGAAGCTGATGTTCTCTCCAGAGATATTACATTGGAGGAGAGACGAGCTGATTTGATCCATTCCGCTGCAACCATCTTGGACAAGAATAATTTGATTAAGTACGACAGAAAAAGTGGATATTTCCAAGTTACAGACTTGGGTCGCATTGCGAGTTATTACTATATAACTCATGGAACAATATCCACATATAATGAGCATTTGAAGCCTACGATGGGGGATATTGAGCTTTGTCGATTGTTCTCGCTCAGTGAAGAATTTAAGTATGTTACTGTGCGGCAGGATGAAAAGATGGAGTTAGCAAAGCTTTTGGATCGATCAAATGTTCCCATTCCGGTGAAGGAAAGCCTGGAAGAGCCCAGTGCCAAGATCAATGTCTTGCTGCAAGCATATATCTCACAGCTGCAGCTTGAGGGGCTTTCATTGACATCCGATATGGTCTACATTACTCAGAGTGCAGGGCGTCTTCTACGAGCTCTTTTTGAGATTGTCTTGAAACGAGGATGGGCTCAACTAGCTGAAAAGGCTTTGAACATGTGTAAGATGGTTAACAAGAAGATGTGGAGTGTCCAAACACCTCTTCGCCAATTCACTGGTATTTCAAATGATATTTTGATGAAGCTGGAGAAGAAGGATTTGGCCTGGGATAGGTATTATGATCTATCTTCACAGGAGCTAGGGGAGCTTATTCGTATGCCAAAGATGGGAAGAACACTTCATAAGTTCATCCACCAATTCCCCAAATTAAACCTTGCAGCCCATGTGCAGCCGATTACTCGCACTGTATTGAGGGTGGAGCTCACTATAACACCAGATTTCCAATGGGAGGACAAAGTTCATGGATATGTGGAGCCATTTTGGGTAATAGTGGAGGATAATGATGGCGAGTTTGTCCTTCATCATGAATATTTTCTTCTGAAGAAGCAGTATATTGATGAGGATCATACTTTGAACTTTACAGTGCCAATATATGAGCCTCTTCCGCCTCAGTACTTCATTCGTGTTGTGTCTGATAGGTGGCTTGGGTCCCAGACTGTTTTACCTGTTTCTTTCAGACACCTCATTTTACCTGAAAAGTATCCTCCACCAACAGAGTTATTGGACTTGCAACCACTTCCCGTGACTGCATTAAGGAATCCCTTATATGAAGCTCTGTATCAAGATTTCAAGCATTTCAATCCTGTTCAAACTCAGGTTTTCACTGTTTTGTACAATTCAGATGACAATGTCTTAGTTGCTGCACCAACAGGGAGTGGGAAGACCATATGTGCAGAGTTTGCTGTATTGAGGAATCATCAGAAGGGCTCTGATAATGTCATGCGTGTTGTGTATATTGCACCTATTGAAGCTCTTGCTAAGGAACGCTACCGTGACTGGGAGAAGAAGTTTGGAAAGGGTCTCAATCTGCGAGTTGAATTATTAACAGGGGAAACAGCCACAGACTTAAAACTGCTTGAGAAAGGTCAGATTATCATCAGCACTCCAGAGAAATGGGATGCTTTATCCCGCCGCTGGAAACAGAGAAAGCATGTTCAACAGGTTAGTTTTTTATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000354 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009506 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034622 GO:0034641 GO:0042623 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0046540 GO:0055044 GO:0065003 GO:0070013 GO:0070035 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:0140098 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C Sec63

Protein Analysis

607

Amino Acids

70.21

Weight (kDa)

6.76

Isoelectric Point (pI)

38.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_SNRNP200	PF23445	41 - 117	8.1e-25	Winged Helix-turn-helix domain
Sec63	PF02889	133 - 438	2.1e-99	Sec63 Brl domain
DEAD	PF00270	481 - 605	4e-23	DEAD/DEAH box helicase
ResIII	PF04851	481 - 600	2e-08	Type III restriction enzyme, res subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016959)

Species	Orthologous Gene IDs
pyrus_communis	pycom04g21220 pycom12g23610
rosa_chinensis	RchiOBHm_Chr3g0448021
rosa_multiflora	Rmu_sc0005582.1_g000010 Rmu_sc0005969.1_g000017
rosa_rugosa	Rorug02G0607800
rosa_samantha	Rh3AG006900 Rh3AG007000 Rh3BG006600 Rh3BG006700 Rh3CG006800 Rh3CG006900 Rh3DG007200 Rh3DG007300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	11
AccI	GTMKAC	2 cut(s)	25, 705
AciI	CCGC	5 cut(s)	330, 541, 1247, 1771, 1774
AclWI	GGATC	3 cut(s)	316, 585, 1212
AcoI	YGGCCR	1 cut(s)	67
AcsI	RAATTY	1 cut(s)	521
AcuI	CTGAAG	1 cut(s)	1202
AcyI	GRCGYC	1 cut(s)	727
AfaI	GTAC	4 cut(s)	35, 368, 1256, 1475
AfiI	CCNNNNNNNGG	6 cut(s)	479, 912, 968, 1090, 1639, 1776
AflIII	ACRYGT	1 cut(s)	798
AjnI	CCWGG	2 cut(s)	612, 905
AjuI	GAANNNNNNNTTGG	6 cut(s)	156, 188, 828, 860, 884, 916
Alw21I	GWGCWC	2 cut(s)	742, 1065
Alw26I	GTCTC	2 cut(s)	301, 1667
AlwI	GGATC	3 cut(s)	316, 585, 1212
AoxI	GGCC	2 cut(s)	67, 903
ApeKI	GCWGC	8 cut(s)	109, 332, 646, 667, 670, 1019, 1030, 1502
ApoI	RAATTY	1 cut(s)	521
Asp700I	GAANNNNTTC	2 cut(s)	168, 1173
AspS9I	GGNCC	1 cut(s)	1290
AsuHPI	GGTGA	1 cut(s)	613
AvaII	GGWCC	1 cut(s)	1290
BalI	TGGCCA	1 cut(s)	69
BanII	GRGCYC	5 cut(s)	624, 742, 775, 1065, 1571
BbsI	GAAGAC	2 cut(s)	722, 1529
Bbv12I	GWGCWC	2 cut(s)	742, 1065
BbvI	GCAGC	8 cut(s)	121, 319, 633, 654, 682, 1031, 1042, 1489
BccI	CCATC	7 cut(s)	347, 472, 550, 762, 802, 961, 1139
BciT130I	CCWGG	2 cut(s)	614, 907
BciVI	GTATCC	2 cut(s)	209, 1353
BcoDI	GTCTC	2 cut(s)	301, 1667
BfaI	CTAG	3 cut(s)	192, 779, 941
BfmI	CTRYAG	1 cut(s)	668
BfuAI	ACCTGC	1 cut(s)	11
BfuI	GTATCC	2 cut(s)	209, 1353
BmcAI	AGTACT	1 cut(s)	1256
Bme1390I	CCNGG	2 cut(s)	614, 907
Bme18I	GGWCC	1 cut(s)	1290
BmgT120I	GGNCC	1 cut(s)	1290
BmiI	GGNNCC	4 cut(s)	248, 1116, 1291, 1292
BmrFI	CCNGG	2 cut(s)	614, 907
BmrI	ACTGGG	2 cut(s)	618, 1648
BmsI	GCATC	1 cut(s)	1750
BmuI	ACTGGG	2 cut(s)	618, 1648
BoxI	GACNNNNGTC	1 cut(s)	405
BpiI	GAAGAC	2 cut(s)	722, 1529
BpmI	CTGGAG	3 cut(s)	268, 908, 1731
Bpu10I	CCTNAGC	1 cut(s)	1620
BpuEI	CTTGAG	2 cut(s)	695, 1739
Bsa29I	ATCGAT	1 cut(s)	580
BsaBI	GATNNNNATC	2 cut(s)	1203, 1736
BsaHI	GRCGYC	1 cut(s)	727
BsaI	GGTCTC	1 cut(s)	1667
BsaJI	CCNNGG	1 cut(s)	906
BsaWI	WCCGGW	1 cut(s)	598
BsaXI	ACNNNNNCTCC	2 cut(s)	1634, 1664
Bsc4I	CCNNNNNNNGG	6 cut(s)	479, 912, 968, 1090, 1639, 1776
Bse1I	ACTGG	4 cut(s)	70, 624, 862, 1643
Bse3DI	GCAATG	1 cut(s)	411
Bse8I	GATNNNNATC	2 cut(s)	1203, 1736
BseBI	CCWGG	2 cut(s)	614, 907
BseCI	ATCGAT	1 cut(s)	580
BseDI	CCNNGG	1 cut(s)	906
BseGI	GGATG	5 cut(s)	553, 692, 773, 990, 1765
BseJI	GATNNNNATC	2 cut(s)	1203, 1736
BseLI	CCNNNNNNNGG	6 cut(s)	479, 912, 968, 1090, 1639, 1776
BseMI	GCAATG	1 cut(s)	411
BseMII	CTCAG	5 cut(s)	44, 526, 728, 1265, 1469
BseNI	ACTGG	4 cut(s)	70, 624, 862, 1643
BseRI	GAGGAG	1 cut(s)	317
BseXI	GCAGC	8 cut(s)	121, 319, 633, 654, 682, 1031, 1042, 1489
BsgI	GTGCAG	5 cut(s)	39, 741, 1049, 1488, 1554
BshFI	GGCC	2 cut(s)	69, 905
BshVI	ATCGAT	1 cut(s)	580
BsiHKAI	GWGCWC	2 cut(s)	742, 1065
BsiSI	CCGG	1 cut(s)	599
BslFI	GGGAC	1 cut(s)	1276
BslI	CCNNNNNNNGG	6 cut(s)	479, 912, 968, 1090, 1639, 1776
BsmAI	GTCTC	2 cut(s)	301, 1667
BsmBI	CGTCTC	1 cut(s)	301
BsmFI	GGGAC	1 cut(s)	1276
BsnI	GGCC	2 cut(s)	69, 905
Bso31I	GGTCTC	1 cut(s)	1667
Bsp1286I	GDGCHC	5 cut(s)	624, 742, 775, 1065, 1571
Bsp1407I	TGTACA	1 cut(s)	1473
Bsp143I	GATC	6 cut(s)	321, 577, 581, 633, 922, 1204
BspACI	CCGC	5 cut(s)	330, 541, 1247, 1771, 1774
BspANI	GGCC	2 cut(s)	69, 905
BspCNI	CTCAG	5 cut(s)	43, 525, 727, 1264, 1468
BspDI	ATCGAT	1 cut(s)	580
BspHI	TCATGA	1 cut(s)	1165
BspLI	GGNNCC	4 cut(s)	248, 1116, 1291, 1292
BspMAI	CTGCAG	1 cut(s)	672
BspMI	ACCTGC	1 cut(s)	11
BspPI	GGATC	3 cut(s)	316, 585, 1212
BspQI	GCTCTTC	1 cut(s)	612
BspTNI	GGTCTC	1 cut(s)	1667
BsrDI	GCAATG	1 cut(s)	411
BsrGI	TGTACA	1 cut(s)	1473
BsrI	ACTGG	4 cut(s)	70, 624, 862, 1643
BssECI	CCNNGG	1 cut(s)	906
BssMI	GATC	6 cut(s)	321, 577, 581, 633, 922, 1204
BssNI	GRCGYC	1 cut(s)	727
Bst2UI	CCWGG	2 cut(s)	614, 907
Bst4CI	ACNGT	7 cut(s)	181, 538, 1048, 1225, 1300, 1468, 1634
Bst6I	CTCTTC	3 cut(s)	612, 849, 1248
BstACI	GRCGYC	1 cut(s)	727
BstAPI	GCANNNNNTGC	1 cut(s)	1541
BstAUI	TGTACA	1 cut(s)	1473
BstC8I	GCNNGC	4 cut(s)	18, 202, 239, 651
BstDEI	CTNAG	7 cut(s)	30, 512, 714, 1251, 1455, 1495, 1620
BstENI	CCTNNNNNAGG	1 cut(s)	910
BstF5I	GGATG	5 cut(s)	553, 692, 773, 990, 1765
BstKTI	GATC	6 cut(s)	324, 580, 584, 636, 925, 1207
BstMAI	GTCTC	2 cut(s)	301, 1667
BstMBI	GATC	6 cut(s)	321, 577, 581, 633, 922, 1204
BstMWI	GCNNNNNNNGC	8 cut(s)	197, 469, 679, 779, 788, 946, 1246, 1541
BstNI	CCWGG	2 cut(s)	614, 907
BstNSI	RCATGY	3 cut(s)	241, 802, 1796
BstPAI	GACNNNNGTC	1 cut(s)	405
BstSCI	CCNGG	2 cut(s)	612, 905
BstSFI	CTRYAG	1 cut(s)	668
BstV1I	GCAGC	8 cut(s)	121, 319, 633, 654, 682, 1031, 1042, 1489
BstV2I	GAAGAC	2 cut(s)	722, 1529
BstXI	CCANNNNNNTGG	2 cut(s)	857, 1755
Bsu15I	ATCGAT	1 cut(s)	580
BsuI	GTATCC	2 cut(s)	209, 1353
BsuRI	GGCC	2 cut(s)	69, 905
BsuTUI	ATCGAT	1 cut(s)	580
BtsCI	GGATG	5 cut(s)	553, 692, 773, 990, 1765
BtsIMutI	CAGTG	6 cut(s)	520, 631, 855, 1044, 1230, 1464
BveI	ACCTGC	1 cut(s)	11
Cac8I	GCNNGC	4 cut(s)	18, 202, 239, 651
CciI	TCATGA	1 cut(s)	1165
Cfr13I	GGNCC	1 cut(s)	1290
ClaI	ATCGAT	1 cut(s)	580
CseI	GACGC	1 cut(s)	716
Csp6I	GTAC	4 cut(s)	34, 367, 1255, 1474
CviAII	CATG	8 cut(s)	238, 437, 799, 1025, 1103, 1166, 1582, 1793
CviQI	GTAC	4 cut(s)	34, 367, 1255, 1474
DdeI	CTNAG	7 cut(s)	30, 512, 714, 1251, 1455, 1495, 1620
DpnI	GATC	6 cut(s)	323, 579, 583, 635, 924, 1206
DpnII	GATC	6 cut(s)	321, 577, 581, 633, 922, 1204
EaeI	YGGCCR	1 cut(s)	67
Eam1104I	CTCTTC	3 cut(s)	612, 849, 1248
EarI	CTCTTC	3 cut(s)	612, 849, 1248
EciI	GGCGGA	1 cut(s)	1236
Ecl136II	GAGCTC	2 cut(s)	740, 1063
Eco24I	GRGCYC	5 cut(s)	624, 742, 775, 1065, 1571
Eco31I	GGTCTC	1 cut(s)	1667
Eco47I	GGWCC	1 cut(s)	1290
Eco53kI	GAGCTC	2 cut(s)	740, 1063
Eco57I	CTGAAG	1 cut(s)	1202
EcoICRI	GAGCTC	2 cut(s)	740, 1063
EcoNI	CCTNNNNNAGG	1 cut(s)	910
EcoO109I	RGGNCCY	1 cut(s)	1290
EcoRII	CCWGG	2 cut(s)	612, 905
EcoT38I	GRGCYC	5 cut(s)	624, 742, 775, 1065, 1571
Esp3I	CGTCTC	1 cut(s)	301
FaeI	CATG	8 cut(s)	241, 440, 802, 1028, 1106, 1169, 1585, 1796
FaqI	GGGAC	1 cut(s)	1276
FatI	CATG	8 cut(s)	237, 436, 798, 1024, 1102, 1165, 1581, 1792
FauI	CCCGC	1 cut(s)	1778
FauNDI	CATATG	1 cut(s)	1529
FblI	GTMKAC	2 cut(s)	25, 705
FokI	GGATG	5 cut(s)	560, 679, 780, 977, 1772
FriOI	GRGCYC	5 cut(s)	624, 742, 775, 1065, 1571
FspBI	CTAG	3 cut(s)	192, 779, 941
GsuI	CTGGAG	3 cut(s)	268, 908, 1731
HaeIII	GGCC	2 cut(s)	69, 905
HapII	CCGG	1 cut(s)	599
HgaI	GACGC	1 cut(s)	716
Hin1I	GRCGYC	1 cut(s)	727
Hin1II	CATG	8 cut(s)	241, 440, 802, 1028, 1106, 1169, 1585, 1796
HincII	GTYRAC	1 cut(s)	814
HindII	GTYRAC	1 cut(s)	814
HindIII	AAGCTT	2 cut(s)	198, 569
HinfI	GANTC	3 cut(s)	38, 1399, 1555
HpaI	GTTAAC	1 cut(s)	814
HpaII	CCGG	1 cut(s)	599
HphI	GGTGA	1 cut(s)	613
Hpy166II	GTNNAC	3 cut(s)	26, 706, 814
Hpy188I	TCNGA	9 cut(s)	697, 717, 1182, 1276, 1319, 1483, 1563, 1573, 1731
Hpy188III	TCNNGA	5 cut(s)	285, 757, 1166, 1424, 1748
Hpy8I	GTNNAC	3 cut(s)	26, 706, 814
HpyAV	CCTTC	5 cut(s)	44, 598, 889, 1169, 1558
HpyCH4III	ACNGT	7 cut(s)	181, 538, 1048, 1225, 1300, 1468, 1634
HpyF10VI	GCNNNNNNNGC	8 cut(s)	197, 469, 679, 779, 788, 946, 1246, 1541
HpyF3I	CTNAG	7 cut(s)	30, 512, 714, 1251, 1455, 1495, 1620
Hsp92I	GRCGYC	1 cut(s)	727
Hsp92II	CATG	8 cut(s)	241, 440, 802, 1028, 1106, 1169, 1585, 1796
KflI	GGGWCCC	1 cut(s)	1290
KspAI	GTTAAC	1 cut(s)	814
Kzo9I	GATC	6 cut(s)	321, 577, 581, 633, 922, 1204
LguI	GCTCTTC	1 cut(s)	612
LmnI	GCTCC	4 cut(s)	937, 946, 1060, 1114
Lsp1109I	GCAGC	8 cut(s)	121, 319, 633, 654, 682, 1031, 1042, 1489
LweI	GCATC	1 cut(s)	1750
MaeI	CTAG	3 cut(s)	192, 779, 941
MaeIII	GTNAC	4 cut(s)	394, 532, 1384, 1634
MalI	GATC	6 cut(s)	323, 579, 583, 635, 924, 1206
MboI	GATC	6 cut(s)	321, 577, 581, 633, 922, 1204
MfeI	CAATTG	1 cut(s)	205
MhlI	GDGCHC	5 cut(s)	624, 742, 775, 1065, 1571
MlsI	TGGCCA	1 cut(s)	69
MluNI	TGGCCA	1 cut(s)	69
Mox20I	TGGCCA	1 cut(s)	69
MroXI	GAANNNNTTC	2 cut(s)	168, 1173
MscI	TGGCCA	1 cut(s)	69
MseI	TTAA	8 cut(s)	156, 363, 525, 813, 1010, 1394, 1685, 1709
MslI	CAYNNNNRTG	2 cut(s)	599, 1107
Msp20I	TGGCCA	1 cut(s)	69
MspA1I	CMGCKG	3 cut(s)	332, 667, 1776
MspI	CCGG	1 cut(s)	599
MspR9I	CCNGG	2 cut(s)	614, 907
MunI	CAATTG	1 cut(s)	205
MvaI	CCWGG	2 cut(s)	614, 907
MwoI	GCNNNNNNNGC	8 cut(s)	197, 469, 679, 779, 788, 946, 1246, 1541
NdeI	CATATG	1 cut(s)	1529
NdeII	GATC	6 cut(s)	321, 577, 581, 633, 922, 1204
NlaIII	CATG	8 cut(s)	241, 440, 802, 1028, 1106, 1169, 1585, 1796
NlaIV	GGNNCC	4 cut(s)	248, 1116, 1291, 1292
NmuCI	GTSAC	2 cut(s)	1384, 1634
NspI	RCATGY	3 cut(s)	241, 802, 1796
PaeI	GCATGC	1 cut(s)	241
PagI	TCATGA	1 cut(s)	1165
PciI	ACATGT	1 cut(s)	798
PciSI	GCTCTTC	1 cut(s)	612
PdmI	GAANNNNTTC	2 cut(s)	168, 1173
PfeI	GAWTC	3 cut(s)	38, 1399, 1555
PflFI	GACNNNGTC	1 cut(s)	1490
PpuMI	RGGWCCY	1 cut(s)	1290
PscI	ACATGT	1 cut(s)	798
PshAI	GACNNNNGTC	1 cut(s)	405
Psp124BI	GAGCTC	2 cut(s)	742, 1065
Psp5II	RGGWCCY	1 cut(s)	1290
Psp6I	CCWGG	2 cut(s)	612, 905
PspGI	CCWGG	2 cut(s)	612, 905
PspN4I	GGNNCC	4 cut(s)	248, 1116, 1291, 1292
PspPI	GGNCC	1 cut(s)	1290
PspPPI	RGGWCCY	1 cut(s)	1290
PstI	CTGCAG	1 cut(s)	672
PsyI	GACNNNGTC	1 cut(s)	1490
PvuII	CAGCTG	1 cut(s)	667
RsaI	GTAC	4 cut(s)	35, 368, 1256, 1475
RsaNI	GTAC	4 cut(s)	34, 367, 1255, 1474
RseI	CAYNNNNRTG	2 cut(s)	599, 1107
SacI	GAGCTC	2 cut(s)	742, 1065
SapI	GCTCTTC	1 cut(s)	612
SaqAI	TTAA	8 cut(s)	156, 363, 525, 813, 1010, 1394, 1685, 1709
Sau3AI	GATC	6 cut(s)	321, 577, 581, 633, 922, 1204
Sau96I	GGNCC	1 cut(s)	1290
ScaI	AGTACT	1 cut(s)	1256
ScrFI	CCNGG	2 cut(s)	614, 907
SduI	GDGCHC	5 cut(s)	624, 742, 775, 1065, 1571
SfaNI	GCATC	1 cut(s)	1750
SfcI	CTRYAG	1 cut(s)	668
SinI	GGWCC	1 cut(s)	1290
SmiMI	CAYNNNNRTG	2 cut(s)	599, 1107
SmlI	CTYRAG	2 cut(s)	674, 1718
SmoI	CTYRAG	2 cut(s)	674, 1718
SphI	GCATGC	1 cut(s)	241
SsiI	CCGC	5 cut(s)	330, 541, 1247, 1771, 1774
SspI	AATATT	1 cut(s)	1172
SspMI	CTAG	3 cut(s)	192, 779, 941
SstI	GAGCTC	2 cut(s)	742, 1065
StyD4I	CCNGG	2 cut(s)	612, 905
TaaI	ACNGT	7 cut(s)	181, 538, 1048, 1225, 1300, 1468, 1634
TaqI	TCGA	2 cut(s)	499, 580
TatI	WGTACW	2 cut(s)	1254, 1473
TauI	GCSGC	2 cut(s)	544, 1776
TfiI	GAWTC	3 cut(s)	38, 1399, 1555
Tru1I	TTAA	8 cut(s)	156, 363, 525, 813, 1010, 1394, 1685, 1709
Tru9I	TTAA	8 cut(s)	156, 363, 525, 813, 1010, 1394, 1685, 1709
TscAI	CASTG	6 cut(s)	520, 631, 862, 1051, 1230, 1471
TseFI	GTSAC	2 cut(s)	1384, 1634
TseI	GCWGC	8 cut(s)	109, 332, 646, 667, 670, 1019, 1030, 1502
Tsp45I	GTSAC	2 cut(s)	1384, 1634
TspGWI	ACGGA	1 cut(s)	259
TspRI	CASTG	6 cut(s)	520, 631, 862, 1051, 1230, 1471
Tth111I	GACNNNGTC	1 cut(s)	1490
VpaK11BI	GGWCC	1 cut(s)	1290
XagI	CCTNNNNNAGG	1 cut(s)	910
XapI	RAATTY	1 cut(s)	521
XceI	RCATGY	3 cut(s)	241, 802, 1796
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1083
XmiI	GTMKAC	2 cut(s)	25, 705
XmnI	GAANNNNTTC	2 cut(s)	168, 1173
XspI	CTAG	3 cut(s)	192, 779, 941
ZrmI	AGTACT	1 cut(s)	1256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.