Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005969.1

Orthogroup ID

OG0016959

Physical Location & Seq

Genomic Coordinates Forward (+)

44844 .. 47233

Gene Structure 2390 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0005969.1_g000017.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1194 bp

atgttttggactttggaagctaatgtactcgcccccacagatcttacattggaggagaggcgagctgatttgatccattctgctgcaaccatcttggagaagaacaatttgatcaagcatgacacaaaaagtggatatttccaggtcacagacttgggtcgcattgctagccattgctacataactcatgggacaatatcgacatataatgagcatttgaagccaactatgggggatattgaactttatcaattgttctccttcagtgaagagttcaagtatgtcactgtgcggccggatgaaaatatggaacttgtaaaactatcggaatgtgcccccattccggtcaaggagagcctgaaagatcccagttgcaagattaatgttttgctgcaagcatatattgcacagctgaaatttgaagggttttcattgacacaagatatgttggatttgtttgcttttcttagcttttattcatttgtctctttgatttctcttggtcagagtgctgggcgtcttgtacgagctcttttcgagatcgttttgaaacgaggatgggctcaacttgcagacaaggctttgaacttgtgtaaaatggttagcaagaggatgtggagtgtccaaatacttcttcgccaattccatggaataacaaaagatatcttggtgaagttggagaagaataatttggcctggtacctcattgtacctgaaaagtatcctccaccgacagagttattggacctgcaaccacttcctgtgaccacattaaggaatccgctatatgaagctctgtatcaagatttcaagcagtttaataccgtccagactcaggtcttcactgttttgtataattctgatggcaatgtcttagttgctgcaccaacagggagtgggaagaccatatgtgcagagtttgctgtattgagggatcatcagaaggcctctgaaagtggcattgtgtatattgcccctgttgaaggtcttgccaaggaatggcacactgactggcaaaagaagtttggaatgggaccaaattgtcttaatctgcgtgttgttgaattaacaggggaaagagccacagatttaaagctacctgaaaaaggtcagattatcatcagactccagagaaatgggatgctttatctcgcggatggaaacagaggaagcatgtacaacaacgacacttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000354 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009506 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034622 GO:0034641 GO:0042623 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0046540 GO:0055044 GO:0065003 GO:0070013 GO:0070035 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:0140098 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C Sec63

Protein Analysis

397

Amino Acids

45.07

Weight (kDa)

6.47

Isoelectric Point (pI)

29.47

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016959)

Species	Orthologous Gene IDs
pyrus_communis	pycom04g21220 pycom12g23610
rosa_chinensis	RchiOBHm_Chr3g0448021
rosa_multiflora	Rmu_sc0005582.1_g000010 Rmu_sc0005969.1_g000017
rosa_rugosa	Rorug02G0607800
rosa_samantha	Rh3AG006900 Rh3AG007000 Rh3BG006600 Rh3BG006700 Rh3CG006800 Rh3CG006900 Rh3DG007200 Rh3DG007300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1041
Acc36I	ACCTGC	1 cut(s)	756
Acc65I	GGTACC	1 cut(s)	699
AccB1I	GGYRCC	1 cut(s)	699
AccB7I	CCANNNNNTGG	1 cut(s)	999
AccII	CGCG	1 cut(s)	1154
AciI	CCGC	3 cut(s)	292, 782, 1154
AclWI	GGATC	3 cut(s)	67, 359, 942
AcoI	YGGCCR	1 cut(s)	293
AcsI	RAATTY	1 cut(s)	416
AcuI	CTGAAG	1 cut(s)	247
AcyI	GRCGYC	1 cut(s)	517
AfaI	GTAC	5 cut(s)	27, 525, 701, 711, 1178
AfiI	CCNNNNNNNGG	7 cut(s)	230, 343, 774, 835, 983, 999, 1106
AgsI	TTSAA	9 cut(s)	220, 242, 277, 422, 550, 586, 811, 983, 1064
AjnI	CCWGG	2 cut(s)	141, 695
AjuI	GAANNNNNNNTTGG	4 cut(s)	618, 650, 674, 706
AluBI	AGCT	7 cut(s)	20, 65, 412, 471, 530, 794, 1096
AluI	AGCT	7 cut(s)	20, 65, 412, 471, 530, 794, 1096
Alw21I	GWGCWC	1 cut(s)	532
Alw26I	GTCTC	1 cut(s)	490
AlwI	GGATC	3 cut(s)	67, 359, 942
AoxI	GGCC	3 cut(s)	293, 693, 945
ApeKI	GCWGC	3 cut(s)	83, 391, 881
ApoI	RAATTY	1 cut(s)	416
AseI	ATTAAT	1 cut(s)	381
Asp718I	GGTACC	1 cut(s)	699
AspS9I	GGNCC	2 cut(s)	745, 1034
AsuHPI	GGTGA	1 cut(s)	682
AsuNHI	GCTAGC	1 cut(s)	167
AvaII	GGWCC	2 cut(s)	745, 1034
BaeGI	GKGCMC	1 cut(s)	337
BanI	GGYRCC	1 cut(s)	699
BanII	GRGCYC	2 cut(s)	532, 565
BarI	GAAGNNNNNNTAC	2 cut(s)	9, 41
BbsI	GAAGAC	2 cut(s)	832, 908
Bbv12I	GWGCWC	1 cut(s)	532
BbvI	GCAGC	3 cut(s)	70, 378, 868
BccI	CCATC	4 cut(s)	98, 552, 857, 1151
BciT130I	CCWGG	2 cut(s)	143, 697
BciVI	GTATCC	1 cut(s)	732
BclI	TGATCA	1 cut(s)	111
BcoDI	GTCTC	1 cut(s)	490
BfaI	CTAG	1 cut(s)	168
BfuAI	ACCTGC	1 cut(s)	756
BfuI	GTATCC	1 cut(s)	732
BglII	AGATCT	1 cut(s)	40
BisI	GCNGC	4 cut(s)	84, 293, 392, 882
BlsI	GCNGC	4 cut(s)	85, 294, 393, 883
Bme1390I	CCNGG	2 cut(s)	143, 697
Bme18I	GGWCC	2 cut(s)	745, 1034
BmgT120I	GGNCC	2 cut(s)	745, 1034
BmiI	GGNNCC	2 cut(s)	701, 1035
BmrFI	CCNGG	2 cut(s)	143, 697
BmrI	ACTGGG	1 cut(s)	363
BmsI	GCATC	1 cut(s)	1131
BmtI	GCTAGC	1 cut(s)	171
BmuI	ACTGGG	1 cut(s)	363
BoxI	GACNNNNGTC	2 cut(s)	156, 836
BpiI	GAAGAC	2 cut(s)	832, 908
BpmI	CTGGAG	1 cut(s)	1112
BsaBI	GATNNNNATC	1 cut(s)	1118
BsaHI	GRCGYC	1 cut(s)	517
BsaJI	CCNNGG	2 cut(s)	646, 993
BsaWI	WCCGGW	1 cut(s)	343
Bsc4I	CCNNNNNNNGG	7 cut(s)	230, 343, 774, 835, 983, 999, 1106
Bse1I	ACTGG	2 cut(s)	369, 1016
Bse3DI	GCAATG	3 cut(s)	162, 172, 874
Bse8I	GATNNNNATC	1 cut(s)	1118
BseBI	CCWGG	2 cut(s)	143, 697
BseDI	CCNNGG	2 cut(s)	646, 993
BseGI	GGATG	5 cut(s)	304, 563, 618, 1146, 1162
BseJI	GATNNNNATC	1 cut(s)	1118
BseLI	CCNNNNNNNGG	7 cut(s)	230, 343, 774, 835, 983, 999, 1106
BseMI	GCAATG	3 cut(s)	162, 172, 874
BseMII	CTCAG	1 cut(s)	848
BseNI	ACTGG	2 cut(s)	369, 1016
BseRI	GAGGAG	1 cut(s)	68
BseSI	GKGCMC	1 cut(s)	337
BseX3I	CGGCCG	1 cut(s)	293
BseXI	GCAGC	3 cut(s)	70, 378, 868
BseYI	CCCAGC	1 cut(s)	512
BsgI	GTGCAG	2 cut(s)	867, 933
Bsh1236I	CGCG	1 cut(s)	1154
Bsh1285I	CGRYCG	1 cut(s)	296
BshFI	GGCC	3 cut(s)	295, 695, 947
BshNI	GGYRCC	1 cut(s)	699
BsiEI	CGRYCG	1 cut(s)	296
BsiHKAI	GWGCWC	1 cut(s)	532
BsiSI	CCGG	2 cut(s)	296, 344
BslFI	GGGAC	2 cut(s)	205, 1047
BslI	CCNNNNNNNGG	7 cut(s)	230, 343, 774, 835, 983, 999, 1106
BsmAI	GTCTC	1 cut(s)	490
BsmFI	GGGAC	2 cut(s)	205, 1047
BsnI	GGCC	3 cut(s)	295, 695, 947
Bsp1286I	GDGCHC	3 cut(s)	337, 532, 565
Bsp1407I	TGTACA	1 cut(s)	1176
Bsp143I	GATC	6 cut(s)	40, 72, 111, 364, 540, 934
Bsp19I	CCATGG	1 cut(s)	646
BspACI	CCGC	3 cut(s)	292, 782, 1154
BspANI	GGCC	3 cut(s)	295, 695, 947
BspCNI	CTCAG	1 cut(s)	847
BspFNI	CGCG	1 cut(s)	1154
BspLI	GGNNCC	2 cut(s)	701, 1035
BspMI	ACCTGC	1 cut(s)	756
BspOI	GCTAGC	1 cut(s)	171
BspPI	GGATC	3 cut(s)	67, 359, 942
BspT107I	GGYRCC	1 cut(s)	699
BsrDI	GCAATG	3 cut(s)	162, 172, 874
BsrGI	TGTACA	1 cut(s)	1176
BsrI	ACTGG	2 cut(s)	369, 1016
BssECI	CCNNGG	2 cut(s)	646, 993
BssMI	GATC	6 cut(s)	40, 72, 111, 364, 540, 934
BssNI	GRCGYC	1 cut(s)	517
BssT1I	CCWWGG	2 cut(s)	646, 993
Bst2UI	CCWGG	2 cut(s)	143, 697
Bst4CI	ACNGT	3 cut(s)	289, 826, 847
Bst6I	CTCTTC	1 cut(s)	264
BstACI	GRCGYC	1 cut(s)	517
BstAPI	GCANNNNNTGC	2 cut(s)	404, 920
BstAUI	TGTACA	1 cut(s)	1176
BstC8I	GCNNGC	3 cut(s)	63, 169, 396
BstDEI	CTNAG	3 cut(s)	467, 834, 874
BstDSI	CCRYGG	1 cut(s)	646
BstENI	CCTNNNNNAGG	2 cut(s)	981, 1104
BstF5I	GGATG	5 cut(s)	304, 563, 618, 1146, 1162
BstFNI	CGCG	1 cut(s)	1154
BstKTI	GATC	6 cut(s)	43, 75, 114, 367, 543, 937
BstMAI	GTCTC	1 cut(s)	490
BstMBI	GATC	6 cut(s)	40, 72, 111, 364, 540, 934
BstMCI	CGRYCG	1 cut(s)	296
BstMWI	GCNNNNNNNGC	6 cut(s)	168, 220, 404, 569, 578, 920
BstNI	CCWGG	2 cut(s)	143, 697
BstNSI	RCATGY	1 cut(s)	1177
BstPAI	GACNNNNGTC	2 cut(s)	156, 836
BstSCI	CCNGG	2 cut(s)	141, 695
BstSLI	GKGCMC	1 cut(s)	337
BstUI	CGCG	1 cut(s)	1154
BstV1I	GCAGC	3 cut(s)	70, 378, 868
BstV2I	GAAGAC	2 cut(s)	832, 908
BstX2I	RGATCY	2 cut(s)	40, 364
BstXI	CCANNNNNNTGG	2 cut(s)	647, 1136
BstYI	RGATCY	2 cut(s)	40, 364
BstZI	CGGCCG	1 cut(s)	293
BsuI	GTATCC	1 cut(s)	732
BsuRI	GGCC	3 cut(s)	295, 695, 947
BtgI	CCRYGG	1 cut(s)	646
BtsCI	GGATG	5 cut(s)	304, 563, 618, 1146, 1162
BtsIMutI	CAGTG	4 cut(s)	271, 285, 843, 1005
BveI	ACCTGC	1 cut(s)	756
Cac8I	GCNNGC	3 cut(s)	63, 169, 396
Cfr13I	GGNCC	2 cut(s)	745, 1034
CseI	GACGC	1 cut(s)	506
Csp6I	GTAC	5 cut(s)	26, 524, 700, 710, 1177
CviAII	CATG	4 cut(s)	119, 188, 647, 1174
CviQI	GTAC	5 cut(s)	26, 524, 700, 710, 1177
DdeI	CTNAG	3 cut(s)	467, 834, 874
DpnI	GATC	6 cut(s)	42, 74, 113, 366, 542, 936
DpnII	GATC	6 cut(s)	40, 72, 111, 364, 540, 934
DraI	TTTAAA	1 cut(s)	1092
DrdI	GACNNNNNNGTC	1 cut(s)	1041
DseDI	GACNNNNNNGTC	1 cut(s)	1041
EaeI	YGGCCR	1 cut(s)	293
EagI	CGGCCG	1 cut(s)	293
Eam1104I	CTCTTC	1 cut(s)	264
EarI	CTCTTC	1 cut(s)	264
Ecl136II	GAGCTC	1 cut(s)	530
EclXI	CGGCCG	1 cut(s)	293
Eco130I	CCWWGG	2 cut(s)	646, 993
Eco147I	AGGCCT	1 cut(s)	947
Eco24I	GRGCYC	2 cut(s)	532, 565
Eco32I	GATATC	1 cut(s)	664
Eco47I	GGWCC	2 cut(s)	745, 1034
Eco52I	CGGCCG	1 cut(s)	293
Eco53kI	GAGCTC	1 cut(s)	530
Eco57I	CTGAAG	1 cut(s)	247
EcoICRI	GAGCTC	1 cut(s)	530
EcoNI	CCTNNNNNAGG	2 cut(s)	981, 1104
EcoRII	CCWGG	2 cut(s)	141, 695
EcoRV	GATATC	1 cut(s)	664
EcoT14I	CCWWGG	2 cut(s)	646, 993
EcoT38I	GRGCYC	2 cut(s)	532, 565
ErhI	CCWWGG	2 cut(s)	646, 993
FaeI	CATG	4 cut(s)	122, 191, 650, 1177
FaqI	GGGAC	2 cut(s)	205, 1047
FatI	CATG	4 cut(s)	118, 187, 646, 1173
FauNDI	CATATG	1 cut(s)	908
FbaI	TGATCA	1 cut(s)	111
Fnu4HI	GCNGC	4 cut(s)	84, 293, 392, 882
FokI	GGATG	5 cut(s)	311, 570, 625, 1153, 1169
FriOI	GRGCYC	2 cut(s)	532, 565
Fsp4HI	GCNGC	4 cut(s)	84, 293, 392, 882
FspBI	CTAG	1 cut(s)	168
GluI	GCNGC	4 cut(s)	84, 293, 392, 882
GsaI	CCCAGC	1 cut(s)	516
GsuI	CTGGAG	1 cut(s)	1112
HaeIII	GGCC	3 cut(s)	295, 695, 947
HapII	CCGG	2 cut(s)	296, 344
HgaI	GACGC	1 cut(s)	506
Hin1I	GRCGYC	1 cut(s)	517
Hin1II	CATG	4 cut(s)	122, 191, 650, 1177
HinfI	GANTC	3 cut(s)	778, 832, 1125
HpaII	CCGG	2 cut(s)	296, 344
HphI	GGTGA	1 cut(s)	682
Hpy188I	TCNGA	7 cut(s)	328, 507, 862, 942, 952, 1113, 1124
Hpy188III	TCNNGA	4 cut(s)	538, 803, 829, 1129
HpyAV	CCTTC	4 cut(s)	271, 416, 937, 977
HpyCH4III	ACNGT	3 cut(s)	289, 826, 847
HpyCH4V	TGCA	8 cut(s)	86, 375, 394, 407, 572, 751, 884, 914
HpyF10VI	GCNNNNNNNGC	6 cut(s)	168, 220, 404, 569, 578, 920
HpyF3I	CTNAG	3 cut(s)	467, 834, 874
Hsp92I	GRCGYC	1 cut(s)	517
Hsp92II	CATG	4 cut(s)	122, 191, 650, 1177
KpnI	GGTACC	1 cut(s)	703
Ksp22I	TGATCA	1 cut(s)	111
Kzo9I	GATC	6 cut(s)	40, 72, 111, 364, 540, 934
Lsp1109I	GCAGC	3 cut(s)	70, 378, 868
LweI	GCATC	1 cut(s)	1131
MaeI	CTAG	1 cut(s)	168
MaeIII	GTNAC	3 cut(s)	145, 283, 763
MalI	GATC	6 cut(s)	42, 74, 113, 366, 542, 936
MboI	GATC	6 cut(s)	40, 72, 111, 364, 540, 934
MboII	GAAGA	6 cut(s)	112, 281, 626, 694, 832, 913
MfeI	CAATTG	1 cut(s)	251
MflI	RGATCY	2 cut(s)	40, 364
MhlI	GDGCHC	3 cut(s)	337, 532, 565
MluCI	AATT	8 cut(s)	106, 251, 416, 641, 688, 856, 1039, 1064
MlyI	GAGTC	2 cut(s)	826, 1119
MmeI	TCCRAC	2 cut(s)	429, 657
MnlI	CCTC	9 cut(s)	46, 51, 548, 603, 713, 735, 924, 958, 1160
MseI	TTAA	6 cut(s)	381, 773, 819, 1047, 1067, 1091
MspA1I	CMGCKG	1 cut(s)	412
MspI	CCGG	2 cut(s)	296, 344
MspR9I	CCNGG	2 cut(s)	143, 697
MunI	CAATTG	1 cut(s)	251
MvaI	CCWGG	2 cut(s)	143, 697
MvnI	CGCG	1 cut(s)	1154
MwoI	GCNNNNNNNGC	6 cut(s)	168, 220, 404, 569, 578, 920
NcoI	CCATGG	1 cut(s)	646
NdeI	CATATG	1 cut(s)	908
NdeII	GATC	6 cut(s)	40, 72, 111, 364, 540, 934
NheI	GCTAGC	1 cut(s)	167
NlaIII	CATG	4 cut(s)	122, 191, 650, 1177
NlaIV	GGNNCC	2 cut(s)	701, 1035
NmuCI	GTSAC	3 cut(s)	145, 283, 763
NspI	RCATGY	1 cut(s)	1177
PceI	AGGCCT	1 cut(s)	947
PfeI	GAWTC	1 cut(s)	778
PflMI	CCANNNNNTGG	1 cut(s)	999
PkrI	GCNGC	4 cut(s)	85, 294, 393, 883
PleI	GAGTC	2 cut(s)	826, 1119
PpsI	GAGTC	2 cut(s)	826, 1119
PshAI	GACNNNNGTC	2 cut(s)	156, 836
PshBI	ATTAAT	1 cut(s)	381
Psp124BI	GAGCTC	1 cut(s)	532
Psp6I	CCWGG	2 cut(s)	141, 695
PspFI	CCCAGC	1 cut(s)	512
PspGI	CCWGG	2 cut(s)	141, 695
PspN4I	GGNNCC	2 cut(s)	701, 1035
PspPI	GGNCC	2 cut(s)	745, 1034
PsuI	RGATCY	2 cut(s)	40, 364
PvuII	CAGCTG	1 cut(s)	412
RsaI	GTAC	5 cut(s)	27, 525, 701, 711, 1178
RsaNI	GTAC	5 cut(s)	26, 524, 700, 710, 1177
SacI	GAGCTC	1 cut(s)	532
SaqAI	TTAA	6 cut(s)	381, 773, 819, 1047, 1067, 1091
SatI	GCNGC	4 cut(s)	84, 293, 392, 882
Sau3AI	GATC	6 cut(s)	40, 72, 111, 364, 540, 934
Sau96I	GGNCC	2 cut(s)	745, 1034
SchI	GAGTC	2 cut(s)	826, 1119
ScrFI	CCNGG	2 cut(s)	143, 697
SduI	GDGCHC	3 cut(s)	337, 532, 565
SfaNI	GCATC	1 cut(s)	1131
SinI	GGWCC	2 cut(s)	745, 1034
Sse9I	AATT	8 cut(s)	106, 251, 416, 641, 688, 856, 1039, 1064
SseBI	AGGCCT	1 cut(s)	947
SsiI	CCGC	3 cut(s)	292, 782, 1154
SspMI	CTAG	1 cut(s)	168
SstI	GAGCTC	1 cut(s)	532
StuI	AGGCCT	1 cut(s)	947
StyD4I	CCNGG	2 cut(s)	141, 695
StyI	CCWWGG	2 cut(s)	646, 993
TaaI	ACNGT	3 cut(s)	289, 826, 847
TaqI	TCGA	2 cut(s)	200, 537
TasI	AATT	8 cut(s)	106, 251, 416, 641, 688, 856, 1039, 1064
TatI	WGTACW	2 cut(s)	25, 1176
TauI	GCSGC	1 cut(s)	295
TfiI	GAWTC	1 cut(s)	778
Tru1I	TTAA	6 cut(s)	381, 773, 819, 1047, 1067, 1091
Tru9I	TTAA	6 cut(s)	381, 773, 819, 1047, 1067, 1091
TscAI	CASTG	4 cut(s)	271, 292, 850, 1012
TseFI	GTSAC	3 cut(s)	145, 283, 763
TseI	GCWGC	3 cut(s)	83, 391, 881
Tsp45I	GTSAC	3 cut(s)	145, 283, 763
TspDTI	ATGAA	4 cut(s)	315, 420, 468, 804
TspRI	CASTG	4 cut(s)	271, 292, 850, 1012
Van91I	CCANNNNNTGG	1 cut(s)	999
VpaK11BI	GGWCC	2 cut(s)	745, 1034
VspI	ATTAAT	1 cut(s)	381
XagI	CCTNNNNNAGG	2 cut(s)	981, 1104
XapI	RAATTY	1 cut(s)	416
XceI	RCATGY	1 cut(s)	1177
XspI	CTAG	1 cut(s)	168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.