pycom05g00500

Guanosine nucleotide diphosphate dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0020392
Physical Location & Seq
Reverse (-)
652684 .. 654014
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00500.1

Sequence Viewer

Length: 582 bp
ATGGAGGCCCTTAAATCTCCACTCATGGGTTTGTTTGAAAAGCGCCATGCACGCAAGTTCATCCTATATGTTCAAGATTATAGTGAAACTAATCCTAAAACACATGAGGGAATGGACTTAACAAGAGTGACAACTAGAGATTTCATCTTATGCCCTACAAGTATGCATACGTTTGTTTTTATTTATAGGTCTGCAAGGGACACAATATCTTCTCTGTTCTTCTGTATCTTGCTTGAAGGATATTATCTTTTCTGTTGCTCCTACTCTCACAATGTTGCTCCAAGGGGAAACTTTATTGCATTTGTATCAACAGACGCACAGACTGATCATCCCGAGACTGAGCTAAAGCCAGGGATTGACCTTTTAGGGCCTATTGACGAGATATTTTTTAAGACTTATGACAGATGTGAGCTTGTTAATGAACCCAATTTGGACAATTGCTTTATATCAACCAGTTATGATGCCTCCACACACTTTGAGTGCACTGTCTTGGATGTACTCAACATGTACACCTTAATAACAGGGAAGGTAAGAAATACAATTTTATGCATTGCCCAATTAGTTTGGTTCTGTTGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0003674 GO:0005092 GO:0005093 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007275 GO:0007399 GO:0008150 GO:0008361 GO:0009506 GO:0009987 GO:0010035 GO:0010038 GO:0010720 GO:0010721 GO:0010769 GO:0010770 GO:0010771 GO:0010975 GO:0010976 GO:0010977 GO:0016043 GO:0017016 GO:0017137 GO:0019899 GO:0022008 GO:0022603 GO:0022604 GO:0023052 GO:0030054 GO:0030154 GO:0030234 GO:0030307 GO:0030424 GO:0030516 GO:0030695 GO:0031267 GO:0031344 GO:0031345 GO:0031346 GO:0032386 GO:0032387 GO:0032482 GO:0032501 GO:0032502 GO:0032535 GO:0032879 GO:0032880 GO:0032991 GO:0033157 GO:0035556 GO:0036477 GO:0040008 GO:0042221 GO:0042995 GO:0043005 GO:0043025 GO:0043209 GO:0044297 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0045596 GO:0045597 GO:0045664 GO:0045665 GO:0045666 GO:0045773 GO:0045927 GO:0048046 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050768 GO:0050769 GO:0050770 GO:0050771 GO:0050772 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051051 GO:0051093 GO:0051094 GO:0051128 GO:0051129 GO:0051130 GO:0051223 GO:0051224 GO:0051239 GO:0051240 GO:0051241 GO:0051592 GO:0051716 GO:0051960 GO:0051961 GO:0051962 GO:0055044 GO:0060284 GO:0060341 GO:0060589 GO:0061387 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0071840 GO:0090066 GO:0090087 GO:0090313 GO:0090315 GO:0090317 GO:0097458 GO:0098772 GO:0120025 GO:0120035 GO:1903533 GO:1903827 GO:1903828 GO:1904950 GO:1905475 GO:1905476 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GDI

Protein Analysis

194

Amino Acids

22.27

Weight (kDa)

5.29

Isoelectric Point (pI)

31.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDI PF00996 1 - 48 2.3e-14 GDP dissociation inhibitor
GDI PF00996 81 - 177 5.8e-34 GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020392)

Species Orthologous Gene IDs
malus_domestica MD04G1207800.v1.1 MD12G1166400.v1.1
pyrus_communis pycom05g00500 pycom06g10650 pycom16g17470
rosa_multiflora Rmu_co8392277.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 2 cut(s) 498, 509
AfiI CCNNNNNNNGG 1 cut(s) 26
AflIII ACRYGT 1 cut(s) 504
AgsI TTSAA 3 cut(s) 38, 74, 236
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 2 cut(s) 343, 412
AluI AGCT 2 cut(s) 343, 412
Alw21I GWGCWC 1 cut(s) 485
Alw26I GTCTC 1 cut(s) 329
Alw44I GTGCAC 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 2 cut(s) 6, 368
ApaLI GTGCAC 1 cut(s) 481
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 7, 368
AvaI CYCGRG 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 485
BciT130I CCWGG 1 cut(s) 351
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 1 cut(s) 135
BfoI RGCGCY 1 cut(s) 46
Bme1390I CCNGG 1 cut(s) 351
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 7, 368
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 1 cut(s) 451
BsaJI CCNNGG 2 cut(s) 281, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 1 cut(s) 549
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 2 cut(s) 281, 350
BseGI GGATG 3 cut(s) 60, 328, 499
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 1 cut(s) 453
BseSI GKGCMC 1 cut(s) 485
BshFI GGCC 2 cut(s) 8, 370
BsiHKAI GWGCWC 1 cut(s) 485
BsiHKCI CYCGRG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 2 cut(s) 8, 370
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 485
Bsp1407I TGTACA 1 cut(s) 507
Bsp143I GATC 1 cut(s) 325
BspANI GGCC 2 cut(s) 8, 370
BspCNI CTCAG 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 549
BsrGI TGTACA 1 cut(s) 507
BsrI ACTGG 1 cut(s) 453
BssECI CCNNGG 2 cut(s) 281, 350
BssMI GATC 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 487
BstAUI TGTACA 1 cut(s) 507
BstC8I GCNNGC 1 cut(s) 52
BstDEI CTNAG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 60, 328, 499
BstH2I RGCGCY 1 cut(s) 46
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 349
BstSLI GKGCMC 1 cut(s) 485
BsuRI GGCC 2 cut(s) 8, 370
BtsCI GGATG 3 cut(s) 60, 328, 499
BtsIMutI CAGTG 1 cut(s) 483
Cac8I GCNNGC 1 cut(s) 52
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 2 cut(s) 7, 368
CseI GACGC 1 cut(s) 323
Csp6I GTAC 2 cut(s) 497, 508
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 4 cut(s) 25, 47, 104, 505
CviJI RGCY 5 cut(s) 8, 343, 349, 370, 412
CviKI_1 RGCY 5 cut(s) 8, 343, 349, 370, 412
CviQI GTAC 2 cut(s) 497, 508
DdeI CTNAG 1 cut(s) 339
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 332
EcoO109I RGGNCCY 2 cut(s) 7, 368
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 281
EcoT22I ATGCAT 2 cut(s) 168, 551
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 4 cut(s) 28, 50, 107, 508
FaqI GGGAC 1 cut(s) 212
FatI CATG 4 cut(s) 24, 46, 103, 504
FbaI TGATCA 1 cut(s) 325
FokI GGATG 3 cut(s) 47, 315, 506
FspBI CTAG 1 cut(s) 135
GlaI GCGC 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 46
HaeIII GGCC 2 cut(s) 8, 370
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 45
Hin1II CATG 4 cut(s) 28, 50, 107, 508
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
Hpy166II GTNNAC 2 cut(s) 483, 510
Hpy188III TCNNGA 2 cut(s) 74, 332
Hpy8I GTNNAC 2 cut(s) 483, 510
HpyAV CCTTC 2 cut(s) 230, 520
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 6 cut(s) 50, 166, 194, 299, 483, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 1 cut(s) 170
Hsp92II CATG 4 cut(s) 28, 50, 107, 508
HspAI GCGC 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 2 cut(s) 263, 283
LpnPI CCDG 4 cut(s) 336, 363, 466, 507
LweI GCATC 1 cut(s) 451
MaeI CTAG 1 cut(s) 135
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 127
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 2 cut(s) 201, 211
MfeI CAATTG 1 cut(s) 436
MhlI GDGCHC 1 cut(s) 485
MluCI AATT 4 cut(s) 427, 436, 540, 557
MnlI CCTC 2 cut(s) 100, 475
Mph1103I ATGCAT 2 cut(s) 168, 551
MseI TTAA 5 cut(s) 12, 119, 390, 417, 515
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 436
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 51
NdeII GATC 1 cut(s) 325
NlaIII CATG 4 cut(s) 28, 50, 107, 508
NmuCI GTSAC 1 cut(s) 127
NsiI ATGCAT 2 cut(s) 168, 551
NspI RCATGY 1 cut(s) 508
PciI ACATGT 1 cut(s) 504
PscI ACATGT 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspPI GGNCC 2 cut(s) 7, 368
RsaI GTAC 2 cut(s) 498, 509
RsaNI GTAC 2 cut(s) 497, 508
SaqAI TTAA 5 cut(s) 12, 119, 390, 417, 515
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 2 cut(s) 7, 368
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 1 cut(s) 485
SetI ASST 7 cut(s) 173, 191, 345, 363, 414, 515, 531
SfaNI GCATC 1 cut(s) 451
Sse9I AATT 4 cut(s) 427, 436, 540, 557
SspMI CTAG 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 487
TaiI ACGT 1 cut(s) 173
TasI AATT 4 cut(s) 427, 436, 540, 557
TatI WGTACW 2 cut(s) 496, 507
Tru1I TTAA 5 cut(s) 12, 119, 390, 417, 515
Tru9I TTAA 5 cut(s) 12, 119, 390, 417, 515
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 49, 133, 435
TspRI CASTG 1 cut(s) 490
VneI GTGCAC 1 cut(s) 481
XceI RCATGY 1 cut(s) 508
XspI CTAG 1 cut(s) 135
Zsp2I ATGCAT 2 cut(s) 168, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.