pycom05g02490

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016756
Physical Location & Seq
Reverse (-)
2987213 .. 2987972
760 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGCCCTCCCTCACATCCCTCATTGTCAACCGTTTCAAGCTCAAACCCAGCGTAAAAACGTACAATTTAAGTGGCATGGGATGTAGTTTGCAAAGATGGAAGTCATTCACTCCCTTCGTACTCACCACGGAGCGAATGCTCGAGCCTATAAAGCATAATTTCAAGAGGAGGACGACAAGGGCAATAACGGCGTTGAGTGCATTGTCGGGGAGCAAGTCGAAACCGATAGTCCCGGATTTCACCAAGGCGTTTGATCACTTTTGTATTCACACGGGCGGGAAAGCGGTGATTGAGCAGGTCGAGCGGGTGTTGAGGTTGGGTGACGAGTTGACTGAGCCGGCTCGGATGACTTTGCACCGATTCGGGAACACATCGAGTAGTCTTGTGTTTTATGAGTTAGCGTATTTTGAGGCTAAGGGGAGAGTCAGGAAAGGAGATAGGGTGTGGATGTTGGCGTTTGGGACAGATTTCAAGGTTGGAAGTCTTGTCTGGAAGGCAGTTTCTGATTTAGGTCAAGGTGGTGATGATAATCCATGGAGTGACTGCAGTGATAGGTACCCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

189

Amino Acids

21.21

Weight (kDa)

9.74

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 2 - 29 6.1e-09 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 84 - 165 5.9e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 87 - 167 1.5e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016756)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12940
malus_domestica MD10G1036400.v1.1
prunus_persica Prupe.8G042200_v2.0.a1
pyrus_communis pycom05g02490 pycom10g02490
rosa_chinensis RchiOBHm_Chr6g0274011
rosa_laevigata RLG00000013584
rosa_multiflora Rmu_sc0002247.1_g000012
rosa_rugosa Rorug06G0078400
rosa_samantha Rh6AG193900 Rh6BG197500 Rh6CG197600 Rh6DG189300
rosa_wichuraiana Rw6G016910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 565
Acc36I ACCTGC 1 cut(s) 286
Acc65I GGTACC 1 cut(s) 555
AccB1I GGYRCC 1 cut(s) 555
AccBSI CCGCTC 1 cut(s) 304
AciI CCGC 3 cut(s) 276, 284, 304
AfaI GTAC 3 cut(s) 62, 120, 557
AgsI TTSAA 3 cut(s) 37, 163, 472
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 1 cut(s) 140
Asp700I GAANNNNTTC 1 cut(s) 104
Asp718I GGTACC 1 cut(s) 555
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 5 cut(s) 115, 232, 298, 332, 533
AvaI CYCGRG 1 cut(s) 140
BanI GGYRCC 1 cut(s) 555
BccI CCATC 1 cut(s) 90
BceAI ACGGC 1 cut(s) 204
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 233
BfmI CTRYAG 1 cut(s) 544
BfuAI ACCTGC 1 cut(s) 286
Bme1390I CCNGG 1 cut(s) 233
BmeT110I CYCGRG 1 cut(s) 140
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 1 cut(s) 233
Bpu10I CCTNAGC 1 cut(s) 414
BpuMI CCSGG 1 cut(s) 233
BsaBI GATNNNNATC 1 cut(s) 528
BsaJI CCNNGG 3 cut(s) 126, 243, 533
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bse118I RCCGGY 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 528
BseDI CCNNGG 3 cut(s) 126, 243, 533
BseGI GGATG 4 cut(s) 14, 86, 351, 453
BseJI GATNNNNATC 1 cut(s) 528
BseMII CTCAG 1 cut(s) 324
BseRI GAGGAG 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 555
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 2 cut(s) 233, 338
BslFI GGGAC 2 cut(s) 215, 475
BsmFI GGGAC 2 cut(s) 215, 475
BsmI GAATGC 1 cut(s) 141
BsoBI CYCGRG 1 cut(s) 140
Bsp143I GATC 1 cut(s) 253
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 3 cut(s) 276, 284, 304
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 557
BspMAI CTGCAG 1 cut(s) 548
BspMI ACCTGC 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 555
BsrBI CCGCTC 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 337
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 3 cut(s) 126, 243, 533
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 2 cut(s) 243, 533
Bst4CI ACNGT 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 2 cut(s) 333, 414
BstDSI CCRYGG 2 cut(s) 126, 533
BstF5I GGATG 4 cut(s) 14, 86, 351, 453
BstKTI GATC 1 cut(s) 256
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 4 cut(s) 151, 188, 197, 301
BstSCI CCNGG 1 cut(s) 231
BstSFI CTRYAG 1 cut(s) 544
BtgI CCRYGG 2 cut(s) 126, 533
BtsCI GGATG 4 cut(s) 14, 86, 351, 453
BtsI GCAGTG 1 cut(s) 553
BtsIMutI CAGTG 1 cut(s) 553
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 1 cut(s) 339
CaiI CAGNNNCTG 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 337
Csp6I GTAC 3 cut(s) 61, 119, 556
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 2 cut(s) 76, 534
CviJI RGCY 5 cut(s) 40, 145, 337, 341, 413
CviKI_1 RGCY 5 cut(s) 40, 145, 337, 341, 413
CviQI GTAC 3 cut(s) 61, 119, 556
DdeI CTNAG 2 cut(s) 333, 414
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 243, 533
Eco88I CYCGRG 1 cut(s) 140
EcoT14I CCWWGG 2 cut(s) 243, 533
ErhI CCWWGG 2 cut(s) 243, 533
FaeI CATG 2 cut(s) 79, 537
FaiI YATR 6 cut(s) 77, 149, 156, 393, 535, 565
FaqI GGGAC 2 cut(s) 215, 475
FatI CATG 2 cut(s) 75, 533
FauI CCCGC 2 cut(s) 269, 297
FbaI TGATCA 1 cut(s) 253
FokI GGATG 3 cut(s) 93, 358, 460
GsaI CCCAGC 1 cut(s) 51
HapII CCGG 2 cut(s) 233, 338
Hin1II CATG 2 cut(s) 79, 537
HincII GTYRAC 2 cut(s) 28, 330
HindII GTYRAC 2 cut(s) 28, 330
HinfI GANTC 2 cut(s) 360, 423
HpaII CCGG 2 cut(s) 233, 338
HphI GGTGA 5 cut(s) 115, 232, 298, 332, 533
Hpy166II GTNNAC 2 cut(s) 28, 330
Hpy188I TCNGA 2 cut(s) 345, 505
Hpy188III TCNNGA 4 cut(s) 163, 364, 427, 490
Hpy8I GTNNAC 2 cut(s) 28, 330
HpyAV CCTTC 2 cut(s) 124, 487
HpyCH4III ACNGT 1 cut(s) 32
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 4 cut(s) 91, 200, 355, 546
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 188, 197, 301
HpyF3I CTNAG 2 cut(s) 333, 414
HpySE526I ACGT 1 cut(s) 59
Hsp92II CATG 2 cut(s) 79, 537
KpnI GGTACC 1 cut(s) 559
KroI GCCGGC 1 cut(s) 337
KroNI GCCGGC 1 cut(s) 339
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 1 cut(s) 253
LmnI GCTCC 2 cut(s) 130, 210
LpnPI CCDG 6 cut(s) 61, 246, 281, 351, 412, 475
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 2 cut(s) 320, 539
MalI GATC 1 cut(s) 255
MbiI CCGCTC 1 cut(s) 304
MboI GATC 1 cut(s) 253
MluCI AATT 2 cut(s) 64, 157
MlyI GAGTC 1 cut(s) 432
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 7 cut(s) 16, 20, 29, 159, 162, 306, 403
MroNI GCCGGC 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 104
MseI TTAA 1 cut(s) 68
MspI CCGG 2 cut(s) 233, 338
MspR9I CCNGG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 141
MwoI GCNNNNNNNGC 4 cut(s) 151, 188, 197, 301
NaeI GCCGGC 1 cut(s) 339
NciI CCSGG 1 cut(s) 233
NcoI CCATGG 1 cut(s) 533
NdeII GATC 1 cut(s) 253
NgoMIV GCCGGC 1 cut(s) 337
NlaIII CATG 2 cut(s) 79, 537
NlaIV GGNNCC 1 cut(s) 557
NmuCI GTSAC 2 cut(s) 320, 539
PaeR7I CTCGAG 1 cut(s) 140
PctI GAATGC 1 cut(s) 141
PdiI GCCGGC 1 cut(s) 339
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 360
PfoI TCCNGGA 1 cut(s) 231
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PsiI TTATAA 1 cut(s) 565
PspFI CCCAGC 1 cut(s) 47
PspN4I GGNNCC 1 cut(s) 557
PspXI VCTCGAGB 1 cut(s) 140
PstI CTGCAG 1 cut(s) 548
PstNI CAGNNNCTG 1 cut(s) 503
RsaI GTAC 3 cut(s) 62, 120, 557
RsaNI GTAC 3 cut(s) 61, 119, 556
SaqAI TTAA 1 cut(s) 68
Sau3AI GATC 1 cut(s) 253
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 1 cut(s) 233
SetI ASST 8 cut(s) 42, 62, 300, 317, 477, 514, 520, 557
SfcI CTRYAG 1 cut(s) 544
Sfr274I CTCGAG 1 cut(s) 140
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 2 cut(s) 64, 157
SsiI CCGC 3 cut(s) 276, 284, 304
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 2 cut(s) 243, 533
TaaI ACNGT 1 cut(s) 32
TaiI ACGT 1 cut(s) 62
TaqI TCGA 4 cut(s) 141, 218, 300, 374
TasI AATT 2 cut(s) 64, 157
TfiI GAWTC 1 cut(s) 360
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 1 cut(s) 553
TseFI GTSAC 2 cut(s) 320, 539
Tsp45I GTSAC 2 cut(s) 320, 539
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 553
XhoI CTCGAG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.