pycom05g03150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0020212
Physical Location & Seq
Reverse (-)
3655719 .. 3659333
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03150.2

Sequence Viewer

Length: 474 bp
ATGATCCTCCCTTGCCTTTACCAATTACCGCCTATAAATACAACACTCTCCTCCACTCTCTCCCTCGCTGAAAGAATCAACGCCGCCAGCCACCACCGCCTTAGCAAGCATTGCGCATCAAACTCAGAACACAGAAGATTTGAACTCTGCAATCGGTGGCACCTTGCAAATCTAAGTATGAGTTTGGCATGCCTTGTGTGCAGTGTAGAAAGTCCGTCACACTCTTTTAGGAGTTACTCTGTATCGAGCACAGAAAATGATGGAAGATGTTCTGCTATTGCCAACTGCTTAACACGGAGGCCATCTCTCCCATTGCCAAGACATTCCAACATTGCTTCTTCCAAAGTAACCCCGCAACCAACTCTTTCAAACAGCGGTTCCATACCAGGTACCCCAAGGCTAGTTCGAAGCCGGGCTGTGAGAAGGGATATCGTGAGGGACTGGAACTTTGACGAGGTTGTGATGGGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.47

Weight (kDa)

9.87

Isoelectric Point (pI)

65.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71865
malus_domestica MD05G1038600.v1.1 MD10G1044400.v1.1
prunus_persica Prupe.8G051200_v2.0.a1
pyrus_communis pycom05g03150 pycom10g03100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 115
Acc65I GGTACC 1 cut(s) 389
AccB1I GGYRCC 2 cut(s) 159, 389
AciI CCGC 5 cut(s) 29, 84, 97, 353, 375
AfaI GTAC 1 cut(s) 391
AgsI TTSAA 2 cut(s) 143, 369
AjnI CCWGG 1 cut(s) 385
Alw21I GWGCWC 1 cut(s) 251
AoxI GGCC 1 cut(s) 299
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
Asp700I GAANNNNTTC 1 cut(s) 268
Asp718I GGTACC 1 cut(s) 389
AspLEI GCGC 1 cut(s) 116
AsuC2I CCSGG 1 cut(s) 413
AsuII TTCGAA 1 cut(s) 406
BanI GGYRCC 2 cut(s) 159, 389
Bbv12I GWGCWC 1 cut(s) 251
BccI CCATC 3 cut(s) 254, 310, 457
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 413
BfaI CTAG 1 cut(s) 401
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 387, 413
BmiI GGNNCC 3 cut(s) 161, 379, 391
BmrFI CCNGG 2 cut(s) 387, 413
BmsI GCATC 1 cut(s) 125
BplI GAGNNNNNCTC 2 cut(s) 289, 321
Bpu10I CCTNAGC 1 cut(s) 101
Bpu14I TTCGAA 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 446
Bse3DI GCAATG 3 cut(s) 109, 311, 330
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 395
BseMI GCAATG 3 cut(s) 109, 311, 330
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 1 cut(s) 446
BseRI GAGGAG 1 cut(s) 40
BsgI GTGCAG 1 cut(s) 220
BshFI GGCC 1 cut(s) 301
BshNI GGYRCC 2 cut(s) 159, 389
BsiHKAI GWGCWC 1 cut(s) 251
BsiSI CCGG 1 cut(s) 412
BslFI GGGAC 1 cut(s) 452
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 1 cut(s) 301
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 5 cut(s) 29, 84, 97, 353, 375
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 1 cut(s) 137
BspLI GGNNCC 3 cut(s) 161, 379, 391
BspT104I TTCGAA 1 cut(s) 406
BspT107I GGYRCC 2 cut(s) 159, 389
BsrDI GCAATG 3 cut(s) 109, 311, 330
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 387
BstAPI GCANNNNNTGC 1 cut(s) 111
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 3 cut(s) 88, 107, 190
BstDEI CTNAG 3 cut(s) 101, 124, 173
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 3 cut(s) 96, 111, 198
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 1 cut(s) 192
BstSCI CCNGG 2 cut(s) 385, 411
BsuRI GGCC 1 cut(s) 301
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 1 cut(s) 208
Cac8I GCNNGC 3 cut(s) 88, 107, 190
CfoI GCGC 1 cut(s) 116
CsiI ACCWGGT 1 cut(s) 385
Csp6I GTAC 1 cut(s) 390
CviAII CATG 1 cut(s) 189
CviJI RGCY 5 cut(s) 90, 301, 400, 411, 416
CviKI_1 RGCY 5 cut(s) 90, 301, 400, 411, 416
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 3 cut(s) 101, 124, 173
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 395
Eco32I GATATC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 385
EcoRV GATATC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 395
FaeI CATG 1 cut(s) 192
FaiI YATR 4 cut(s) 35, 179, 190, 383
FaqI GGGAC 1 cut(s) 452
FatI CATG 1 cut(s) 188
FauI CCCGC 1 cut(s) 360
Fnu4HI GCNGC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 1 cut(s) 401
FspI TGCGCA 1 cut(s) 115
GlaI GCGC 1 cut(s) 115
GluI GCNGC 1 cut(s) 84
HaeIII GGCC 1 cut(s) 301
HapII CCGG 1 cut(s) 412
HhaI GCGC 1 cut(s) 116
Hin1II CATG 1 cut(s) 192
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HinfI GANTC 1 cut(s) 75
HpaII CCGG 1 cut(s) 412
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 1 cut(s) 433
HpyAV CCTTC 1 cut(s) 417
HpyCH4V TGCA 3 cut(s) 150, 167, 201
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 111, 198
HpyF3I CTNAG 3 cut(s) 101, 124, 173
Hsp92II CATG 1 cut(s) 192
HspAI GCGC 1 cut(s) 114
KpnI GGTACC 1 cut(s) 393
Kzo9I GATC 1 cut(s) 3
LpnPI CCDG 5 cut(s) 100, 372, 399, 425, 427
LweI GCATC 1 cut(s) 125
MabI ACCWGGT 1 cut(s) 385
MaeI CTAG 1 cut(s) 401
MaeIII GTNAC 3 cut(s) 216, 233, 346
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 3 cut(s) 147, 276, 330
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 6 cut(s) 17, 61, 74, 291, 429, 448
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 2 cut(s) 290, 472
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 1 cut(s) 412
MspR9I CCNGG 2 cut(s) 387, 413
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 3 cut(s) 96, 111, 198
NciI CCSGG 1 cut(s) 413
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 192
NlaIV GGNNCC 3 cut(s) 161, 379, 391
NmuCI GTSAC 1 cut(s) 216
NsbI TGCGCA 1 cut(s) 115
NspI RCATGY 1 cut(s) 192
NspV TTCGAA 1 cut(s) 406
PaeI GCATGC 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 75
PkrI GCNGC 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 161, 379, 391
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
SaqAI TTAA 2 cut(s) 290, 472
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 1 cut(s) 3
ScrFI CCNGG 2 cut(s) 387, 413
SduI GDGCHC 1 cut(s) 251
SetI ASST 3 cut(s) 165, 391, 459
SexAI ACCWGGT 1 cut(s) 385
SfaNI GCATC 1 cut(s) 125
SfuI TTCGAA 1 cut(s) 406
SphI GCATGC 1 cut(s) 192
Sse9I AATT 1 cut(s) 23
SsiI CCGC 5 cut(s) 29, 84, 97, 353, 375
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 2 cut(s) 385, 411
StyI CCWWGG 1 cut(s) 395
TaqI TCGA 2 cut(s) 245, 406
TasI AATT 1 cut(s) 23
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 2 cut(s) 290, 472
Tru9I TTAA 2 cut(s) 290, 472
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 216
TspGWI ACGGA 2 cut(s) 204, 310
TspRI CASTG 1 cut(s) 208
XceI RCATGY 1 cut(s) 192
XmnI GAANNNNTTC 1 cut(s) 268
XspI CTAG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.