pycom05g14960

D-amino acid dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010015
Physical Location & Seq
Reverse (-)
18534792 .. 18536431
1640 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ATGGCTGCAGCTTCGACTTTTCTCGCGCAACCAAAGGCCCACACAAACGATGCAGTCTCATCTTTCCGAAGTCACTTCAAGCCATCCAGCTTCGCCTCCCAGTCAACATCCTTTGGCTCCTCCAAATTCGCATCCAAATCGCTCTCCTTGCGCACCGCCGCAACCCGATCCGGCCGACCCGAACCCGCCACCTTGTCCCATTCGGAATCCCAGTCTGATCAAACATTCGATGTTTTAGTAATCGGAGCTGGAATAATCGGGTTGACCATTGCCCGGCAGTTCCTAATCGGGTCGGACCTGTCGGTCGCCGTAATTGACAAGTCTGTCCCTTGCTCTGGCGCCACCGGTGCCGGCCAAGGGTATATATGGATGGGACACAAAACTCCCGGCAGCGACCTTTGGGACTTGGGGCTCAGAAGCCAGAAGCTTTGGGAGGAATTGGCTGAGAGTTTGATTGAGCAAGGTTTGGACCCTCTGCAACTATTGGGTTGGAAGAAAACAGGAAGCTTGCTAGTTGGTAGAACTCCTGAGGACTTAGACGTGTTGAAAAGGAGGGTTAAGCAGGTATGTGAAGCCGGGTTAAGAGCGGAGTACTTGTCTTCCAGTGACTTGCATGTAAAGGAACCTGAACTCTTGGTTGATAAAGACACTGGAGCTGCCTTTCTACCTGATGATTGCCAGTTGGATGCACGCTGTGGTGTTGAATATCTTGAACAGGGTAACAGGAATTATGCATCGAAGGGTAGATATGCTGAGTTTTATAATCATCCGGTGATTAGTTTATTAAGCTGGTGGCAAGGGGGAGGTTGTGGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

273

Amino Acids

29.42

Weight (kDa)

6.08

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 77 - 231 1e-24 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
AasI GACNNNNNNGTC 2 cut(s) 302, 323
Acc16I TGCGCA 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 553
AccB1I GGYRCC 2 cut(s) 338, 347
AccBSI CCGCTC 1 cut(s) 587
AccII CGCG 1 cut(s) 26
AciI CCGC 4 cut(s) 156, 159, 186, 587
AclWI GGATC 1 cut(s) 162
AcoI YGGCCR 2 cut(s) 172, 352
AcsI RAATTY 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 695
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 3 cut(s) 273, 335, 357
AflIII ACRYGT 1 cut(s) 540
AgeI ACCGGT 1 cut(s) 344
AgsI TTSAA 4 cut(s) 79, 547, 704, 713
AjiI CACGTC 1 cut(s) 541
AluBI AGCT 7 cut(s) 11, 90, 248, 427, 507, 656, 789
AluI AGCT 7 cut(s) 11, 90, 248, 427, 507, 656, 789
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 1 cut(s) 162
AoxI GGCC 3 cut(s) 36, 172, 352
ApeKI GCWGC 4 cut(s) 5, 8, 390, 656
ApoI RAATTY 1 cut(s) 125
AsiGI ACCGGT 1 cut(s) 344
AspLEI GCGC 3 cut(s) 28, 153, 341
AspS9I GGNCC 3 cut(s) 37, 295, 469
AsuC2I CCSGG 3 cut(s) 274, 387, 577
AsuHPI GGTGA 1 cut(s) 784
AvaII GGWCC 2 cut(s) 295, 469
AxyI CCTNAGG 1 cut(s) 528
BanI GGYRCC 2 cut(s) 338, 347
BanII GRGCYC 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 20, 402, 643
BccI CCATC 2 cut(s) 91, 364
BceAI ACGGC 1 cut(s) 293
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 3 cut(s) 274, 387, 577
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 342
BfuAI ACCTGC 1 cut(s) 553
BisI GCNGC 5 cut(s) 6, 9, 159, 391, 657
BlsI GCNGC 5 cut(s) 7, 10, 160, 392, 658
BmcAI AGTACT 1 cut(s) 593
Bme1390I CCNGG 3 cut(s) 274, 387, 577
Bme18I GGWCC 2 cut(s) 295, 469
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 3 cut(s) 37, 295, 469
BmiI GGNNCC 5 cut(s) 118, 340, 349, 471, 624
BmrFI CCNGG 3 cut(s) 274, 387, 577
BmrI ACTGGG 2 cut(s) 94, 205
BmsI GCATC 4 cut(s) 40, 140, 676, 743
BmuI ACTGGG 2 cut(s) 94, 205
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 1 cut(s) 672
BpuMI CCSGG 3 cut(s) 274, 387, 577
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 355
BsaWI WCCGGW 2 cut(s) 344, 769
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 335, 357
Bse118I RCCGGY 2 cut(s) 344, 350
Bse1I ACTGG 5 cut(s) 100, 211, 603, 655, 679
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 355
BseGI GGATG 6 cut(s) 83, 107, 131, 375, 691, 766
BseLI CCNNNNNNNGG 3 cut(s) 273, 335, 357
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 4 cut(s) 427, 435, 519, 744
BseNI ACTGG 5 cut(s) 100, 211, 603, 655, 679
BseRI GAGGAG 1 cut(s) 109
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 20, 402, 643
Bsh1236I CGCG 1 cut(s) 26
Bsh1285I CGRYCG 2 cut(s) 175, 306
BshFI GGCC 3 cut(s) 38, 174, 354
BshNI GGYRCC 2 cut(s) 338, 347
BshTI ACCGGT 1 cut(s) 344
BsiEI CGRYCG 2 cut(s) 175, 306
BsiSI CCGG 7 cut(s) 171, 274, 345, 351, 387, 576, 770
BslFI GGGAC 4 cut(s) 181, 311, 387, 416
BslI CCNNNNNNNGG 3 cut(s) 273, 335, 357
BsmAI GTCTC 1 cut(s) 61
BsmFI GGGAC 4 cut(s) 181, 311, 387, 416
BsnI GGCC 3 cut(s) 38, 174, 354
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 2 cut(s) 167, 217
BspACI CCGC 4 cut(s) 156, 159, 186, 587
BspANI GGCC 3 cut(s) 38, 174, 354
BspCNI CTCAG 4 cut(s) 426, 436, 520, 745
BspFNI CGCG 1 cut(s) 26
BspLI GGNNCC 5 cut(s) 118, 340, 349, 471, 624
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 1 cut(s) 162
BspT107I GGYRCC 2 cut(s) 338, 347
BsrBI CCGCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 267
BsrFI RCCGGY 2 cut(s) 344, 350
BsrI ACTGG 5 cut(s) 100, 211, 603, 655, 679
BssAI RCCGGY 2 cut(s) 344, 350
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 2 cut(s) 167, 217
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 355
BstACI GRCGYC 1 cut(s) 339
BstC8I GCNNGC 3 cut(s) 352, 509, 691
BstDEI CTNAG 5 cut(s) 413, 444, 528, 535, 753
BstF5I GGATG 6 cut(s) 83, 107, 131, 375, 691, 766
BstFNI CGCG 1 cut(s) 26
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 3 cut(s) 28, 153, 341
BstKTI GATC 2 cut(s) 170, 220
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 2 cut(s) 167, 217
BstMCI CGRYCG 2 cut(s) 175, 306
BstMWI GCNNNNNNNGC 2 cut(s) 148, 347
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 3 cut(s) 272, 385, 575
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 3 cut(s) 20, 402, 643
BstV2I GAAGAC 1 cut(s) 591
BstZI CGGCCG 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 528
BsuRI GGCC 3 cut(s) 38, 174, 354
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 6 cut(s) 83, 107, 131, 375, 691, 766
BtsIMutI CAGTG 2 cut(s) 610, 648
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 3 cut(s) 352, 509, 691
CfoI GCGC 3 cut(s) 28, 153, 341
Cfr10I RCCGGY 2 cut(s) 344, 350
Cfr13I GGNCC 3 cut(s) 37, 295, 469
Csp6I GTAC 1 cut(s) 592
CspAI ACCGGT 1 cut(s) 344
CviAII CATG 1 cut(s) 614
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 5 cut(s) 413, 444, 528, 535, 753
DinI GGCGCC 1 cut(s) 340
DpnI GATC 2 cut(s) 169, 219
DpnII GATC 2 cut(s) 167, 217
DraIII CACNNNGTG 1 cut(s) 695
DrdI GACNNNNNNGTC 2 cut(s) 302, 323
DseDI GACNNNNNNGTC 2 cut(s) 302, 323
EaeI YGGCCR 2 cut(s) 172, 352
EagI CGGCCG 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 355
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 2 cut(s) 295, 469
Eco52I CGGCCG 1 cut(s) 172
Eco81I CCTNAGG 1 cut(s) 528
EcoT14I CCWWGG 1 cut(s) 355
EcoT22I ATGCAT 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 414
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 355
FaeI CATG 1 cut(s) 617
FaiI YATR 8 cut(s) 363, 365, 367, 568, 615, 732, 750, 762
FaqI GGGAC 4 cut(s) 181, 311, 387, 416
FatI CATG 1 cut(s) 613
FauI CCCGC 1 cut(s) 193
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 5 cut(s) 6, 9, 159, 391, 657
FokI GGATG 6 cut(s) 70, 94, 118, 382, 698, 753
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 5 cut(s) 6, 9, 159, 391, 657
FspBI CTAG 1 cut(s) 512
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 3 cut(s) 27, 152, 340
GluI GCNGC 5 cut(s) 6, 9, 159, 391, 657
GsuI CTGGAG 1 cut(s) 672
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 3 cut(s) 38, 174, 354
HapII CCGG 7 cut(s) 171, 274, 345, 351, 387, 576, 770
HhaI GCGC 3 cut(s) 28, 153, 341
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 1 cut(s) 617
Hin6I GCGC 3 cut(s) 26, 151, 339
HinP1I GCGC 3 cut(s) 26, 151, 339
HincII GTYRAC 2 cut(s) 105, 264
HindII GTYRAC 2 cut(s) 105, 264
HindIII AAGCTT 2 cut(s) 425, 505
HinfI GANTC 1 cut(s) 206
HpaII CCGG 7 cut(s) 171, 274, 345, 351, 387, 576, 770
HphI GGTGA 1 cut(s) 784
Hpy166II GTNNAC 2 cut(s) 105, 264
Hpy188I TCNGA 6 cut(s) 68, 205, 217, 245, 295, 416
Hpy188III TCNNGA 2 cut(s) 527, 710
Hpy8I GTNNAC 2 cut(s) 105, 264
HpyAV CCTTC 1 cut(s) 733
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 6 cut(s) 8, 53, 478, 613, 689, 734
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 347
HpyF3I CTNAG 5 cut(s) 413, 444, 528, 535, 753
HpySE526I ACGT 1 cut(s) 540
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 1 cut(s) 617
HspAI GCGC 3 cut(s) 26, 151, 339
KasI GGCGCC 1 cut(s) 338
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 2 cut(s) 167, 217
LmnI GCTCC 3 cut(s) 122, 245, 653
Lsp1109I GCAGC 3 cut(s) 20, 402, 643
LweI GCATC 4 cut(s) 40, 140, 676, 743
MaeI CTAG 1 cut(s) 512
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 3 cut(s) 71, 605, 719
MalI GATC 2 cut(s) 169, 219
MbiI CCGCTC 1 cut(s) 587
MboI GATC 2 cut(s) 167, 217
MboII GAAGA 2 cut(s) 505, 591
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 4 cut(s) 125, 312, 437, 727
Mly113I GGCGCC 1 cut(s) 339
MmeI TCCRAC 3 cut(s) 273, 470, 663
MnlI CCTC 7 cut(s) 106, 130, 427, 483, 523, 546, 797
Mph1103I ATGCAT 1 cut(s) 736
MroNI GCCGGC 1 cut(s) 350
MseI TTAA 4 cut(s) 558, 581, 785, 817
MspI CCGG 7 cut(s) 171, 274, 345, 351, 387, 576, 770
MspR9I CCNGG 3 cut(s) 274, 387, 577
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 148, 347
NaeI GCCGGC 1 cut(s) 352
NarI GGCGCC 1 cut(s) 339
NciI CCSGG 3 cut(s) 274, 387, 577
NdeII GATC 2 cut(s) 167, 217
NgoMIV GCCGGC 1 cut(s) 350
NlaIII CATG 1 cut(s) 617
NlaIV GGNNCC 5 cut(s) 118, 340, 349, 471, 624
NmuCI GTSAC 2 cut(s) 71, 605
NsbI TGCGCA 1 cut(s) 152
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 1 cut(s) 617
PdiI GCCGGC 1 cut(s) 352
PfeI GAWTC 1 cut(s) 206
PinAI ACCGGT 1 cut(s) 344
PkrI GCNGC 5 cut(s) 7, 10, 160, 392, 658
PluTI GGCGCC 1 cut(s) 342
PsiI TTATAA 1 cut(s) 762
PspN4I GGNNCC 5 cut(s) 118, 340, 349, 471, 624
PspPI GGNCC 3 cut(s) 37, 295, 469
PstI CTGCAG 1 cut(s) 10
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
SaqAI TTAA 4 cut(s) 558, 581, 785, 817
SatI GCNGC 5 cut(s) 6, 9, 159, 391, 657
Sau3AI GATC 2 cut(s) 167, 217
Sau96I GGNCC 3 cut(s) 37, 295, 469
ScaI AGTACT 1 cut(s) 593
ScrFI CCNGG 3 cut(s) 274, 387, 577
SduI GDGCHC 1 cut(s) 414
SfaNI GCATC 4 cut(s) 40, 140, 676, 743
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 340
SgrAI CRCCGGYG 1 cut(s) 344
SinI GGWCC 2 cut(s) 295, 469
Sse9I AATT 4 cut(s) 125, 312, 437, 727
SsiI CCGC 4 cut(s) 156, 159, 186, 587
SspDI GGCGCC 1 cut(s) 338
SspMI CTAG 1 cut(s) 512
StyD4I CCNGG 3 cut(s) 272, 385, 575
StyI CCWWGG 1 cut(s) 355
TaiI ACGT 1 cut(s) 543
TaqI TCGA 3 cut(s) 14, 228, 737
TaqII GACCGA 1 cut(s) 292
TasI AATT 4 cut(s) 125, 312, 437, 727
TatI WGTACW 1 cut(s) 591
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 4 cut(s) 558, 581, 785, 817
Tru9I TTAA 4 cut(s) 558, 581, 785, 817
TscAI CASTG 2 cut(s) 610, 655
TseFI GTSAC 2 cut(s) 71, 605
TseI GCWGC 4 cut(s) 5, 8, 390, 656
Tsp45I GTSAC 2 cut(s) 71, 605
TspRI CASTG 2 cut(s) 610, 655
VpaK11BI GGWCC 2 cut(s) 295, 469
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 617
XspI CTAG 1 cut(s) 512
ZrmI AGTACT 1 cut(s) 593
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.