pycom05g15460

Xyloglucan glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013542
Physical Location & Seq
Forward (+)
19033114 .. 19036077
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1809 bp
ATGTCTCGAGCTCAAAACCACGAGTTTCAAGAATGGTGGAACAAGCAGAGAAAGTCCAACCACGACCTACTCCTCTCCTCCGACGAATCCAGCCACCTCCTCACCGTCGATGTACACAGCCCCGGCCCCGATCGGACTGTAGGAAAAGACCGCTCCCACAGCGCCCGCCAGCTGTCCTGGGTCTGCCTCCTCAAGTTCCAGCAAATCGCCGCATCCCTCACCTCCCTCGCCAACTCCTTCCTCGTCCTCCTCCGGACCGCCAATCGCCGCGTCAGCTCCCCTGATTCGCCCGCTGACTCGGCCTCCTCGCGCCTTTACCGCGCCATTAAGGTTTTTCTGATTGTCGTGCTGCTGCTGCTCTGCTTCGAGCTCGTGGCTTACTTCAAGGGATGGCATTTCAGTCCGCCGTCGGTGAGATCTCATGCCAAGAGACAGCTGGCGGAGCTCGTGTACGCCAATTGGCTCCACGTTCGAGTCAATTACTTGGCTCCGCCGCTGCAGAGCTTCACGAATGTCTGCATTGTGCTGTTCCTGATTCAGTCGGTGGATCGCGTTGTTTTGGTGCTCGGATGCTTCTGGATCAAGTTCCGTAGAATCAAGCCCAAGGCGGTCATGGAGTACAAAACGACGTCGTCGTCTTCCTCGAACAATGTGGAGGAGGGGAATGCTGAGAATGTGAATTTGGAGGATTATCCGATGGTGCTGCTGCAGATTCCTATGTGCAATGAGAGGGAGGTTTACCATCAATCGATTGCCGCAGTTTGTATTCAGGACTGGCCGAAGGAGAGAATGCTTGTACAGGTTTTGGACGATTCTGACGATAGAGAGGTTCAACAACTTATTAAGGCAGAAGTCAACAAGTGGCAACAACGGGGCGTTCCCATATTGTACAGACATCGGCTGATACGCACAGGGTATAAGGCAGGGAATCTGAAATCTGCTATGAGCTGCGATTATGTTAAAAATTACGAGTTTGTAGCCATATTTGATTCGGATTTTCAGCCGGAACCTGAATTCTTGAAGAAAACTGTTCCTTATTTTAAGGGGAATGATGATCTAGGCTTGGTTCAGACAAGGTGGTCTTTTGTAAACAAGGATGAGAACTTGCTTACTAGACTGCAGAACATAAACTTATCGTTCCACTTTGAGGTTGAACAACAAGTCAATGGTGTGTTTATCAACTTCTTTGGTTTTAACGGTACAGCTGGTGTGTGGAGGATTAAGGCTCTTGAGGAATGTGGTGGTTGGTTGGAACGAACTACAGTCGAGGACATGGATGTTGCAGTTCGCGCTCATCTTTGTGGGTGGAAGTTCATATATCTGAATGATGTTAAGTGCCTTTGTGAACTTCCAGAGTCCTACGGGGCATACAAGAAACAGCAACATCGCTGGCATTCTGGTCCAATGCAGTTATTCCGCATGTGTTTCTTTGACATACTCCGTTCGAAGGTGAGTGTGACTAAGAAAGCGAATTTGATATTCCTCTTCTTCCTCCTGCGAAAGCTCATACTGCCGTTTTATTCGTTCACTCTCTTTTGCATCATTCTTCCACTGACAATGTTCCTGCCAGAAGCTCAGCTACCAGCATGGGTTGTTTGCTATGTTCCTGGAATTATGTCTGTCTTGAATATCCTTCCTGCACCACGTTCTTTCCCATTTATCGTCCCCTACCTTCTGTTTGAGAATACCATGTCGGTGACGAAATTTAATGCCATGATATCAGGATTGTTTCAATTTGGGAGTTCTTATGAGTGGATAGTTACAAAGAAATTGGGAAGATCGATCAGAGACAGATCTAATTGCTTTTGA

Protein Analysis

603

Amino Acids

69.67

Weight (kDa)

9.04

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 233 - 465 5.6e-14 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 238 - 373 2e-08 Glycosyl transferase family 2
Glyco_transf_21 PF13506 321 - 465 8.6e-06 Glycosyl transferase family 21
Glyco_trans_2_3 PF13632 326 - 522 2.6e-16 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013542)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07330 AT3G07330
fragaria_vesca FvH4_2g18120
malus_domestica MD05G1166000.v1.1 MD10G1156200.v1.1
prunus_persica Prupe.8G194000_v2.0.a1
pyrus_communis pycom05g15460 pycom10g13440
rosa_chinensis RchiOBHm_Chr6g0283521
rosa_laevigata RLG00000012820
rosa_multiflora Rmu_sc0005719.1_g000002 Rmu_sc0007194.1_g000007
rosa_roxburghii Rroxscaffold_7G00185470
rosa_rugosa Rorug06G0155100
rosa_samantha Rh6AG267300 Rh6BG269800 Rh6CG269600
rosa_wichuraiana Rw6G023140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 634, 1078
AatII GACGTC 1 cut(s) 632
AccBSI CCGCTC 1 cut(s) 153
AccII CGCG 5 cut(s) 270, 310, 321, 552, 1290
AccIII TCCGGA 1 cut(s) 252
AclWI GGATC 2 cut(s) 555, 587
AcoI YGGCCR 1 cut(s) 776
AcsI RAATTY 4 cut(s) 679, 1013, 1471, 1703
AcyI GRCGYC 1 cut(s) 629
AfaI GTAC 6 cut(s) 114, 452, 620, 798, 890, 1201
AfiI CCNNNNNNNGG 2 cut(s) 1147, 1447
AgsI TTSAA 7 cut(s) 29, 385, 833, 1021, 1154, 1627, 1733
AjnI CCWGG 2 cut(s) 176, 1606
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
Alw21I GWGCWC 4 cut(s) 13, 372, 447, 567
Alw26I GTCTC 3 cut(s) 9, 424, 1782
AlwI GGATC 2 cut(s) 555, 587
Ama87I CYCGRG 1 cut(s) 6
Aor13HI TCCGGA 1 cut(s) 252
AoxI GGCC 3 cut(s) 124, 300, 776
ApeKI GCWGC 7 cut(s) 349, 352, 355, 496, 703, 706, 948
ApoI RAATTY 4 cut(s) 679, 1013, 1471, 1703
AspLEI GCGC 4 cut(s) 164, 312, 323, 1292
AspS9I GGNCC 3 cut(s) 125, 255, 1400
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 5 cut(s) 94, 211, 424, 1462, 1708
AsuII TTCGAA 1 cut(s) 1445
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 2 cut(s) 255, 1400
BanII GRGCYC 3 cut(s) 13, 372, 447
BarI GAAGNNNNNNTAC 2 cut(s) 1563, 1595
BauI CACGAG 3 cut(s) 20, 371, 446
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 4 cut(s) 13, 372, 447, 567
BbvI GCAGC 7 cut(s) 336, 339, 342, 483, 690, 693, 935
BccI CCATC 3 cut(s) 384, 691, 750
BceAI ACGGC 2 cut(s) 391, 1498
BcgI CGANNNNNNTGC 2 cut(s) 685, 719
BciT130I CCWGG 2 cut(s) 178, 1608
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 3 cut(s) 9, 424, 1782
BfaI CTAG 2 cut(s) 1058, 1113
BfmI CTRYAG 5 cut(s) 138, 497, 707, 1118, 1260
BfoI RGCGCY 1 cut(s) 165
BglII AGATCT 2 cut(s) 416, 1793
BlpI GCTNAGC 1 cut(s) 1575
Bme1390I CCNGG 3 cut(s) 123, 178, 1608
Bme18I GGWCC 2 cut(s) 255, 1400
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 3 cut(s) 125, 255, 1400
BmiI GGNNCC 4 cut(s) 127, 464, 489, 1008
BmrFI CCNGG 3 cut(s) 123, 178, 1608
BmsI GCATC 3 cut(s) 221, 560, 1548
BpiI GAAGAC 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 1575
Bpu14I TTCGAA 1 cut(s) 1445
BpuEI CTTGAG 2 cut(s) 176, 1250
BpuMI CCSGG 1 cut(s) 123
Bsa29I ATCGAT 2 cut(s) 749, 1781
BsaHI GRCGYC 1 cut(s) 629
BsaJI CCNNGG 3 cut(s) 121, 177, 603
BsaWI WCCGGW 1 cut(s) 252
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 434, 464
Bsc4I CCNNNNNNNGG 2 cut(s) 1147, 1447
Bse1I ACTGG 1 cut(s) 779
Bse3DI GCAATG 1 cut(s) 730
BseAI TCCGGA 1 cut(s) 252
BseBI CCWGG 2 cut(s) 178, 1608
BseCI ATCGAT 2 cut(s) 749, 1781
BseDI CCNNGG 3 cut(s) 121, 177, 603
BseGI GGATG 5 cut(s) 212, 395, 575, 1102, 1282
BseLI CCNNNNNNNGG 2 cut(s) 1147, 1447
BseMI GCAATG 1 cut(s) 730
BseMII CTCAG 2 cut(s) 660, 1589
BseNI ACTGG 1 cut(s) 779
BseRI GAGGAG 7 cut(s) 62, 67, 89, 179, 239, 295, 671
BseXI GCAGC 7 cut(s) 336, 339, 342, 483, 690, 693, 935
BsgI GTGCAG 1 cut(s) 1623
Bsh1236I CGCG 5 cut(s) 270, 310, 321, 552, 1290
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 3 cut(s) 126, 302, 778
BshVI ATCGAT 2 cut(s) 749, 1781
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 4 cut(s) 13, 372, 447, 567
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 3 cut(s) 123, 253, 1004
BslFI GGGAC 1 cut(s) 1649
BslI CCNNNNNNNGG 2 cut(s) 1147, 1447
BsmAI GTCTC 3 cut(s) 9, 424, 1782
BsmFI GGGAC 1 cut(s) 1649
BsmI GAATGC 3 cut(s) 670, 795, 1393
BsnI GGCC 3 cut(s) 126, 302, 778
BsoBI CYCGRG 1 cut(s) 6
Bsp119I TTCGAA 1 cut(s) 1445
Bsp1286I GDGCHC 4 cut(s) 13, 372, 447, 567
Bsp13I TCCGGA 1 cut(s) 252
Bsp1407I TGTACA 3 cut(s) 112, 796, 888
Bsp143I GATC 8 cut(s) 130, 416, 547, 579, 1054, 1778, 1782, 1793
Bsp1720I GCTNAGC 1 cut(s) 1575
BspANI GGCC 3 cut(s) 126, 302, 778
BspCNI CTCAG 2 cut(s) 661, 1588
BspDI ATCGAT 2 cut(s) 749, 1781
BspEI TCCGGA 1 cut(s) 252
BspFNI CGCG 5 cut(s) 270, 310, 321, 552, 1290
BspLI GGNNCC 4 cut(s) 127, 464, 489, 1008
BspMAI CTGCAG 3 cut(s) 501, 711, 1122
BspPI GGATC 2 cut(s) 555, 587
BspT104I TTCGAA 1 cut(s) 1445
BsrBI CCGCTC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 730
BsrGI TGTACA 3 cut(s) 112, 796, 888
BsrI ACTGG 1 cut(s) 779
BssECI CCNNGG 3 cut(s) 121, 177, 603
BssMI GATC 8 cut(s) 130, 416, 547, 579, 1054, 1778, 1782, 1793
BssNI GRCGYC 1 cut(s) 629
BssSI CACGAG 3 cut(s) 20, 371, 446
BssT1I CCWWGG 1 cut(s) 603
Bst2BI CACGAG 3 cut(s) 20, 371, 446
Bst2UI CCWGG 2 cut(s) 178, 1608
Bst4CI ACNGT 5 cut(s) 106, 139, 1030, 1199, 1264
Bst6I CTCTTC 1 cut(s) 1490
BstACI GRCGYC 1 cut(s) 629
BstAUI TGTACA 3 cut(s) 112, 796, 888
BstBI TTCGAA 1 cut(s) 1445
BstC8I GCNNGC 5 cut(s) 166, 170, 291, 438, 1391
BstDEI CTNAG 3 cut(s) 669, 1461, 1575
BstF5I GGATG 5 cut(s) 212, 395, 575, 1102, 1282
BstFNI CGCG 5 cut(s) 270, 310, 321, 552, 1290
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 164, 312, 323, 1292
BstKTI GATC 8 cut(s) 133, 419, 550, 582, 1057, 1781, 1785, 1796
BstMAI GTCTC 3 cut(s) 9, 424, 1782
BstMBI GATC 8 cut(s) 130, 416, 547, 579, 1054, 1778, 1782, 1793
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 8 cut(s) 159, 273, 299, 318, 355, 442, 1289, 1510
BstNI CCWGG 2 cut(s) 178, 1608
BstNSI RCATGY 1 cut(s) 1423
BstSCI CCNGG 3 cut(s) 121, 176, 1606
BstSFI CTRYAG 5 cut(s) 138, 497, 707, 1118, 1260
BstUI CGCG 5 cut(s) 270, 310, 321, 552, 1290
BstV1I GCAGC 7 cut(s) 336, 339, 342, 483, 690, 693, 935
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 2 cut(s) 416, 1793
BstYI RGATCY 2 cut(s) 416, 1793
Bsu15I ATCGAT 2 cut(s) 749, 1781
BsuRI GGCC 3 cut(s) 126, 302, 778
BsuTUI ATCGAT 2 cut(s) 749, 1781
BtgZI GCGATG 1 cut(s) 1370
BtsCI GGATG 5 cut(s) 212, 395, 575, 1102, 1282
BtsIMutI CAGTG 1 cut(s) 1550
Cac8I GCNNGC 5 cut(s) 166, 170, 291, 438, 1391
CfoI GCGC 4 cut(s) 164, 312, 323, 1292
Cfr13I GGNCC 3 cut(s) 125, 255, 1400
ClaI ATCGAT 2 cut(s) 749, 1781
CpoI CGGWCCG 1 cut(s) 255
CseI GACGC 1 cut(s) 259
Csp6I GTAC 6 cut(s) 113, 451, 619, 797, 889, 1200
CspCI CAANNNNNGTGG 2 cut(s) 17, 52
CspI CGGWCCG 1 cut(s) 255
CviAII CATG 7 cut(s) 422, 613, 1273, 1420, 1587, 1690, 1714
CviQI GTAC 6 cut(s) 113, 451, 619, 797, 889, 1200
DdeI CTNAG 3 cut(s) 669, 1461, 1575
DpnI GATC 8 cut(s) 132, 418, 549, 581, 1056, 1780, 1784, 1795
DpnII GATC 8 cut(s) 130, 416, 547, 579, 1054, 1778, 1782, 1793
DrdI GACNNNNNNGTC 2 cut(s) 634, 1078
DseDI GACNNNNNNGTC 2 cut(s) 634, 1078
EaeI YGGCCR 1 cut(s) 776
Eam1104I CTCTTC 1 cut(s) 1490
EarI CTCTTC 1 cut(s) 1490
EciI GGCGGA 3 cut(s) 393, 455, 480
Ecl136II GAGCTC 3 cut(s) 11, 370, 445
Eco130I CCWWGG 1 cut(s) 603
Eco24I GRGCYC 3 cut(s) 13, 372, 447
Eco32I GATATC 1 cut(s) 1719
Eco47I GGWCC 2 cut(s) 255, 1400
Eco53kI GAGCTC 3 cut(s) 11, 370, 445
Eco88I CYCGRG 1 cut(s) 6
EcoICRI GAGCTC 3 cut(s) 11, 370, 445
EcoRI GAATTC 1 cut(s) 1013
EcoRII CCWGG 2 cut(s) 176, 1606
EcoRV GATATC 1 cut(s) 1719
EcoT14I CCWWGG 1 cut(s) 603
EcoT38I GRGCYC 3 cut(s) 13, 372, 447
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 7 cut(s) 425, 616, 1276, 1423, 1590, 1693, 1717
FalI AAGNNNNNCTT 2 cut(s) 1066, 1098
FaqI GGGAC 1 cut(s) 1649
FatI CATG 7 cut(s) 421, 612, 1272, 1419, 1586, 1689, 1713
FauI CCCGC 2 cut(s) 173, 298
FokI GGATG 5 cut(s) 199, 402, 582, 1109, 1289
FriOI GRGCYC 3 cut(s) 13, 372, 447
FspBI CTAG 2 cut(s) 1058, 1113
GlaI GCGC 4 cut(s) 163, 311, 322, 1291
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 126, 302, 778
HapII CCGG 3 cut(s) 123, 253, 1004
HgaI GACGC 1 cut(s) 259
HhaI GCGC 4 cut(s) 164, 312, 323, 1292
Hin1I GRCGYC 1 cut(s) 629
Hin1II CATG 7 cut(s) 425, 616, 1276, 1423, 1590, 1693, 1717
Hin6I GCGC 4 cut(s) 162, 310, 321, 1290
HinP1I GCGC 4 cut(s) 162, 310, 321, 1290
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HpaII CCGG 3 cut(s) 123, 253, 1004
HphI GGTGA 5 cut(s) 94, 211, 424, 1462, 1708
Hpy166II GTNNAC 7 cut(s) 115, 451, 739, 856, 1090, 1346, 1527
Hpy8I GTNNAC 7 cut(s) 115, 451, 739, 856, 1090, 1346, 1527
Hpy99I CGWCG 6 cut(s) 86, 110, 412, 631, 634, 637
HpyAV CCTTC 5 cut(s) 247, 775, 1441, 1643, 1682
HpyCH4III ACNGT 5 cut(s) 106, 139, 1030, 1199, 1264
HpyCH4IV ACGT 3 cut(s) 468, 629, 1645
HpyCH4V TGCA 9 cut(s) 499, 519, 709, 723, 1120, 1283, 1408, 1539, 1640
HpyF10VI GCNNNNNNNGC 8 cut(s) 159, 273, 299, 318, 355, 442, 1289, 1510
HpyF3I CTNAG 3 cut(s) 669, 1461, 1575
HpySE526I ACGT 3 cut(s) 468, 629, 1645
Hsp92I GRCGYC 1 cut(s) 629
Hsp92II CATG 7 cut(s) 425, 616, 1276, 1423, 1590, 1693, 1717
HspAI GCGC 4 cut(s) 162, 310, 321, 1290
Kpn2I TCCGGA 1 cut(s) 252
Kzo9I GATC 8 cut(s) 130, 416, 547, 579, 1054, 1778, 1782, 1793
LmnI GCTCC 5 cut(s) 158, 281, 442, 468, 493
Lsp1109I GCAGC 7 cut(s) 336, 339, 342, 483, 690, 693, 935
LweI GCATC 3 cut(s) 221, 560, 1548
MaeI CTAG 2 cut(s) 1058, 1113
MaeII ACGT 3 cut(s) 468, 629, 1645
MaeIII GTNAC 3 cut(s) 1456, 1696, 1759
MalI GATC 8 cut(s) 132, 418, 549, 581, 1056, 1780, 1784, 1795
MbiI CCGCTC 1 cut(s) 153
MboI GATC 8 cut(s) 130, 416, 547, 579, 1054, 1778, 1782, 1793
MboII GAAGA 6 cut(s) 630, 1033, 1477, 1480, 1538, 1788
MfeI CAATTG 1 cut(s) 457
MflI RGATCY 2 cut(s) 416, 1793
MhlI GDGCHC 4 cut(s) 13, 372, 447, 567
MlyI GAGTC 3 cut(s) 290, 483, 1364
MmeI TCCRAC 3 cut(s) 81, 105, 1230
MroI TCCGGA 1 cut(s) 252
MseI TTAA 8 cut(s) 327, 843, 960, 1041, 1194, 1221, 1332, 1707
MslI CAYNNNNRTG 2 cut(s) 1299, 1694
MspA1I CMGCKG 5 cut(s) 172, 293, 436, 496, 1205
MspI CCGG 3 cut(s) 123, 253, 1004
MspR9I CCNGG 3 cut(s) 123, 178, 1608
MunI CAATTG 1 cut(s) 457
Mva1269I GAATGC 3 cut(s) 670, 795, 1393
MvaI CCWGG 2 cut(s) 178, 1608
MvnI CGCG 5 cut(s) 270, 310, 321, 552, 1290
MwoI GCNNNNNNNGC 8 cut(s) 159, 273, 299, 318, 355, 442, 1289, 1510
NciI CCSGG 1 cut(s) 123
NdeII GATC 8 cut(s) 130, 416, 547, 579, 1054, 1778, 1782, 1793
NlaIII CATG 7 cut(s) 425, 616, 1276, 1423, 1590, 1693, 1717
NlaIV GGNNCC 4 cut(s) 127, 464, 489, 1008
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 2 cut(s) 1456, 1696
NspI RCATGY 1 cut(s) 1423
NspV TTCGAA 1 cut(s) 1445
PaeR7I CTCGAG 1 cut(s) 6
PcsI WCGNNNNNNNCGW 3 cut(s) 632, 641, 816
PctI GAATGC 3 cut(s) 670, 795, 1393
PfeI GAWTC 8 cut(s) 86, 284, 535, 594, 712, 812, 928, 989
PflFI GACNNNGTC 1 cut(s) 631
PfoI TCCNGGA 1 cut(s) 1606
Ple19I CGATCG 1 cut(s) 133
PleI GAGTC 3 cut(s) 290, 482, 1363
PpsI GAGTC 3 cut(s) 290, 482, 1363
Psp124BI GAGCTC 3 cut(s) 13, 372, 447
Psp6I CCWGG 2 cut(s) 176, 1606
PspGI CCWGG 2 cut(s) 176, 1606
PspN4I GGNNCC 4 cut(s) 127, 464, 489, 1008
PspPI GGNCC 3 cut(s) 125, 255, 1400
PstI CTGCAG 3 cut(s) 501, 711, 1122
PsuI RGATCY 2 cut(s) 416, 1793
PsyI GACNNNGTC 1 cut(s) 631
PvuI CGATCG 1 cut(s) 133
PvuII CAGCTG 3 cut(s) 172, 436, 1205
RsaI GTAC 6 cut(s) 114, 452, 620, 798, 890, 1201
RsaNI GTAC 6 cut(s) 113, 451, 619, 797, 889, 1200
RseI CAYNNNNRTG 2 cut(s) 1299, 1694
Rsr2I CGGWCCG 1 cut(s) 255
RsrII CGGWCCG 1 cut(s) 255
SacI GAGCTC 3 cut(s) 13, 372, 447
SaqAI TTAA 8 cut(s) 327, 843, 960, 1041, 1194, 1221, 1332, 1707
Sau3AI GATC 8 cut(s) 130, 416, 547, 579, 1054, 1778, 1782, 1793
Sau96I GGNCC 3 cut(s) 125, 255, 1400
SchI GAGTC 3 cut(s) 290, 483, 1364
ScrFI CCNGG 3 cut(s) 123, 178, 1608
SduI GDGCHC 4 cut(s) 13, 372, 447, 567
SfaNI GCATC 3 cut(s) 221, 560, 1548
SfcI CTRYAG 5 cut(s) 138, 497, 707, 1118, 1260
Sfr274I CTCGAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 1445
SinI GGWCC 2 cut(s) 255, 1400
SlaI CTCGAG 1 cut(s) 6
SmiMI CAYNNNNRTG 2 cut(s) 1299, 1694
SmlI CTYRAG 3 cut(s) 6, 191, 1229
SmoI CTYRAG 3 cut(s) 6, 191, 1229
SspMI CTAG 2 cut(s) 1058, 1113
SstI GAGCTC 3 cut(s) 13, 372, 447
StyD4I CCNGG 3 cut(s) 121, 176, 1606
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 5 cut(s) 106, 139, 1030, 1199, 1264
TaiI ACGT 3 cut(s) 471, 632, 1648
TaqI TCGA 9 cut(s) 7, 108, 366, 472, 644, 749, 1266, 1445, 1781
TatI WGTACW 4 cut(s) 112, 618, 796, 888
TauI GCSGC 4 cut(s) 212, 270, 496, 758
TfiI GAWTC 8 cut(s) 86, 284, 535, 594, 712, 812, 928, 989
Tru1I TTAA 8 cut(s) 327, 843, 960, 1041, 1194, 1221, 1332, 1707
Tru9I TTAA 8 cut(s) 327, 843, 960, 1041, 1194, 1221, 1332, 1707
TscAI CASTG 1 cut(s) 1557
TseFI GTSAC 2 cut(s) 1456, 1696
TseI GCWGC 7 cut(s) 349, 352, 355, 496, 703, 706, 948
Tsp45I GTSAC 2 cut(s) 1456, 1696
TspDTI ATGAA 1 cut(s) 1303
TspGWI ACGGA 2 cut(s) 578, 1430
TspRI CASTG 1 cut(s) 1557
Tth111I GACNNNGTC 1 cut(s) 631
VpaK11BI GGWCC 2 cut(s) 255, 1400
XapI RAATTY 4 cut(s) 679, 1013, 1471, 1703
XceI RCATGY 1 cut(s) 1423
XcmI CCANNNNNNNNNTGG 2 cut(s) 433, 610
XhoI CTCGAG 1 cut(s) 6
XspI CTAG 2 cut(s) 1058, 1113
ZraI GACGTC 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.