pycom05g15940

GPI transamidase component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009321
Physical Location & Seq
Forward (+)
19372237 .. 19372932
696 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15940.3

Sequence Viewer

Length: 435 bp
ATGGCGGAAATCACTGAACCTCTCCTCCCTCCTAACGAAACCCCACAAACATCCAAACCGCTAAAGCCAGCAGATGACTTGGATCCCAAGACGATGCGCAGAATCACGCGAGGACTCAAGCGCCTGATCATCACCGTTTCAGTCCTCTTCTCCTTCATTCTCGGAATCCCATTGATATACAAATCCGTGGAAATCCCATTGATTTCTAAGGCTAATAGCTTTGCATCTCCAAGGTTCTCCTGGCAGCTCTACGAGAATGGAGAAGATACAAGCAAGAAAATAAGAGACCAAGTGGTAAGGAAGGGATGCATCGAAAAGGGAGAAGATGACCTTCTTGAGTACGTGCCATTGAATTTGAGGAGGAGGCTGCGGTGTTCAGGGCGGCGGAATTATTTGTTAGTGTTAGCCGTTCGGTTCCCTTTTCAGATTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-S

Protein Analysis

145

Amino Acids

16.61

Weight (kDa)

9.77

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 98
AccII CGCG 1 cut(s) 109
AciI CCGC 5 cut(s) 5, 59, 370, 382, 385
AclWI GGATC 2 cut(s) 77, 90
AcsI RAATTY 1 cut(s) 352
AfaI GTAC 1 cut(s) 341
AgsI TTSAA 1 cut(s) 352
AjnI CCWGG 1 cut(s) 239
AloI GAACNNNNNNTCC 2 cut(s) 9, 41
AluBI AGCT 2 cut(s) 219, 247
AluI AGCT 2 cut(s) 219, 247
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 2 cut(s) 77, 90
ApeKI GCWGC 2 cut(s) 244, 367
ApoI RAATTY 1 cut(s) 352
AspLEI GCGC 2 cut(s) 99, 123
AsuHPI GGTGA 1 cut(s) 124
BamHI GGATCC 1 cut(s) 82
BbvI GCAGC 2 cut(s) 256, 354
BceAI ACGGC 1 cut(s) 392
BciT130I CCWGG 1 cut(s) 241
BclI TGATCA 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 124
BisI GCNGC 3 cut(s) 245, 368, 383
BlsI GCNGC 3 cut(s) 246, 369, 384
Bme1390I CCNGG 1 cut(s) 241
BmiI GGNNCC 2 cut(s) 84, 416
BmrFI CCNGG 1 cut(s) 241
BmsI GCATC 4 cut(s) 84, 233, 296, 318
BpuEI CTTGAG 2 cut(s) 101, 356
BsaAI YACGTR 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 186, 230
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 186, 230
BseGI GGATG 2 cut(s) 50, 311
BseRI GAGGAG 3 cut(s) 14, 373, 376
BseXI GCAGC 2 cut(s) 256, 354
Bsh1236I CGCG 1 cut(s) 109
BsmAI GTCTC 1 cut(s) 279
Bso31I GGTCTC 1 cut(s) 279
Bsp143I GATC 2 cut(s) 82, 126
BspACI CCGC 5 cut(s) 5, 59, 370, 382, 385
BspFNI CGCG 1 cut(s) 109
BspLI GGNNCC 2 cut(s) 84, 416
BspPI GGATC 2 cut(s) 77, 90
BspTNI GGTCTC 1 cut(s) 279
BssECI CCNNGG 2 cut(s) 186, 230
BssMI GATC 2 cut(s) 82, 126
BssT1I CCWWGG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 152
BstBAI YACGTR 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 207
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 50, 311
BstFNI CGCG 1 cut(s) 109
BstH2I RGCGCY 1 cut(s) 124
BstHHI GCGC 2 cut(s) 99, 123
BstKTI GATC 2 cut(s) 85, 129
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 2 cut(s) 82, 126
BstNI CCWGG 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 239
BstUI CGCG 1 cut(s) 109
BstV1I GCAGC 2 cut(s) 256, 354
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 50, 311
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 1 cut(s) 69
CfoI GCGC 2 cut(s) 99, 123
Csp6I GTAC 1 cut(s) 340
CviJI RGCY 6 cut(s) 67, 212, 219, 247, 367, 407
CviKI_1 RGCY 6 cut(s) 67, 212, 219, 247, 367, 407
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 1 cut(s) 207
DpnI GATC 2 cut(s) 84, 128
DpnII GATC 2 cut(s) 82, 126
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
EciI GGCGGA 2 cut(s) 20, 400
Eco130I CCWWGG 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 230
FaiI YATR 1 cut(s) 178
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FbaI TGATCA 1 cut(s) 126
Fnu4HI GCNGC 3 cut(s) 245, 368, 383
FokI GGATG 2 cut(s) 37, 318
Fsp4HI GCNGC 3 cut(s) 245, 368, 383
FspI TGCGCA 1 cut(s) 98
GlaI GCGC 2 cut(s) 98, 122
GluI GCNGC 3 cut(s) 245, 368, 383
HaeII RGCGCY 1 cut(s) 124
HhaI GCGC 2 cut(s) 99, 123
Hin6I GCGC 2 cut(s) 97, 121
HinP1I GCGC 2 cut(s) 97, 121
HinfI GANTC 4 cut(s) 102, 114, 165, 427
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 164, 426
Hpy188III TCNNGA 1 cut(s) 335
HpyAV CCTTC 3 cut(s) 163, 295, 341
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 2 cut(s) 224, 309
HpyF3I CTNAG 1 cut(s) 207
HpySE526I ACGT 1 cut(s) 342
HspAI GCGC 2 cut(s) 97, 121
Ksp22I TGATCA 1 cut(s) 126
Kzo9I GATC 2 cut(s) 82, 126
LpnPI CCDG 5 cut(s) 81, 137, 226, 253, 363
Lsp1109I GCAGC 2 cut(s) 256, 354
LweI GCATC 4 cut(s) 84, 233, 296, 318
MaeII ACGT 1 cut(s) 342
MalI GATC 2 cut(s) 84, 128
MboI GATC 2 cut(s) 82, 126
MboII GAAGA 3 cut(s) 139, 275, 335
MflI RGATCY 1 cut(s) 82
MluCI AATT 2 cut(s) 352, 388
MlyI GAGTC 1 cut(s) 108
MnlI CCTC 8 cut(s) 30, 35, 39, 104, 155, 351, 354, 357
Mph1103I ATGCAT 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 241
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 1 cut(s) 109
NdeII GATC 2 cut(s) 82, 126
NlaIV GGNNCC 2 cut(s) 84, 416
NsbI TGCGCA 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 311
PfeI GAWTC 3 cut(s) 102, 165, 427
PkrI GCNGC 3 cut(s) 246, 369, 384
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Ppu21I YACGTR 1 cut(s) 343
Psp6I CCWGG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 2 cut(s) 84, 416
PsuI RGATCY 1 cut(s) 82
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SatI GCNGC 3 cut(s) 245, 368, 383
Sau3AI GATC 2 cut(s) 82, 126
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 241
SetI ASST 6 cut(s) 22, 221, 236, 249, 333, 345
SfaNI GCATC 4 cut(s) 84, 233, 296, 318
SmlI CTYRAG 2 cut(s) 116, 335
SmoI CTYRAG 2 cut(s) 116, 335
Sse9I AATT 2 cut(s) 352, 388
SsiI CCGC 5 cut(s) 5, 59, 370, 382, 385
StyD4I CCNGG 1 cut(s) 239
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 1 cut(s) 345
TaqI TCGA 1 cut(s) 312
TasI AATT 2 cut(s) 352, 388
TauI GCSGC 1 cut(s) 385
TfiI GAWTC 3 cut(s) 102, 165, 427
TscAI CASTG 1 cut(s) 19
TseI GCWGC 2 cut(s) 244, 367
TspDTI ATGAA 2 cut(s) 145, 419
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 1 cut(s) 19
XapI RAATTY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 237
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.