Rh6CG277300

GPI transamidase component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009321
Physical Location & Seq
Forward (+)
45361586 .. 45368430
6845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG277300.1

Sequence Viewer

Length: 1356 bp
ATGGCGGAAATCACAGAACCTCTCATCCCTCCAAATCAGACACCTCAAGCAGAAGCAGATGAACCGCCTCCAGACTTCGATCCCGAAACCATGCGTAAAACCAAGCCAGGCCTCAAGCGCCTCATCCTAACCCTCTCTGTCCTCTTCTCCTTCATTTTAGGCGTCCCGTTAATATACAAATCCGTCGAAATTTACCGCGCGCCATTACCCTTCCTCGAAATCGATTCTCTCTCAGCTGAAATCGATTCCAATCCGCTTCTATTTCCATGTCGTTTCCAAGCAATCTTCCTCGGCTTCGACTCCAAATCCTCCATTACCTCTCTGGAATCTTCCATTCTTCACCAAATGACCGAATTGACTCGCCAAACGCCTCAATGCGGCACCTGTAGCCCCAACCACAGCGTCTCAGTAGTCCTGGACTCCGATTCCCACCGTCTCGTCGGTGCCGTTGATTTCGACGGCGATGATGAGGCTGTGGATGAGGCTCTGCAGGCTGTGTTTGGGGGTGGTTCGGGCTCCGGCTCCGGCGGAGAGGTGTATACGGTGGTGGTGGTGAATAGGGGTGGGGAGGAGGTGAGAGCCGTGGTTGGCAAGTATAGGAATGCTTGGATTCTCGGTAGGGTTTCGGAGGCGGAGGCGGTGGCAAAGGCGGTGGAGGTGTTTGTTAAAGTGTTTGTGAATGGTGGGAAGGAGGAAGGATTGATTCATGGCGAGTTTATGCCTGTTGGTGCTGATGGAACGCTTGTTCTGTCGTTCAATTTGCTAAATGCAGACCCTCGAGATTGGATTTATGATTGGGACTTTCAGACAGTAGATGAGACTCTGTTGGCTCCAATGATTGAGGCTATGAAACCTGTAGCAAACATAAGCGTGGAAAGTCAGGTGTTATACCATGTGCCGAAGTCCTCACTGTCTTACTGGGATGATAACTGGGACAGCTACATTTTCAGTACCAAGGATCTTCCTTTCTTTGTAAATTCGAACGAATGGCACTTGGATACATCCATTGCAGCTGGTGGGAGGTCAAAGATGTTGCACTTTGTGGTATATGTACCATCTGCAAAGGAGTGCCCTCTTCTTCTACAGCTTCCAGATGGGCAGATTTCCAAGACAAATAGCTTTATATCTCCAATGTGGGGAGGTGTTATTGTGTTGAATCCCCAATCATGCAGAAAGAATTCAGAAAGTAAGCATCCTTCTAGGCATACAATTTTGCATGAGGATCTGCAGAAGGTTTTTGAGGTTTTCATGGGACAGTTCCGCCAACTTTTTAGTTTTAAATCTGATAACTTGTATGTTGGGGCTTCAGGCACGTACAACCTTTTACCTAGTGAAAGAGGCTTCACAGTATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-S

Protein Analysis

451

Amino Acids

49.88

Weight (kDa)

4.83

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-S PF10510 46 - 430 2.8e-76 Phosphatidylinositol-glycan biosynthesis class S protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 380, 443
AccI GTMKAC 1 cut(s) 539
AccII CGCG 2 cut(s) 198, 200
AclWI GGATC 3 cut(s) 74, 966, 1230
AcsI RAATTY 3 cut(s) 189, 976, 1177
AcuI CTGAAG 1 cut(s) 1290
AcyI GRCGYC 1 cut(s) 162
AdeI CACNNNGTG 1 cut(s) 1042
AfaI GTAC 3 cut(s) 952, 1053, 1316
AfiI CCNNNNNNNGG 2 cut(s) 377, 1136
AgsI TTSAA 2 cut(s) 757, 1156
AjnI CCWGG 2 cut(s) 106, 414
AloI GAACNNNNNNTCC 4 cut(s) 9, 41, 729, 761
AluBI AGCT 5 cut(s) 236, 939, 1013, 1087, 1119
AluI AGCT 5 cut(s) 236, 939, 1013, 1087, 1119
Alw26I GTCTC 3 cut(s) 409, 440, 812
AlwI GGATC 3 cut(s) 74, 966, 1230
Ama87I CYCGRG 1 cut(s) 777
AoxI GGCC 1 cut(s) 109
ApeKI GCWGC 1 cut(s) 1010
ApoI RAATTY 3 cut(s) 189, 976, 1177
Asp700I GAANNNNTTC 1 cut(s) 1177
AspLEI GCGC 3 cut(s) 120, 200, 202
AsuHPI GGTGA 3 cut(s) 332, 565, 586
AsuII TTCGAA 1 cut(s) 980
AvaI CYCGRG 1 cut(s) 777
BaeGI GKGCMC 1 cut(s) 1073
BanI GGYRCC 2 cut(s) 380, 443
BanII GRGCYC 1 cut(s) 518
BarI GAAGNNNNNNTAC 2 cut(s) 1180, 1212
BbvI GCAGC 1 cut(s) 1022
BccI CCATC 3 cut(s) 728, 1063, 1088
BceAI ACGGC 3 cut(s) 431, 475, 566
BciT130I CCWGG 2 cut(s) 108, 416
BciVI GTATCC 1 cut(s) 991
BcoDI GTCTC 3 cut(s) 409, 440, 812
BfaI CTAG 2 cut(s) 1200, 1329
BfmI CTRYAG 5 cut(s) 385, 488, 855, 1082, 1226
BfoI RGCGCY 1 cut(s) 121
BfuI GTATCC 1 cut(s) 991
BisI GCNGC 2 cut(s) 379, 1011
BlsI GCNGC 2 cut(s) 380, 1012
Bme1390I CCNGG 2 cut(s) 108, 416
BmeT110I CYCGRG 1 cut(s) 777
BmiI GGNNCC 5 cut(s) 382, 445, 517, 523, 831
BmrFI CCNGG 2 cut(s) 108, 416
BmrI ACTGGG 2 cut(s) 928, 940
BmsI GCATC 1 cut(s) 1201
BmuI ACTGGG 2 cut(s) 928, 940
BpmI CTGGAG 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 980
BpuEI CTTGAG 2 cut(s) 30, 98
Bsa29I ATCGAT 2 cut(s) 222, 243
BsaAI YACGTR 1 cut(s) 1314
BsaBI GATNNNNATC 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 289, 582, 954
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 1136
Bse1I ACTGG 2 cut(s) 923, 935
Bse3DI GCAATG 1 cut(s) 1005
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 2 cut(s) 108, 416
BseCI ATCGAT 2 cut(s) 222, 243
BseDI CCNNGG 3 cut(s) 289, 582, 954
BseGI GGATG 6 cut(s) 24, 123, 484, 928, 1001, 1192
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 377, 1136
BseMI GCAATG 1 cut(s) 1005
BseMII CTCAG 2 cut(s) 246, 420
BseNI ACTGG 2 cut(s) 923, 935
BsePI GCGCGC 1 cut(s) 198
BseRI GAGGAG 1 cut(s) 584
BseSI GKGCMC 1 cut(s) 1073
BseXI GCAGC 1 cut(s) 1022
Bsh1236I CGCG 2 cut(s) 198, 200
BshFI GGCC 1 cut(s) 111
BshNI GGYRCC 2 cut(s) 380, 443
BshVI ATCGAT 2 cut(s) 222, 243
BsiHKCI CYCGRG 1 cut(s) 777
BsiSI CCGG 2 cut(s) 519, 525
BslFI GGGAC 4 cut(s) 149, 812, 947, 1266
BslI CCNNNNNNNGG 2 cut(s) 377, 1136
BsmAI GTCTC 3 cut(s) 409, 440, 812
BsmBI CGTCTC 2 cut(s) 409, 440
BsmFI GGGAC 4 cut(s) 149, 812, 947, 1266
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 1 cut(s) 111
BsoBI CYCGRG 1 cut(s) 777
Bsp119I TTCGAA 1 cut(s) 980
Bsp1286I GDGCHC 2 cut(s) 518, 1073
Bsp143I GATC 3 cut(s) 79, 958, 1222
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 2 cut(s) 245, 419
BspDI ATCGAT 2 cut(s) 222, 243
BspFNI CGCG 2 cut(s) 198, 200
BspLI GGNNCC 5 cut(s) 382, 445, 517, 523, 831
BspMAI CTGCAG 2 cut(s) 492, 1230
BspPI GGATC 3 cut(s) 74, 966, 1230
BspT104I TTCGAA 1 cut(s) 980
BspT107I GGYRCC 2 cut(s) 380, 443
BsrDI GCAATG 1 cut(s) 1005
BsrI ACTGG 2 cut(s) 923, 935
BssECI CCNNGG 3 cut(s) 289, 582, 954
BssHII GCGCGC 1 cut(s) 198
BssMI GATC 3 cut(s) 79, 958, 1222
BssNAI GTATAC 1 cut(s) 540
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 954
Bst1107I GTATAC 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 108, 416
Bst4CI ACNGT 6 cut(s) 434, 544, 811, 912, 1257, 1348
Bst6I CTCTTC 2 cut(s) 149, 1080
BstACI GRCGYC 1 cut(s) 162
BstBAI YACGTR 1 cut(s) 1314
BstBI TTCGAA 1 cut(s) 980
BstC8I GCNNGC 2 cut(s) 200, 492
BstDEI CTNAG 2 cut(s) 232, 406
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 6 cut(s) 24, 123, 484, 928, 1001, 1192
BstFNI CGCG 2 cut(s) 198, 200
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 3 cut(s) 120, 200, 202
BstKTI GATC 3 cut(s) 82, 961, 1225
BstMAI GTCTC 3 cut(s) 409, 440, 812
BstMBI GATC 3 cut(s) 79, 958, 1222
BstMWI GCNNNNNNNGC 3 cut(s) 117, 387, 491
BstNI CCWGG 2 cut(s) 108, 416
BstSCI CCNGG 2 cut(s) 106, 414
BstSFI CTRYAG 5 cut(s) 385, 488, 855, 1082, 1226
BstSLI GKGCMC 1 cut(s) 1073
BstUI CGCG 2 cut(s) 198, 200
BstV1I GCAGC 1 cut(s) 1022
BstX2I RGATCY 2 cut(s) 958, 1222
BstYI RGATCY 2 cut(s) 958, 1222
BstZ17I GTATAC 1 cut(s) 540
Bsu15I ATCGAT 2 cut(s) 222, 243
BsuI GTATCC 1 cut(s) 991
BsuRI GGCC 1 cut(s) 111
BsuTUI ATCGAT 2 cut(s) 222, 243
BtgI CCRYGG 1 cut(s) 582
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 6 cut(s) 24, 123, 484, 928, 1001, 1192
BtsIMutI CAGTG 1 cut(s) 908
Cac8I GCNNGC 2 cut(s) 200, 492
CfoI GCGC 3 cut(s) 120, 200, 202
ClaI ATCGAT 2 cut(s) 222, 243
CseI GACGC 2 cut(s) 151, 391
Csp6I GTAC 3 cut(s) 951, 1052, 1315
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviAII CATG 7 cut(s) 91, 267, 707, 893, 1167, 1217, 1249
CviQI GTAC 3 cut(s) 951, 1052, 1315
DdeI CTNAG 2 cut(s) 232, 406
DpnI GATC 3 cut(s) 81, 960, 1224
DpnII GATC 3 cut(s) 79, 958, 1222
DraI TTTAAA 1 cut(s) 1279
DraIII CACNNNGTG 1 cut(s) 1042
Eam1104I CTCTTC 2 cut(s) 149, 1080
EarI CTCTTC 2 cut(s) 149, 1080
EciI GGCGGA 4 cut(s) 20, 543, 647, 1250
Eco130I CCWWGG 1 cut(s) 954
Eco147I AGGCCT 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 1290
Eco88I CYCGRG 1 cut(s) 777
EcoRI GAATTC 1 cut(s) 1177
EcoRII CCWGG 2 cut(s) 106, 414
EcoT14I CCWWGG 1 cut(s) 954
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 954
Esp3I CGTCTC 2 cut(s) 409, 440
FaeI CATG 7 cut(s) 94, 270, 710, 896, 1170, 1220, 1252
FaqI GGGAC 4 cut(s) 149, 812, 947, 1266
FatI CATG 7 cut(s) 90, 266, 706, 892, 1166, 1216, 1248
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 2 cut(s) 379, 1011
FokI GGATG 6 cut(s) 11, 110, 491, 935, 988, 1179
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 2 cut(s) 379, 1011
FspBI CTAG 2 cut(s) 1200, 1329
GlaI GCGC 3 cut(s) 119, 199, 201
GluI GCNGC 2 cut(s) 379, 1011
GsuI CTGGAG 1 cut(s) 54
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 1 cut(s) 111
HapII CCGG 2 cut(s) 519, 525
HgaI GACGC 2 cut(s) 151, 391
HhaI GCGC 3 cut(s) 120, 200, 202
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 7 cut(s) 94, 270, 710, 896, 1170, 1220, 1252
Hin6I GCGC 3 cut(s) 118, 198, 200
HinP1I GCGC 3 cut(s) 118, 198, 200
HpaII CCGG 2 cut(s) 519, 525
HphI GGTGA 3 cut(s) 332, 565, 586
Hpy166II GTNNAC 1 cut(s) 540
Hpy188I TCNGA 6 cut(s) 39, 424, 628, 807, 1183, 1285
Hpy188III TCNNGA 5 cut(s) 71, 83, 323, 779, 1091
Hpy8I GTNNAC 1 cut(s) 540
Hpy99I CGWCG 3 cut(s) 188, 443, 461
HpyAV CCTTC 6 cut(s) 160, 220, 682, 689, 1206, 1225
HpyCH4III ACNGT 6 cut(s) 434, 544, 811, 912, 1257, 1348
HpyCH4IV ACGT 1 cut(s) 1313
HpyCH4V TGCA 8 cut(s) 490, 770, 1010, 1036, 1061, 1170, 1216, 1228
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 387, 491
HpyF3I CTNAG 2 cut(s) 232, 406
HpySE526I ACGT 1 cut(s) 1313
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 7 cut(s) 94, 270, 710, 896, 1170, 1220, 1252
HspAI GCGC 3 cut(s) 118, 198, 200
Kzo9I GATC 3 cut(s) 79, 958, 1222
LmnI GCTCC 3 cut(s) 521, 527, 835
Lsp1109I GCAGC 1 cut(s) 1022
LweI GCATC 1 cut(s) 1201
MaeI CTAG 2 cut(s) 1200, 1329
MaeII ACGT 1 cut(s) 1313
MalI GATC 3 cut(s) 81, 960, 1224
MboI GATC 3 cut(s) 79, 958, 1222
MboII GAAGA 7 cut(s) 136, 277, 321, 329, 953, 1067, 1070
MflI RGATCY 2 cut(s) 958, 1222
MhlI GDGCHC 2 cut(s) 518, 1073
MluCI AATT 6 cut(s) 189, 353, 757, 976, 1177, 1209
MlyI GAGTC 4 cut(s) 293, 352, 413, 814
MroXI GAANNNNTTC 1 cut(s) 1177
MseI TTAA 3 cut(s) 170, 666, 1278
MslI CAYNNNNRTG 1 cut(s) 869
MspA1I CMGCKG 2 cut(s) 236, 1013
MspI CCGG 2 cut(s) 519, 525
MspR9I CCNGG 2 cut(s) 108, 416
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 2 cut(s) 108, 416
MvnI CGCG 2 cut(s) 198, 200
MwoI GCNNNNNNNGC 3 cut(s) 117, 387, 491
NdeII GATC 3 cut(s) 79, 958, 1222
NlaIII CATG 7 cut(s) 94, 270, 710, 896, 1170, 1220, 1252
NlaIV GGNNCC 5 cut(s) 382, 445, 517, 523, 831
NmeAIII GCCGAG 1 cut(s) 270
NspV TTCGAA 1 cut(s) 980
PaeR7I CTCGAG 1 cut(s) 777
PauI GCGCGC 1 cut(s) 198
PceI AGGCCT 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 1177
PfeI GAWTC 7 cut(s) 224, 245, 326, 425, 610, 703, 1156
PfoI TCCNGGA 1 cut(s) 414
PkrI GCNGC 2 cut(s) 380, 1012
PleI GAGTC 4 cut(s) 293, 352, 413, 814
PpsI GAGTC 4 cut(s) 293, 352, 413, 814
Ppu21I YACGTR 1 cut(s) 1314
Psp6I CCWGG 2 cut(s) 106, 414
PspGI CCWGG 2 cut(s) 106, 414
PspN4I GGNNCC 5 cut(s) 382, 445, 517, 523, 831
PstI CTGCAG 2 cut(s) 492, 1230
PsuI RGATCY 2 cut(s) 958, 1222
PteI GCGCGC 1 cut(s) 198
PvuII CAGCTG 2 cut(s) 236, 1013
RsaI GTAC 3 cut(s) 952, 1053, 1316
RsaNI GTAC 3 cut(s) 951, 1052, 1315
RseI CAYNNNNRTG 1 cut(s) 869
SaqAI TTAA 3 cut(s) 170, 666, 1278
SatI GCNGC 2 cut(s) 379, 1011
Sau3AI GATC 3 cut(s) 79, 958, 1222
SchI GAGTC 4 cut(s) 293, 352, 413, 814
ScrFI CCNGG 2 cut(s) 108, 416
SduI GDGCHC 2 cut(s) 518, 1073
SfaNI GCATC 1 cut(s) 1201
SfcI CTRYAG 5 cut(s) 385, 488, 855, 1082, 1226
Sfr274I CTCGAG 1 cut(s) 777
SfuI TTCGAA 1 cut(s) 980
SlaI CTCGAG 1 cut(s) 777
SmiMI CAYNNNNRTG 1 cut(s) 869
SmlI CTYRAG 3 cut(s) 45, 113, 777
SmoI CTYRAG 3 cut(s) 45, 113, 777
Sse9I AATT 6 cut(s) 189, 353, 757, 976, 1177, 1209
SseBI AGGCCT 1 cut(s) 111
SspMI CTAG 2 cut(s) 1200, 1329
StuI AGGCCT 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 106, 414
StyI CCWWGG 1 cut(s) 954
TaaI ACNGT 6 cut(s) 434, 544, 811, 912, 1257, 1348
TaiI ACGT 1 cut(s) 1316
TaqI TCGA 9 cut(s) 78, 186, 216, 222, 243, 297, 456, 778, 980
TaqII GACCGA 1 cut(s) 365
TasI AATT 6 cut(s) 189, 353, 757, 976, 1177, 1209
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 7 cut(s) 224, 245, 326, 425, 610, 703, 1156
Tru1I TTAA 3 cut(s) 170, 666, 1278
Tru9I TTAA 3 cut(s) 170, 666, 1278
TscAI CASTG 1 cut(s) 915
TseI GCWGC 1 cut(s) 1010
TspDTI ATGAA 5 cut(s) 75, 142, 695, 863, 1237
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 1 cut(s) 915
XapI RAATTY 3 cut(s) 189, 976, 1177
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XhoI CTCGAG 1 cut(s) 777
XmiI GTMKAC 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 1177
XspI CTAG 2 cut(s) 1200, 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.