pycom05g16530

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0021027
Physical Location & Seq
Reverse (-)
19782272 .. 19783842
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g16530.2

Sequence Viewer

Length: 672 bp
ATGCAGGCAGTAATAAAGAGAACAAATTTGAACCCAAATGAGGTGGGCGATATAGTTGTTGGTACAGTTATGGCACCTGGCTCACTTAGAGCAACTGAGCACAGGATGGCAGCTTTTTATGCTGGATTTCCAGATACGGTTCCCATCACAACTGTTAACAGGCAATGTTCATCTAGCCTCCAGGCAGTTGCTGATGTAGCTGCTGCTATAAAAGCAGGATATTATGACATCGGAAATGATAGCCAGGTGAGTGACGGTGCTGGAGCAGTCCTCCTTATGAAGAGAAGTTTAGCTATGCAGAAGGGACTTCCTATTCTTGGTGTTTTCAGGAGTTTTGCTGCTGTTGGTGTGGATCCCGCTGTCATGGGAGTGCTTCCAGCTGCTGCACTTCCGATAGCAGTGCAGTCTGCTGGCCTTGAGCTTGATGACATAGATCTATTTGAGATAAATGAGGCATTTGCATCTCAATATGTATATTGTGTCAAGAAATTGGAGCTTGATCCTGAGAAAGTCAATGTTAACGGAGGCACTATTGCTCTAGGGCATCCTTTAGGTGCTACAGGTTCTCACTGTGTTGCAACTCTCCTGGATGAGACGAAACGCCGTGGCAAGGATCACCGCTTTGGTGTGATCTCCATGTGCATAGGTATCCTTTTGCGCTCTTCCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

224

Amino Acids

23.43

Weight (kDa)

6.07

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021027)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19480
pyrus_communis pycom05g16530 pycom10g14520
rosa_multiflora Rmu_co8313601.1_g000001
rosa_roxburghii Rroxscaffold_7G00183500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AciI CCGC 2 cut(s) 357, 619
AclWI GGATC 4 cut(s) 347, 360, 494, 621
AcsI RAATTY 1 cut(s) 25
AfaI GTAC 1 cut(s) 64
AfiI CCNNNNNNNGG 3 cut(s) 40, 317, 610
AgsI TTSAA 1 cut(s) 31
AjnI CCWGG 4 cut(s) 76, 180, 243, 585
AluBI AGCT 6 cut(s) 113, 200, 293, 380, 421, 496
AluI AGCT 6 cut(s) 113, 200, 293, 380, 421, 496
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 587
AlwI GGATC 4 cut(s) 347, 360, 494, 621
AlwNI CAGNNNCTG 2 cut(s) 191, 383
AoxI GGCC 1 cut(s) 412
ApeKI GCWGC 6 cut(s) 110, 200, 203, 338, 380, 383
ApoI RAATTY 1 cut(s) 25
AspLEI GCGC 1 cut(s) 660
AsuHPI GGTGA 2 cut(s) 259, 608
BamHI GGATCC 1 cut(s) 352
BanI GGYRCC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 6 cut(s) 122, 187, 190, 325, 367, 370
BccI CCATC 2 cut(s) 100, 152
BceAI ACGGC 1 cut(s) 588
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BciT130I CCWGG 4 cut(s) 78, 182, 245, 587
BciVI GTATCC 1 cut(s) 659
BcoDI GTCTC 1 cut(s) 587
BfaI CTAG 2 cut(s) 174, 539
BfmI CTRYAG 1 cut(s) 558
BfuI GTATCC 1 cut(s) 659
BglII AGATCT 1 cut(s) 433
BisI GCNGC 6 cut(s) 111, 201, 204, 339, 381, 384
BlsI GCNGC 6 cut(s) 112, 202, 205, 340, 382, 385
Bme1390I CCNGG 4 cut(s) 78, 182, 245, 587
BmiI GGNNCC 3 cut(s) 75, 141, 354
BmrFI CCNGG 4 cut(s) 78, 182, 245, 587
BmsI GCATC 2 cut(s) 470, 553
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 2 cut(s) 164, 282
BpuEI CTTGAG 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 604
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 317, 610
Bse3DI GCAATG 1 cut(s) 170
BseBI CCWGG 4 cut(s) 78, 182, 245, 587
BseDI CCNNGG 1 cut(s) 604
BseGI GGATG 3 cut(s) 111, 544, 595
BseLI CCNNNNNNNGG 3 cut(s) 40, 317, 610
BseMI GCAATG 1 cut(s) 170
BseMII CTCAG 2 cut(s) 87, 495
BseXI GCAGC 6 cut(s) 122, 187, 190, 325, 367, 370
BsgI GTGCAG 2 cut(s) 369, 422
BshFI GGCC 1 cut(s) 414
BshNI GGYRCC 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 102
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 3 cut(s) 40, 317, 610
BsmAI GTCTC 1 cut(s) 587
BsmBI CGTCTC 1 cut(s) 587
BsmFI GGGAC 1 cut(s) 318
BsnI GGCC 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 5 cut(s) 352, 433, 499, 613, 630
BspACI CCGC 2 cut(s) 357, 619
BspANI GGCC 1 cut(s) 414
BspCNI CTCAG 2 cut(s) 88, 496
BspLI GGNNCC 3 cut(s) 75, 141, 354
BspPI GGATC 4 cut(s) 347, 360, 494, 621
BspQI GCTCTTC 1 cut(s) 667
BspT107I GGYRCC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 604
BssMI GATC 5 cut(s) 352, 433, 499, 613, 630
Bst2UI CCWGG 4 cut(s) 78, 182, 245, 587
Bst4CI ACNGT 5 cut(s) 67, 139, 154, 257, 572
Bst6I CTCTTC 2 cut(s) 275, 667
BstC8I GCNNGC 2 cut(s) 6, 412
BstDEI CTNAG 3 cut(s) 86, 96, 504
BstDSI CCRYGG 1 cut(s) 604
BstF5I GGATG 3 cut(s) 111, 544, 595
BstHHI GCGC 1 cut(s) 660
BstKTI GATC 5 cut(s) 355, 436, 502, 616, 633
BstMAI GTCTC 1 cut(s) 587
BstMBI GATC 5 cut(s) 352, 433, 499, 613, 630
BstMWI GCNNNNNNNGC 3 cut(s) 119, 197, 212
BstNI CCWGG 4 cut(s) 78, 182, 245, 587
BstSCI CCNGG 4 cut(s) 76, 180, 243, 585
BstSFI CTRYAG 1 cut(s) 558
BstV1I GCAGC 6 cut(s) 122, 187, 190, 325, 367, 370
BstX2I RGATCY 2 cut(s) 352, 433
BstYI RGATCY 2 cut(s) 352, 433
BsuI GTATCC 1 cut(s) 659
BsuRI GGCC 1 cut(s) 414
BtgI CCRYGG 1 cut(s) 604
BtsCI GGATG 3 cut(s) 111, 544, 595
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 405, 568
Cac8I GCNNGC 2 cut(s) 6, 412
CaiI CAGNNNCTG 2 cut(s) 191, 383
CfoI GCGC 1 cut(s) 660
Csp6I GTAC 1 cut(s) 63
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviAII CATG 2 cut(s) 364, 637
CviQI GTAC 1 cut(s) 63
DdeI CTNAG 3 cut(s) 86, 96, 504
DpnI GATC 5 cut(s) 354, 435, 501, 615, 632
DpnII GATC 5 cut(s) 352, 433, 499, 613, 630
Eam1104I CTCTTC 2 cut(s) 275, 667
EarI CTCTTC 2 cut(s) 275, 667
EcoRII CCWGG 4 cut(s) 76, 180, 243, 585
Esp3I CGTCTC 1 cut(s) 587
FaeI CATG 2 cut(s) 367, 640
FaqI GGGAC 1 cut(s) 318
FatI CATG 2 cut(s) 363, 636
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 6 cut(s) 111, 201, 204, 339, 381, 384
FokI GGATG 3 cut(s) 118, 531, 602
Fsp4HI GCNGC 6 cut(s) 111, 201, 204, 339, 381, 384
FspBI CTAG 2 cut(s) 174, 539
GlaI GCGC 1 cut(s) 659
GluI GCNGC 6 cut(s) 111, 201, 204, 339, 381, 384
GsuI CTGGAG 2 cut(s) 164, 282
HaeIII GGCC 1 cut(s) 414
HhaI GCGC 1 cut(s) 660
Hin1II CATG 2 cut(s) 367, 640
Hin6I GCGC 1 cut(s) 658
HinP1I GCGC 1 cut(s) 658
HincII GTYRAC 2 cut(s) 157, 520
HindII GTYRAC 2 cut(s) 157, 520
HpaI GTTAAC 2 cut(s) 157, 520
HphI GGTGA 2 cut(s) 259, 608
Hpy166II GTNNAC 2 cut(s) 157, 520
Hpy188I TCNGA 2 cut(s) 233, 393
Hpy188III TCNNGA 4 cut(s) 131, 328, 484, 503
Hpy8I GTNNAC 2 cut(s) 157, 520
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 5 cut(s) 67, 139, 154, 257, 572
HpyCH4V TGCA 7 cut(s) 4, 298, 386, 403, 461, 578, 642
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 197, 212
HpyF3I CTNAG 3 cut(s) 86, 96, 504
Hsp92II CATG 2 cut(s) 367, 640
HspAI GCGC 1 cut(s) 658
KspAI GTTAAC 2 cut(s) 157, 520
Kzo9I GATC 5 cut(s) 352, 433, 499, 613, 630
LguI GCTCTTC 1 cut(s) 667
LmnI GCTCC 2 cut(s) 263, 493
Lsp1109I GCAGC 6 cut(s) 122, 187, 190, 325, 367, 370
LweI GCATC 2 cut(s) 470, 553
MaeI CTAG 2 cut(s) 174, 539
MaeIII GTNAC 1 cut(s) 251
MalI GATC 5 cut(s) 354, 435, 501, 615, 632
MboI GATC 5 cut(s) 352, 433, 499, 613, 630
MboII GAAGA 2 cut(s) 292, 654
MflI RGATCY 2 cut(s) 352, 433
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 2 cut(s) 25, 488
MnlI CCTC 5 cut(s) 34, 188, 281, 445, 518
MseI TTAA 2 cut(s) 156, 519
MslI CAYNNNNRTG 1 cut(s) 368
MspA1I CMGCKG 2 cut(s) 359, 380
MspR9I CCNGG 4 cut(s) 78, 182, 245, 587
MvaI CCWGG 4 cut(s) 78, 182, 245, 587
MwoI GCNNNNNNNGC 3 cut(s) 119, 197, 212
NdeII GATC 5 cut(s) 352, 433, 499, 613, 630
NlaIII CATG 2 cut(s) 367, 640
NlaIV GGNNCC 3 cut(s) 75, 141, 354
NmuCI GTSAC 1 cut(s) 251
PciSI GCTCTTC 1 cut(s) 667
PfoI TCCNGGA 1 cut(s) 585
PkrI GCNGC 6 cut(s) 112, 202, 205, 340, 382, 385
Psp6I CCWGG 4 cut(s) 76, 180, 243, 585
PspGI CCWGG 4 cut(s) 76, 180, 243, 585
PspN4I GGNNCC 3 cut(s) 75, 141, 354
PstNI CAGNNNCTG 2 cut(s) 191, 383
PsuI RGATCY 2 cut(s) 352, 433
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
RseI CAYNNNNRTG 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 667
SaqAI TTAA 2 cut(s) 156, 519
SatI GCNGC 6 cut(s) 111, 201, 204, 339, 381, 384
Sau3AI GATC 5 cut(s) 352, 433, 499, 613, 630
ScrFI CCNGG 4 cut(s) 78, 182, 245, 587
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 2 cut(s) 470, 553
SfcI CTRYAG 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 2 cut(s) 25, 488
SsiI CCGC 2 cut(s) 357, 619
SspMI CTAG 2 cut(s) 174, 539
StyD4I CCNGG 4 cut(s) 76, 180, 243, 585
TaaI ACNGT 5 cut(s) 67, 139, 154, 257, 572
TasI AATT 2 cut(s) 25, 488
Tru1I TTAA 2 cut(s) 156, 519
Tru9I TTAA 2 cut(s) 156, 519
TscAI CASTG 2 cut(s) 405, 575
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 6 cut(s) 110, 200, 203, 338, 380, 383
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 2 cut(s) 159, 293
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 2 cut(s) 405, 575
XapI RAATTY 1 cut(s) 25
XspI CTAG 2 cut(s) 174, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.