pycom05g23670

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018512
Physical Location & Seq
Reverse (-)
25478366 .. 25478893
528 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 339 bp
ATGGTGAAGGTCAAGGAAAAGGATGCCAAGTCGAAGTGCCTCAAGTTTGATATTGAAAAATTTCATCATCACAAGGACCATGACCATGACCATCATGATGATTATTGCTTGGAGATTACCCAAGCAGTTACCTTTTTGCCTACCATCGCTGATCATCATATGTTGTTCGAAAACATTGCAAGGAAGGTGGTTTTGTCGCCCTTGAGAAAAACTATGGCGCCAGAGGCCTGGGAACCTGCCAACCGGTGGGGTTATAGAGCGGAGTACAAGCGAGAGCAACTGGCTGTAGCTAATGAGGTTGATCCTGGCGTGCAGCGCTGCTTTTGCATGACATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

113

Amino Acids

13.25

Weight (kDa)

6.74

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 246
AccBSI CCGCTC 1 cut(s) 260
AciI CCGC 1 cut(s) 260
AclWI GGATC 1 cut(s) 296
AcsI RAATTY 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 1 cut(s) 266
AfeI AGCGCT 1 cut(s) 317
AfiI CCNNNNNNNGG 1 cut(s) 246
AgeI ACCGGT 1 cut(s) 243
AgsI TTSAA 1 cut(s) 56
AjnI CCWGG 2 cut(s) 227, 304
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
AlwI GGATC 1 cut(s) 296
Aor51HI AGCGCT 1 cut(s) 317
AoxI GGCC 1 cut(s) 225
ApeKI GCWGC 2 cut(s) 313, 318
ApoI RAATTY 1 cut(s) 59
AsiGI ACCGGT 1 cut(s) 243
Asp700I GAANNNNTTC 1 cut(s) 60
AspLEI GCGC 2 cut(s) 220, 318
AspS9I GGNCC 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 168
AvaII GGWCC 1 cut(s) 76
BanI GGYRCC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 305, 325
BccI CCATC 2 cut(s) 99, 152
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 2 cut(s) 229, 306
BclI TGATCA 1 cut(s) 151
BfmI CTRYAG 1 cut(s) 285
BfoI RGCGCY 2 cut(s) 221, 319
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 2 cut(s) 314, 319
BlsI GCNGC 2 cut(s) 315, 320
Bme1390I CCNGG 2 cut(s) 229, 306
Bme18I GGWCC 1 cut(s) 76
BmgT120I GGNCC 1 cut(s) 76
BmiI GGNNCC 2 cut(s) 219, 234
BmrFI CCNGG 2 cut(s) 229, 306
BmsI GCATC 1 cut(s) 13
Bpu14I TTCGAA 1 cut(s) 168
BpuEI CTTGAG 2 cut(s) 26, 223
BsaHI GRCGYC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 246
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 2 cut(s) 229, 306
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 1 cut(s) 246
BseMI GCAATG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 305, 325
BsgI GTGCAG 1 cut(s) 332
BshFI GGCC 1 cut(s) 227
BshNI GGYRCC 1 cut(s) 217
BshTI ACCGGT 1 cut(s) 243
BsiSI CCGG 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 246
BsnI GGCC 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 168
Bsp143I GATC 2 cut(s) 151, 301
BspACI CCGC 1 cut(s) 260
BspANI GGCC 1 cut(s) 227
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 219, 234
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 1 cut(s) 296
BspT104I TTCGAA 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 217
BsrBI CCGCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 285
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 2 cut(s) 151, 301
BssNI GRCGYC 1 cut(s) 218
Bst2UI CCWGG 2 cut(s) 229, 306
BstACI GRCGYC 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 311
BstF5I GGATG 1 cut(s) 28
BstH2I RGCGCY 2 cut(s) 221, 319
BstHHI GCGC 2 cut(s) 220, 318
BstKTI GATC 2 cut(s) 154, 304
BstMBI GATC 2 cut(s) 151, 301
BstMWI GCNNNNNNNGC 3 cut(s) 224, 315, 324
BstNI CCWGG 2 cut(s) 229, 306
BstSCI CCNGG 2 cut(s) 227, 304
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 2 cut(s) 305, 325
BstXI CCANNNNNNTGG 1 cut(s) 228
BsuRI GGCC 1 cut(s) 227
BtgZI GCGATG 1 cut(s) 130
BtsCI GGATG 1 cut(s) 28
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 311
CciI TCATGA 1 cut(s) 94
CfoI GCGC 2 cut(s) 220, 318
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 265
CspAI ACCGGT 1 cut(s) 243
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviAII CATG 4 cut(s) 80, 86, 95, 328
CviJI RGCY 3 cut(s) 227, 284, 290
CviKI_1 RGCY 3 cut(s) 227, 284, 290
CviQI GTAC 1 cut(s) 265
DinI GGCGCC 1 cut(s) 219
DpnI GATC 2 cut(s) 153, 303
DpnII GATC 2 cut(s) 151, 301
Eco147I AGGCCT 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 76
Eco47III AGCGCT 1 cut(s) 317
EcoRII CCWGG 2 cut(s) 227, 304
EgeI GGCGCC 1 cut(s) 219
EheI GGCGCC 1 cut(s) 219
FaeI CATG 4 cut(s) 83, 89, 98, 331
FaiI YATR 9 cut(s) 81, 87, 96, 159, 161, 215, 255, 329, 334
FatI CATG 4 cut(s) 79, 85, 94, 327
FauNDI CATATG 1 cut(s) 159
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 2 cut(s) 314, 319
FokI GGATG 1 cut(s) 35
Fsp4HI GCNGC 2 cut(s) 314, 319
GlaI GCGC 2 cut(s) 219, 317
GluI GCNGC 2 cut(s) 314, 319
HaeII RGCGCY 2 cut(s) 221, 319
HaeIII GGCC 1 cut(s) 227
HapII CCGG 1 cut(s) 244
HhaI GCGC 2 cut(s) 220, 318
Hin1I GRCGYC 1 cut(s) 218
Hin1II CATG 4 cut(s) 83, 89, 98, 331
Hin6I GCGC 2 cut(s) 218, 316
HinP1I GCGC 2 cut(s) 218, 316
HpaII CCGG 1 cut(s) 244
HphI GGTGA 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 178
HpyCH4V TGCA 3 cut(s) 179, 313, 327
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 315, 324
Hsp92I GRCGYC 1 cut(s) 218
Hsp92II CATG 4 cut(s) 83, 89, 98, 331
HspAI GCGC 2 cut(s) 218, 316
KasI GGCGCC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 2 cut(s) 151, 301
LpnPI CCDG 8 cut(s) 214, 234, 241, 249, 257, 266, 291, 318
Lsp1109I GCAGC 2 cut(s) 305, 325
LweI GCATC 1 cut(s) 13
MaeIII GTNAC 1 cut(s) 127
MalI GATC 2 cut(s) 153, 303
MbiI CCGCTC 1 cut(s) 260
MboI GATC 2 cut(s) 151, 301
MluCI AATT 1 cut(s) 59
Mly113I GGCGCC 1 cut(s) 218
MnlI CCTC 3 cut(s) 50, 217, 289
MroXI GAANNNNTTC 1 cut(s) 60
MseI TTAA 1 cut(s) 337
MslI CAYNNNNRTG 2 cut(s) 84, 96
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 2 cut(s) 229, 306
MvaI CCWGG 2 cut(s) 229, 306
MwoI GCNNNNNNNGC 3 cut(s) 224, 315, 324
NarI GGCGCC 1 cut(s) 218
NdeI CATATG 1 cut(s) 159
NdeII GATC 2 cut(s) 151, 301
NlaIII CATG 4 cut(s) 83, 89, 98, 331
NlaIV GGNNCC 2 cut(s) 219, 234
NspV TTCGAA 1 cut(s) 168
PagI TCATGA 1 cut(s) 94
PceI AGGCCT 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 60
PflMI CCANNNNNTGG 1 cut(s) 246
PinAI ACCGGT 1 cut(s) 243
PkrI GCNGC 2 cut(s) 315, 320
PluTI GGCGCC 1 cut(s) 221
Psp6I CCWGG 2 cut(s) 227, 304
PspGI CCWGG 2 cut(s) 227, 304
PspN4I GGNNCC 2 cut(s) 219, 234
PspPI GGNCC 1 cut(s) 76
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 2 cut(s) 84, 96
SaqAI TTAA 1 cut(s) 337
SatI GCNGC 2 cut(s) 314, 319
Sau3AI GATC 2 cut(s) 151, 301
Sau96I GGNCC 1 cut(s) 76
ScrFI CCNGG 2 cut(s) 229, 306
SetI ASST 6 cut(s) 12, 134, 189, 238, 292, 300
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 285
SfoI GGCGCC 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 168
SinI GGWCC 1 cut(s) 76
SmiMI CAYNNNNRTG 2 cut(s) 84, 96
SmlI CTYRAG 2 cut(s) 41, 202
SmoI CTYRAG 2 cut(s) 41, 202
Sse9I AATT 1 cut(s) 59
SseBI AGGCCT 1 cut(s) 227
SsiI CCGC 1 cut(s) 260
SspDI GGCGCC 1 cut(s) 217
StuI AGGCCT 1 cut(s) 227
StyD4I CCNGG 2 cut(s) 227, 304
TaqI TCGA 2 cut(s) 32, 168
TasI AATT 1 cut(s) 59
TatI WGTACW 1 cut(s) 264
Tru1I TTAA 1 cut(s) 337
Tru9I TTAA 1 cut(s) 337
TseI GCWGC 2 cut(s) 313, 318
TspDTI ATGAA 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.