pycom05g23710

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018512
Physical Location & Seq
Reverse (-)
25510303 .. 25510749
447 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
ATGTACTTCAATTGCACCAAGGATGCCATGTCGACCATGGAATTCCACAAAACTCTGATGAACCAAGTGTTTCCCTCGGCCTGGTCGCATAATCCTCTAACCACCCATAAACTCATCTGCAACTTCATAGACAGACGCGACGACGGTGGAAAACGCAACCAAGATGAAGCTTTCTGCACTGCGGCGGTTTGGCTCCACCAGAACCACTCGAAAACCCTTGCATACAATCTTGTGCTAATTTCCGGCTCCTTTGGACGTATCCTACACTTTCCCTGCATTCTCTTGCAGGTTTTACGGGGCAAAGCTCACTTGAAATTTACTGGGAGGACGAGGGAAGAGGCAGATTCAAACGGAATGGTGAAGGTCAAGGAAAAGGATGCCAAGTCGAAGGTAAGTGAATTGTTGTTGAGGAGGAAGGTGATCGACACGGCCAACGAGGCGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

149

Amino Acids

16.87

Weight (kDa)

9.41

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 16 - 81 3.4e-13 Domain of unknown function (DUF2828)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccI GTMKAC 1 cut(s) 32
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 182, 185, 440
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 2 cut(s) 41, 314
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 81
AgsI TTSAA 3 cut(s) 10, 313, 348
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 2 cut(s) 170, 305
AluI AGCT 2 cut(s) 170, 305
AoxI GGCC 2 cut(s) 78, 429
ApoI RAATTY 2 cut(s) 41, 314
AsuHPI GGTGA 2 cut(s) 370, 430
BceAI ACGGC 1 cut(s) 444
BciT130I CCWGG 1 cut(s) 82
BciVI GTATCC 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 277
BfuI GTATCC 1 cut(s) 269
BglI GCCNNNNNGGC 1 cut(s) 437
BisI GCNGC 2 cut(s) 183, 441
BlsI GCNGC 2 cut(s) 184, 442
Bme1390I CCNGG 1 cut(s) 82
BmiI GGNNCC 2 cut(s) 194, 247
BmrFI CCNGG 1 cut(s) 82
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 2 cut(s) 13, 367
BmuI ACTGGG 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 18, 36, 75
Bsc4I CCNNNNNNNGG 1 cut(s) 81
Bse1I ACTGG 1 cut(s) 325
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 3 cut(s) 18, 36, 75
BseGI GGATG 2 cut(s) 28, 382
BseLI CCNNNNNNNGG 1 cut(s) 81
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 1 cut(s) 424
BsgI GTGCAG 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 2 cut(s) 80, 431
BsiSI CCGG 1 cut(s) 243
BslI CCNNNNNNNGG 1 cut(s) 81
BsmI GAATGC 1 cut(s) 276
BsnI GGCC 2 cut(s) 80, 431
Bsp143I GATC 1 cut(s) 420
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 3 cut(s) 182, 185, 440
BspANI GGCC 2 cut(s) 80, 431
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 194, 247
BspMI ACCTGC 1 cut(s) 277
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 3 cut(s) 18, 36, 75
BssMI GATC 1 cut(s) 420
BssT1I CCWWGG 2 cut(s) 18, 36
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 444
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 2 cut(s) 28, 382
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 1 cut(s) 423
BstMBI GATC 1 cut(s) 420
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 80
BstUI CGCG 1 cut(s) 138
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 2 cut(s) 80, 431
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 28, 382
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 177
BveI ACCTGC 1 cut(s) 277
CseI GACGC 1 cut(s) 144
Csp6I GTAC 1 cut(s) 4
CviAII CATG 2 cut(s) 28, 37
CviJI RGCY 7 cut(s) 80, 170, 193, 246, 305, 431, 443
CviKI_1 RGCY 7 cut(s) 80, 170, 193, 246, 305, 431, 443
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 444
DpnI GATC 1 cut(s) 422
DpnII GATC 1 cut(s) 420
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 2 cut(s) 18, 36
EcoRI GAATTC 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 2 cut(s) 18, 36
ErhI CCWWGG 2 cut(s) 18, 36
FaeI CATG 2 cut(s) 31, 40
FaiI YATR 6 cut(s) 29, 38, 90, 108, 128, 223
FatI CATG 2 cut(s) 27, 36
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 2 cut(s) 183, 441
FokI GGATG 2 cut(s) 35, 389
Fsp4HI GCNGC 2 cut(s) 183, 441
GluI GCNGC 2 cut(s) 183, 441
HaeIII GGCC 2 cut(s) 80, 431
HapII CCGG 1 cut(s) 243
HgaI GACGC 1 cut(s) 144
Hin1II CATG 2 cut(s) 31, 40
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 168
HinfI GANTC 1 cut(s) 344
HpaII CCGG 1 cut(s) 243
HphI GGTGA 2 cut(s) 370, 430
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 1 cut(s) 57
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 2 cut(s) 143, 146
HpyAV CCTTC 3 cut(s) 355, 382, 409
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 6 cut(s) 15, 120, 177, 221, 276, 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 2 cut(s) 31, 40
Kzo9I GATC 1 cut(s) 420
LmnI GCTCC 2 cut(s) 198, 251
LpnPI CCDG 7 cut(s) 67, 94, 212, 256, 272, 286, 306
LweI GCATC 2 cut(s) 13, 367
MaeII ACGT 1 cut(s) 256
MalI GATC 1 cut(s) 422
MboI GATC 1 cut(s) 420
MboII GAAGA 1 cut(s) 347
MfeI CAATTG 1 cut(s) 10
MluCI AATT 5 cut(s) 10, 41, 237, 314, 398
MnlI CCTC 8 cut(s) 85, 105, 318, 324, 331, 402, 405, 430
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 82
MunI CAATTG 1 cut(s) 10
Mva1269I GAATGC 1 cut(s) 276
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 437
NcoI CCATGG 1 cut(s) 36
NdeII GATC 1 cut(s) 420
NlaIII CATG 2 cut(s) 31, 40
NlaIV GGNNCC 2 cut(s) 194, 247
NmeAIII GCCGAG 1 cut(s) 56
PctI GAATGC 1 cut(s) 276
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 2 cut(s) 184, 442
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 194, 247
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SalI GTCGAC 1 cut(s) 31
SatI GCNGC 2 cut(s) 183, 441
Sau3AI GATC 1 cut(s) 420
ScrFI CCNGG 1 cut(s) 82
SetI ASST 7 cut(s) 172, 259, 291, 307, 366, 393, 420
SfaNI GCATC 2 cut(s) 13, 367
Sse9I AATT 5 cut(s) 10, 41, 237, 314, 398
SsiI CCGC 3 cut(s) 182, 185, 440
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 2 cut(s) 18, 36
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 1 cut(s) 259
TaqI TCGA 4 cut(s) 32, 209, 386, 423
TasI AATT 5 cut(s) 10, 41, 237, 314, 398
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 185, 443
TfiI GAWTC 1 cut(s) 344
TscAI CASTG 1 cut(s) 184
TspDTI ATGAA 3 cut(s) 74, 115, 180
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 184
XapI RAATTY 2 cut(s) 41, 314
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.