pycom05g24130

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018512
Physical Location & Seq
Reverse (-)
25693787 .. 25694473
687 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGTACTTCCATTGCACCAAGGACGCCATGTCGATCGTGGAATTCCACAGAACTCTGATGAACCAAGTGCTTCCCTTGGCCTGGTTGCATAATCCTCTAACCACCCACAAACTCATCTGCAACTTCATAGACAGACGCGGCGACGGTGAAAAACGCAACCAAGACGAAGCTTTCTTCACAGCGGCGGTTTGGCTCCACCAGAACCACTTGAAAACCTTTGCATACAATCTTGTGCCAATTTCCGGCTCCTTTGGACATATCCTACACCTTCCCTGCATTCTTTCGCAGGTTTTACGGGGCGAAGCTCACTTGAAATTTACTGGAAGGACGAGGGAAGAGGCGGATTCAAACGGAATGGTGAAGGTCAAGGAAAAGGATGCCAAGTCAAAGGTAAGCGAATTGTTGTTGAGGAGGAAGGTGATCGACATGGCCAACAAGGTGGCTCAGAAATACAAGCGCGACATTGATTATTGCTTCTTGCACAACTGTGTTTTGGATATTTTTGCCCAGAGCCTCAAGTTTGATATTGAAAAATTTCATCATCACAAGGACCATGACCATGACCATCATGATGATTATTGCTTGGAGATTACCCAAGCAGTTACCTTTTTGCCTACCATCGCTGATCATCATATGTTGTTTGAAAACATTGCGAGGAAGGTTTTCCGGTCAGAATCATGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

229

Amino Acids

26.66

Weight (kDa)

7.74

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 18 - 225 2.7e-20 Domain of unknown function (DUF2828)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccII CGCG 2 cut(s) 138, 459
AciI CCGC 4 cut(s) 138, 182, 185, 341
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 3 cut(s) 41, 314, 533
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 81, 242
AgsI TTSAA 5 cut(s) 211, 313, 348, 530, 644
AhdI GACNNNNNGTC 1 cut(s) 28
AjnI CCWGG 1 cut(s) 80
AleI CACNNNNGTG 1 cut(s) 486
AluBI AGCT 2 cut(s) 170, 305
AluI AGCT 2 cut(s) 170, 305
AoxI GGCC 2 cut(s) 78, 429
ApoI RAATTY 3 cut(s) 41, 314, 533
Asp700I GAANNNNTTC 2 cut(s) 534, 662
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 550
AsuHPI GGTGA 3 cut(s) 158, 370, 430
AvaII GGWCC 1 cut(s) 550
BalI TGGCCA 1 cut(s) 431
BccI CCATC 2 cut(s) 573, 626
BciT130I CCWGG 1 cut(s) 82
BclI TGATCA 1 cut(s) 625
BfuAI ACCTGC 1 cut(s) 277
BisI GCNGC 2 cut(s) 139, 183
BlsI GCNGC 2 cut(s) 140, 184
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 550
BmeRI GACNNNNNGTC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 550
BmiI GGNNCC 2 cut(s) 194, 247
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 500
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 18, 75
BsaWI WCCGGW 1 cut(s) 666
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 242
Bse1I ACTGG 1 cut(s) 325
Bse3DI GCAATG 2 cut(s) 10, 648
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 2 cut(s) 18, 75
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 2 cut(s) 81, 242
BseMI GCAATG 2 cut(s) 10, 648
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 1 cut(s) 424
Bsh1236I CGCG 2 cut(s) 138, 459
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 80, 431
BsiEI CGRYCG 1 cut(s) 36
BsiSI CCGG 2 cut(s) 243, 667
BslI CCNNNNNNNGG 2 cut(s) 81, 242
BsmI GAATGC 1 cut(s) 276
BsnI GGCC 2 cut(s) 80, 431
Bsp143I GATC 3 cut(s) 33, 420, 625
BspACI CCGC 4 cut(s) 138, 182, 185, 341
BspANI GGCC 2 cut(s) 80, 431
BspCNI CTCAG 1 cut(s) 457
BspFNI CGCG 2 cut(s) 138, 459
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 2 cut(s) 194, 247
BspMI ACCTGC 1 cut(s) 277
BsrDI GCAATG 2 cut(s) 10, 648
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 2 cut(s) 18, 75
BssMI GATC 3 cut(s) 33, 420, 625
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 2 cut(s) 18, 75
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 2 cut(s) 146, 488
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 24
BstDEI CTNAG 1 cut(s) 444
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 2 cut(s) 138, 459
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 3 cut(s) 36, 423, 628
BstMBI GATC 3 cut(s) 33, 420, 625
BstMCI CGRYCG 1 cut(s) 36
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 80
BstUI CGCG 2 cut(s) 138, 459
BstXI CCANNNNNNTGG 1 cut(s) 439
BsuRI GGCC 2 cut(s) 80, 431
BtgZI GCGATG 1 cut(s) 604
BtsCI GGATG 1 cut(s) 382
BveI ACCTGC 1 cut(s) 277
CciI TCATGA 1 cut(s) 568
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 550
CseI GACGC 2 cut(s) 32, 144
Csp6I GTAC 1 cut(s) 4
CviAII CATG 6 cut(s) 28, 427, 554, 560, 569, 678
CviJI RGCY 8 cut(s) 80, 170, 193, 246, 305, 431, 443, 513
CviKI_1 RGCY 8 cut(s) 80, 170, 193, 246, 305, 431, 443, 513
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 444
DpnI GATC 3 cut(s) 35, 422, 627
DpnII GATC 3 cut(s) 33, 420, 625
DriI GACNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 1 cut(s) 330
Eam1105I GACNNNNNGTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 18, 75
Eco47I GGWCC 1 cut(s) 550
EcoRI GAATTC 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 2 cut(s) 18, 75
ErhI CCWWGG 2 cut(s) 18, 75
FaeI CATG 6 cut(s) 31, 430, 557, 563, 572, 681
FatI CATG 6 cut(s) 27, 426, 553, 559, 568, 677
FauNDI CATATG 1 cut(s) 633
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 2 cut(s) 139, 183
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 2 cut(s) 139, 183
GlaI GCGC 1 cut(s) 458
GluI GCNGC 2 cut(s) 139, 183
HaeIII GGCC 2 cut(s) 80, 431
HapII CCGG 2 cut(s) 243, 667
HgaI GACGC 2 cut(s) 32, 144
HhaI GCGC 1 cut(s) 459
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 6 cut(s) 31, 430, 557, 563, 572, 681
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 168
HinfI GANTC 2 cut(s) 344, 674
HpaII CCGG 2 cut(s) 243, 667
HphI GGTGA 3 cut(s) 158, 370, 430
Hpy188I TCNGA 4 cut(s) 57, 447, 673, 686
Hpy188III TCNNGA 1 cut(s) 569
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 5 cut(s) 278, 318, 355, 409, 652
HpyCH4III ACNGT 2 cut(s) 146, 488
HpyCH4V TGCA 6 cut(s) 15, 88, 120, 221, 276, 481
HpyF3I CTNAG 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 6 cut(s) 31, 430, 557, 563, 572, 681
HspAI GCGC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 3 cut(s) 33, 420, 625
LmnI GCTCC 2 cut(s) 198, 251
LpnPI CCDG 9 cut(s) 67, 94, 212, 256, 272, 286, 306, 521, 680
LweI GCATC 1 cut(s) 367
MaeIII GTNAC 1 cut(s) 601
MalI GATC 3 cut(s) 35, 422, 627
MboI GATC 3 cut(s) 33, 420, 625
MboII GAAGA 2 cut(s) 166, 347
MlsI TGGCCA 1 cut(s) 431
MluCI AATT 5 cut(s) 41, 237, 314, 398, 533
MluNI TGGCCA 1 cut(s) 431
MnlI CCTC 7 cut(s) 105, 324, 331, 402, 405, 524, 648
Mox20I TGGCCA 1 cut(s) 431
MroXI GAANNNNTTC 2 cut(s) 534, 662
MscI TGGCCA 1 cut(s) 431
MslI CAYNNNNRTG 3 cut(s) 486, 558, 570
Msp20I TGGCCA 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 2 cut(s) 243, 667
MspR9I CCNGG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 276
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 2 cut(s) 138, 459
NdeI CATATG 1 cut(s) 633
NdeII GATC 3 cut(s) 33, 420, 625
NlaIII CATG 6 cut(s) 31, 430, 557, 563, 572, 681
NlaIV GGNNCC 2 cut(s) 194, 247
OliI CACNNNNGTG 1 cut(s) 486
PagI TCATGA 1 cut(s) 568
PctI GAATGC 1 cut(s) 276
PdmI GAANNNNTTC 2 cut(s) 534, 662
PfeI GAWTC 2 cut(s) 344, 674
PkrI GCNGC 2 cut(s) 140, 184
Ple19I CGATCG 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 194, 247
PspPI GGNCC 1 cut(s) 550
PvuI CGATCG 1 cut(s) 36
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 3 cut(s) 486, 558, 570
SatI GCNGC 2 cut(s) 139, 183
Sau3AI GATC 3 cut(s) 33, 420, 625
Sau96I GGNCC 1 cut(s) 550
ScrFI CCNGG 1 cut(s) 82
SfaNI GCATC 1 cut(s) 367
SinI GGWCC 1 cut(s) 550
SmiMI CAYNNNNRTG 3 cut(s) 486, 558, 570
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 5 cut(s) 41, 237, 314, 398, 533
SsiI CCGC 4 cut(s) 138, 182, 185, 341
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 2 cut(s) 18, 75
TaaI ACNGT 2 cut(s) 146, 488
TaqI TCGA 2 cut(s) 32, 423
TasI AATT 5 cut(s) 41, 237, 314, 398, 533
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 141, 185
TfiI GAWTC 2 cut(s) 344, 674
TspDTI ATGAA 3 cut(s) 74, 115, 527
TspGWI ACGGA 1 cut(s) 366
VpaK11BI GGWCC 1 cut(s) 550
XapI RAATTY 3 cut(s) 41, 314, 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XmnI GAANNNNTTC 2 cut(s) 534, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.