pycom06g06270

14 kDa proline-rich protein DC2.15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0017044
Physical Location & Seq
Reverse (-)
9745378 .. 9745800
423 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
ATGGCTTCTAAGGCTGTAGCTTCCACTGCCCTCCTCCTCTCCCTCAACCTCCTCTTCTTCACCTTGGTCACCTCCACCAATGTTGACTGCCCACCACCTGCAAAAAAGGGACACCACCCTAAGCACGCAAGTCCTGCACTACCAAACCCCAAGCCCTCTAAGCGTGCCACGTGCCCAGTAGACACTTTGAAATTGGGAGTATGTGCTGACGTGTTGAATGGTTTGGTGCACCTTGTCGTTGGTCCGCCAAAGTTCCCATGCTGCAGCCTCATTGAGGGCCTTGTTGACCTTGATGCTGCTGTGTGCCTTTGCACTGCAATCAAAGCTAATGTCTTGGGGATCCACTTAAACGTCCCCGTTTCACTGAGCCTGCTCTTAAACTACTGCGGGAAGCAGGTCCCTACTGGCTACGAATGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

141

Amino Acids

14.56

Weight (kDa)

8.6

Isoelectric Point (pI)

32.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 52 - 139 3.2e-06 Probable lipid transfer
Hydrophob_seed PF14547 57 - 140 3.1e-25 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 58 - 139 2.5e-16 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45180
fragaria_vesca FvH4_5g00110
prunus_persica Prupe.5G072800_v2.0.a1
pyrus_communis pycom06g06270
rosa_chinensis RchiOBHm_Chr7g0202821
rosa_laevigata RLG00000003569
rosa_multiflora Rmu_sc0011770.1_g000001
rosa_rugosa Rorug07G0073300
rosa_samantha Rh7AG201100 Rh7BG201300 Rh7CG212500 Rh7DG208000
rosa_wichuraiana Rw7G017590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 106
Acc36I ACCTGC 2 cut(s) 106, 385
AccI GTMKAC 1 cut(s) 180
AciI CCGC 2 cut(s) 245, 387
AclWI GGATC 2 cut(s) 334, 347
AcvI CACGTG 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 274
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 2 cut(s) 190, 217
AjiI CACGTC 1 cut(s) 211
AluBI AGCT 2 cut(s) 20, 326
AluI AGCT 2 cut(s) 20, 326
Alw21I GWGCWC 1 cut(s) 231
Alw44I GTGCAC 1 cut(s) 227
AlwI GGATC 2 cut(s) 334, 347
AoxI GGCC 1 cut(s) 277
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 3 cut(s) 261, 264, 296
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
AspS9I GGNCC 3 cut(s) 242, 277, 397
AsuHPI GGTGA 2 cut(s) 52, 61
AvaII GGWCC 2 cut(s) 242, 397
BaeGI GKGCMC 2 cut(s) 176, 231
BamHI GGATCC 1 cut(s) 339
BbrPI CACGTG 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 248, 276, 283
BfmI CTRYAG 2 cut(s) 15, 262
BfuAI ACCTGC 2 cut(s) 106, 385
BisI GCNGC 3 cut(s) 262, 265, 297
BlsI GCNGC 3 cut(s) 263, 266, 298
Bme18I GGWCC 2 cut(s) 242, 397
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 3 cut(s) 242, 277, 397
BmiI GGNNCC 2 cut(s) 341, 399
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 1 cut(s) 283
BmuI ACTGGG 1 cut(s) 170
Bpu10I CCTNAGC 1 cut(s) 120
BsaAI YACGTR 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 63
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse1I ACTGG 2 cut(s) 176, 409
BseDI CCNNGG 1 cut(s) 63
BseLI CCNNNNNNNGG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 2 cut(s) 176, 409
BseRI GAGGAG 3 cut(s) 23, 26, 41
BseSI GKGCMC 2 cut(s) 176, 231
BseXI GCAGC 3 cut(s) 248, 276, 283
BsgI GTGCAG 1 cut(s) 120
BshFI GGCC 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 231
BslFI GGGAC 3 cut(s) 123, 338, 383
BslI CCNNNNNNNGG 1 cut(s) 274
BsmFI GGGAC 3 cut(s) 123, 338, 383
BsnI GGCC 1 cut(s) 279
Bsp1286I GDGCHC 2 cut(s) 176, 231
Bsp143I GATC 1 cut(s) 339
BspACI CCGC 2 cut(s) 245, 387
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 1 cut(s) 357
BspLI GGNNCC 2 cut(s) 341, 399
BspMAI CTGCAG 1 cut(s) 266
BspMI ACCTGC 2 cut(s) 106, 385
BspPI GGATC 2 cut(s) 334, 347
BsrI ACTGG 2 cut(s) 176, 409
BssECI CCNNGG 1 cut(s) 63
BssMI GATC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 134
BstBAI YACGTR 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 126, 165, 371
BstDEI CTNAG 4 cut(s) 9, 120, 159, 365
BstEII GGTNACC 1 cut(s) 67
BstENI CCTNNNNNAGG 1 cut(s) 272
BstKTI GATC 1 cut(s) 342
BstMBI GATC 1 cut(s) 339
BstMWI GCNNNNNNNGC 5 cut(s) 11, 26, 134, 160, 323
BstPI GGTNACC 1 cut(s) 67
BstSFI CTRYAG 2 cut(s) 15, 262
BstSLI GKGCMC 2 cut(s) 176, 231
BstV1I GCAGC 3 cut(s) 248, 276, 283
BstX2I RGATCY 1 cut(s) 339
BstYI RGATCY 1 cut(s) 339
BsuRI GGCC 1 cut(s) 279
BtrI CACGTC 1 cut(s) 211
BtsI GCAGTG 2 cut(s) 24, 312
BtsIMutI CAGTG 3 cut(s) 24, 312, 362
BveI ACCTGC 2 cut(s) 106, 385
Cac8I GCNNGC 3 cut(s) 126, 165, 371
Cfr13I GGNCC 3 cut(s) 242, 277, 397
CspCI CAANNNNNGTGG 2 cut(s) 64, 99
CviAII CATG 1 cut(s) 258
CviJI RGCY 9 cut(s) 5, 14, 20, 154, 267, 279, 326, 369, 408
CviKI_1 RGCY 9 cut(s) 5, 14, 20, 154, 267, 279, 326, 369, 408
DdeI CTNAG 4 cut(s) 9, 120, 159, 365
DpnI GATC 1 cut(s) 341
DpnII GATC 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 63
Eco47I GGWCC 2 cut(s) 242, 397
Eco72I CACGTG 1 cut(s) 171
Eco91I GGTNACC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 2 cut(s) 277, 397
EcoO65I GGTNACC 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 63
FaeI CATG 1 cut(s) 261
FaiI YATR 2 cut(s) 202, 259
FaqI GGGAC 3 cut(s) 123, 338, 383
FatI CATG 1 cut(s) 257
FauI CCCGC 1 cut(s) 380
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 3 cut(s) 262, 265, 297
Fsp4HI GCNGC 3 cut(s) 262, 265, 297
GluI GCNGC 3 cut(s) 262, 265, 297
HaeIII GGCC 1 cut(s) 279
Hin1II CATG 1 cut(s) 261
HincII GTYRAC 2 cut(s) 85, 286
HindII GTYRAC 2 cut(s) 85, 286
HphI GGTGA 2 cut(s) 52, 61
Hpy166II GTNNAC 4 cut(s) 85, 181, 229, 286
Hpy8I GTNNAC 4 cut(s) 85, 181, 229, 286
HpyCH4IV ACGT 3 cut(s) 170, 210, 351
HpyCH4V TGCA 6 cut(s) 101, 137, 229, 264, 312, 317
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 26, 134, 160, 323
HpyF3I CTNAG 4 cut(s) 9, 120, 159, 365
HpySE526I ACGT 3 cut(s) 170, 210, 351
Hsp92II CATG 1 cut(s) 261
Kzo9I GATC 1 cut(s) 339
LpnPI CCDG 6 cut(s) 111, 147, 189, 380, 383, 390
Lsp1109I GCAGC 3 cut(s) 248, 276, 283
LweI GCATC 1 cut(s) 283
MaeII ACGT 3 cut(s) 170, 210, 351
MaeIII GTNAC 1 cut(s) 67
MalI GATC 1 cut(s) 341
MboI GATC 1 cut(s) 339
MboII GAAGA 2 cut(s) 46, 49
MflI RGATCY 1 cut(s) 339
MhlI GDGCHC 2 cut(s) 176, 231
MluCI AATT 1 cut(s) 191
MseI TTAA 2 cut(s) 347, 377
MwoI GCNNNNNNNGC 5 cut(s) 11, 26, 134, 160, 323
NdeII GATC 1 cut(s) 339
NlaIII CATG 1 cut(s) 261
NlaIV GGNNCC 2 cut(s) 341, 399
NmuCI GTSAC 1 cut(s) 67
PaqCI CACCTGC 1 cut(s) 106
PkrI GCNGC 3 cut(s) 263, 266, 298
PmaCI CACGTG 1 cut(s) 171
PmlI CACGTG 1 cut(s) 171
Ppu21I YACGTR 1 cut(s) 171
PpuMI RGGWCCY 1 cut(s) 397
Psp5II RGGWCCY 1 cut(s) 397
PspCI CACGTG 1 cut(s) 171
PspEI GGTNACC 1 cut(s) 67
PspN4I GGNNCC 2 cut(s) 341, 399
PspPI GGNCC 3 cut(s) 242, 277, 397
PspPPI RGGWCCY 1 cut(s) 397
PstI CTGCAG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 339
SaqAI TTAA 2 cut(s) 347, 377
SatI GCNGC 3 cut(s) 262, 265, 297
Sau3AI GATC 1 cut(s) 339
Sau96I GGNCC 3 cut(s) 242, 277, 397
SduI GDGCHC 2 cut(s) 176, 231
SfaNI GCATC 1 cut(s) 283
SfcI CTRYAG 2 cut(s) 15, 262
SinI GGWCC 2 cut(s) 242, 397
Sse9I AATT 1 cut(s) 191
SsiI CCGC 2 cut(s) 245, 387
StyI CCWWGG 1 cut(s) 63
TaiI ACGT 3 cut(s) 173, 213, 354
TasI AATT 1 cut(s) 191
Tru1I TTAA 2 cut(s) 347, 377
Tru9I TTAA 2 cut(s) 347, 377
TscAI CASTG 3 cut(s) 31, 319, 369
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 3 cut(s) 261, 264, 296
Tsp45I GTSAC 1 cut(s) 67
TspRI CASTG 3 cut(s) 31, 319, 369
VneI GTGCAC 1 cut(s) 227
VpaK11BI GGWCC 2 cut(s) 242, 397
XagI CCTNNNNNAGG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.