pycom06g09210

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010771
Physical Location & Seq
Reverse (-)
14426410 .. 14430954
4545 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2586 bp
ATGAATCCGCAAATCCGACATCCGTTGCTCTGGACCTTTGGGAGGGCCCACCGAAACAGAAAAACTGAAACACACCGAGAAAATCCCGGCCGCAAAGGAGACCCCGCCAGATTTGGATCTCTGATACAACGTTCGACTCTTTCGAACTCGATTCCGTCTTTTAATTCTTCTCAAAAATTTCTGTGGTTTCGATTTTCTAATCATTTTCTTGAAGATTCAAGCGGCAAGGAGGGCACAGAGATCCGTCCCCGGCTGGCCATGGTTAACAGAAGAGGTCAATGTAGGGAAAATGCCCTTTTCTTGTATAAGAGTTTTGGTTTGCTTTTCGGTGCTTTGAGCATTCCCCCTCTTTATGTTTATAAATGTGCATTTTCTGGAGGATTGCTCAATTACCAAACCGACGATGCGATATTTGGGGTGTTTTCAGTGATTTTTTGGACTCTTACTCTTATTTCTTTGCTAAAGTATGTCCTTGTCATGTTGAGCGCCAACGACAATGGTCAAGGTGGTGTTATTGCATTATACGCACTCCTTTGTAGAAATGCAAAATTCTGTTTGCTGCCTAATTATCAGGCATCTGATGAGGAGATCTCCACATACCGCTACCCCGGTTGTTCCAATACGAACAGGCCCACTTCTCCATTGAAAAGGTTTATCGAGAGACATAAAAGTTCAAAAACCTGTCTACTTCTTGTAGTATTATTTGGAGCTTCCATGGTGATTTGTGTTGGTATCCTCACTCCTGCAATATCAATTTTTGCATCTGTGGAGGGGCTACAATTTCAAGCAACGAATTTGCATAGTGGTGTGGTGGTTCTTATTGCCTGTATTTTATTGGTTGCCCTTTTTGTTTTGCAACACCGTGGCATCCACAAATTGGCCGTCATTTTCACTCCAATTATGATCCTTTGGCTACTGTTAATTGCTGCTGTTGGAATCTACAATATCATCGAGTGGAATCCAAGTGTATATCAGGCTCTTTCTCCTTATTACATTTACATATTCTTTAAGACGACAGGAAAAGATGGCTGGCTTTCTCTTGGAGGAATACTTTTATGTGTGACTGGAACTGAAGCCATCTTTGCGGATCTTGGCCACTTCAGAACAGCACCAGTAAGGGTTGCATTTTCTTTTGTGATTTACCCATGCCTAATACTTCAATACATGGGACAAGCTGCGTTTCTTTCGAGAAATCTATCTGCAGTATCTATGAGCTTTTTCGCTTCTGTTCCACCTCCCTTATTGTGGCCAGTACTTGTGGTTGCACCTTTGGCTGCCATTGTTGCCAGTCAACCTGTTATCTCTGCCACATTCTCAGTTGTCAAGCAGTGTCAGGCAATACGATGTTTCCCTCGCGTCAAGGTTGTACATACAAAGAGAAGGATATCTGGTCAGATATACATCCCTGAGTTGAACTGGTTTCTTATGATCATCAGTCTGGCTGTCACAATTGGATTTCAAGACACAATTCGTATAGCAAATGCTTATGGGATTGCATTTTTGGGTGTTACAATCATAACTACGTGGTTGGTATCGCTAGTCATCGACCTTGTTTGGCATCAGAGTTTCATGCTTTCCCTTTTATTTTGTGTACTTTTCGGTTCAGTTGAAGCCGTCTACCTTTCATCTTTGTGCGTGAGAATCCTCAAAGGTACATGGCTTCCCCTGGTTTTGTCTGCTGTCTTCTTGGTAGTTATGTATGTCTGGCAATATGGCACGCAGAAGAAGTATTTGTATGACTTGCATAACAAGGTTTCAATGAAGTGGATGCTCACGCTGGGTCCTAGTCTTGGCATTGTTAGGGTCCCTGGAATTGGCCTCATCTACACTGAATTGTCTACCGGAGTCCCAGCATCATTTACTCATTTCTTAACCAACCTACCGGCTTTTTACCAAGTGATTGTCTTCGTTTGTGTTAAAACTGTTCCTGTTCCTTATGTTCCCCAAAAAGAAAGGTATCTTGTTGGCCGTATTGGACCCAAATCTTTTGGGATGTACCGTTGTATGGTTCGGAATGGGTACAAAGATGTCTTTAAAAATGGGGACGACTTTGAAATTGATGTGGTGATGAGCATAGCAGAGTTCATCCAAATGGAAGCAGAAGGTTCTGGAACCCCTGAAGGCACTGTGGACCGCCGTATGGCAGTTGTGAAGACATCTGGGAAGTTTGGCACGGTGTTGGTTATGGCACAAACTTCTGGCCTTGGAGAAAGCAGCAGTTCAAGTTCTTCAGCGATTGTGAGCAGTGCCAAGTCTCCCACGCTGCAAAACTTGCAGGCCAACTACGAGCAAGAGGCACCAAAACTCAACTATTGGAGGCGGGCTCGGTTTGGATTACTGGATGCAAAATGCAAAGACTCCCACCTAAAGGAACAGCTCCAGGAACTTGTGGAAGCAAAACATGCCGGGGTAGCATATGTAATAGGTCAGGCATACTTAAAGGCGAAATGGAATTCATCGTTCTTAAAGAAGTGCGCTATCGATGTTGCCTACTCCTTCTTACGCAAGAATTGCCGCTCTCCCGCTGTTACTCTGAACATCCCTCATATTTGTTTGATCAAGGTGGGCATGAACTACATCGTGTAA

Protein Analysis

862

Amino Acids

95.98

Weight (kDa)

9.53

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 102 - 591 2.4e-144 K+ potassium transporter integral membrane domain
K_trans_C PF22776 603 - 859 6.7e-22 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010771)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23640
fragaria_vesca FvH4_5g03590 FvH4_5g03590 FvH4_5g03590 FvH4_5g03590
malus_domestica MD06G1095800.v1.1 MD14G1115800.v1.1 MD14G1115900.v1.1
prunus_persica Prupe.5G110400_v2.0.a1
pyrus_communis pycom06g09210
rosa_chinensis RchiOBHm_Chr7g0196121
rosa_laevigata RLG00000004062
rosa_multiflora Rmu_sc0003017.1_g000008
rosa_roxburghii Rroxscaffold_3G00259760
rosa_rugosa Rorug07G0030800
rosa_samantha Rh7AG156700 Rh7BG158400 Rh7CG162200 Rh7DG157500
rosa_wichuraiana Rw7G013380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AccB1I GGYRCC 1 cut(s) 2296
AccB7I CCANNNNNTGG 1 cut(s) 877
AccBSI CCGCTC 1 cut(s) 2517
AccI GTMKAC 3 cut(s) 685, 1617, 1838
AccII CGCG 1 cut(s) 1356
AclI AACGTT 1 cut(s) 130
AclWI GGATC 4 cut(s) 124, 235, 898, 1095
AcoI YGGCCR 6 cut(s) 88, 255, 879, 1093, 1247, 1966
AcsI RAATTY 4 cut(s) 176, 548, 793, 2452
AcuI CTGAAG 4 cut(s) 1084, 1092, 2139, 2214
AfaI GTAC 6 cut(s) 1254, 1368, 1593, 1654, 1997, 2021
AfiI CCNNNNNNNGG 8 cut(s) 42, 877, 1245, 1790, 1814, 2005, 2140, 2368
AjnI CCWGG 3 cut(s) 1665, 1807, 2379
AluBI AGCT 4 cut(s) 710, 1175, 1215, 2377
AluI AGCT 4 cut(s) 710, 1175, 1215, 2377
Alw26I GTCTC 3 cut(s) 93, 655, 2259
AlwI GGATC 4 cut(s) 124, 235, 898, 1095
ApaI GGGCCC 1 cut(s) 49
ApeKI GCWGC 6 cut(s) 559, 926, 1175, 1274, 2214, 2263
ApoI RAATTY 4 cut(s) 176, 548, 793, 2452
ArsI GACNNNNNNTTYG 2 cut(s) 867, 899
AspLEI GCGC 2 cut(s) 488, 2477
AspS9I GGNCC 8 cut(s) 33, 45, 46, 630, 1781, 1804, 1976, 2131
AsuC2I CCSGG 4 cut(s) 87, 250, 609, 2407
AsuHPI GGTGA 2 cut(s) 730, 2077
AsuII TTCGAA 1 cut(s) 143
AvaII GGWCC 5 cut(s) 33, 1781, 1804, 1976, 2131
BaeGI GKGCMC 2 cut(s) 49, 236
BalI TGGCCA 3 cut(s) 257, 1095, 1249
BanI GGYRCC 1 cut(s) 2296
BanII GRGCYC 2 cut(s) 49, 2326
BbsI GAAGAC 3 cut(s) 1675, 1897, 2159
BbvI GCAGC 6 cut(s) 546, 913, 1162, 1261, 2226, 2250
BccI CCATC 2 cut(s) 1019, 1085
BceAI ACGGC 4 cut(s) 866, 1598, 1953, 2121
BciT130I CCWGG 3 cut(s) 1667, 1809, 2381
BciVI GTATCC 1 cut(s) 743
BclI TGATCA 2 cut(s) 1428, 2556
BcnI CCSGG 4 cut(s) 87, 250, 609, 2407
BcoDI GTCTC 3 cut(s) 93, 655, 2259
BfaI CTAG 2 cut(s) 1538, 1785
BfmI CTRYAG 1 cut(s) 1200
BfoI RGCGCY 1 cut(s) 489
BfuI GTATCC 1 cut(s) 743
BglII AGATCT 1 cut(s) 588
BisI GCNGC 9 cut(s) 91, 223, 560, 927, 1176, 1275, 2215, 2264, 2515
BlsI GCNGC 9 cut(s) 92, 224, 561, 928, 1177, 1276, 2216, 2265, 2516
BmcAI AGTACT 1 cut(s) 1254
Bme1390I CCNGG 7 cut(s) 87, 250, 609, 1667, 1809, 2381, 2407
Bme18I GGWCC 5 cut(s) 33, 1781, 1804, 1976, 2131
BmgT120I GGNCC 8 cut(s) 33, 45, 46, 630, 1781, 1804, 1976, 2131
BmiI GGNNCC 7 cut(s) 47, 1782, 1805, 1806, 1978, 2113, 2298
BmrFI CCNGG 7 cut(s) 87, 250, 609, 1667, 1809, 2381, 2407
BmsI GCATC 8 cut(s) 394, 584, 770, 876, 1567, 1758, 1862, 2332
BoxI GACNNNNGTC 1 cut(s) 498
BpiI GAAGAC 3 cut(s) 1675, 1897, 2159
BplI GAGNNNNNCTC 6 cut(s) 369, 401, 575, 607, 2308, 2340
BpmI CTGGAG 2 cut(s) 396, 2363
Bpu14I TTCGAA 1 cut(s) 143
BpuMI CCSGG 4 cut(s) 87, 250, 609, 2407
Bsa29I ATCGAT 1 cut(s) 2481
BsaAI YACGTR 1 cut(s) 1524
BsaBI GATNNNNATC 2 cut(s) 115, 1400
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 9 cut(s) 248, 258, 607, 714, 862, 1665, 1807, 2203, 2406
BsaWI WCCGGW 1 cut(s) 1841
Bsc4I CCNNNNNNNGG 8 cut(s) 42, 877, 1245, 1790, 1814, 2005, 2140, 2368
Bse118I RCCGGY 1 cut(s) 1882
Bse1I ACTGG 6 cut(s) 1069, 1112, 1250, 1287, 1421, 2343
Bse8I GATNNNNATC 2 cut(s) 115, 1400
BseBI CCWGG 3 cut(s) 1667, 1809, 2381
BseCI ATCGAT 1 cut(s) 2481
BseDI CCNNGG 9 cut(s) 248, 258, 607, 714, 862, 1665, 1807, 2203, 2406
BseGI GGATG 8 cut(s) 19, 867, 1401, 1773, 1998, 2085, 2347, 2538
BseJI GATNNNNATC 2 cut(s) 115, 1400
BseLI CCNNNNNNNGG 8 cut(s) 42, 877, 1245, 1790, 1814, 2005, 2140, 2368
BseMII CTCAG 2 cut(s) 1329, 1398
BseNI ACTGG 6 cut(s) 1069, 1112, 1250, 1287, 1421, 2343
BseRI GAGGAG 1 cut(s) 599
BseSI GKGCMC 2 cut(s) 49, 236
BseX3I CGGCCG 1 cut(s) 88
BseXI GCAGC 6 cut(s) 546, 913, 1162, 1261, 2226, 2250
BseYI CCCAGC 2 cut(s) 1777, 1849
Bsh1236I CGCG 1 cut(s) 1356
Bsh1285I CGRYCG 1 cut(s) 91
BshNI GGYRCC 1 cut(s) 2296
BshVI ATCGAT 1 cut(s) 2481
BsiEI CGRYCG 1 cut(s) 91
BsiSI CCGG 6 cut(s) 87, 250, 609, 1842, 1883, 2406
BslFI GGGAC 5 cut(s) 231, 1182, 1790, 1832, 2057
BslI CCNNNNNNNGG 8 cut(s) 42, 877, 1245, 1790, 1814, 2005, 2140, 2368
BsmAI GTCTC 3 cut(s) 93, 655, 2259
BsmFI GGGAC 5 cut(s) 231, 1182, 1790, 1832, 2057
BsmI GAATGC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 143
Bsp120I GGGCCC 1 cut(s) 45
Bsp1286I GDGCHC 3 cut(s) 49, 236, 2326
Bsp1407I TGTACA 1 cut(s) 1366
Bsp143I GATC 7 cut(s) 116, 240, 588, 903, 1087, 1428, 2556
Bsp19I CCATGG 2 cut(s) 258, 714
BspCNI CTCAG 2 cut(s) 1328, 1399
BspDI ATCGAT 1 cut(s) 2481
BspFNI CGCG 1 cut(s) 1356
BspLI GGNNCC 7 cut(s) 47, 1782, 1805, 1806, 1978, 2113, 2298
BspMAI CTGCAG 1 cut(s) 1204
BspPI GGATC 4 cut(s) 124, 235, 898, 1095
BspT104I TTCGAA 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 2296
BspTNI GGTCTC 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 2517
BsrFI RCCGGY 1 cut(s) 1882
BsrGI TGTACA 1 cut(s) 1366
BsrI ACTGG 6 cut(s) 1069, 1112, 1250, 1287, 1421, 2343
BssAI RCCGGY 1 cut(s) 1882
BssECI CCNNGG 9 cut(s) 248, 258, 607, 714, 862, 1665, 1807, 2203, 2406
BssMI GATC 7 cut(s) 116, 240, 588, 903, 1087, 1428, 2556
BssT1I CCWWGG 3 cut(s) 258, 714, 2203
Bst2UI CCWGG 3 cut(s) 1667, 1809, 2381
Bst4CI ACNGT 6 cut(s) 863, 918, 1924, 2000, 2128, 2176
Bst6I CTCTTC 1 cut(s) 265
BstAPI GCANNNNNTGC 3 cut(s) 2272, 2402, 2511
BstAUI TGTACA 1 cut(s) 1366
BstBAI YACGTR 1 cut(s) 1524
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 5 cut(s) 255, 1031, 1718, 2277, 2322
BstDEI CTNAG 2 cut(s) 1315, 1407
BstDSI CCRYGG 3 cut(s) 258, 714, 862
BstENI CCTNNNNNAGG 1 cut(s) 40
BstF5I GGATG 8 cut(s) 19, 867, 1401, 1773, 1998, 2085, 2347, 2538
BstFNI CGCG 1 cut(s) 1356
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 2 cut(s) 488, 2477
BstKTI GATC 7 cut(s) 119, 243, 591, 906, 1090, 1431, 2559
BstMAI GTCTC 3 cut(s) 93, 655, 2259
BstMBI GATC 7 cut(s) 116, 240, 588, 903, 1087, 1428, 2556
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 9 cut(s) 231, 524, 1082, 1271, 1283, 2272, 2402, 2411, 2511
BstNI CCWGG 3 cut(s) 1667, 1809, 2381
BstNSI RCATGY 1 cut(s) 2405
BstPAI GACNNNNGTC 1 cut(s) 498
BstSCI CCNGG 7 cut(s) 85, 248, 607, 1665, 1807, 2379, 2405
BstSFI CTRYAG 1 cut(s) 1200
BstSLI GKGCMC 2 cut(s) 49, 236
BstUI CGCG 1 cut(s) 1356
BstV1I GCAGC 6 cut(s) 546, 913, 1162, 1261, 2226, 2250
BstV2I GAAGAC 3 cut(s) 1675, 1897, 2159
BstX2I RGATCY 4 cut(s) 116, 240, 588, 1087
BstYI RGATCY 4 cut(s) 116, 240, 588, 1087
BstZI CGGCCG 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 2481
BsuI GTATCC 1 cut(s) 743
BsuTUI ATCGAT 1 cut(s) 2481
BtgI CCRYGG 3 cut(s) 258, 714, 862
BtsCI GGATG 8 cut(s) 19, 867, 1401, 1773, 1998, 2085, 2347, 2538
BtsI GCAGTG 2 cut(s) 1334, 2251
BtsIMutI CAGTG 5 cut(s) 432, 1334, 1827, 2124, 2251
Cac8I GCNNGC 5 cut(s) 255, 1031, 1718, 2277, 2322
CfoI GCGC 2 cut(s) 488, 2477
Cfr10I RCCGGY 1 cut(s) 1882
Cfr13I GGNCC 8 cut(s) 33, 45, 46, 630, 1781, 1804, 1976, 2131
ClaI ATCGAT 1 cut(s) 2481
CseI GACGC 1 cut(s) 1345
Csp6I GTAC 6 cut(s) 1253, 1367, 1592, 1653, 1996, 2020
CviAII CATG 9 cut(s) 259, 478, 715, 1146, 1165, 1570, 1656, 2402, 2569
CviQI GTAC 6 cut(s) 1253, 1367, 1592, 1653, 1996, 2020
DdeI CTNAG 2 cut(s) 1315, 1407
DpnI GATC 7 cut(s) 118, 242, 590, 905, 1089, 1430, 2558
DpnII GATC 7 cut(s) 116, 240, 588, 903, 1087, 1428, 2556
DraI TTTAAA 1 cut(s) 2035
EaeI YGGCCR 6 cut(s) 88, 255, 879, 1093, 1247, 1966
EagI CGGCCG 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
EclXI CGGCCG 1 cut(s) 88
Eco130I CCWWGG 3 cut(s) 258, 714, 2203
Eco24I GRGCYC 2 cut(s) 49, 2326
Eco31I GGTCTC 1 cut(s) 93
Eco32I GATATC 1 cut(s) 1386
Eco47I GGWCC 5 cut(s) 33, 1781, 1804, 1976, 2131
Eco52I CGGCCG 1 cut(s) 88
Eco57I CTGAAG 4 cut(s) 1084, 1092, 2139, 2214
EcoNI CCTNNNNNAGG 1 cut(s) 40
EcoO109I RGGNCCY 3 cut(s) 45, 1781, 1804
EcoRI GAATTC 1 cut(s) 2452
EcoRII CCWGG 3 cut(s) 1665, 1807, 2379
EcoRV GATATC 1 cut(s) 1386
EcoT14I CCWWGG 3 cut(s) 258, 714, 2203
EcoT38I GRGCYC 2 cut(s) 49, 2326
ErhI CCWWGG 3 cut(s) 258, 714, 2203
FaeI CATG 9 cut(s) 262, 481, 718, 1149, 1168, 1573, 1659, 2405, 2572
FaqI GGGAC 5 cut(s) 231, 1182, 1790, 1832, 2057
FatI CATG 9 cut(s) 258, 477, 714, 1145, 1164, 1569, 1655, 2401, 2568
FauI CCCGC 3 cut(s) 112, 2313, 2530
FauNDI CATATG 1 cut(s) 2416
FbaI TGATCA 2 cut(s) 1428, 2556
FblI GTMKAC 3 cut(s) 685, 1617, 1838
Fnu4HI GCNGC 9 cut(s) 91, 223, 560, 927, 1176, 1275, 2215, 2264, 2515
FokI GGATG 8 cut(s) 6, 854, 1388, 1780, 2005, 2072, 2354, 2525
FriOI GRGCYC 2 cut(s) 49, 2326
Fsp4HI GCNGC 9 cut(s) 91, 223, 560, 927, 1176, 1275, 2215, 2264, 2515
FspBI CTAG 2 cut(s) 1538, 1785
GlaI GCGC 2 cut(s) 487, 2476
GluI GCNGC 9 cut(s) 91, 223, 560, 927, 1176, 1275, 2215, 2264, 2515
GsaI CCCAGC 2 cut(s) 1781, 1853
GsuI CTGGAG 2 cut(s) 396, 2363
HaeII RGCGCY 1 cut(s) 489
HapII CCGG 6 cut(s) 87, 250, 609, 1842, 1883, 2406
HgaI GACGC 1 cut(s) 1345
HhaI GCGC 2 cut(s) 488, 2477
Hin1II CATG 9 cut(s) 262, 481, 718, 1149, 1168, 1573, 1659, 2405, 2572
Hin6I GCGC 2 cut(s) 486, 2475
HinP1I GCGC 2 cut(s) 486, 2475
HincII GTYRAC 2 cut(s) 265, 1292
HindII GTYRAC 2 cut(s) 265, 1292
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 6 cut(s) 87, 250, 609, 1842, 1883, 2406
HphI GGTGA 2 cut(s) 730, 2077
Hpy166II GTNNAC 7 cut(s) 265, 686, 1292, 1592, 1618, 1839, 2131
Hpy188I TCNGA 8 cut(s) 17, 123, 580, 1103, 1395, 1563, 2013, 2535
Hpy188III TCNNGA 7 cut(s) 31, 209, 375, 658, 1188, 1460, 2109
Hpy8I GTNNAC 7 cut(s) 265, 686, 1292, 1592, 1618, 1839, 2131
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 4 cut(s) 1374, 2096, 2114, 2506
HpyCH4III ACNGT 6 cut(s) 863, 918, 1924, 2000, 2128, 2176
HpyCH4IV ACGT 2 cut(s) 130, 1523
HpyF10VI GCNNNNNNNGC 9 cut(s) 231, 524, 1082, 1271, 1283, 2272, 2402, 2411, 2511
HpyF3I CTNAG 2 cut(s) 1315, 1407
HpySE526I ACGT 2 cut(s) 130, 1523
Hsp92II CATG 9 cut(s) 262, 481, 718, 1149, 1168, 1573, 1659, 2405, 2572
HspAI GCGC 2 cut(s) 486, 2475
KflI GGGWCCC 1 cut(s) 1804
Ksp22I TGATCA 2 cut(s) 1428, 2556
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 7 cut(s) 116, 240, 588, 903, 1087, 1428, 2556
LmnI GCTCC 2 cut(s) 707, 2382
Lsp1109I GCAGC 6 cut(s) 546, 913, 1162, 1261, 2226, 2250
LweI GCATC 8 cut(s) 394, 584, 770, 876, 1567, 1758, 1862, 2332
MaeI CTAG 2 cut(s) 1538, 1785
MaeII ACGT 2 cut(s) 130, 1523
MaeIII GTNAC 4 cut(s) 1060, 1444, 1507, 2527
MalI GATC 7 cut(s) 118, 242, 590, 905, 1089, 1430, 2558
MbiI CCGCTC 1 cut(s) 2517
MboI GATC 7 cut(s) 116, 240, 588, 903, 1087, 1428, 2556
MboII GAAGA 8 cut(s) 159, 224, 282, 1675, 1735, 1897, 2164, 2220
MfeI CAATTG 1 cut(s) 1449
MflI RGATCY 4 cut(s) 116, 240, 588, 1087
MhlI GDGCHC 3 cut(s) 49, 236, 2326
MlsI TGGCCA 3 cut(s) 257, 1095, 1249
MluNI TGGCCA 3 cut(s) 257, 1095, 1249
MlyI GAGTC 4 cut(s) 130, 433, 1854, 2351
MmeI TCCRAC 2 cut(s) 40, 913
Mox20I TGGCCA 3 cut(s) 257, 1095, 1249
MscI TGGCCA 3 cut(s) 257, 1095, 1249
MseI TTAA 9 cut(s) 162, 264, 920, 1008, 1871, 1917, 2034, 2438, 2465
MslI CAYNNNNRTG 3 cut(s) 804, 1630, 2090
Msp20I TGGCCA 3 cut(s) 257, 1095, 1249
MspA1I CMGCKG 1 cut(s) 2525
MspI CCGG 6 cut(s) 87, 250, 609, 1842, 1883, 2406
MspR9I CCNGG 7 cut(s) 87, 250, 609, 1667, 1809, 2381, 2407
MunI CAATTG 1 cut(s) 1449
Mva1269I GAATGC 1 cut(s) 339
MvaI CCWGG 3 cut(s) 1667, 1809, 2381
MvnI CGCG 1 cut(s) 1356
MwoI GCNNNNNNNGC 9 cut(s) 231, 524, 1082, 1271, 1283, 2272, 2402, 2411, 2511
NciI CCSGG 4 cut(s) 87, 250, 609, 2407
NcoI CCATGG 2 cut(s) 258, 714
NdeI CATATG 1 cut(s) 2416
NdeII GATC 7 cut(s) 116, 240, 588, 903, 1087, 1428, 2556
NlaIII CATG 9 cut(s) 262, 481, 718, 1149, 1168, 1573, 1659, 2405, 2572
NlaIV GGNNCC 7 cut(s) 47, 1782, 1805, 1806, 1978, 2113, 2298
NmuCI GTSAC 2 cut(s) 1060, 1444
NspI RCATGY 1 cut(s) 2405
NspV TTCGAA 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 1 cut(s) 339
PfeI GAWTC 6 cut(s) 4, 151, 215, 936, 958, 1641
PflMI CCANNNNNTGG 1 cut(s) 877
PfoI TCCNGGA 1 cut(s) 2379
PkrI GCNGC 9 cut(s) 92, 224, 561, 928, 1177, 1276, 2216, 2265, 2516
PleI GAGTC 4 cut(s) 130, 433, 1853, 2351
PpsI GAGTC 4 cut(s) 130, 433, 1853, 2351
Ppu21I YACGTR 1 cut(s) 1524
PpuMI RGGWCCY 2 cut(s) 1781, 1804
PshAI GACNNNNGTC 1 cut(s) 498
PsiI TTATAA 1 cut(s) 360
Psp1406I AACGTT 1 cut(s) 130
Psp5II RGGWCCY 2 cut(s) 1781, 1804
Psp6I CCWGG 3 cut(s) 1665, 1807, 2379
PspFI CCCAGC 2 cut(s) 1777, 1849
PspGI CCWGG 3 cut(s) 1665, 1807, 2379
PspN4I GGNNCC 7 cut(s) 47, 1782, 1805, 1806, 1978, 2113, 2298
PspOMI GGGCCC 1 cut(s) 45
PspPI GGNCC 8 cut(s) 33, 45, 46, 630, 1781, 1804, 1976, 2131
PspPPI RGGWCCY 2 cut(s) 1781, 1804
PstI CTGCAG 1 cut(s) 1204
PsuI RGATCY 4 cut(s) 116, 240, 588, 1087
RsaI GTAC 6 cut(s) 1254, 1368, 1593, 1654, 1997, 2021
RsaNI GTAC 6 cut(s) 1253, 1367, 1592, 1653, 1996, 2020
RseI CAYNNNNRTG 3 cut(s) 804, 1630, 2090
SaqAI TTAA 9 cut(s) 162, 264, 920, 1008, 1871, 1917, 2034, 2438, 2465
SatI GCNGC 9 cut(s) 91, 223, 560, 927, 1176, 1275, 2215, 2264, 2515
Sau3AI GATC 7 cut(s) 116, 240, 588, 903, 1087, 1428, 2556
Sau96I GGNCC 8 cut(s) 33, 45, 46, 630, 1781, 1804, 1976, 2131
ScaI AGTACT 1 cut(s) 1254
SchI GAGTC 4 cut(s) 130, 433, 1854, 2351
ScrFI CCNGG 7 cut(s) 87, 250, 609, 1667, 1809, 2381, 2407
SduI GDGCHC 3 cut(s) 49, 236, 2326
SfaNI GCATC 8 cut(s) 394, 584, 770, 876, 1567, 1758, 1862, 2332
SfcI CTRYAG 1 cut(s) 1200
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 5 cut(s) 33, 1781, 1804, 1976, 2131
SmiMI CAYNNNNRTG 3 cut(s) 804, 1630, 2090
SspMI CTAG 2 cut(s) 1538, 1785
StyD4I CCNGG 7 cut(s) 85, 248, 607, 1665, 1807, 2379, 2405
StyI CCWWGG 3 cut(s) 258, 714, 2203
TaaI ACNGT 6 cut(s) 863, 918, 1924, 2000, 2128, 2176
TaiI ACGT 2 cut(s) 133, 1526
TaqI TCGA 9 cut(s) 134, 143, 149, 190, 657, 951, 1187, 1545, 2481
TatI WGTACW 3 cut(s) 1252, 1366, 1591
TauI GCSGC 3 cut(s) 93, 225, 2517
TfiI GAWTC 6 cut(s) 4, 151, 215, 936, 958, 1641
Tru1I TTAA 9 cut(s) 162, 264, 920, 1008, 1871, 1917, 2034, 2438, 2465
Tru9I TTAA 9 cut(s) 162, 264, 920, 1008, 1871, 1917, 2034, 2438, 2465
TscAI CASTG 5 cut(s) 432, 1334, 1834, 2131, 2251
TseFI GTSAC 2 cut(s) 1060, 1444
TseI GCWGC 6 cut(s) 559, 926, 1175, 1274, 2214, 2263
Tsp45I GTSAC 2 cut(s) 1060, 1444
TspDTI ATGAA 7 cut(s) 17, 1558, 1614, 1775, 2074, 2445, 2585
TspGWI ACGGA 3 cut(s) 12, 144, 233
TspRI CASTG 5 cut(s) 432, 1334, 1834, 2131, 2251
Van91I CCANNNNNTGG 1 cut(s) 877
VpaK11BI GGWCC 5 cut(s) 33, 1781, 1804, 1976, 2131
XagI CCTNNNNNAGG 1 cut(s) 40
XapI RAATTY 4 cut(s) 176, 548, 793, 2452
XceI RCATGY 1 cut(s) 2405
XmiI GTMKAC 3 cut(s) 685, 1617, 1838
XspI CTAG 2 cut(s) 1538, 1785
ZrmI AGTACT 1 cut(s) 1254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.