pycom06g13320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0021246
Physical Location & Seq
Forward (+)
18346711 .. 18347160
450 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13320.1

Sequence Viewer

Length: 450 bp
ATGTATACACACAAGAGCACTGCAACTGCATACTGTCACTGCATCATGATTCTGAACTCTTCAAAGCAAATCACCCCATCTCCATTCAAATCAAACCGTTGGATCATCACCTTGCACTCATCAACGGACTTGGACTCACCCAACTTGCTCAGCATTCTCTTCAAGCTGCTGGGTGTAATAAACCCACACCCCTCCACATCATACACCTCAAAAGCCTCCCTTAAACCCTTCACCTTCTCCTCCTCTTCCCCTCCTTCCATTAACCCCACAAGATCCTCCAAACCCAGCAACCCGTCTCCGTCCGAGTCCAGCATTTCCACTGCCGCCTCCGCCTCATTCTGCAGCAGCTCTCCACCCATTAACCCTAGCCGGCGCCTGAGCTCCGCAGGCGAAACCTTTCCGTCACCATCTTCGTCAAAGTATCTGAAAACACGTTCGTATCCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.03

Weight (kDa)

9.91

Isoelectric Point (pI)

84.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021246)

Species Orthologous Gene IDs
prunus_persica Prupe.1G332900_v2.0.a1
pyrus_communis pycom06g13320 pycom111g01150 pycom17g01130
rosa_samantha Rh3AG309900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 372
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 324, 330, 384
AclWI GGATC 2 cut(s) 110, 267
AcyI GRCGYC 1 cut(s) 373
AflIII ACRYGT 1 cut(s) 431
AgsI TTSAA 3 cut(s) 63, 88, 163
AluBI AGCT 3 cut(s) 166, 348, 381
AluI AGCT 3 cut(s) 166, 348, 381
Alw21I GWGCWC 2 cut(s) 20, 383
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 2 cut(s) 110, 267
ApeKI GCWGC 3 cut(s) 166, 342, 345
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 375
AsuHPI GGTGA 5 cut(s) 64, 100, 129, 223, 396
BaeI ACNNNNGTAYC 1 cut(s) 422
BanI GGYRCC 1 cut(s) 372
BanII GRGCYC 1 cut(s) 383
Bbv12I GWGCWC 2 cut(s) 20, 383
BbvI GCAGC 3 cut(s) 153, 354, 357
BccI CCATC 2 cut(s) 85, 415
BciVI GTATCC 1 cut(s) 450
BcoDI GTCTC 1 cut(s) 300
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 1 cut(s) 340
BfoI RGCGCY 1 cut(s) 376
BfuI GTATCC 1 cut(s) 450
BisI GCNGC 4 cut(s) 167, 324, 343, 346
BlpI GCTNAGC 1 cut(s) 149
BlsI GCNGC 4 cut(s) 168, 325, 344, 347
BmiI GGNNCC 1 cut(s) 374
BmsI GCATC 1 cut(s) 51
Bpu10I CCTNAGC 1 cut(s) 377
Bpu1102I GCTNAGC 1 cut(s) 149
BsaHI GRCGYC 1 cut(s) 373
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bse118I RCCGGY 1 cut(s) 369
BseMII CTCAG 2 cut(s) 163, 368
BseRI GAGGAG 2 cut(s) 229, 232
BseXI GCAGC 3 cut(s) 153, 354, 357
BseYI CCCAGC 2 cut(s) 169, 284
BshNI GGYRCC 1 cut(s) 372
BsiHKAI GWGCWC 2 cut(s) 20, 383
BsiSI CCGG 1 cut(s) 370
BsmAI GTCTC 1 cut(s) 300
BsmBI CGTCTC 1 cut(s) 300
BsmI GAATGC 1 cut(s) 153
Bsp1286I GDGCHC 2 cut(s) 20, 383
Bsp143I GATC 2 cut(s) 102, 272
Bsp1720I GCTNAGC 1 cut(s) 149
BspACI CCGC 3 cut(s) 324, 330, 384
BspCNI CTCAG 2 cut(s) 162, 369
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 1 cut(s) 374
BspMAI CTGCAG 1 cut(s) 344
BspPI GGATC 2 cut(s) 110, 267
BspT107I GGYRCC 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 369
BssAI RCCGGY 1 cut(s) 369
BssMI GATC 2 cut(s) 102, 272
BssNAI GTATAC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 373
Bst1107I GTATAC 1 cut(s) 6
Bst4CI ACNGT 2 cut(s) 35, 98
Bst6I CTCTTC 3 cut(s) 64, 164, 250
BstACI GRCGYC 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 371, 388
BstDEI CTNAG 2 cut(s) 149, 377
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 2 cut(s) 105, 275
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 2 cut(s) 102, 272
BstMWI GCNNNNNNNGC 2 cut(s) 329, 387
BstSFI CTRYAG 1 cut(s) 340
BstV1I GCAGC 3 cut(s) 153, 354, 357
BstX2I RGATCY 1 cut(s) 272
BstYI RGATCY 1 cut(s) 272
BstZ17I GTATAC 1 cut(s) 6
BsuI GTATCC 1 cut(s) 450
BtsI GCAGTG 3 cut(s) 18, 37, 318
BtsIMutI CAGTG 3 cut(s) 18, 37, 318
Cac8I GCNNGC 2 cut(s) 371, 388
CciI TCATGA 1 cut(s) 45
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 369
CviAII CATG 1 cut(s) 46
CviJI RGCY 5 cut(s) 166, 215, 348, 369, 381
CviKI_1 RGCY 5 cut(s) 166, 215, 348, 369, 381
DdeI CTNAG 2 cut(s) 149, 377
DinI GGCGCC 1 cut(s) 374
DpnI GATC 2 cut(s) 104, 274
DpnII GATC 2 cut(s) 102, 272
Eam1104I CTCTTC 3 cut(s) 64, 164, 250
EarI CTCTTC 3 cut(s) 64, 164, 250
EciI GGCGGA 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 381
Eco24I GRGCYC 1 cut(s) 383
Eco53kI GAGCTC 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 383
EgeI GGCGCC 1 cut(s) 374
EheI GGCGCC 1 cut(s) 374
Esp3I CGTCTC 1 cut(s) 300
FaeI CATG 1 cut(s) 49
FaiI YATR 4 cut(s) 6, 31, 47, 202
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 1 cut(s) 45
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 167, 324, 343, 346
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 4 cut(s) 167, 324, 343, 346
FspBI CTAG 1 cut(s) 366
GlaI GCGC 1 cut(s) 374
GluI GCNGC 4 cut(s) 167, 324, 343, 346
GsaI CCCAGC 2 cut(s) 173, 288
HaeII RGCGCY 1 cut(s) 376
HapII CCGG 1 cut(s) 370
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 373
Hin1II CATG 1 cut(s) 49
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HinfI GANTC 3 cut(s) 49, 134, 305
HpaII CCGG 1 cut(s) 370
HphI GGTGA 5 cut(s) 64, 100, 129, 223, 396
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 54, 304, 426
Hpy188III TCNNGA 1 cut(s) 46
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 3 cut(s) 238, 244, 264
HpyCH4III ACNGT 2 cut(s) 35, 98
HpyCH4IV ACGT 1 cut(s) 433
HpyCH4V TGCA 5 cut(s) 23, 29, 42, 115, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 387
HpyF3I CTNAG 2 cut(s) 149, 377
HpySE526I ACGT 1 cut(s) 433
Hsp92I GRCGYC 1 cut(s) 373
Hsp92II CATG 1 cut(s) 49
HspAI GCGC 1 cut(s) 373
KasI GGCGCC 1 cut(s) 372
KroI GCCGGC 1 cut(s) 369
KroNI GCCGGC 1 cut(s) 371
Kzo9I GATC 2 cut(s) 102, 272
LmnI GCTCC 1 cut(s) 386
LpnPI CCDG 6 cut(s) 155, 298, 322, 372, 383, 389
Lsp1109I GCAGC 3 cut(s) 153, 354, 357
LweI GCATC 1 cut(s) 51
MaeI CTAG 1 cut(s) 366
MaeII ACGT 1 cut(s) 433
MaeIII GTNAC 2 cut(s) 35, 402
MalI GATC 2 cut(s) 104, 274
MboI GATC 2 cut(s) 102, 272
MboII GAAGA 4 cut(s) 51, 151, 237, 402
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 2 cut(s) 20, 383
Mly113I GGCGCC 1 cut(s) 373
MlyI GAGTC 2 cut(s) 128, 314
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 9 cut(s) 202, 217, 226, 250, 253, 261, 286, 337, 343
MroNI GCCGGC 1 cut(s) 369
MroXI GAANNNNTTC 1 cut(s) 396
MseI TTAA 3 cut(s) 222, 261, 360
MspI CCGG 1 cut(s) 370
Mva1269I GAATGC 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 329, 387
NaeI GCCGGC 1 cut(s) 371
NarI GGCGCC 1 cut(s) 373
NdeII GATC 2 cut(s) 102, 272
NgoMIV GCCGGC 1 cut(s) 369
NlaIII CATG 1 cut(s) 49
NlaIV GGNNCC 1 cut(s) 374
NmuCI GTSAC 2 cut(s) 35, 402
PagI TCATGA 1 cut(s) 45
PctI GAATGC 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 371
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 1 cut(s) 49
PkrI GCNGC 4 cut(s) 168, 325, 344, 347
PleI GAGTC 2 cut(s) 128, 313
PluTI GGCGCC 1 cut(s) 376
PpsI GAGTC 2 cut(s) 128, 313
Psp124BI GAGCTC 1 cut(s) 383
PspFI CCCAGC 2 cut(s) 169, 284
PspN4I GGNNCC 1 cut(s) 374
PstI CTGCAG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 272
SacI GAGCTC 1 cut(s) 383
SaqAI TTAA 3 cut(s) 222, 261, 360
SatI GCNGC 4 cut(s) 167, 324, 343, 346
Sau3AI GATC 2 cut(s) 102, 272
SchI GAGTC 2 cut(s) 128, 314
SduI GDGCHC 2 cut(s) 20, 383
SetI ASST 8 cut(s) 113, 168, 209, 236, 350, 383, 398, 436
SfaNI GCATC 1 cut(s) 51
SfcI CTRYAG 1 cut(s) 340
SfoI GGCGCC 1 cut(s) 374
SsiI CCGC 3 cut(s) 324, 330, 384
SspDI GGCGCC 1 cut(s) 372
SspMI CTAG 1 cut(s) 366
SstI GAGCTC 1 cut(s) 383
TaaI ACNGT 2 cut(s) 35, 98
TaiI ACGT 1 cut(s) 436
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 3 cut(s) 222, 261, 360
Tru9I TTAA 3 cut(s) 222, 261, 360
TscAI CASTG 3 cut(s) 25, 44, 325
TseFI GTSAC 2 cut(s) 35, 402
TseI GCWGC 3 cut(s) 166, 342, 345
Tsp45I GTSAC 2 cut(s) 35, 402
TspGWI ACGGA 3 cut(s) 140, 288, 390
TspRI CASTG 3 cut(s) 25, 44, 325
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.