Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g17280 |
| malus_domestica | MD06G1226500.v1.1 MD14G1235900.v1.1 |
| prunus_persica | Prupe.5G232700_v2.0.a1 |
| pyrus_communis | pycom06g20280 |
| rosa_chinensis | RchiOBHm_Chr7g0178491 |
| rosa_laevigata | RLG00000005442 |
| rosa_multiflora | Rmu_sc0010235.1_g000002 |
| rosa_rugosa | Rorug06G0417600 |
| rosa_wichuraiana | Rw7G001270 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1085 |
| Acc16I | TGCGCA | 2 cut(s) | 847, 904 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 60, 665 |
| AccBSI | CCGCTC | 1 cut(s) | 702 |
| AccI | GTMKAC | 2 cut(s) | 718, 1193 |
| AccII | CGCG | 4 cut(s) | 186, 188, 231, 916 |
| AccIII | TCCGGA | 1 cut(s) | 478 |
| AclWI | GGATC | 3 cut(s) | 753, 838, 905 |
| AcoI | YGGCCR | 3 cut(s) | 169, 244, 277 |
| AcsI | RAATTY | 3 cut(s) | 473, 766, 1108 |
| AcuI | CTGAAG | 1 cut(s) | 619 |
| AfaI | GTAC | 4 cut(s) | 107, 180, 842, 1274 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 60, 286, 665, 920, 1076 |
| AflII | CTTAAG | 1 cut(s) | 644 |
| AgeI | ACCGGT | 2 cut(s) | 110, 428 |
| AgsI | TTSAA | 4 cut(s) | 401, 459, 676, 739 |
| AjnI | CCWGG | 3 cut(s) | 21, 208, 727 |
| AleI | CACNNNNGTG | 1 cut(s) | 536 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 995, 1027 |
| AluBI | AGCT | 4 cut(s) | 334, 649, 663, 757 |
| AluI | AGCT | 4 cut(s) | 334, 649, 663, 757 |
| Alw21I | GWGCWC | 5 cut(s) | 84, 195, 336, 351, 968 |
| AlwI | GGATC | 3 cut(s) | 753, 838, 905 |
| Ama87I | CYCGRG | 2 cut(s) | 203, 825 |
| Aor13HI | TCCGGA | 1 cut(s) | 478 |
| AoxI | GGCC | 7 cut(s) | 159, 169, 236, 244, 277, 288, 1176 |
| ApeKI | GCWGC | 2 cut(s) | 835, 905 |
| ApoI | RAATTY | 3 cut(s) | 473, 766, 1108 |
| AsiGI | ACCGGT | 2 cut(s) | 110, 428 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 430, 474 |
| AspLEI | GCGC | 6 cut(s) | 188, 233, 329, 848, 905, 918 |
| AspS9I | GGNCC | 5 cut(s) | 199, 236, 288, 481, 601 |
| AsuC2I | CCSGG | 2 cut(s) | 100, 286 |
| AsuHPI | GGTGA | 4 cut(s) | 151, 487, 560, 643 |
| AsuII | TTCGAA | 1 cut(s) | 1135 |
| AsuNHI | GCTAGC | 1 cut(s) | 757 |
| AvaI | CYCGRG | 2 cut(s) | 203, 825 |
| AvaII | GGWCC | 3 cut(s) | 199, 481, 601 |
| BalI | TGGCCA | 2 cut(s) | 246, 279 |
| BanII | GRGCYC | 1 cut(s) | 336 |
| BbsI | GAAGAC | 1 cut(s) | 1083 |
| Bbv12I | GWGCWC | 5 cut(s) | 84, 195, 336, 351, 968 |
| BbvI | GCAGC | 2 cut(s) | 847, 917 |
| BccI | CCATC | 4 cut(s) | 520, 1210, 1292, 1321 |
| BceAI | ACGGC | 4 cut(s) | 100, 156, 746, 1173 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1238, 1272 |
| BciT130I | CCWGG | 3 cut(s) | 23, 210, 729 |
| BcnI | CCSGG | 2 cut(s) | 100, 286 |
| BfaI | CTAG | 1 cut(s) | 758 |
| BfoI | RGCGCY | 1 cut(s) | 330 |
| BfrI | CTTAAG | 1 cut(s) | 644 |
| BisI | GCNGC | 5 cut(s) | 166, 169, 836, 906, 1073 |
| BlpI | GCTNAGC | 1 cut(s) | 330 |
| BlsI | GCNGC | 5 cut(s) | 167, 170, 837, 907, 1074 |
| BmcAI | AGTACT | 1 cut(s) | 842 |
| Bme1390I | CCNGG | 5 cut(s) | 23, 100, 210, 286, 729 |
| Bme18I | GGWCC | 3 cut(s) | 199, 481, 601 |
| BmeT110I | CYCGRG | 2 cut(s) | 203, 825 |
| BmgT120I | GGNCC | 5 cut(s) | 199, 236, 288, 481, 601 |
| BmiI | GGNNCC | 5 cut(s) | 41, 52, 289, 427, 543 |
| BmrFI | CCNGG | 5 cut(s) | 23, 100, 210, 286, 729 |
| BmsI | GCATC | 5 cut(s) | 234, 480, 874, 947, 1270 |
| BmtI | GCTAGC | 1 cut(s) | 761 |
| BpiI | GAAGAC | 1 cut(s) | 1083 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 1329 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 330 |
| Bpu14I | TTCGAA | 1 cut(s) | 1135 |
| BpuMI | CCSGG | 2 cut(s) | 100, 286 |
| BsaAI | YACGTR | 1 cut(s) | 178 |
| BsaBI | GATNNNNATC | 1 cut(s) | 675 |
| BsaJI | CCNNGG | 5 cut(s) | 22, 285, 727, 919, 1284 |
| BsaWI | WCCGGW | 5 cut(s) | 110, 259, 428, 478, 1196 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 56, 86, 995, 1025 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 60, 286, 665, 920, 1076 |
| Bse118I | RCCGGY | 2 cut(s) | 110, 428 |
| Bse1I | ACTGG | 3 cut(s) | 280, 579, 1193 |
| Bse3DI | GCAATG | 2 cut(s) | 38, 1167 |
| Bse8I | GATNNNNATC | 1 cut(s) | 675 |
| BseAI | TCCGGA | 1 cut(s) | 478 |
| BseBI | CCWGG | 3 cut(s) | 23, 210, 729 |
| BseDI | CCNNGG | 5 cut(s) | 22, 285, 727, 919, 1284 |
| BseGI | GGATG | 5 cut(s) | 18, 225, 889, 1216, 1332 |
| BseJI | GATNNNNATC | 1 cut(s) | 675 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 60, 286, 665, 920, 1076 |
| BseMI | GCAATG | 2 cut(s) | 38, 1167 |
| BseMII | CTCAG | 2 cut(s) | 321, 363 |
| BseNI | ACTGG | 3 cut(s) | 280, 579, 1193 |
| BseRI | GAGGAG | 2 cut(s) | 440, 1003 |
| BseX3I | CGGCCG | 1 cut(s) | 169 |
| BseXI | GCAGC | 2 cut(s) | 847, 917 |
| BsgI | GTGCAG | 1 cut(s) | 1019 |
| Bsh1236I | CGCG | 4 cut(s) | 186, 188, 231, 916 |
| Bsh1285I | CGRYCG | 2 cut(s) | 172, 260 |
| BshFI | GGCC | 7 cut(s) | 161, 171, 238, 246, 279, 290, 1178 |
| BshTI | ACCGGT | 2 cut(s) | 110, 428 |
| BsiEI | CGRYCG | 2 cut(s) | 172, 260 |
| BsiHKAI | GWGCWC | 5 cut(s) | 84, 195, 336, 351, 968 |
| BsiHKCI | CYCGRG | 2 cut(s) | 203, 825 |
| BsiSI | CCGG | 8 cut(s) | 99, 111, 260, 285, 291, 429, 479, 1197 |
| BslFI | GGGAC | 1 cut(s) | 842 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 60, 286, 665, 920, 1076 |
| BsmFI | GGGAC | 1 cut(s) | 842 |
| BsnI | GGCC | 7 cut(s) | 161, 171, 238, 246, 279, 290, 1178 |
| BsoBI | CYCGRG | 2 cut(s) | 203, 825 |
| Bsp119I | TTCGAA | 1 cut(s) | 1135 |
| Bsp1286I | GDGCHC | 5 cut(s) | 84, 195, 336, 351, 968 |
| Bsp13I | TCCGGA | 1 cut(s) | 478 |
| Bsp143I | GATC | 4 cut(s) | 745, 830, 910, 1249 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 330 |
| Bsp19I | CCATGG | 1 cut(s) | 919 |
| BspANI | GGCC | 7 cut(s) | 161, 171, 238, 246, 279, 290, 1178 |
| BspCNI | CTCAG | 2 cut(s) | 322, 364 |
| BspEI | TCCGGA | 1 cut(s) | 478 |
| BspFNI | CGCG | 4 cut(s) | 186, 188, 231, 916 |
| BspLI | GGNNCC | 5 cut(s) | 41, 52, 289, 427, 543 |
| BspOI | GCTAGC | 1 cut(s) | 761 |
| BspPI | GGATC | 3 cut(s) | 753, 838, 905 |
| BspT104I | TTCGAA | 1 cut(s) | 1135 |
| BspTI | CTTAAG | 1 cut(s) | 644 |
| BsrBI | CCGCTC | 1 cut(s) | 702 |
| BsrDI | GCAATG | 2 cut(s) | 38, 1167 |
| BsrFI | RCCGGY | 2 cut(s) | 110, 428 |
| BsrI | ACTGG | 3 cut(s) | 280, 579, 1193 |
| BssAI | RCCGGY | 2 cut(s) | 110, 428 |
| BssECI | CCNNGG | 5 cut(s) | 22, 285, 727, 919, 1284 |
| BssMI | GATC | 4 cut(s) | 745, 830, 910, 1249 |
| BssNAI | GTATAC | 1 cut(s) | 1194 |
| BssT1I | CCWWGG | 1 cut(s) | 919 |
| Bst1107I | GTATAC | 1 cut(s) | 1194 |
| Bst2UI | CCWGG | 3 cut(s) | 23, 210, 729 |
| Bst4CI | ACNGT | 4 cut(s) | 380, 535, 1272, 1288 |
| Bst6I | CTCTTC | 2 cut(s) | 1132, 1308 |
| BstAFI | CTTAAG | 1 cut(s) | 644 |
| BstBAI | YACGTR | 1 cut(s) | 178 |
| BstBI | TTCGAA | 1 cut(s) | 1135 |
| BstC8I | GCNNGC | 4 cut(s) | 163, 389, 759, 1148 |
| BstDEI | CTNAG | 2 cut(s) | 330, 372 |
| BstDSI | CCRYGG | 2 cut(s) | 919, 1284 |
| BstF5I | GGATG | 5 cut(s) | 18, 225, 889, 1216, 1332 |
| BstFNI | CGCG | 4 cut(s) | 186, 188, 231, 916 |
| BstH2I | RGCGCY | 1 cut(s) | 330 |
| BstHHI | GCGC | 6 cut(s) | 188, 233, 329, 848, 905, 918 |
| BstKTI | GATC | 4 cut(s) | 748, 833, 913, 1252 |
| BstMBI | GATC | 4 cut(s) | 745, 830, 910, 1249 |
| BstMCI | CGRYCG | 2 cut(s) | 172, 260 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 922, 963 |
| BstNI | CCWGG | 3 cut(s) | 23, 210, 729 |
| BstSCI | CCNGG | 5 cut(s) | 21, 98, 208, 284, 727 |
| BstSNI | TACGTA | 1 cut(s) | 178 |
| BstUI | CGCG | 4 cut(s) | 186, 188, 231, 916 |
| BstV1I | GCAGC | 2 cut(s) | 847, 917 |
| BstV2I | GAAGAC | 1 cut(s) | 1083 |
| BstX2I | RGATCY | 2 cut(s) | 745, 910 |
| BstYI | RGATCY | 2 cut(s) | 745, 910 |
| BstZ17I | GTATAC | 1 cut(s) | 1194 |
| BstZI | CGGCCG | 1 cut(s) | 169 |
| BsuRI | GGCC | 7 cut(s) | 161, 171, 238, 246, 279, 290, 1178 |
| BtgI | CCRYGG | 2 cut(s) | 919, 1284 |
| BtgZI | GCGATG | 1 cut(s) | 959 |
| BtsCI | GGATG | 5 cut(s) | 18, 225, 889, 1216, 1332 |
| BtsIMutI | CAGTG | 1 cut(s) | 1186 |
| Cac8I | GCNNGC | 4 cut(s) | 163, 389, 759, 1148 |
| CfoI | GCGC | 6 cut(s) | 188, 233, 329, 848, 905, 918 |
| Cfr10I | RCCGGY | 2 cut(s) | 110, 428 |
| Cfr13I | GGNCC | 5 cut(s) | 199, 236, 288, 481, 601 |
| CpoI | CGGWCCG | 1 cut(s) | 481 |
| Csp6I | GTAC | 4 cut(s) | 106, 179, 841, 1273 |
| CspAI | ACCGGT | 2 cut(s) | 110, 428 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 860, 895 |
| CspI | CGGWCCG | 1 cut(s) | 481 |
| CviAII | CATG | 4 cut(s) | 196, 799, 920, 1243 |
| CviQI | GTAC | 4 cut(s) | 106, 179, 841, 1273 |
| DdeI | CTNAG | 2 cut(s) | 330, 372 |
| DpnI | GATC | 4 cut(s) | 747, 832, 912, 1251 |
| DpnII | GATC | 4 cut(s) | 745, 830, 910, 1249 |
| EaeI | YGGCCR | 3 cut(s) | 169, 244, 277 |
| EagI | CGGCCG | 1 cut(s) | 169 |
| Eam1104I | CTCTTC | 2 cut(s) | 1132, 1308 |
| EarI | CTCTTC | 2 cut(s) | 1132, 1308 |
| EciI | GGCGGA | 2 cut(s) | 438, 1059 |
| Ecl136II | GAGCTC | 1 cut(s) | 334 |
| EclXI | CGGCCG | 1 cut(s) | 169 |
| Eco105I | TACGTA | 1 cut(s) | 178 |
| Eco130I | CCWWGG | 1 cut(s) | 919 |
| Eco147I | AGGCCT | 1 cut(s) | 1178 |
| Eco24I | GRGCYC | 1 cut(s) | 336 |
| Eco47I | GGWCC | 3 cut(s) | 199, 481, 601 |
| Eco52I | CGGCCG | 1 cut(s) | 169 |
| Eco53kI | GAGCTC | 1 cut(s) | 334 |
| Eco57I | CTGAAG | 1 cut(s) | 619 |
| Eco88I | CYCGRG | 2 cut(s) | 203, 825 |
| EcoICRI | GAGCTC | 1 cut(s) | 334 |
| EcoO109I | RGGNCCY | 1 cut(s) | 236 |
| EcoRII | CCWGG | 3 cut(s) | 21, 208, 727 |
| EcoT14I | CCWWGG | 1 cut(s) | 919 |
| EcoT38I | GRGCYC | 1 cut(s) | 336 |
| ErhI | CCWWGG | 1 cut(s) | 919 |
| FaeI | CATG | 4 cut(s) | 199, 802, 923, 1246 |
| FaqI | GGGAC | 1 cut(s) | 842 |
| FatI | CATG | 4 cut(s) | 195, 798, 919, 1242 |
| FblI | GTMKAC | 2 cut(s) | 718, 1193 |
| Fnu4HI | GCNGC | 5 cut(s) | 166, 169, 836, 906, 1073 |
| FokI | GGATG | 5 cut(s) | 5, 212, 896, 1223, 1339 |
| FriOI | GRGCYC | 1 cut(s) | 336 |
| Fsp4HI | GCNGC | 5 cut(s) | 166, 169, 836, 906, 1073 |
| FspBI | CTAG | 1 cut(s) | 758 |
| FspI | TGCGCA | 2 cut(s) | 847, 904 |
| GlaI | GCGC | 6 cut(s) | 187, 232, 328, 847, 904, 917 |
| GluI | GCNGC | 5 cut(s) | 166, 169, 836, 906, 1073 |
| HaeII | RGCGCY | 1 cut(s) | 330 |
| HaeIII | GGCC | 7 cut(s) | 161, 171, 238, 246, 279, 290, 1178 |
| HapII | CCGG | 8 cut(s) | 99, 111, 260, 285, 291, 429, 479, 1197 |
| HhaI | GCGC | 6 cut(s) | 188, 233, 329, 848, 905, 918 |
| Hin1II | CATG | 4 cut(s) | 199, 802, 923, 1246 |
| Hin6I | GCGC | 6 cut(s) | 186, 231, 327, 846, 903, 916 |
| HinP1I | GCGC | 6 cut(s) | 186, 231, 327, 846, 903, 916 |
| HincII | GTYRAC | 2 cut(s) | 979, 1291 |
| HindII | GTYRAC | 2 cut(s) | 979, 1291 |
| HindIII | AAGCTT | 1 cut(s) | 661 |
| HinfI | GANTC | 5 cut(s) | 10, 341, 640, 676, 1033 |
| HpaII | CCGG | 8 cut(s) | 99, 111, 260, 285, 291, 429, 479, 1197 |
| HphI | GGTGA | 4 cut(s) | 151, 487, 560, 643 |
| Hpy166II | GTNNAC | 8 cut(s) | 108, 568, 719, 979, 1155, 1194, 1224, 1291 |
| Hpy188I | TCNGA | 7 cut(s) | 257, 519, 529, 684, 745, 819, 1349 |
| Hpy188III | TCNNGA | 5 cut(s) | 435, 479, 637, 690, 825 |
| Hpy8I | GTNNAC | 8 cut(s) | 108, 568, 719, 979, 1155, 1194, 1224, 1291 |
| Hpy99I | CGWCG | 2 cut(s) | 80, 539 |
| HpyAV | CCTTC | 5 cut(s) | 317, 508, 514, 1168, 1316 |
| HpyCH4III | ACNGT | 4 cut(s) | 380, 535, 1272, 1288 |
| HpyCH4IV | ACGT | 1 cut(s) | 177 |
| HpyCH4V | TGCA | 6 cut(s) | 16, 391, 812, 887, 1000, 1186 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 922, 963 |
| HpyF3I | CTNAG | 2 cut(s) | 330, 372 |
| HpySE526I | ACGT | 1 cut(s) | 177 |
| Hsp92II | CATG | 4 cut(s) | 199, 802, 923, 1246 |
| HspAI | GCGC | 6 cut(s) | 186, 231, 327, 846, 903, 916 |
| Kpn2I | TCCGGA | 1 cut(s) | 478 |
| Kzo9I | GATC | 4 cut(s) | 745, 830, 910, 1249 |
| LmnI | GCTCC | 2 cut(s) | 50, 541 |
| Lsp1109I | GCAGC | 2 cut(s) | 847, 917 |
| LweI | GCATC | 5 cut(s) | 234, 480, 874, 947, 1270 |
| MaeI | CTAG | 1 cut(s) | 758 |
| MaeII | ACGT | 1 cut(s) | 177 |
| MaeIII | GTNAC | 7 cut(s) | 113, 173, 407, 706, 926, 1061, 1238 |
| MalI | GATC | 4 cut(s) | 747, 832, 912, 1251 |
| MbiI | CCGCTC | 1 cut(s) | 702 |
| MboI | GATC | 4 cut(s) | 745, 830, 910, 1249 |
| MboII | GAAGA | 7 cut(s) | 482, 787, 1083, 1149, 1307, 1325, 1331 |
| MfeI | CAATTG | 2 cut(s) | 298, 734 |
| MflI | RGATCY | 2 cut(s) | 745, 910 |
| MhlI | GDGCHC | 5 cut(s) | 84, 195, 336, 351, 968 |
| MlsI | TGGCCA | 2 cut(s) | 246, 279 |
| MluCI | AATT | 5 cut(s) | 298, 473, 734, 766, 1108 |
| MluNI | TGGCCA | 2 cut(s) | 246, 279 |
| MlyI | GAGTC | 1 cut(s) | 634 |
| MmeI | TCCRAC | 2 cut(s) | 111, 280 |
| Mox20I | TGGCCA | 2 cut(s) | 246, 279 |
| MroI | TCCGGA | 1 cut(s) | 478 |
| MroXI | GAANNNNTTC | 2 cut(s) | 430, 474 |
| MscI | TGGCCA | 2 cut(s) | 246, 279 |
| MseI | TTAA | 2 cut(s) | 645, 770 |
| MslI | CAYNNNNRTG | 2 cut(s) | 536, 1160 |
| Msp20I | TGGCCA | 2 cut(s) | 246, 279 |
| MspA1I | CMGCKG | 1 cut(s) | 165 |
| MspCI | CTTAAG | 1 cut(s) | 644 |
| MspI | CCGG | 8 cut(s) | 99, 111, 260, 285, 291, 429, 479, 1197 |
| MspR9I | CCNGG | 5 cut(s) | 23, 100, 210, 286, 729 |
| MunI | CAATTG | 2 cut(s) | 298, 734 |
| MvaI | CCWGG | 3 cut(s) | 23, 210, 729 |
| MvnI | CGCG | 4 cut(s) | 186, 188, 231, 916 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 922, 963 |
| NciI | CCSGG | 2 cut(s) | 100, 286 |
| NcoI | CCATGG | 1 cut(s) | 919 |
| NdeII | GATC | 4 cut(s) | 745, 830, 910, 1249 |
| NheI | GCTAGC | 1 cut(s) | 757 |
| NlaIII | CATG | 4 cut(s) | 199, 802, 923, 1246 |
| NlaIV | GGNNCC | 5 cut(s) | 41, 52, 289, 427, 543 |
| NmuCI | GTSAC | 2 cut(s) | 407, 1061 |
| NsbI | TGCGCA | 2 cut(s) | 847, 904 |
| NspV | TTCGAA | 1 cut(s) | 1135 |
| OliI | CACNNNNGTG | 1 cut(s) | 536 |
| PaeR7I | CTCGAG | 2 cut(s) | 203, 825 |
| PceI | AGGCCT | 1 cut(s) | 1178 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1299 |
| PdmI | GAANNNNTTC | 2 cut(s) | 430, 474 |
| PfeI | GAWTC | 4 cut(s) | 10, 341, 676, 1033 |
| PflFI | GACNNNGTC | 1 cut(s) | 533 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 60, 665 |
| PinAI | ACCGGT | 2 cut(s) | 110, 428 |
| PkrI | GCNGC | 5 cut(s) | 167, 170, 837, 907, 1074 |
| PleI | GAGTC | 1 cut(s) | 634 |
| PpsI | GAGTC | 1 cut(s) | 634 |
| Ppu21I | YACGTR | 1 cut(s) | 178 |
| PsiI | TTATAA | 1 cut(s) | 1085 |
| Psp124BI | GAGCTC | 1 cut(s) | 336 |
| Psp6I | CCWGG | 3 cut(s) | 21, 208, 727 |
| PspGI | CCWGG | 3 cut(s) | 21, 208, 727 |
| PspN4I | GGNNCC | 5 cut(s) | 41, 52, 289, 427, 543 |
| PspPI | GGNCC | 5 cut(s) | 199, 236, 288, 481, 601 |
| PspXI | VCTCGAGB | 1 cut(s) | 203 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1001, 1033 |
| PsuI | RGATCY | 2 cut(s) | 745, 910 |
| PsyI | GACNNNGTC | 1 cut(s) | 533 |
| RsaI | GTAC | 4 cut(s) | 107, 180, 842, 1274 |
| RsaNI | GTAC | 4 cut(s) | 106, 179, 841, 1273 |
| RseI | CAYNNNNRTG | 2 cut(s) | 536, 1160 |
| Rsr2I | CGGWCCG | 1 cut(s) | 481 |
| RsrII | CGGWCCG | 1 cut(s) | 481 |
| SacI | GAGCTC | 1 cut(s) | 336 |
| SaqAI | TTAA | 2 cut(s) | 645, 770 |
| SatI | GCNGC | 5 cut(s) | 166, 169, 836, 906, 1073 |
| Sau3AI | GATC | 4 cut(s) | 745, 830, 910, 1249 |
| Sau96I | GGNCC | 5 cut(s) | 199, 236, 288, 481, 601 |
| ScaI | AGTACT | 1 cut(s) | 842 |
| SchI | GAGTC | 1 cut(s) | 634 |
| ScrFI | CCNGG | 5 cut(s) | 23, 100, 210, 286, 729 |
| SduI | GDGCHC | 5 cut(s) | 84, 195, 336, 351, 968 |
| SfaNI | GCATC | 5 cut(s) | 234, 480, 874, 947, 1270 |
| Sfr274I | CTCGAG | 2 cut(s) | 203, 825 |
| SfuI | TTCGAA | 1 cut(s) | 1135 |
| SinI | GGWCC | 3 cut(s) | 199, 481, 601 |
| SlaI | CTCGAG | 2 cut(s) | 203, 825 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 536, 1160 |
| SmlI | CTYRAG | 3 cut(s) | 203, 644, 825 |
| SmoI | CTYRAG | 3 cut(s) | 203, 644, 825 |
| SnaBI | TACGTA | 1 cut(s) | 178 |
| Sse9I | AATT | 5 cut(s) | 298, 473, 734, 766, 1108 |
| SseBI | AGGCCT | 1 cut(s) | 1178 |
| SspMI | CTAG | 1 cut(s) | 758 |
| SstI | GAGCTC | 1 cut(s) | 336 |
| StuI | AGGCCT | 1 cut(s) | 1178 |
| StyD4I | CCNGG | 5 cut(s) | 21, 98, 208, 284, 727 |
| StyI | CCWWGG | 1 cut(s) | 919 |
| TaaI | ACNGT | 4 cut(s) | 380, 535, 1272, 1288 |
| TaiI | ACGT | 1 cut(s) | 180 |
| TaqI | TCGA | 9 cut(s) | 204, 351, 606, 784, 826, 952, 1094, 1112, 1135 |
| TaqII | GACCGA | 1 cut(s) | 498 |
| TasI | AATT | 5 cut(s) | 298, 473, 734, 766, 1108 |
| TatI | WGTACW | 2 cut(s) | 105, 840 |
| TauI | GCSGC | 3 cut(s) | 168, 171, 1075 |
| TfiI | GAWTC | 4 cut(s) | 10, 341, 676, 1033 |
| Tru1I | TTAA | 2 cut(s) | 645, 770 |
| Tru9I | TTAA | 2 cut(s) | 645, 770 |
| TscAI | CASTG | 1 cut(s) | 1193 |
| TseFI | GTSAC | 2 cut(s) | 407, 1061 |
| TseI | GCWGC | 2 cut(s) | 835, 905 |
| Tsp45I | GTSAC | 2 cut(s) | 407, 1061 |
| TspDTI | ATGAA | 5 cut(s) | 606, 627, 787, 1007, 1259 |
| TspGWI | ACGGA | 4 cut(s) | 335, 553, 844, 1241 |
| TspRI | CASTG | 1 cut(s) | 1193 |
| Tth111I | GACNNNGTC | 1 cut(s) | 533 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 60, 665 |
| Vha464I | CTTAAG | 1 cut(s) | 644 |
| VpaK11BI | GGWCC | 3 cut(s) | 199, 481, 601 |
| XapI | RAATTY | 3 cut(s) | 473, 766, 1108 |
| XhoI | CTCGAG | 2 cut(s) | 203, 825 |
| XmiI | GTMKAC | 2 cut(s) | 718, 1193 |
| XmnI | GAANNNNTTC | 2 cut(s) | 430, 474 |
| XspI | CTAG | 1 cut(s) | 758 |
| ZrmI | AGTACT | 1 cut(s) | 842 |