pycom07g00020

Beta-hexosaminidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009312
Physical Location & Seq
Reverse (-)
14079 .. 14309
231 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 231 bp
ATGCCTGGCTGTTGGAAAGCTGATCCAGCCATTCAATCGTTTCTATCAAAGGGTGGAACGCTCAGTGAGCTTCTTGAGCTTTTTGTCAACTCCACTTTCCCTCACATTATATCTCTTAATAGGACGGTGGTTTATTGGGAAGACATTTTGCTACATGATAATGTCAAGGTGCAAGCACTCTGGATACCGCGTTATTGTGTCATCCTCGGAGTTCTATTACTTGGATTGTGA

Protein Analysis

77

Amino Acids

8.57

Weight (kDa)

6.0

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_20 PF00728 3 - 55 9.4e-06 Glycosyl hydrolase family 20, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 190
AciI CCGC 1 cut(s) 188
AclWI GGATC 1 cut(s) 17
AgsI TTSAA 1 cut(s) 35
AjnI CCWGG 1 cut(s) 4
AluBI AGCT 3 cut(s) 20, 70, 79
AluI AGCT 3 cut(s) 20, 70, 79
AlwI GGATC 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 147
BciT130I CCWGG 1 cut(s) 6
BciVI GTATCC 1 cut(s) 177
BfuI GTATCC 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 205
BseBI CCWGG 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 201
BseMII CTCAG 1 cut(s) 76
Bsh1236I CGCG 1 cut(s) 190
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 1 cut(s) 188
BspCNI CTCAG 1 cut(s) 75
BspFNI CGCG 1 cut(s) 190
BspPI GGATC 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 1 cut(s) 22
Bst2UI CCWGG 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 1 cut(s) 62
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 1 cut(s) 190
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 3 cut(s) 26, 67, 76
BstNI CCWGG 1 cut(s) 6
BstSCI CCNGG 1 cut(s) 4
BstUI CGCG 1 cut(s) 190
BstV2I GAAGAC 1 cut(s) 147
BsuI GTATCC 1 cut(s) 177
BtsCI GGATG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 70
Cac8I GCNNGC 1 cut(s) 174
CviAII CATG 1 cut(s) 155
CviJI RGCY 5 cut(s) 9, 20, 29, 70, 79
CviKI_1 RGCY 5 cut(s) 9, 20, 29, 70, 79
DdeI CTNAG 1 cut(s) 62
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 4
FaeI CATG 1 cut(s) 158
FaiI YATR 2 cut(s) 110, 156
FatI CATG 1 cut(s) 154
FokI GGATG 1 cut(s) 188
Hin1II CATG 1 cut(s) 158
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 2 cut(s) 74, 181
Hpy8I GTNNAC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 67, 76
HpyF3I CTNAG 1 cut(s) 62
Hsp92II CATG 1 cut(s) 158
Kzo9I GATC 1 cut(s) 22
LpnPI CCDG 3 cut(s) 18, 39, 166
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 1 cut(s) 152
MnlI CCTC 2 cut(s) 111, 215
MseI TTAA 1 cut(s) 117
MslI CAYNNNNRTG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 6
MvaI CCWGG 1 cut(s) 6
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 3 cut(s) 26, 67, 76
NdeII GATC 1 cut(s) 22
NlaIII CATG 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 4
PspGI CCWGG 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 1 cut(s) 117
Sau3AI GATC 1 cut(s) 22
ScrFI CCNGG 1 cut(s) 6
SetI ASST 4 cut(s) 22, 72, 81, 171
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SsiI CCGC 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 4
TaaI ACNGT 1 cut(s) 127
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 70
TspRI CASTG 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.